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Summary of Contents for Leica DMi8A
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Page 1: Leica Dmi8A Quick Guide
Leica DMi8A Quick Guide ... -
Page 2: Table Of Contents
Optical Microscope Quick Start Guide The following instructions are provided as a Quick Start Guide for powering up, running measurements, and shutting down Leica’s DMi8A Inverted Optical Microscope. Warning: Although optical microscopy has no inherent danger to usage, only users who have received training by qualified department staff or faculty are permitted to use this equipment. Contact the DMSE for training information. Notice: Italicized words indicate external buttons in the real world. Bolded words indicate buttons within the computer system. Table of Contents Leica DMi8A Quick Guide ...................... -
Page 3: Instrument Information
Physical Setup 1) Turn on the optical microscope then turn on the computer a. The power switch is on the CTR advanced box 2) Wait a few moments for the DMi8A to self-align and go through its boot-up sequence Computer Setup 1) Double click on the LAS V4.7 computer icon... -
Page 4: Operating The Microscope
Can be done on computer w/ Mic2 c. Can be done w/ manual stage adjustment 2) Adjust the Zoom Levels on the microscope a. Available magnifications: 5x, 10x, 25x, 50x, 100x Figure: The available magnifications for the DMi8A. The selected magnification is highlighted orange. Adjusting the Filtering Parameters Figure: The X‐Y‐Z Controller for the Depending on the properties of your sample and its environment, microscope. The larger two knobs ... - Page 5 Dark Field ꞏWell suited for live and ꞏ Must be very strongly Shows the specimens light on unstained samples with a low illuminated which may cause a dark background. reflection index or close to its damage to samples environment ꞏ Very useful for observing ꞏ...
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Page 6: Getting Results
Gain ꞏBrighter images with ꞏDimmer images with less Signal Intensity on the more noise noise detector Saturation ꞏImages will have more ꞏImages will have more Intensity of the color to purer colors washed up colors white Gamma ꞏHarder to differentiate ꞏHarder to differentiate Factor between linear and between brighter colors... -
Page 7: Taking Pictures
After figuring out the best field and camera parameters for your live view, taking and annotating pictures is the next step for making your work more discernible to others. The Leica Application Suite has basic tools that can be useful for the analysis of the microstructures within your sample. -
Page 8: Editing Pictures
Editing Pictures 1) Within the Process tab, select the Enhance window. 2) From here, you can change the following parameters of the photo: Brightness, Contrast, Gamma, Hue, Saturation, Intensity, Color, Black/White Levels. a. For the majority of the variables, they are now represented as 1’s or 0’s to display where the original image was before correction. 3) This tab also allows for physical changes to the photo through: a. Orientation: Allows for flipping along the X‐Y axis or a degree based rotation Analyzing Pictures 1) At the top of the screen, select the Interactive window within the Analysis tab 2) Here, you will see an assortment of tools as listed: ... -
Page 9: Finishing Up
Count Tool: Figure out how many of something there is on the sample Parallel Line Tool: Create parallel lines on your sample Cross Tool: Creates a cross on your sample Shape Tool: Creates a rectangle or a triangle on your sample. Note: Right clicking on the tool icon will reveal more options for data‐gathering. Such as showing either the diameter of a circle or its area= 3) Properties allows for the parameters of the tool annotations. They function very similarly to the settings within Annotating Pictures with a couple of additions. a. Display Labels: Allows for the displaying of elements such as units or numbers b. Label Offset: Determines how far away you want the label from the mark 4) Within the Automatic and Grain Expert windows, you can follow the computer‐ prompted steps to automatically analyze your picture and generate a report based on your preferences for processing. 5) When completed setting up the operation, press on Run Measurement to begin the computer‐analysis and report generation. Example: Simple Automatic Processing in Phase Measurement 1) Select your Image to Measure a. In the menu, choose your photo then press Append 2) Adjust your threshold a. Using the color wheel, adjust the hue and the saturation of the colors until your phases are properly selected. Clicking and dragging on the curve adjusts the hue while clicking and moving on the curve adjusts the saturation. 3) Click on Run Measurement to get information on the phase distribution in your image. ... -
Page 10: Before Leaving
Before Leaving 1) Reset all objective magnifications to their lowest value. 2) Make sure that all equipment is turned off a. Power off Sequence: Optical Microscope -> Computer 3) Clean up any and all messes created by the microscope to ensure a neat environment. Tips and Tricks Have curves in your path that you want to analyze? Hold the LMB on the mouse and move for a continuous path to see your total distance.
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