Differential Interference Contrast (Dic) - Leica DM IRE2 Manual

Differential interference contrast (DIC)

For DIC, you need:
 A DIC objective (i.e. any of the immersion
objectives)
 A polarizer above the condenser (no need to
insert this – it is kept in the light path at all times)
 A condenser prism in the condenser turret
 An analyzer beneath the objective prism turret
 An objective prism in the objective prism turret
To set up DIC:
 Select a DIC objective (any of the immersion
objectives)
 The objective DIC prism required for that objective
is displayed on the microscope control panel
 Manually rotate condenser turret to select the
appropriate objective DIC prism
Objective
100x oil
63x oil
63x water
40x
20x multi-immersion
 Manually rotate objective prism turret to select
objective prism
 Insert analyzer into light path
Condenser
prism
63/100
63/100
40/63
40/63
20
21
Objective
prism
D
E
D
E
C
CMN Imaging Core
January 16, 2013