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BioLAB BAHZ-401-A Operation Manual

Horizontal autoclave
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Operation
Manual
BAHZ-401-A & BAHZ-402-A
Horizontal Autoclave
Thank you for Choosing Biolab products. Please read the "Operating Instructions" and
"Warranty" before operating this unit to assure proper operation.

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Summary of Contents for BioLAB BAHZ-401-A

  • Page 1 Operation Manual BAHZ-401-A & BAHZ-402-A Horizontal Autoclave Thank you for Choosing Biolab products. Please read the “Operating Instructions” and “Warranty” before operating this unit to assure proper operation.

  • Page 2: Table Of Contents

    Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Index 1. Precaution on Safe Operation...................03 2. About the Instrument.....................05 3. Installation of Autoclave......................08 4. Maintain & Management.....................40 5. Troubleshooting........................45 6. Electrical Wiring Diagram and Component Distribution Diagram.....49 7. Pipeline Diagram........................52 www.biolabscientific.com...

  • Page 3: Precaution On Safe Operation

    Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Precaution on Safe Operation • To ensure the safe and correct use of the instrument, please read this manual carefully before use and operate according to the instructions in the manual. If it is not used according to the method specified by the manufacturer, the protection provided by the instrument may be damaged.
  • Page 4 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A sterilizer. When the instrument works continuously, it shall be ensured that there is an interval of more than 15 minutes for the instrument to cool down. Otherwise, the instrument will not be able to produce enough saturated steam.

  • Page 5: About The Instrument

    Horizontal Autoclave BAHZ-401-A & BAHZ-402-A About the Instrument 1. Application • This series of products are used for sterilization of scientific research institutions, laboratory utensils, culture media and unsealed liquids or preparations. 2. Types of microorganisms killed • Using hot and humid high pressure steam as sterilization factor to kill loaded microorganisms, including spores of bacteria, spores of fungi, etc.
  • Page 6 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Chamber φ508*1130 φ608*1130 dimension(Dia*H,mm) Net weight 650kg 720kg Total power 16kW Chamber material S30408 Sterilization temperature 115℃～135℃ Rated working pressure 0.25MPa Display range of -0.1～0.5MPa pressure gauge Set pressure of safety 0.29MPa valve Sterilization time range 1min～6000min...
  • Page 7 (optional), back pressure system Note 1: the material of sterilization chamber can be purchased s31603, and the model is equipped with - L, for example: BAHZ-401-A becomes BAHZ-401-A-l; 8. Introduction to the main structure of the product It is mainly composed of shell (shell decoration cover), sterilization chamber, sterilization chamber door, built-in steam generator, pipeline system, temperature control system, pressure detection, safety interlock device and pulsating vacuum pumping device.

