Agilent Technologies SureCycler 8800 Setup And User Manual page 48

4 PCR Programs
Gradient Step
During the run, the instrument generates a temperature gradient based on
the values entered into the Left Side Temperature Gradient and Right
Side Temperature Gradient fields. The difference between the minimum
and maximum temperatures must not exceed 30°C.
Left Side
Enter the desired temperature for the left side of the thermal block.
Temperature
Gradient
Right Side
Enter the desired temperature for the right side of the thermal block.
Temperature
Gradient
Hold for
Enter the duration of the Gradient Step (units are minutes : seconds).
Convert to
Once the optimal annealing temperature has been determined, the
Temperature Step
Gradient Step can be easily converted to a standard Temperature Step by
(optional)
marking this check box and specifying in the drop- down which
temperature column yielded the best amplification results. The Gradient
Step will then automatically be converted to a standard Temperature Step
in which the temperature of the thermal block is uniform.
48 Agilent SureCycler 8800 Setup and User's Guide