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Related Manuals for NPI VA-10X
Summary of Contents for NPI VA-10X
- Page 1 OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION OF THE VA-10X VOLTAMMETRIC AND AMPEROMETRIC AMPLIFIER VERSION 7.0 npi 2014 npi electronic GmbH, Bauhofring 16, D-71732 Tamm, Germany Phone +49 (0)7141-9730230; Fax: +49 (0)7141-9730240 support@npielectronic.com; http://www.npielectronic.com...
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Page 2: Table Of Contents
6.1. Setting up the VA-10X Voltammeter ................12 6.2. Testing Basic Functions of the VA-10X ..............14 Open Circuit Test ......................14 DC Accuracy ....................... 14 Dynamic Test / Frequency Response ................15 ... -
Page 3: Safety Regulations
General safety regulations for operating electrical devices are to be followed. AC MAINS CONNECTION: While working with the npi systems, always adhere to the appropriate safety measures for handling electronic devices. Before using any device please read manuals and instructions carefully. -
Page 4: Introduction
VA-10X User Manual _______________________________________________________________________________________________________________ 2. Introduction Recently, electrochemical methods using carbon-fiber microelectrodes have been applied to measure the release of oxidizable transmitter from single cells, and, even more impressively, from single exocytotic vesicles. Transmitters that are oxidizable and which, therefore, can be measured with this approach, include serotonin, dopamine, adrenaline, and noradrenaline. -
Page 5: Va-10X Components
(e.g. for cyclic voltammetry). The VA-10X is ideally suited for measurements from single cells plated onto glass cover slips and with carbon-fiber disk electrodes having diameters of 10 µM or less. However, it can also be used for measurements made on superficially located cells in tissue slices. - Page 6 VA-10X User Manual _______________________________________________________________________________________________________________ Figure 1: VA-10X Front panel view ___________________________________________________________________________ version 7.0 page 6...
- Page 7 FILTERED connector and (15) OUTPUT UNFILTERED connector (3) - OVER + OVER LED The VA-10X has an over voltage indicator with two LEDs, which light up if the amplifier is near it’s positive or negative limit (±10 V). (14) OUTPUT FILTERED connector The OUTPUT FILTERED BNC connector provides a voltage that is proportional to the current passed through the electrode (mV/pA with GAIN 1).
- Page 8 VA-10X User Manual _______________________________________________________________________________________________________________ INPUT unit The INPUT unit consists of (5) INPUT coupling switch and (10) INPUT ÷10 mV connector. (5) INPUT coupling switch The switch must be set to ON, if an external waveform is used. If no external waveform is used, the switch should be set to OFF to avoid coupling of noise.
- Page 9 VA-10X User Manual _______________________________________________________________________________________________________________ MONITORING unit The MONITORING unit consists of (11) COMMAND MONITOR connector, (12) FILTER MONITOR connector and (13) GAIN MONITOR connector. (11) COMMAND MONITOR x10 mV connector The COMMAND MONITOR voltage provided at this BNC connector is the voltage at the electrode multiplied by a factor of 10, i.e.
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Page 10: Headstage
VA-10X User Manual _______________________________________________________________________________________________________________ 5. Headstage The VA-10X comes with the standard headstage (range: 1000 mV) for connecting carbon- fiber electrodes via an electrode holder (optional). A 3-electrode headstage with differential input (see also Optional accessories in chapter 3, and chapter 5.2) is also available. For details contact npi. -
Page 11: 3-Electrode Headstage (Optional)
Important: If REF is not used, REF must be connected to GROUND. Figure 3: VA-10X 3 electrode headstage with CFE electrode holder (optional) Reference for typical application: Marinesco, S. and Carew, T. J. (2002). Serotonin Release Evoked by Tail Nerve Stimulation in the CNS of Aplysia: Characterization and Relationship to Heterosynaptic Plasticity. -
Page 12: Operation
6. Operation 6.1. Setting up the VA-10X Voltammeter The VA-10X amplifier is shipped as a stand alone system and equipped with a small headstage with a BNC connector. When the system arrives the headstage will not be connected to the cabinet. - Page 13 VA-10X User Manual _______________________________________________________________________________________________________________ If you intend to read the signal from the VA-10X into a data acquisition system: connect a BNC cable from the data acquisition system to the FILTERED or UNFILTERED connector (#14 or #15, Figure 1). Additionally, you can monitor the...
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Page 14: Testing Basic Functions Of The Va-10X
VA-10X User Manual _______________________________________________________________________________________________________________ 6.2. Testing Basic Functions of the VA-10X All tests should be made in a noise free environment (e.g. Faraday cage or metal box connected to GROUND). Please be careful, the headstage is sensitive to electrostatic discharges (see also chapter 1). Please note that the headstage enclosure is NOT connected to GROUND, it is connected to the COMMAND signal applied to the microelectrode. -
Page 15: Dynamic Test / Frequency Response
These electrodes can be purchased or you can make your own. For use with the VA-10X voltammeter the electrodes must fit to the BNC connector at the input of the headstage. Two types of connection are commonly used:... -
Page 16: Counter Electrode
For this application it is necessary to connect an external voltage source to the INPUT ÷10 mV connector (#10, Figure 1) at the front panel of the VA-10X. Because one has to relate the measured current to the applied instantaneous voltage, the current and the applied voltage should be recorded simultaneously with a data acquisition system. -
Page 17: Literature
VA-10X User Manual _______________________________________________________________________________________________________________ 7. Literature VA-10 Typical recordings Bai, J., Wang, C. T., Richards, D. A., Jackson, M. B., & Chapman, E. R. (2004). Fusion pore dynamics are regulated by synaptotagmin*t-SNARE interactions. Neuron 41, 929-942. Barclay, J. W., Craig, T. J., Fisher, R. J., Ciufo, L. F., Evans, G. J., Morgan, A., &... - Page 18 VA-10X User Manual _______________________________________________________________________________________________________________ Han, X. & Jackson, M. B. (2005). Electrostatic Interactions between the Syntaxin Membrane Anchor and Neurotransmitter Passing through the Fusion Pore. Biophys.J. 88, L20-L22. Jaffe, E. H, Marty, A., Schulte, A. and Chow, R.H. (1998). Extrasynaptic vesicular transmitter release from the somata of substantia nigra neurons in rat midbrain slices.
- Page 19 VA-10X User Manual _______________________________________________________________________________________________________________ VA-10 USED FOR RECORDINGS WITH 3 ELECTRODES Marinesco, S., & Carew, T. J. (2002). Serotonin Release Evoked by Tail Nerve Stimulation in the CNS of Aplysia: Characterization and Relationship to Heterosynaptic Plasticity. J.Neurosci. 22, 2299–2312.
- Page 20 VA-10X User Manual _______________________________________________________________________________________________________________ REFERENCES (METHODS) Alvarez de Toledo, G., Fernandez-Chacon, R., & Fernandez, J. M. (1993). Release of secretory products during transient vesicle fusion. Nature 363, 554-557. Britt, J. P. & McGehee, D. S. (2008). Presynaptic opioid and nicotinic receptor modulation of dopamine overflow in the nucleus accumbens.
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Page 21: Technical Data
VA-10X User Manual _______________________________________________________________________________________________________________ 8. Technical Data Headstage: Input voltage range: ±1200 mV Operating voltage: ±12 V Enclosure: Size: 40 x 70 x 20 mm, driven shield (COMMAND potential) mounting plate: 50 x 70 mm, not conducting Electrode connector: BNC with shield (COMMAND potential)
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