Condenser - Olympus BX51 Instructions Manual

Aperture iris
diaphragm image
Objective pupil
Fig. 31
N.A. scale
@
Fig. 32
2
Compatibility of Objectives and Condensers
Objective
Magnification
1.25X
2X
4X Applicable to FN 22
10-60X
100X
* When using the U-SC3 swing-out condenser together with a 1.25X to 4X objective, fully open the condenser aperture iris
diaphragm and use the field iris diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the
surroundings of the field of view may become dark.
}To obtain better illumination in photomicrography using a 1.25X to 4X objective, use of the U-ULC-2 is recommended.
Aperture Iris Diaphragm (Figs. 31 & 32)
70-80%
· The aperture iris diaphragm determines the numerical aperture of the
30-20%
illumination system. Matching the numerical aperture of the illumination
system with that of the objective provides better image resolution and
contrast, and also increases the depth of focus.
· Since the contrast of microscope specimens is ordinarily low, setting the
condenser aperture iris diaphragm to between 70% and 80% of the N.A.
of the objective in use is usually recommended. If necessary, adjust the
ratio by removing the eyepiece and looking into the eyepiece sleeve
while adjusting the aperture iris diaphragm ring @ until the image shown
in Fig. 31 is seen.
}Using the numerical aperture scale:
Set the condenser numerical aperture scale to about 80% of the NA
value ² of the respective objective. (Fig. 32)
²
Example: With the UPlanFI40X (NA 0.75), set the scale to 0.75 x 0.8 = 0.6.
Abbe Achromat/aplanat
U-AC2 U-AAC
Applicable Applicable
(FN 26.5) (FN 26.5)

Condenser

Swing-out
U-SC3
Applicable to FN 22
Applicable (FN 26.5) by
moving top lens out of
the light path.* (FN 26.5)
Engage top lens in
light path (FN 26.5)
BX51/BX52
Ultralow magnification
U-ULC-2
Applicable
(FN 26.5)
19