Koehler Illumination - Leica DM IRE2 Manual

Koehler illumination

Koehler illumination is essential to obtain good
transmitted light images
 Select objective
 Open the condenser aperture diaphragm
(move lever to the right)
 Focus on specimen
 Close the field aperture diaphragm
(move lever to left)
 Focus condenser using condenser focusing
knob until the image of the aperture is sharp
 If necessary center the field diaphragm in the field
of view using the two centering screws located
on the front of the condenser
 Open field aperture diaphragm until it just
disappears from the field of view
 Close down the condenser aperture diaphragm
until the desired contrast is achieved
Field aperture
diaphragm
Condenser
focusing knob
DIC polarizer
Condenser
turret
Phase ring centering
keys insert here
19
centering
screws
Condenser
aperture
diaphragm
CMN Imaging Core
January 16, 2013