Extracellular Voltage Measurement - NPI ELC-03XS Operating Instructions And System Description

ELC-03XS User Manual
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Figure 11: headstage connections, A: differential measurement, B: single-ended measurement
8.2.

Extracellular Voltage Measurement

Extracellular measurements are usually done in the loose-patch configuration or with special
metal microelectrodes. Recordings with extracellular metal electrodes is simple. The electrode
is advanced into the region where the recordings will be made using a micromanipulator and
the signals are filtered and amplified (see chapter 5 in Lalley et al., 1999 for details) as
required. For loose patch recording the basic procedure is the following (Barbour & Isope,
2000, Nunemaker et al, 2003):
 Approach the cell in VC mode and apply square voltage pulses to the electrode.
 Contact the cell and establish the loose-patch.
 Set the MODE OF OPERATION switch to OFF.
 Set the required amplification of the POTENTIAL OUTPUT.
 Set the HIGHPASS FILTER to the desired corner frequency, e.g. 0.3 Hz.
 Set the LOWPASS FILTER to the desired corner frequency, e.g. 3 kHz.
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