Shodex Asahipak GS-HQ Series Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex Asahipak GS-HQ series is polymer-based size
exclusion chromatography (SEC) columns. With the choice of eluent, the column provides multimode features
of reversed phase chromatography, hydrophilic chromatography (HILIC), and ion exchange chromatography in
addition to SEC. The column series is suitable for the separations of peptides or nucleic acids with similar
molecular weights.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
F7600005 Asahipak GS-220 HQ
F7600006 Asahipak GS-320 HQ
F6710019 Asahipak GS-2G 7B
Base Material : Spherical porous particles of polyhydroxymethacrylate
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Methanol = 70/30
4. Usable Conditions
4.1 System Settings
Product Name
Asahipak GS-220 HQ
Asahipak GS-320 HQ
Asahipak GS-2G 7B
Operation Manual
Shode Asahipak GS-HQ series
Column Size
Particle
(mm)
I.D. Length
7.5 300
7.5 300
7.5 50
Flow Rate (mL/min)
Recommended
0.4 - 0.6
-
Resonac Corporation (https://www.shodex.com/)
Theoretical
Size Plate Number
(μm) (Per Column)
6 ≥ 19,000
6 ≥ 19,000
9 (Guard Column)
* Reference value only / Measured with pullulan
Maximum
Pressure
Maximum
(MPa/column)
6.0
1.0
5.0
- -
ver.20221202E
Target Molecular Exclusion
Weight Range * Limit
300 - 3,000 7,000
300 - 20,000 40,000
- -
pH Temperature
Range Range (°C)
2 - 9
4 - 60
2 - 12
*
1