Lx 400 User Manual Research Microscopy To ensure proper use of this instrument as well as to avoid injury while operating instrument, understanding this manual completely before use is highly recommended.
INTRODUCTION The Lx 400 is a research microscope reflecting a modern design as well as the latest in optical and mechanical advancements. Designed for professionals as well as clinicians, this microscope offers many features and functions for a diverse set of applications. Here are a few points highlighting the benefits of the Lx400: - Extra clarity and contrast is provided through a 360°...
Do not place microscope on a flexible surface, as this could result in blocking the air vents and cause overheating. 4. Always use the power cord provided by LABOMED. If the proper power cord is not used, product safety performance cannot be warranted.
Caution If the microscope is used in a manner not specified by this manual, the safety of the user may not be warranted. In addition, the equipment may also suffer damage. Always use the equipments as outlined in this instruction manual. Getting Started 1.
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Lx 400 Care & Maintenance Your microscope has been engineered for a long and safe operational life with the least amount of maintenance required. In general, routine maintenance is limited to keeping the microscopes working parts lubricated and optics clean. Always cover the microscope with the provided dust cover when not in use.
Lx 400 STANDARD COMPONENTS After removing your microscope from its packaging, make sure that all of the following contents are present. “Please note that the contents of your microscope may vary as the optional configuration, contrasting method or viewing body opted for may not be of the standard configuration highlighted here”...
OPTIONAL ACCESSORIES System Diagram of Optional Accessories Video adapter DSC adapter iVu 1500 iVu 3000 iVu 5000 iVu 7000 Binocular head Trinocular head WF 10x WF 16x WF 20x RP 4x RP 10x RP 20x RP 40x (SL) RP 50x (SL, Oil) RP 100x (SL, Oil) RP PH 10x RP PH 20x RP PH 40x (SL) RP PH 100x (SL, Oil) RP FL 4x...
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Lx 400 Optional Eyepieces 10X eyepieces are provided. To replace: 1. Pull out the 10x eyepieces out from the observation heads ocular tube. 2. Insert desired eyepieces in empty ocular tube. Fig. 5 Phase Turret 1. Remove the condenser from its position by loosening the thumb screw (1) on right side of the condenser bracket as shown in figure 6.
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Lx 400 4. Open the illumination assembly as in (figure 9), by rotating the T Ring. Rotate anti clockwise Replace the Lens B with frosted lens provided with the Koehler kit. T Ring Lens B Secure the lense assembly back.
INITIAL SETUP Objectives Objectives are factory set. Objectives are par-centered and par- focalised during assembly. All objectives have been secured for a tight fit to prevent them from coming loose during transit. To remove an objective, rotate it counterclockwise while holding it with a rubber grip to avoid any slippage.
Always use the designated bulb. Using a bulb other than those specified by LABOMED may lead to a fire hazard. Fingerprints or Fig. 15 stains on the lamp bulb reduce its life. If contamination occurs, wipe bulb surface with a cloth slightly moistened with alcohol.
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3. Replace the primary fuse (2) with the spare fuse. 4. Engage the fuse tray back in. Always use the designated Fuse. Using a fuse other than those specified by LABOMED may lead to a fire hazard. Fig. 19 Caution: For Fuse replacement Set the main switch to"...
SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE Flip the main switch to “ON” Place the specimen on the stage. Fuse Holder Main switch Engage the 10X objective in the light path. Bring the specimen in focus. Adjust the observation tube and eyepieces Adjust the interpupillary distance.
Lx 400 DETAILED OBSERVATION PROCEDURE Turning the Lamp ON 1. Flip the main switch to I (ON) ” ” as shown in Figure 19. 2. Rotating the light intensity adjustment knob (Fig. 20) in the direction of the arrow increases brightness and rotating knob in the opposite direction decreases brightness.
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Cover glass Cover glass This is the glass plate placed on the specimen. For optimum optical performance, the cover glass thickness, which is the distance from its surface to the specimen surface, should be 0.17 mm. Slide glass Slide glass This glass plate should ideally have a length of 76 mm, width of 26 mm ±1 mm and thickness between 0.9 and 1.4 mm.
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Lx 400 Adjusting the Interpupillary Distance (IPD) The inter-pupillary distance adjustment consists of regulating the two eyepieces to align with both eyes’ pupils so that you can observe a single microscopic image through two eyepieces in stereo vision. This greatly helps to reduce fatigue and discomfort during observation.
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Centering the Condenser The condenser is fixed and cannot de-center. In case of any error, remove the condenser by loosening thumb screw and re-install the same. For models with Koehler illumination: The Koehler mount has the floating diaphragm mount (fig. 30, 3). With this the Koehler can be centered wrt to the condenser.
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The designated immersion oil should be in contact with the cover lens of the 100X immersion objective. If not, the specimen will appear distorted and dull. It is recommended that LABOMED immersion oil is always used. Immersion Process: 1. Bring the specimen in focus using first the 10x, then 40x objective.
Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take corrective action as needed. If problem persists, please contact LABOMED or your local LABOMED dealer.
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Lx 400 Observation Cause Remedy 8. Objective hits the specimen when The specimen slide is upside down Set the specimen correctly with the an objective is switched to a higher cover glass facing upwards magnification objective The cover glass is too thick Use a cover glass with thickness of 0.17mm...
SPECIFICATIONS 1. Illumination Built-in illumination system Halogen/ LED 2. Focusing mechanism Stage height adjustment mechanism Fine adjustment scale: 3.0µm per graduation Fine adjustment stroke: 0.3mm per turn Total stroke: 12.7mm Co-axial coarse and fine focusing on ball drive 3. Revolving nosepiece Quadruple positions fixed (Reverse angle) 4.
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