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Related Manuals for Knauer Azura FC 6.1
Summary of Contents for Knauer Azura FC 6.1
- Page 1 Fraction Collector FC 6.1 Instructions HPLC HPLC Document no. V6777...
- Page 2 KNAUER. Support: Do you have questions about the installation or the operation of your instrument or software? International Support: Contact your local KNAUER partner for support: www.knauer.net/en/Support/Distributors-worldwide Support in Germany (Austria & Switzerland on case-to-case basis): Phone:...
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Page 3: Table Of Contents
Table of contents General ................1 About these instructions . - Page 4 Table of contents Meaning of the LEDs ............11 Symbols and signs .
- Page 5 Table of contents Functionality tests ..............32 Installation Qualification (IQ) .
- Page 6 Table of contents 13. Chemical compatibility of wetted materials ..........51 13.1 General .
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Page 7: General
You can download these and other instructions from the KNAUER web- site: www.knauer.net/library. Warnings Possible dangers related to the device are distinguished in personal and material damages. -
Page 8: Transport Damage
1.4.3 Warranty conditions For information on warranty refer to our general terms and conditions on the website: www.knauer.net/terms 1.4.4 Declaration of conformity The declaration of conformity is enclosed as a separate document with the product and can be obtained online: www.knauer.net/de/Support/... -
Page 9: Basic Safety Instructions
ƒ hol or medication. They have participated in the installation of an instrument or training ƒ by KNAUER or an authorized company. If users do not meet these qualifications, they have to inform their supervisors. Operator responsibility The operator is any person who operates the device himself or leaves it to a third party for use. -
Page 10: Personal Safety Equipment
Basic safety instructions The obligations of the operator are listed below: Know and follow the applicable work safety regulations. ƒ Identify hazards arising from the working conditions at the place of use ƒ in a risk assessment. Set up operating instructions for the operation of the device. ƒ... -
Page 11: Contamination By Health-Threatening Solvents
Basic safety instructions Mobile phases and samples may contain volatile or combustible sol- ƒ vents. Avoid the accumulation of these substances. Ensure good ven- tilation of the installation site. Avoid open flames and sparks. Do not operate the instrument in the presence of flammable gases or vapors. Only use solvents which do not self-ignite under given conditions. -
Page 12: Specific Environments
ƒ system in order to avoid dead volumes, not exactly fitting connections and spreading contamination. Safety features: The device may only be opened by the KNAUER Cus- ƒ tomer Support or any company authorized by KNAUER (see chapter 1.4.1 on page 1). -
Page 13: Service Request Form And Decontamination Report
Devices which are shipped without the completed document “Service request form and decontamination report” will not be repaired. If you would like to return a device to KNAUER, make sure to enclose the com- pleted document: www.knauer.net/servicerequest. AZURA® Fraction Collector FC 6.1, Instructions, V6777... -
Page 14: Product Information
Service interface ƒ Ports for the LAN connection. ƒ The fraction collector is integrated into the KNAUER software environ- ment and is supported by PurityChrom, ClarityChrom and MobileControl. For efficient HPLC / UHPLC separations, observe the following instructions: Use highly purified, filtered solvents (gradient grade). -
Page 15: Device Variants
Product information Device variants The fraction collector is suitable for bio- and reversed-phase chromatog- raphy. Operation is possible for 1/16" and 1/8" via 1/4-28" UNF flat-bot- tom thread. However, the focus is on performance when using 1/16" capillaries and low flow (1-50 ml/min). Scope of delivery The following items are included in the scope of delivery: Fraction collector FC 6.1... -
Page 16: Rear View
Product information 3.4.2 Rear view In addition to the serial number and symbols (see chapter 3.6 on page 12), you also find the LAN ports, the connection for the power supply, the service interface and event connection on the back of the device. Note: The service interface is only used for maintenance and repair work by a service technician. -
Page 17: Meaning Of The Leds
Product information Meaning of the LEDs There is an LED in the on/off switch on the top and an LED on the device’s drop former. The illustration shows the LED display when the device is switched on. Fig. 4: LED on the top of the device Fig. -
Page 18: Symbols And Signs
Product information Color Operating status What to do Wait until the device is ƒ Flashes Initialization or re- Only LED on the drop ready. green home, firmware is former being updated (device is not yet ready for use). Green The device is switched (permanent) on. - Page 19 Product information Symbol Meaning The electromagnetic interference from the device is below the limits approved by the Federal Communications Commission (FCC). The device complies with the Australian EMV regulations. The device complies with Industry Canada CAN ICES-3 (B) license-exempt RSS standards. AZURA® Fraction Collector FC 6.1, Instructions, V6777...
