Match Selector Dial With The Low-Power Objective; Replace Eyepiece With Phase-Centering Telescope; Focus The Phase-Centering Telescope; Figure 1 - Set Condenser Dial To Match The Objective - Olympus BH-2 Manual

• Olympus IF-550 (interference) or 45G533 (absorptive) filter
• One or more Olympus LB phase contrast objectives

Match Selector Dial with the Low-Power Objective

Select the lowest-powered phase contrast objective
available on your microscope. Rotate the selector dial
on the front of the phase contrast condenser until the
number visible on the front of the dial matches the
selected phase contrast objective (see
sure the selected number on the dial clicks in place.
Figure 1 – Set condenser dial to match the objective

Replace Eyepiece with Phase-Centering Telescope

Carefully remove the eyepiece from the right-hand
ocular tube and insert an Olympus phase-centering
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telescope in its place (see
Figure 2 – Replace right eyepiece with phase telescope

Focus the Phase-Centering Telescope

Look into the phase-centering telescope.
lighting intensity set to a comfortable viewing level, you
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Use the "CT-5" for 23mm tubes and use the "CT-30" for 30mm
tubes. Note that other manufacturer's centering telescopes may be
substituted for the Olympus CT, so long as the barrel diameter of the
phase telescope is compatible with your ocular tubes.
Phase Contrast on the Olympus BH-2 Microscopes
Figure 1). Make
Figure 2).
With the
should see a bright ring of light and also a darker ring,
similar to that shown in
may be so blurry that these features cannot be readily
discerned at this point. Also note that the relative
positioning of the two rings may vary from that shown
in Figure 3.
Figure 3 – Phase telescope improperly focused
Rotate the knurled focusing ring on the phase-centering
telescope (see Figure 4) until the image seen through
the phase-centering telescope is sharply focused (see
Figure 5).
Figure 4 – Adjust knurled focus ring to focus the image
Figure 5 – The phase telescope is properly focused
Revision 1
Figure 3. Note that the image
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