Agilent Technologies SureCycler 8800 Setup And User Manual page 20

3 Getting Started
Sample Preparation and Loading
Sample Preparation and Loading
To prevent damage to the block and the heated lid, use only
temperature- stable sample tubes and plates. Unsuitable tubes and plates
can become damaged during cycling. See
Consumables"
Danger of Burns: The thermal block, sample tubes and plates may reach
WA R N I N G
temperatures as high as 100°C. Keep hands away until temperature is 30°C or less.
To prepare samples
Keep the following guidelines in mind when preparing samples in reaction
plates and tubes:
• Place caps on tubes before loading samples into the thermal block.
• For best results, just before loading the samples into the thermal block,
• If using sealing film to cover a reaction plate, use a compression mat
To load samples
The instrument can be loaded with individual PCR tubes or strip tubes or,
depending upon the thermal block installed, one 96- well PCR plate or one
384- well PCR plate.
1 Lift the lid of the thermal block by pressing firmly on the button on the
2 Put your plate or tubes on the block and check that they are correctly
3 Close the lid until it latches into place.
20
on page 21 for a list of recommended plasticware.
spin them briefly in a centrifuge.
during cycling to ensure the film stays firmly adhered to the plate. See
"Agilent Plasticware and Consumables"
information on a compression mat.
top of the instrument to unlatch the lid.
positioned.
On the 96- well block, seat the tubes in the round holes on the thermal
block. On the 384- well block, seat the tubes in the larger round holes.
"Agilent Plasticware and
on page 21 for ordering
Agilent SureCycler 8800 Setup and User's Guide