Task 3. Find Optimum Fragmentor Voltage For Maximum Response - Agilent Technologies 6460 Manual

Exercise 1 – Develop an acquisition method

Task 3. Find optimum fragmentor voltage for maximum response

Task 3. Find optimum fragmentor voltage for maximum response
Task 3 shows you how to carry out the optimization for fragmentor voltage by
creating selected ion-monitoring experiments for each compound within a
method and setting up multiple methods with varying fragmentor voltages.
Steps
1 Set up six methods for six different
fragmentor voltages.
• Change to a SIM experiment.
• Use 60, 80, 100, 140, 180 and
220 volts as the fragmentor
voltages for the six methods.
• Save the methods as
iiiMS2SIMxxx.m, where iii are
your initials and xxx is the
voltage.
14
Detailed Instructions
a In the Scan Type dropdown list, click
MS2 SIM.
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
Comments