Labomed TCM 400 User Manual

Labomed TCM 400 User Manual

Inverted tissue culture microscopy
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TCM 400
Inverted Tissue Culture Microscopy
User Manual
To ensure proper use of this instrument as well as to avoid injury while operating
Instrument, understanding this manual completely before use is highly recommended.
Part No: 7125000-795
Issue 1.3
Printed on April, 2022

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Summary of Contents for Labomed TCM 400

  • Page 1 TCM 400 Inverted Tissue Culture Microscopy User Manual To ensure proper use of this instrument as well as to avoid injury while operating Part No: 7125000-795 Instrument, understanding this manual completely before use is highly recommended. Issue 1.3 Printed on April, 2022...
  • Page 3: Table Of Contents

    TCM 400 TABLE OF CONTENTS 1. INTRODUCTION 2. SAFETY INFORMATION 3. TCM 400 BINOCULAR 4. TCM 400 TRINOCULAR 5. TCM 400 ERGONOMIC 6. UNPACKING YOUR MICROSCOPE 7. STANDARD COMPONENTS 8. OPTIONAL ACCESSORIES 10-11 9. INITIAL SETUP & ASSEMBLY 12-16 10. SUMMARY OF BRIGHT FIELD OBSERVATION PROCEDURE 11.
  • Page 4: Introduction

    It combines high performance and distinctive styling with the high quality craftmanship for which Labom- ed is known. The excellent image and comfort of use are the basics of Labomed TCM 400. The ergonomics of the design, an aesthetically configured TCM400 is the instant choice for Culture studies in the era of bio sciences.
  • Page 5: Safety Information

    Do not place microscope on a flexible surface, as this could result in blocking the air vents and cause overheating. 4. Always use the power cord provided by LABOMED. If the proper power cord is not used, product safety performance cannot be warranted.
  • Page 6 TCM 400 This icon represents the power switch is turned OFF. This icon represents the power switch is turned ON. Labeling Information Number following this symbol indicates the serial number of the equipment. Character following the symbol indicate the catalog number of the equipment.
  • Page 7 TCM 400 CARE & MAINTENANCE Your microscope had been engineered for a long and safe operational life with the least amount of mainte- nance required. In general, routine maintenance is limited to keeping the microscope working parts lubri- cated and optics clean. Always cover the microscope with the provided dust cover when not in use.
  • Page 8: Tcm 400 Binocular

    TCM 400 TCM 400 BINOCULAR Lamp Housing Illumination housing Binocular head Stage plate Specimen holder Phase contrast slider Eyepieces Microscope body Illumination intensity control knob Revolving Nose piece Main Switch Coarse and fine focus knob Issue 1.3 7125000-795 TCM 400...
  • Page 9: Tcm 400 Trinocular

    TCM 400 TCM 400 TRINOCULAR Lamp Housing Trinocular Port Illumination housing Stage plate Specimen holder Phase contrast slider Eyepieces Microscope body Illumination intensity control knob Main Switch Revolving Nose piece Coarse and fine focus knob Issue 1.3 7125000-795 TCM 400...
  • Page 10: Tcm 400 Ergonomic

    TCM 400 TCM 400 ERGONOMIC Lamp Housing Ergonomic head Illumination housing Stage plate Specimen holder Eyepieces Phase contrast slider Microscope body Illumination intensity control knob Main Switch Revolving Nose piece Coarse and fine focus knob Issue 1.3 7125000-795 TCM 400...
  • Page 11: Unpacking Your Microscope

    TCM 400 UNPACKING YOUR MICROSCOPE TCM400 comes securely packed in the custom configured foam packing, to prevent it from all shocks and transit damages. make sure that you receive all the following components before discarding packing material: a. Microscope body b.
  • Page 12: Standard Components

    TCM 400 STANDARD COMPONENTS After removing your microscope from its packaging, make sure that all of the following contents are present. “Please note that the contents of your microscope may vary as the optional configuration, contrasting method or viewing body opted for may not be of the standard configuration highlighted here”.
  • Page 13: Optional Accessories

