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（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex product. Shodex IEC SP-825 is a strong cation exchange column
filled with hydrophilic polymer-based gel modified with sulfopropyl group. It is an ideal column for analyzing
various proteins, peptides, and components of nucleic acids as it has a low adsorption tendency towards
hydrophobic compounds. The polymer-based gel allows its use at a wide pH range condition.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6118250
IEC SP-825
Base Material
: Spherical porous particles of polyhydroxymethacrylate modified with sulfopropyl group
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : 50 mM Sodium Sulfate aq. Solution
Operation Manual
Shodex
IEC SP-825
Column Size (mm)
I.D.
Length
8.0
75
SHOWA DENKO K.K. (https://www.shodex.com/)
Particle
Theoretical Plate
Size
Number
(μm)
(Per Column)
8
≥ 2,000
ver.211129E
Ion Exchange
Capacity
(meq/g)
0.40
1
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Summary of Contents for Shodex IEC SP-825
- Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex IEC SP-825 is a strong cation exchange column filled with hydrophilic polymer-based gel modified with sulfopropyl group. It is an ideal column for analyzing various proteins, peptides, and components of nucleic acids as it has a low adsorption tendency towards hydrophobic compounds.
- Page 2 The content may have been changed, absorbed moisture, or has been contaminated. · Always use freshly prepared solvents. Solvents stored for a long time may have changed their compositions and may influence elution patterns and/or damage the column. Note · Use of on-line degasser is recommended. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 3 This may result in creating an empty space in the column and deteriorates the column. Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 4 (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
- Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.
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