  • Page 8: Installation Of Autoclave

    Horizontal Autoclave BAHZ-401-A & BAHZ-402-A control signals to automatically complete the control process; ●Built in steam generator unit: The electric heating generator is a device that can automatically generate steam, and has the functions of automatic control of water inlet, heating, dry burning prevention and overpressure protection;...
  • Page 9 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 1.Preparation before installation ●According to the installation drawing provided by our company, under the guidance of professionals, professional construction personnel are responsible for it; ●Location and environmental requirements: 1) According to the size of the instrument's installation drawing, reserve the installation space of the instrument to meet the convenience of instrument installation, maintenance and use;...
  • Page 10 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A combustible gas environment. 4) Avoid installation where electric shock or vibration may occur. 5) Avoid installation in places with high temperature and humidity or easy to be wet by rain. 6) Avoid installation in strong magnetic field.
  • Page 11 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A such as whether there are scratches on the surface of the panel, whether the knob is broken, etc. if you find any problems, please contact our company immediately. 3.Instrument installation ●After the instrument is hoisted or transported in place, adjust the height of the foot cup to make the instrument adjust to the horizontal position (based on the storage plate inside the container) and make the sealing door open and close freely.
  • Page 12 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Instrument debugging Some programs have been set in advance before the instrument leaves the factory. In the process of equipment debugging, users (technicians) can set the sterilization parameters according to the use of sterilization materials.
  • Page 13 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Turn off the power It is strictly forbidden for non operators to touch the control screen at will 1.Turn on the power supply and water source switch Turn on the water source and main power switch of the equipment (first close the small blue handle, then the 4P handle), and then turn on the key switch and emergency stop switch on the left side of the pressure gauge.
  • Page 14 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Note: When the machine leaves the factory, the administrator number is set as 15; the password of the administrator is 1234567; the password of the operator (No. 1-10) and the technician (No. 11-14) is set by the administrator, and the user without password is the user who is not enabled.
  • Page 15 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Press OK to return to the operation login interface. 2.3. Click "open door" to display the following screen: After the interlock is opened, the following interface appears: www.biolabscientific.com...
  • Page 16 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A When the inner chamber pressure returns to atmospheric pressure, the chamber door will automatically pop open, indicating the following interface: Note: If the system can not automatically restore to atmospheric pressure, please click "negative pressure release" to manually restore.
  • Page 17 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Preparation before sterilization of rubber tube: Please clean the rubber tube with warm water first, and then place it on the sterilizer storage board. At the same time, make sure that the pipe is a hollow pipe with open ends, and there is no sharp turning, twisting or kinking.
  • Page 18 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A After the prompt disappears, the following screen appears: When the door lock is locked, the prompt will disappear after a few seconds. (Note: the dooroperation cannot be performed when the sterilization program is started) 5.Select program Click "program"...
  • Page 19 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Sterilization purpose: fabric sterilization, drying after sterilization Click the fabric program to pop up the following screen: Parameter setting range: Name Default parameters Program parameter range Sterilizing Temp. 134℃ 115℃～135℃ Sterilizing Time 4min 1min～6000min Sterilization pressure...
  • Page 20 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Note: 1) exhaust speed setting Description: stage 0 means exhaust closing, stage 1, 2, 3 and 4 exhaust time is lengthened in turn, and stage 5 is exhaust normally open. 2) When the administrator sets "print setting" to be controlled by "operator", the operator can start and stop printing 5.2 Device program:...
  • Page 21 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Note: 1) exhaust speed setting Description: stage 0 means exhaust closing, stage 1, 2, 3 and 4 exhaust time is lengthened in turn, and stage 5 is exhaust normally open. 3) When the administrator sets "print setting" to be controlled by "operator", the operator can start and stop printing 5.3 Liquid procedure:...
  • Page 22 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Note: 1) exhaust speed setting Description: stage 0 means exhaust closing, stage 1, 2, 3 and 4 exhaust time is lengthened in turn, and stage 5 is exhaust normally open. 2) When the administrator sets "print setting" to be controlled by "operator", the operator can start and stop printing 5.4 BD Program...
  • Page 23 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Note: 1) description of exhaust speed setting: level 0 means no exhaust, stage 1, 2, 3 and 4 exhaust speed increases in turn, and level 5 means exhaust normally open. 2) When the administrator sets "print setting" to be controlled by "operator", the operator can start and stop printing 5.5 vacuum test program...
  • Page 24 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Parameter setting range: Name Default parameters Program parameter range Sterilizing Temp. 134℃ 115℃～135℃ Sterilizing Time 4min 1min～6000min Sterilization pressure It changes automatically with the No modification is setting change of sterilization recommended temperature Chamber cover opening 99℃...
  • Page 25 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Parameter setting range: Name Default parameters Program parameter range Sterilizing Temp. 134℃ 115℃～135℃ Sterilizing Time 4min 1min～6000min Sterilization pressure It changes automatically with the No modification is setting change of sterilization recommended temperature Chamber cover opening 99℃...
  • Page 26 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Name Default parameters Program parameter range Sterilizing Temp. 134℃ 115℃～135℃ Sterilizing Time 4min 1min～6000min Sterilization pressure It changes automatically with the No modification is setting change of sterilization recommended temperature Chamber cover opening 99℃ 40℃～99℃...
  • Page 27 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Note: In the program, if the item is 0, such as 0 times of positive pressure pulsation, the next function will be automatically entered in order not to perform this function. Drying is atmospheric pressure drying, that is, drying under the same internal chamber pressure and external pressure.
  • Page 28 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 6.3 Click program to return to the program interface 6.4 After the sterilization is started, the interface will jump to the digital query interface (after the sterilization is started, the default is the digital query screen) 6.5 Click the curve to pop up the following interface:...
  • Page 29 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 6.7 Click stop program to pop up the following interface: Selection confirmation: stop sterilization, return to program interface (if the original fabric program is returned to the fabric program interface), select Cancel and maintain the original.
  • Page 30 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 7.1 After the end of the program, it will automatically jump to the "door operation" interface. See operation in "2". The door opening mode is "open sterilization room door". 8.Take out the sterilized articles 1) Heat insulating gloves should be worn when taking out sterilized articles from the sterilization chamber, and hands can be put into the sterilization chamber after the steam disperses.
  • Page 31 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 1.1.Click password setting to open the following interface: The operator modifies his / her password. After the modification, click confirm to modify and click back to return to the operator management interface 1.2 .Click the alarm information to pop up the following interface: It can query the fault information that has occurred up to the current time 1.3.Click switch to technologist, administrator and engineer in the following line to...
  • Page 32 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 2.1. Click parameter setting to open the following interface: Set the parameters related to sterilization (it is recommended not to set this parameter). The setting range is as follows: Name Default parameters New program parameter...
  • Page 33 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A fluctuation Upper limit value of positive pressure 140kPa 100～170kPa fluctuation Water tank temperature on 45℃ 45～65℃ Rapid ventricular septum 50 second 30～90 seconds Lower limit of positive pressure pulse 102kPa 100～110kPa drying Lower limit value of negative pressure 10kPa 5～90kPa...
  • Page 34 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A The time can be changed to the current time 2.5. Click the following line to switch to technologist, administrator and engineer to switch to the corresponding function interface. 3. Administrator management interface: In administrator, click manage to open the administrator management interface, as shown in the following figure: 3.1.
  • Page 35 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 3.2.1. When you click modify password, the following interface will pop up (only change the password of the administrator himself) If the number of digits is wrong or the password is wrong, there will be a corresponding prompt;...
  • Page 36 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 3.3 when the alarm information is clicked, it is the same as the alarm information in the operator interface. 3.4. When you click time setting, it is the same as the time setting in the technician interface.
  • Page 37 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A It can modify the language, touch prompt tone, article temperature selection, water source pressure selection, print temperature type, print batch number, printer function, pressure unit, date format and altitude 3.6.1 when setting the printer function, the authority control includes closing, opening and operator control;...
  • Page 38 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Welcome to scan the two-dimensional code, pay attention to the official account of the micrometer. 3.9 When you click the deviation compensation setting, the following interface will pop 3.9.1 Click the correction value in the middle of the above figure to modify, with the correction range of - 5 ~ + 5.
  • Page 39 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A set the item thermometer to enable. After use, the article thermometer should be placed on the storage board and properly kept. If it is not used for a long time, the article thermometer can be set to not be enabled in the user administrator settings menu.

