Shodex SUGAR SC1211 Operation Manual

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(Please read this manual carefully to achieve the best and consistent column performance for a long time)

Important Handling Instructions

Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex SUGAR SC1211 is a high performance
chromatography column which is specifically designed for the separations and analysis of sugars. A strong acid
cation exchange type packing gel, prepared from a rigid resin of styrene divinylbenzene copolymer provides a
mixed separation mode. The analytes are separated by a combination of ligand exchange and hydrophilic
chromatography (HILIC) modes. It is capable of separating and analyzing sugar alcohols from each other.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F7001400
SUGAR SC1211
F6700120
SUGAR SC1211G 4A
Base Material
: Spherical porous particles of styrene divinylbenzene copolymer modified with sulfo group
(counter ion: Ca
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile = 75/25
Operation Manual
Shodex
SUGAR
Column Size (mm)
I.D.
6.0
4.6
2+
)
SHOWA DENKO K.K. (https://www.shodex.com/)
SC1211
Particle
Size (μm)
Length
250
6
10
10
ver.210629E
Theoretical Plate Number
(Per Column)
≥ 5.500
(Guard Column)
1

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Summary of Contents for Shodex SUGAR SC1211

  • Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex SUGAR SC1211 is a high performance chromatography column which is specifically designed for the separations and analysis of sugars. A strong acid cation exchange type packing gel, prepared from a rigid resin of styrene divinylbenzene copolymer provides a mixed separation mode.
  • Page 2 If using acetonitrile, make sure that the final acetonitrile concentration is less than 40 % (v/v). (7) When a sample contains a large amount of organic acids, use an OH-form anion exchange resin to remove them. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 3 This may result in creating an empty space in the column and deteriorates the column. Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 4 (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
  • Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.

This manual is also suitable for:

Sugar sc1211g 4aF7001400F6700120

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