Shodex OHpak SB-800 HQ Series Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex OHpak SB-800 HQ series is size exclusion
chromatography columns used with aqueous solvents. The column series is suitable for separations of various
water-soluble polymers, proteins, and oligomers. It is also suitable for molecular weight distribution analysis of
polymers. Please select columns with target molecular weight ranges (see below) that meet the molecular
weight range of your samples.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
OHpak SB-802 HQ
F6429100
F6429101 OHpak SB-802.5 HQ
F6429102 OHpak SB-803 HQ
F6429103 OHpak SB-804 HQ
F6429104 OHpak SB-805 HQ
F6429105 OHpak SB-806 HQ
OHpak SB-806M HQ
F6429106
OHpak SB-G 6B
F6709430
F6429108 OHpak SB-807 HQ
F6709431 OHpak SB-807G
SB-806M HQ is a mixed-gel column, provides a wider molecular-weight analysis range.
Operation Manual
Shodex OHpak SB-800 HQ series
Column Size
Particle
(mm)
Size
(μm)
I.D. Length
8.0 300 8
8.0 300 6
8.0 300 6
8.0 300 10
8.0 300 13
8.0 300 13
8.0 300 13
6.0 50 10
8.0 300 35
8.0 50 35
Resonac Corporation (https://www.shodex.com/)
Theoretical
Target Molecular Weight
Plate Number
Range
(Per Column)
≥ 12,000 200 - 1,000
≥ 16,000 500 - 10,000
≥ 16,000 1,000 - 100,000
≥ 16,000 5,000 - 400,000
≥ 12,000 100,000 - 1,000,000
≥ 12,000 100,000 - (20,000,000)
≥ 12,000 500 - (20,000,000)
(Guard Column)
≥ 1,500 500,000 - (500,000,000)
(Guard Column)
*1 Reference value only / Measured with pullulan
ver.20221202E
Exclusion Limit
*1
1,000
10,000
100,000
1,000,000
(4,000,000)
*2 (20,000,000)
*2 (20,000,000)
- -
(500,000,000)
*2
- -
*2 Estimated value
*1
*2
*2
*2
*2
1