    TCM 400 OPTIONAL ACCESSORIES SYSTEM DIAGRAM OF OPTIONAL ACCESSORIES CMB CAMERA ADAPTER ATLAS CMOS CAMERA iVU TOUCH SCREEN VEGA CMOS CAMERA Binocular head Trinocular head Ergonomic head WF 16x WF 20x WF 10x LW PH 4x LW PH 10x LW PH 20x...
  • Page 14 TCM 400 OPTIONAL EYEPIECES 10X eyepieces are provided. To replace: 1. Pull out the 10x eyepieces out from the observation heads Ocular tube. 2. Insert desired eyepieces in empty ocular tube.(Fig.3.1) Fig. 3.1 Fig. 3.2 Issue 1.3 TCM 400 7125000-795...
  • Page 15: Initial Setup & Assembly

    TCM 400 INITIAL SETUP & ASSEMBLY MOUNTING THE OBJECTIVES Settle down the nosepieces by rotating coarse adjustment knob towards back. Remove the dust prevention caps from the nose pieces. Screw the objective with the lowest magnification into the revolv- ing nose-piece from left side of the microscope. Turn the nose piece clockwise and mount the remaining objectives in ascending magnification order.
  • Page 16 TCM 400 BASE UNIT Base unit is assembled with co-axial focus mechanism, turret, stage plate and the lamp house arm as shown in figure (fig 7) as shown. Fig. 7 INSTALLING THE TRANSMITTED LIGHT ILLUMINATOR 1. Insert the illuminator into the arm opening and rotate the illuminator 90°...
  • Page 17 TCM 400 PHASE SLIDER/ FILTER 1. Hold the phase slider face up (LABOMED written facing upward) with the finger on the right and slide it gently into the slot. As shown in Fig. 9. Phase slider has click stops at the three positions i.e. 4x, 10x/20x and bright field stop .
  • Page 18 TCM 400 ADJUSTING THE INTERPUPILLARY DISTANCE AND DIOPTER While looking through the eyepieces, move both eyepieces until the left and right fields of view coincide completely. This matches your IPD. Fig. 12 ADJUSTING THE DIOPTER Adjust the Diopter setting to “0” on the left side eyepiece.
  • Page 19 TCM 400 BULB REPLACEMENT Use only recommended 6V-30W high intensity Halogen bulb. Guide hole Pull open the Lamp socket and pull out the blown bulb using a soft cloth. Gently insert the new bulb. Do not squeeze or press the bulb too much which can cause damage to the bulb and hand.
  • Page 20: Summary Of Bright Field Observation Procedure

    TCM 400 SUMMARY OF BRIGHT FIELD OBSERVATION PROCEDURE Flip the main switch to “ON” Place the specimen on the stage. On/Off Engage the 10X objective in the light path. Bring the specimen in focus. Adjust the observation tube and eyepieces Adjust the interpupillary distance.
  • Page 21: Detailed Observation Procedure

    TCM 400 DETAILED OBSERVATION PROCEDURE TURNING THE LAMP ON 1. Flip the main switch to ”I” (ON) as shown in Figure 17. 2. Rotating the light intensity adjustment knob in the direction of the arrow increases brightness and rotating knob in the oppo- site direction decreases brightness.
  • Page 22 TCM 400 ADJUSTING THE INTERPUPILLARY DISTANCE (IPD) The inter-pupillary distance adjustment consists of regulating the two eyepieces to align with both eyes’ pupils so that you can observe a single microscopic image through two eyepieces in stereo vision. This greatly helps to reduce fatigue and discomfort during observation.
  • Page 23: Troubleshooting Guide

    Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take corrective action as needed. If problem Persists, please contact LABOMED or your local LABOMED dealer. OBSERVATION...
  • Page 24: Specifications

    TCM 400 TECHNICAL SPECIFICATIONS Built-in illumination system Halogen 1. Illumination 2. Focusing mechanism Stage height adjustment mechanism Fine adjustment scale: 0.002 per graduation Fine adjustment stroke: 0.2mm per turn Total stroke: 28mm Co-axial coarse and fine focusing on ball drive Coarse adjustment travel per rotation 3.
  • Page 25 Revision History Rev. No. Date of Release DCR # Change App. By April 14, 2022 DCR/06/22 Reviewed & Up S.Bal dated for typo error...
  • Page 26 Labo America Inc. 920 Auburn Court Fremont, CA 94538 U.S.A. Phone: 510-445-1257 Fax: 510-991-9862 Email: sales@laboamerica.com www.laboamerica.com EU REP. Labomed Europe Essebaan 50 NL-2908 LK Capelle a/d IJssel The Netherlands Tel: +31 (0)10 4584222 Fax: +31 (0)10 4508251 E-mail: info@labomedeurope.com...

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