Cycle Wizard - Agilent Technologies SureCycler 8800 Setup And User Manual

4 PCR Programs

Cycle Wizard

Cycle Wizard
The Cycle Wizard command can be used to create the steps of the
program that are to be cycled multiple times. Using the Cycle Wizard to
create cycled steps replaces the need to manually add Start Cycle and End
Cycle commands. These steps will automatically be added to the program
by the wizard.
Denaturation Enter the denaturation temperature in °C (left field) and denaturation
Step time in hours : minutes : seconds (right field).
Annealing Step Enter the annealing temperature in °C (left field) and annealing time in
hours : minutes : seconds (right field).
Elongation Step If your PCR protocol uses 3- step cycling with separate annealing and
elongation steps (rather than a 2- step cycling protocol with a combined
annealing/elongation step), mark the check box labeled Use 3rd step.
Enter the elongation temperature in °C (left field) and elongation time in
hours : minutes : seconds (right field).
Cycles Enter the number of cycles.
44 Agilent SureCycler 8800 Setup and User's Guide