  • Page 40: Maintain&Management

    Horizontal Autoclave BAHZ-401-A & BAHZ-402-A ●The printer prints the F0 value A total of 2 F0 values are printed, one of which is in sterilization state, and the other is F0 value of heating process, sterilization process and cooling process in the program process.
  • Page 41 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Plug filter screen ●Vacuum pump: If the working fluid used is harmful to the human body or the environment, for the sake of safety, clean the pump with clean water before emptying. When emptying, open the screw plug (068a) on the pump cover (061a) to let the liquid flow out.
  • Page 42 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Please do not touch the handle of safety valve directly by hand to avoid steam scald. Screw driver or other strip-shaped objects can be inserted into the pull ring to lift the handle. ●Sterilization filter: the filter works when the air enters into the room. After the gas is filtered, it can effectively prevent the air entering the inner room from polluting the sterilized articles.
  • Page 43 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A name, measuring unit and number of digital measuring instruments; vacuum gauge shall have "negative" sign, accuracy grade and factory number. d: The pressure gauge shall be equipped with safety hole, and dust-proof device shall be installed on the safety hole.
  • Page 44 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 3.Replace the sealing ring ●Open the chamber door → Click to open the door. The vacuum pump starts to extract steam. When the air pressure is less than 100KPA, the valve will open automatically. ○Pull the corner of the seal ring to take it off directly ○Clean the dirt on the sealing ring and the contact part between the cavity and the sealing...

  • Page 45: Troubleshooting

    Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 5.List of consumables: Number Part name Electric heating tube Seal ring Sterilizing filter Article temperature sensor (optional) Troubleshooting Equipment alarm information table: Number Alarm Possible causes Handling suggestions information Sterilization chamber door Check whether the switch not locked or chamber door handle is in place;...
  • Page 46 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A power supply higher than the boiling point temperature during start-up operation; The temperature of sterilization is higher than that of sterilization Inner chamber temperature Plug in the temperature sensor control probe failure again Sterilization is not normally...
  • Page 47 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 2.Analysis and elimination of common faults Instrument Possible causes Handling suggestions failure 1. After the power 1. Check whether 380V power 1. Check touch screen power is turned on, the supply is normal supply touch screen does 2.
  • Page 48 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A does not work when it is powered solenoid valve 3. Repair or replace the water inlet 3. Water pump damaged pump 9. The water in the 1. Floating ball level gauge failure 1. Replace or repair the float level steam generator is 2.

  • Page 49: Electrical Wiring Diagram And Component Distribution Diagram

    Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Electrical Wiring Diagram and Component Distribution Diagram 1. Electrical wiring diagram www.biolabscientific.com...
  • Page 50 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Symbol description Number Symbol Name Number Symbol Name Total power supply Electromagnet pull in relay Motor and control Electromagnet protection relay power supply Control power supply Touch screen SPD1 Surge protective PLC1-1 PLC IVC1-1614MAR6 device...
  • Page 51 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A 2. Component distribution diagram 24 V power supply 15V power supply (reserved electromagnet power supply) 5 V power supply Solid state relay www.biolabscientific.com...

  • Page 52: Pipeline Diagram

    Horizontal Autoclave BAHZ-401-A & BAHZ-402-A Pipeline Diagram Chamber distilled water Blowdown and Drain tap water drainage of evaporator outlet www.biolabscientific.com...
  • Page 53 Horizontal Autoclave BAHZ-401-A & BAHZ-402-A BIOLAB SCIENTIFIC LTD. 3660 Midland Avenue, Suite 300, Toronto, Ontario M1V 0B8 Canada Email: contact@biolabscientific.com Tel: +1 707 533 1445 Website: www.biolabscientific.com www.biolabscientific.com...

This manual is also suitable for:

Bahz-402-a

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