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Page 20: Installation And Initial Startup
4. Installation and initial startup Before determining the operation site, read the chapter Technical data (see chapter 11 on page 47). There you find all device-specific informa- tion on the power supply and the permissible ambient conditions. Note: The intended use be ensured only if the requirements for ambient conditions of the operating environment are met. -
Page 21: Power Supply
Electronic defect Electronic hazard when using an identically constructed power adapter from another manufacturer. Only use spare parts and accessories from KNAUER or a company authorized by KNAUER. Country-specific plugs: Before switching on the device, check wheth- ƒ... -
Page 22: Unpacking
Installation and initial startup Unpacking Process Procedure 1. Place the packaging in such a way that the lettering on the label is in the correct position. 2. Check the packaging, the device and the accessories for transport damage. 3. Check the scope of delivery. In the event of an incomplete delivery, contact Technical Customer Service immediately. -
Page 23: Installing
Installation and initial startup Legend Holding rods Holding nuts Washers Screws Fig. 8: Holding rods Installing Prerequisite The individual components of the device were unpacked. ƒ Tools Allen wrench Torx 30 ƒ Allen wrench 1.5 mm ƒ Process Figures Procedure 1. - Page 24 Installation and initial startup Process Figures 5. Set the desired height and fix the motor block to the holding rods by tightening the knurled nut on the right-hand side of the clamping block. Note: Adjust the height of the fraction collector so that the swivel arm does not touch the vessels inserted in the rack and the liquid does not splash when arriving in the...
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Page 25: Integrating The Device Into An Hplc System
Installation and initial startup Integrating the device into an HPLC system Position Set up the device to the side of an HPLC system or place it on the top of the housing of an AZURA-L device. The device’s feet fit into the recesses on the top of the housing. -
Page 26: Connecting Capillaries To Magnetic Valve
Installation and initial startup Connecting capillaries to magnetic valve The magnetic valve on the swivel arm of the fraction collector has three connectors for liquids. The inlet of the magnetic valve is the horizontal connection (running parallel to the swivel arm). It is used to connect the capillary that comes from the LC system and conveys the liquid. -
Page 27: Connecting The Leakage Management System
Installation and initial startup Connecting the leakage management system The leakage management system consists of a drain with a drain nozzle on the underside of the ground plate and a silicone hose (OD 12 mm). The drainage system ensures that leaked liquids automatically flow into a waste bottle. -
Page 28: Computer Control
Installation and initial startup Computer control Note: HPLC devices from KNAUER only work with IP addresses, which have been assigned by IPv4. IPv6 is not supported. This chapter describes how to set up a chromatography system in a local area network (LAN) and how a network administrator can integrate this LAN into your company network. -
Page 29: Configuring Lan Settings
Installation and initial startup 4.8.2 Configuring LAN settings The LAN uses only one server (which is normally the router) from that the devices automatically receive their IP address. Prerequisites In Windows, power saving, hibernation, standby, and screen saver has ƒ to be deactivated. -
Page 30: Configuring The Router
Set the router properties (see section 4.8.4). 4.8.4 Configuring the router The router is preset at the factory. Information about address, user name and password is noted in the router manual: www.knauer.net/en/ Support/Library/PC-hardware. Process Procedure 1. To open the router configuration, start your Internet browser and enter the IP address (does not apply for all routers). -
Page 31: Controlling Several Systems Separately In Lan
Installation and initial startup 4.8.6 Controlling several systems separately in LAN Devices connected to a LAN communicate through ports, which are part of the IP address. If more than one chromatography system is connected to the same LAN and you plan on controlling them separately, you can use different ports to avoid interference. -
Page 32: Mobile Control: Setting A Dynamic Ip Address Via Device Name
Installation and initial startup Process 5. If necessary, change the subnet mask and the gateway 6. Click in the top right corner. 7. Restart the device (recommended). Legend IP address mode Text box for IP address Text field for subnet mask/gateway Fig. -
Page 33: Mobile Control: Setting A Dynamic Ip Address Via Device Serial Number
Installation and initial startup Legend IP address mode Fig. 17: Network settings for dynamic IP address Result The device is now accessible via a dynamic IP address. 4.9.3 Mobile Control: Setting a dynamic IP address via device serial number Prerequisites The device is switched on. -
Page 34: Firmware Wizard: Setting A Static Ip Address
Installation and initial startup 4.9.4 Firmware Wizard: Setting a static IP address Note: More information about LAN settings can be found in the Mobile Control Software Instructions in the chapter "Firmware Wizard” (docu- ment no. V6851-4). Legend Text box for the device‘s serial number Setting IP address manually... -
Page 35: Firmware Wizard: Setting A Dynamic Ip Address
