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4
PCR Programs
Storage/Pause Step
The difference between the maximum and minimum annealing temperatures is commonly
N O T E
between 5°C and 10°C starting with a maximum temperature that is 2°C above the higher
primer melting temperature (T
Primer 2 is 54°C, set the max temperature to 62°C and the min temperature to around 52°C.
Hold For
Enter the duration of the annealing step.
Elongation
Enter the elongation temperature (in the Elongation Temperature field)
Temperature and
and the duration of elongation (in the adjacent Hold For field) to be used
Hold Time
during touchdown cycling.
Cycles
Enter the number of cycles for the Touchdown step.
Storage/Pause Step
The Storage/Pause program step cools the thermal block down to a
temperature between 4° and 12°C for a specified period of time or
indefinitely.
Hold Temperature
Enter the storage temperature in °C.
Hold for
Select this radio button if you prefer to maintain the temperature for a set
duration of time. Enter the duration in the accompanying field.
Infinite
Select this radio button to hold the specified store temperature for an
unlimited period of time.
50
). For example, if the T
m
Agilent SureCycler 8800 Setup and User's Guide
of Primer 1 is 60°C and the T
m
of
m
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