Task 3. Create A Triggered Dynamic Mrm Method From An Existing Database - Agilent Technologies 6460 Manual

Task 3. Create a Triggered Dynamic MRM method from an existing
database
You can create a Triggered Dynamic MRM method from a database such as the
Pesticide Triggered MRM Database and Library, the Forensics and
Toxicology Triggered MRM Database and Library, or the Veterinary Drug
Triggered MRM Database and Library. These databases can be purchased
from Agilent. You can also copy the information from an Excel spreadsheet,
but that method is not described in this guide.
Steps
1 In the Data Acquisition program,
you now import the updated
compounds from the database.
These compounds have optimized
collision energies and also Primary
and Trigger transitions marked.
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Exercise 3 – Create a Triggered Dynamic MRM acquisition method

Task 3. Create a Triggered Dynamic MRM method from an existing database

Detailed Instructions
a Switch to the Data Acquisition
program.
b Open the iiiSulfamix_dMRM2.m
method.
c In the QQQ tab, click the Acquisition
tab. The Scan segments table
contains four rows which are deleted
later.
d Right-click the Scan Segments table
and click Import from Database
Browser. The Database Browser
program opens.
e Mark the Show All Records check
box.
f Mark all of the transitions for the four
sulfa drug compounds. Clear the
check boxes next to any unwanted
compounds.
g Click the Add to Import List button.
h Click the Import List tab.
i Review the Import List table.
j Click the Import button.
k Delete the original compounds from
the Scan segments table.
l Mark the Triggered check box under
Triggered MRM.
Comments
• Before you import compounds from
Database Browser, the Scan
segments table contains at least
one row. After importing
compounds from the Database
Browser, you need to remove any
original rows.
• The Scan segments table always
has to have at least one row.
• The triggering information is loaded
from the Database Browser
program even if the Triggered check
box is clear.
• See the online Help for the Data
Acquisition program and the QQQ
Concepts Guide for an explanation
of the other triggering conditions:
Trigger Entrance, Trigger Delay,
and Trigger Window.
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