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BioLAB BAVT 400 B Series Operation Manual

Vertical autoclave
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Operation
Manual
BAVT 400 B series
Vertical Autoclave
Thank you for Choosing Biolab products. Please read the "Operating Instructions" and
"Warranty" before operating this unit to assure proper operation.

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Summary of Contents for BioLAB BAVT 400 B Series

  • Page 1 Operation Manual BAVT 400 B series Vertical Autoclave Thank you for Choosing Biolab products. Please read the “Operating Instructions” and “Warranty” before operating this unit to assure proper operation.
  • Page 2 Vertical Autoclave BAVT 400 B series Precaution on Safe Operation • To ensure the safe and correct use of the instrument, please read this manual carefully before use and operate according to the instructions in the manual. If it is not used according to the method specified by the manufacturer, the protection provided by the instrument may be damaged.
  • Page 3 Vertical Autoclave BAVT 400 B series a sufficiently low level to avoid scalding. • Distilled water must be used as sterilization water to avoid affecting the service life of sterilizer. When the instrument works continuously, it shall be ensured that there is an interval of more than 15 minutes for the instrument to cool down.
  • Page 4 Vertical Autoclave BAVT 400 B series Index Chapter 1 About the Instrument.............05 Chapter 2 Installation of Autoclave............12 Chapter 3 Operation Instruction.............13 Chapter 4 Maintain&Management............36 Chapter 5 Troubleshooting..............42 www.biolabscientific.com...