Installation and initial startup 4.9.5 Firmware Wizard: Setting a dynamic IP address Prerequisites The device is switched on. ƒ Firmware Wizard is installed and running. ƒ Process Procedure 1. In Firmware Wizard, click <Reset LAN Settings...>. The window <Device connection settings> opens. 2. -
Page 36: Connecting The Pin Header
Installation and initial startup 4.10.2 Connecting the pin header To control one device through another, you use the pin header. To use remote control, you have to connect cables to the pin header. The single ports are used to exchange control signals. Prerequisites The device is switched off. -
Page 37: Operation
ƒ If the device is part of an HPLC system, note the following: The network connection to the router is established. ƒ The chromatography software has been installed by KNAUER or a ƒ company authorized by KNAUER. Switching on After switching on the fraction collector it moves once through all angular positions. -
Page 38: Functionality Tests
Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge. In case of a request, the technical support of KNAUER or a pro- vider authorized by KNAUER performs this functionality test during the installation. -
Page 39: Troubleshooting
7. Troubleshooting First measures for troubleshooting: Check all cables and fittings. ƒ Check if air has got into the supply lines. ƒ Check the device for leakages. ƒ Further measures: Compare occurring errors with the list of possible errors (see below). ƒ... -
Page 40: Possible Problems And Solutions
Troubleshooting Possible problems and solutions Problem Solution Device cannot be Check the power cable to ensure that it is switched on. connected to the mains. System breakdown Switch off the device to reset the memory in the device, then switch it on again. Device shows error Switch the device off and on. -
Page 41: Error Messages
Troubleshooting Error messages If other error messages are displayed besides those listed below, restart the device. Inform the Technical Support of the manufacturer in case the error message displays repeatedly. The system messages are sorted by error code: Error Error name System message Correcting the error code... - Page 42 Troubleshooting Error Error name System message Correcting the error code No space Programming custom rack ex- The available device memory left to store hausts available device memory. is exhausted. Simplify your rack position No more entries can be added. definition. Simplify your custom rack layout.
- Page 43 Troubleshooting Error Error name System message Correcting the error code Not allowed on The command cannot be executed The software does not have write this interface on this communication interface. access to the device. Currently Looks like device already has only possible as a display.
- Page 44 Troubleshooting Error Error name System message Correcting the error code 100001 Rack Position X Rack position provided with a Requested position does not exist. is not valid command is not valid. Check the Check the position. command parameter(s). 100002 Rack id X is not No rack with this id is found in the Check the list of configured racks in the device...
- Page 45 Troubleshooting Error Error name System message Correcting the error code 100010 Actual span Actual span of polar reposition is Check the fraction collector for X.X° is too found too narrow during rehom- mechanical obstructions. Carry out narrow. Please ing. Some obstacles may reduce a new rehome.
- Page 46 Troubleshooting Error Error name System message Correcting the error code 100022 Position is out The device rotating position was Restart the device. If the error of drive control changed manually by the user. occurs again, inform the Technical by X°(Y µstep). Polar encoder synchronization was Support of the manufacturer.
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Page 47: Maintenance And Care
Switch off the device. Pull the power plug. Maintenance contract The device may only be opened by the Technical Service of KNAUER or any company authorized by KNAUER. These maintenance tasks are part of a separate maintenance contract. What maintenance work may users... -
Page 48: Cleaning And Caring For The Device
Maintenance and care Cleaning and caring for the device Device defect Intruding liquids can cause damage to the device. Place solvent bottles next to the device or in a solvent tray. Moisten the cleaning cloth only slightly. Clean all smooth surfaces of the device using a mild, commercially avail- able cleaning solution, or with isopropanol. -
Page 49: Transport And Storage
Transporting the device Documents: If you wish to return your device to KNAUER for repairs, ƒ enclose the document “Service request form and decontamination report”... -
Page 50: Storing The Device
Transport and storage Storing the device Solvent residues Solvent residues can damage the fraction collector. The resulting deposits can block the flow when using the device again. Flush the fraction collector before storage. Flush the valve with isopropanol. ... -
Page 51: Disposal
10.1 AVV-Marking Germany According to the German “Abfallverzeichnisverordnung” (AVV) (January, 2001), old devices manufactured by KNAUER are marked as waste electri- cal and electronic equipment: 160214. 10.2 WEEE registration number All WEEE registrations of KNAUER and the corresponding categories can be viewed on our website: www.knauer.net/imprint... -
Page 52: Technical Data
11. Technical data 11.1 Main features Faction modes Drop counting ƒ Time intervals ƒ Volume intervals ƒ Filling level ƒ Maximum flow rates Best working up to 100 ml/min; maximum 250 ml/min Fraction capacity Refer to the list of racks in the "Accessories and spare parts"... -
