Using A Preloaded Pcr Program - Agilent Technologies SureCycler 8800 Setup And User Manual

Using a Preloaded PCR Program

Several PCR programs come preloaded into the software for use on the
96- well SureCycler 8800. These programs were designed for performing
PCR with a variety of Agilent PCR enzymes and kits to amplify human
genomic DNA or vector DNA.
Each preloaded program is optimized to amplify targets of a particular
length with an annealing temperature that is suitable for some primers.
You may need to adjust the annealing temperature, the elongation time or
another cycling parameter used in the preloaded program to better suit
your particular target of interest. Any of the preloaded programs can be
saved under a new file name and then edited to optimize the program for
your primer/template system.
The available preloaded programs are listed in
Table 5 Description of the preloaded PCR programs for the 96-well SureCycler 8800
PCR Program File Name
Herculase II Fusion 1kb target.pcr
Paq5000 HS MM 1kb target.pcr
PfuTurbo HS MM 1kb target.pcr
PfuUltra II HS MM 1kb target.pcr
QuikChange II 4 kb target.pcr
QuikChange Lightning 4kb target.pcr
QuikChange Lighting Multi 4 kb
target.pcr
QuikChange Multi 4kb target.pcr
Agilent SureCycler 8800 Setup and User's Guide
PCR Enzyme or Kit
Herculase II Fusion DNA Polymerase 95°C for
Paq5000 Hotstart Master Mix
PfuTurbo Hotstart Master Mix
PfuUltra II Hotstart Master Mix
QuikChange II Site-Directed
Mutagenesis Kit
QuikChange Lightning Site-Directed
Mutagenesis Kit
QuikChange Lightning Multi
Site-Directed Mutagenesis Kit
QuikChange Multi Site-Directed
Mutagenesis Kit
PCR Programs

Using a Preloaded PCR Program

Table 5.
Denaturation Annealing
55°C for
20 seconds 20 seconds
95°C for 55°C for
20 seconds 20 seconds
95°C for 55°C for
30 seconds 30 seconds
95°C for 55°C for
20 seconds 20 seconds
95°C for 55°C for
30 seconds 1 minute
95°C for 60°C for
20 seconds 10 seconds
95°C for 55°C for
20 seconds 30 seconds
95°C for 55°C for
1 minute 1 minute
4
Elongation
72°C for
30 seconds
72°C for
30 seconds
72°C for
1 minute
72°C for
15 seconds
68°C for
4 minutes
68°C for
2 minutes
65°C for
2 minutes
65°C for
8 minutes
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