Shodex SUGAR SP0810 8C Operation Manual

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(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex SUGAR SP0810 8C is a high performance
chromatography column which is specifically designed for the separations and analysis of sugars. A strong acid
cation exchange type packing gel, prepared from a rigid resin of styrene divinylbenzene copolymer provides a
mixed separation mode. The analytes are separated by a combination of ligand exchange and size exclusion
modes. It is capable of rapidly separating and analyzing monosaccharides or disaccharides from each other,
and also sugar alcohols.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F6378106
SUGAR SP0810 8C
Base Material
: Spherical porous particles of styrene divinylbenzene copolymer modified with sulfo group
(counter ion: Pb
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water
Operation Manual
Shodex
SUGAR
Column Size
(mm)
I.D.
Length
8.0
100
2+
)
SHOWA DENKO K.K. (https://www.shodex.com/)
SP0810 8C
Theoretical Plate
Particle size
Number
(μm)
(Per Column)
7
≥ 3,000
*
Reference value only / Measured with pullulan
ver.211015E
*
Exclusion Limit
1,000
1

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Summary of Contents for Shodex SUGAR SP0810 8C

  • Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex SUGAR SP0810 8C is a high performance chromatography column which is specifically designed for the separations and analysis of sugars. A strong acid cation exchange type packing gel, prepared from a rigid resin of styrene divinylbenzene copolymer provides a mixed separation mode.
  • Page 2 Recommended Maximum Recommended Maximum SUGAR SP0810 8C 0.5 - 1.0 70 - 85 When analyzing under 40 °C, set the flow rate at less than 0.5 mL/min to reduce the column load. Increased temperature slows down the formation of sugar anomers, and thus prevents peak splitting.
  • Page 3 When replacing the solvent, set the column temperature at 70 - 95 °C and start the system at 0.2 - 0.3 mL/min. Recommended solvent volume to introduce at each step is 3 to 5 times of the column volume. Attention! · Frequent eluent replacement may damage the column, and thus not recommended. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 4 9. Column Inspection Inspection method is described in the Certificate of Analysis (CoA). Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations. (1) Theoretical Plate Number (N) (2) Asymmetry Factor (Fas) SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 5 (3) When disposing the column, please follow a proper waste disposal method specified by your local regulations. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
  • Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.

This manual is also suitable for:

F6378106

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