Page 53: Communication
Technical data 11.2 Communication Interfaces ƒ 3-way pin header plug ƒ (Analog IN, OUT, GROUND) Control ƒ Analog and event control ƒ Software ƒ Analog input TTL (0 or 5 V) Analog control input Change to the next position Injector valve cycles, radial drive revolutions, polar drive revolutions Display 2 LEDs... -
Page 54: Repeat Orders
Use the enclosed packing list for reordering spare parts. If you have any questions regarding spare parts or accessories, contact our Customer Support. More information Further information on spare parts and accessories can be found online: www.knauer.net. 12.1 Device Name Order no. AZURA® FC 6.1 BIO Fraction collector AFA00 AZURA®... -
Page 55: Spare Parts
Repeat orders 12.3 Spare parts Name Order no. Device feet Spare part kit device feet, self-adhesive, for L devices A8880028 Mainboard Spare part kit mainboard, complete A8880103 (including mainboard and two spacers) CR2032 Lithium battery 3V / 225 mAh, 3 pcs. A80171 Light barrier circuit board A80427... -
Page 56: Wear Parts
Repeat orders 12.4 Wear parts Name Order no. Maintenance kit Maintenance kit (for magnetic valve block) including ARF01 magnetic valve block, super flangeless ferrule, drop former capillary, fitting and protective tube Valve block Magnetic valve block A80439 Ferrule Super flangeless ferrule (10 ferrules for 1/16”, PEEK, A58292 1/4-28 Flat-Bottom, with lock ring SST, max. -
Page 57: Chemical Compatibility Of Wetted Materials
The following list contains information about the chemical compatibility of all wetted materials which are used in devices made by KNAUER. The data bases on a literature research on the manufacturer specifications of the materials. The wetted materials of this device are listed in the chapter “Technical data”. - Page 58 Chemical compatibility of wetted materials Polyimide (Vespel®) This material is wear-resistant and permanent resilient thermically (up to 200 °C) as well as mechanically. It is chemically broadly inert (pH range 1-10) and is especially resistant against acidic to neutral and organic solvents, but vulnerable to pH strong chemical or oxidizing environments: It is incompatible with concentrated mineral acids (such as sulfuric acid), glacial acetic acid, DMSO and THF.
- Page 59 Chemical compatibility of wetted materials Polychlortrifluorethylene (PCTFE, Kel-F®) The semi-crystalline thermoplastic material is plasticizer-free and dimen- sionally stable, even in a wide temperature range (–240 °C to+205 °C). It is moderately resistant against ether, halogenated solvents and toluene. Halogenated solvents over +60 °C and chlorine gas should not be used. Fluorinated rubber (FKM) The elastomer consisting of fluorinated hydrocarbon stands out due to a high resistance against mineral oils, synthetic hydraulic fluids, fuels,...
- Page 60 Chemical compatibility of wetted materials Ceramic Ceramic is resistant against corrosion and wear and is fully biocompati- ble. An incompatibility against acids, alkalis and solvents commonly used in HPLC is not known. Alumina (Al Due to their high resistance to wear and corrosion, alumina ceramic is used as a coating for mechanically stressed surfaces.
- Page 61 Customer approval Prior to installation at the customer site, the customer has reviewed the OQ document and agrees with the design and scope. Company name: Name Function Reviewed & approved Date Signature VIQ-Installation-Qualification-Device, Version 3.1 Date: Signature: Revision:...
- Page 62 KNAUER Wissenschaftliche Geräte GmbH. Scope The customer can request the Installation Qualification. In case of a request, the technical support of KNAUER or a provider authorized by KNAUER performs this functionality test during the installation. The IQ is a standardized document and includes the following: ƒ...
- Page 63 Installation Qualification Tests Test Description Specification Passed Failed Comment /LOR No. Identify the device. The name on the device matches the name on the delivery order. Check the device for No transport damage is transport damage. observed. Check the scope of The scope of delivery delivery.
- Page 64 List of rectifications (LOR) Type of Description of Persons LOR No. Test No. Action plan Due date Date/signature deviation* the deviation responsible * Type of deviation: A = acceptable (e.g. not a GMP-critical deviation) N = not acceptable Continuation of qualification activities into the next qualification phase is only possible when deviation is rectified. T = temporarily acceptable a) Release and use of the system is possible, even when the deviation is not rectified.
- Page 65 List of changes to the document Revision no. Description of change Additional information Date/signature VIQ-Installation-Qualification-Device, Version 3.1 Date: Signature: Revision:...
- Page 66 Installation Qualification (IQ) for a Device 10. Certificate and approval A KNAUER employee or an employee authorized by KNAUER has checked the device and performed all tests described in the IQ. The IQ form has to be signed by an authorized person. The scope of the IQ meets the customer‘s requirements.
- Page 67 Appendix: List of supporting documents Test no. Description VIQ-Installation-Qualification-Device, Version 3.1 Date: Signature: Revision:...
- Page 68 Latest KNAUER instructions online: www.knauer.net/library KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Fax: +49 30 8015010 Hegauer Weg 37-38 E-mail: info@knauer.net 14163 Berlin Internet: www.knauer.net © KNAUER 2025...
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