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4
PCR Programs
Tips for Creating PCR Programs for the 384-well SureCycler 8800
Tips for Creating PCR Programs for the 384-well SureCycler 8800
When performing PCR on the 384- well block, reducing the duration of the
denaturation step during cycling may improve product yield for certain
enzymes. Denaturation times as short as 3–5 seconds may be optimal in
some protocols.
If you use the PCR Wizard to design a PCR, the program will need to be
edited after program creation to shorten the denaturation temperature
step. See
on program editing.
42
"To edit program step parameters"
on page 53 for instructions
Agilent SureCycler 8800 Setup and User's Guide
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