  • Page 5: About The Instrument

    Vertical Autoclave BAVT 400 B series About the instrument 1. Application • This series of products are used for sterilization of scientific research institutions, laboratory utensils, culture media and unsealed liquids or preparations. 2. Sterilization principle • Steam is used as sterilization factor to kill loaded microorganisms. The main technical parameters of sterilization, such as pressure, temperature and time, are set and controlled by the program.
  • Page 6 Vertical Autoclave BAVT 400 B series 9. Technical specifications Model BAVT-401-B BAVT- BAVT-403-B 402-B Capacity(L) 110L Dimension(L*W*H,mm) 644mmX831mmX980mm 644mmX 644mmX831m 831mmX mX1180mm 980mm Chamber φ400mm x505mm φ400mm φ400mmX895 dimension(Dia*H,mm) 700mm Net weight 130kg 135kg 146kg Rated power 2900W 4600W 4600W...
  • Page 7 Vertical Autoclave BAVT 400 B series Liquid：standby→heating→sterilizing→exhaust→end Liquid with warming： Sterilizing mode standby→heating→sterilizing→exhaust→warming→end Solid mode： Sandby→heating→sterilizing→exhaust→end Instruments packed with cloth： standby→heating→sterilzing→exhaus→end Fabric: standby→heating→sterilzing→exhaust →end Rubber: standby→heating→sterilzing→exhaust →end Fast: standby→heating→sterilizing→exhaust→end Waste：standby→heating →sterilizing →exhaust→end Agar： standby→heating→melting→exhaust→warming→end Self-defined： standby→heating→sterilizing→exhaust→warming→end Controller “Inspiration II”fast speed microcomputer controller...
  • Page 8 Vertical Autoclave BAVT 400 B series 10.Appearance and Parts www.biolabscientific.com...
  • Page 9 Vertical Autoclave BAVT 400 B series Parts Function Display Panel Function： 1.LOCKED: Interlocking indicator, which will be on during interlocking. 2.WATER: Water level indicator. Under standby condition, the indicator on means that there is sufficient water in sterilization chamber for sterilization, and indicator off indicates water shortage and then the system will automatically start for water intake.
  • Page 10 Vertical Autoclave BAVT 400 B series 3.LIQUID: The light indicates the current operation mode is liquid mode 4.SOLID: The light indicates the current operation mode is solid mode 5.AGAR: The light indicates the current operation mode is agar melting mode 6.CLOCK: The light indicates the current operation mode is auto startup mode...
  • Page 11 Vertical Autoclave BAVT 400 B series 27. WARM: *WARM* is light, and drying/warming status indicator is blinking to indicate that the instrument is in warming stage. If *WARM* and drying/warming status indicator blink together, it indicates entering the warming parameters modification status.
  • Page 12 Vertical Autoclave BAVT 400 B series 37.FAN: Start/Stop button for cooling fan is used to control the start and stop of fan from the completion of sterilization stage or drying/warming stage to the temp lower than 40*, while the cooling fan indicator will be on or off with this switching.
  • Page 13 Vertical Autoclave BAVT 400 B series Requirement for current intensity: BAVT-401-B ································ 20A BAVT-402-B································ 32A BAVT-403-B································ 32A 3）Connect the BAVT-402/403-B power cord to air switch with power pack ,of which, the red/brown wire connecting to live wire,green/blue wire to zero wire,yellow/green wire to earth wire.If it is installed with one plug/socket, connecct...
  • Page 14 Vertical Autoclave BAVT 400 B series Operation Instruction I Basic Sterilization Illustration &Operation Instruction Turn on the power Open the sterilizer chamber cover Add water into chamber Load articles to be sterilized Close the sterilizer chamber cover Choose or set the sterilizing...
  • Page 15 Vertical Autoclave BAVT 400 B series 1 Turn on the power ●Turn on the switch on the left side of the machine。 ： ST-BY” will ●After turn on the power, the system will do the self-checking “ blink，when the handle is at “locked” position, screen A will show current temperature, screen B will show newly saved program no.
  • Page 16 Vertical Autoclave BAVT 400 B series 3 Automatic Water Feeding ●Pour condensate water: place a 10L external water tank on the right side of the front of the instrument for the discharge after sterilization. Check that the water volume of the front water tank is below the high position. If it is half of the high position, please pour out.
  • Page 17 Vertical Autoclave BAVT 400 B series The water level inside the chamber may decrease after each cycle, please add new water on time.Lack of water for a long time may cause damage to the heater. It is recommended to use distilled water with an electricity conductivity between 10~15us/cm, do not use well water, salt water or hard water, so as to prevent the chamber from corrosion, fouling and shortening the life of heater.
  • Page 18 Vertical Autoclave BAVT 400 B series Close the sterilizer chamber cover ●Press the middle part of the front end of the chamber cover gently, push the cover opening / closing handle to the left to the locked position, the system will give a sound, and the indicator light "locked"...
  • Page 19 Vertical Autoclave BAVT 400 B series U01 default parameters and the parameters scope of its newly created program Name Default Parameter Parameters Range of New Program Sterilizing Temp. ① 121 ℃ 105℃~138℃ Sterilizing Time ② 20min 1~6000min Exhaust ③ 0~5level...
  • Page 20 Vertical Autoclave BAVT 400 B series U02 default parameters and the parameters scope of its newly created program Name Default Parameter Parameters Range of New Program Sterilizing Temp. ① 121℃ 105℃~138℃ Sterilizing Time ② 20min 1~6000min Warming Temp. ③ 50 ℃...
  • Page 21 Vertical Autoclave BAVT 400 B series parameters once by five screens: U03 default parameters and the parameters scope of its newly created program Name Default Parameter Parameters Range of New Program Sterilizing Temp. ① 121℃ 105℃~138℃ Sterilizing Time ② 20 min...
  • Page 22 Vertical Autoclave BAVT 400 B series U04 default parameters and the parameters scope of its newly created program Name Default Parameter Parameters Range of New Program Sterilizing Temp. ① 121℃ 105℃~138℃ Sterilizing Time ② 30min 1~6000min Exhaust ③ 0~5level Level(EHT) ④...
  • Page 23 Vertical Autoclave BAVT 400 B series U0 default parameters and the range of new program parameters generated by u05: 6）U06—Rubber ○Sterilizing flow：Standby→Heating→Sterilizing→Exhaust→Complete ○Application：Sterilization of heat-resistant and moisture-resistant tubular rubber, porous rubber products, etc. ○Press DATA button, the digital displayer A and B will display the detailed parameters once by 5 screens: www.biolabscientific.com...
  • Page 24 Vertical Autoclave BAVT 400 B series Name Default Parameter Parameters Range of New Program Sterilizing Temp ① 121 ℃ 105℃~138℃ Sterilizing Time ② 30min 1~6000min Exhaust ③ 0~5level Level(EHT) ④ Delay 0min 0~10min Time（DELY） ⑤ Lid Open Temp. 97℃ 40℃~99℃...
  • Page 25 Vertical Autoclave BAVT 400 B series Name Default Parameter Parameters Range of New Program Sterilizing Temp ① 134℃ 105℃~138℃ Sterilizing Time ② 12min 1~6000min Exhaust Level(EHT) ③ 0~5 level 0 min 0~10mmin Delay Time（DELY） ④ Lid Open Temp. ⑤ 97℃...
  • Page 26 Vertical Autoclave BAVT 400 B series Name Default Parameter Parameters Range of New Program Sterilizing Temp ① 126℃ 105℃~138℃ Sterilizing Time ② 40min 1~6000min Exhaust ③ 0level 0~5level Level(EHT) Delay ④ 0min 0~10min Time（DELY） Lid Open Temp. ⑤ 80℃ 40℃~99℃...
  • Page 27 Vertical Autoclave BAVT 400 B series Name Default Parameter Parameters Range of New Program Melting Temp. ① 100 ℃ 60℃~115℃ Melting Time ② 10min 1~6000min Warming Temp. ③ 50℃ 45℃~79℃ Warming Time ④ 600min 1~9999min Exhaust ⑤ 0~5level Level(EHT) Lid Open Temp.
  • Page 28 Vertical Autoclave BAVT 400 B series U10 default parameters and the parameters scope of its newly created program Name Default Parameters Note Parameter Range of New Program Sterilizing ① 121℃ 105℃~138℃ Temp. Sterilizing ② 20min 1~6000min Time Warming ③ 50℃...
  • Page 29 Vertical Autoclave BAVT 400 B series Time（DELY） Lid Open 97℃ 40℃~99℃ Temp.（OPEN) Expre ssion faulty ⑧ Hot air 0min 0~250min holding time（StAr） Note：To make sure safety, the lid can be open only when the temperature is lower than the set OPEN temperature. If the machine is added with load thermometer, the lid can be open only when both the chamber temperature and thermometer temperature are lower than the OPEN temperature.
  • Page 30 Vertical Autoclave BAVT 400 B series For details, please refer to the third part of the third chapter of the operating instructions, and for setting the timing start, see the fourth part of the third chapter 5) Program printing function: if the printer is installed and set in the administrator menu, enable it.
  • Page 31 Vertical Autoclave BAVT 400 B series sterilization chamber. II Creating, Modifying, Deleting the Program 1 Creating and modifying the program Find the required sterilization mode by pressing the "up" or "down" key, and then press the "set / ENT" key to enter the parameter modification interface. If there is a function of setting and enabling password in the background, the administrator password should be input.
  • Page 32 Vertical Autoclave BAVT 400 B series ideal sterilization effect, it is recommended to use the load thermometer; if no, set a longer sterilization time. For example, for 3L water in a flask, when the chamber temperature reaches set temperature after 20 minutes, but the liquid in the bottle hasn’t reach yet, it needs 33 minutes more to reach.
  • Page 33 Vertical Autoclave BAVT 400 B series can be modified. Press the "up" or "down" key to set. 3) Press the "next" key, and the time and month indicator lights on the digital display screen a will flash, indicating that it can be modified; press the "up" or "down"...
  • Page 34 Vertical Autoclave BAVT 400 B series and the display is 0 minutes. 7) When all parameters are modified, and then press "set / ENT" key, the modified parameters will be permanently saved, even if power failure will not be eliminated. If you do not want to save your changes, you can press the "data" key to return to standby mode.
  • Page 35 Vertical Autoclave BAVT 400 B series Startup Time ** Expression is 0-59 faulty ** 2. After setting the correct delay time, press DATA to exit. 3. If the delay time is not set correctly, press the "set / ENT" key to enter the parameter modification state: digital display B (delay days), flashing, indicating that it can be modified.
  • Page 36 Vertical Autoclave BAVT 400 B series Maintain and Management Note：Turn off the power, make sure the chamber is cooled down before maintain or fix. Cleaning 1 Water inlet tank cleaning ● Connect the drainage to the drain port, then open it counterclockwise.
  • Page 37 Vertical Autoclave BAVT 400 B series ●Clean the sterilization chamber with a soft cloth, and then wash it with hot water (do not add any detergent)。 ●Clean the chamber once a week. 3 Cleaning of drainage and inlet filters ●The drainage and water inlet filters are located at the back and bottom of the...
  • Page 38 Vertical Autoclave BAVT 400 B series ●Take out the water plate and check whether the surface Of heater is clean or not. Otherwise, wash with soft brush, And then empty the dirty water ●Do not move or damage the temperature control switch on the heater ●Suggest to clean once a month...
  • Page 39 Vertical Autoclave BAVT 400 B series 7 Maintain of sealing ring ●Change the sealing ring if there is any broken。If the edge of the sealing ring of cover becomes white or hardened, it may lead to steam leakage, and must be changed.
  • Page 40 Vertical Autoclave BAVT 400 B series upwards, take out the old sealing ring, and then take out the fixing ring of the sealing ring from the old sealing ring. ●Clean the dirt on the fixing ring of the sealing ring and the contact part between the chamber and the sealing ring with a cloth.
  • Page 41 Vertical Autoclave BAVT 400 B series accessory or pressure sensor is off P004 Display of printing date: 0: DDMMYY; 1: YYMMDD P005 Printing language: 0: English; 1: Chinese P006 Safety valve test: yes for enabled; no for disabled P007 Chamber temp sensor’s temp compensation: the temp compensation scope is -5--+5;...

  • Page 42: Troubleshooting

    Vertical Autoclave BAVT 400 B series Troubleshooting The automatic control system of the sterilizer monitors the real time operation of the instrument. Whenever any failure occurs, the system may send out alarm and display the error code, then please press STOP button to return standby status, and turn off the power, check the error code and handle on time.
  • Page 43 Vertical Autoclave BAVT 400 B series E-12 Micro pressure switch problem Contact local distributor to change micro pressure switch E-14 Drying system or temp. control Contact local distributor problem E-15 Low water in the chamber Add water into chamber E-16...
  • Page 44 Vertical Autoclave BAVT 400 B series The above table only covers the simple problem, if you can not deal with, please record the below informaiotn and report to the dealer: 1 ） Instrument model and serial No. 2) Error code 3) At what temperature it occurs, what articels sterilized, picture or videoif have 4 ）...
  • Page 45 BIOLAB SCIENTIFIC LTD. 3660 Midland Avenue, Suite 300, Toronto, Ontario M1V 0B8 Canada Email: contact@biolabscientific.com Tel: +1 707 533 1445 Website: www.biolabscientific.com...

This manual is also suitable for:

Bavt-401-bBavt-402-bBavt-403-b