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Columns manufactured by Showa Denko K.K Japan
Made in Japan
HPLC Columns
MANUAL
Asahipak ODP-50
(C8P, C4P series)
Shodex HPLC Columns
Europe, Middle East, Africa, Russia
For technical support please use
contact details shown below:
SHOWA DENKO EUROPE GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich, Germany
E-mail:
support@shodex.de
Phone:
+49 (0)89 93 99 62 37
www.shodex.de
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Related Manuals for Shodex Asahipak ODP-50 series
Summary of Contents for Shodex Asahipak ODP-50 series
- Page 1 HPLC Columns MANUAL Asahipak ODP-50 (C8P, C4P series) Shodex HPLC Columns Europe, Middle East, Africa, Russia For technical support please use contact details shown below: SHOWA DENKO EUROPE GmbH Shodex Business Konrad-Zuse-Platz 3 81829 Munich, Germany Columns manufactured by Showa Denko K.K Japan E-mail: support@shodex.de...
- Page 3 (Please read this manual carefully before using the column to keep its good performance and life.) NOTE : Although Shodex Asahipak Columns are designed to operate at extremes of pH, the user should be aware that components of the HPLC system may be susceptible to acidic or basic conditions.
- Page 4 Asahipak ODP-50 (C8P, C4P series) Manual popular "C8" or "octyl" columns. Thus Asahipak columns for reversed phase chromatography combine a range of hydrophobic character commonly found in silica packings with the high chemical and physical stability of polymer based particles. The user should read this entire manual before using the column, especially for the first time.
- Page 5 Asahipak ODP-50 (C8P, C4P series) Manual Table 1. Specifications of Asahipak ODP, C8P and C4P columns Flow rate Column Particle size Column dimensions NTP* Normal Maximum (µm) (IDxL.mm) (ml/min) (ml/min) 4.6 x 250 >14000 0.5 – 0.8 4.6 x 150 >9000 0.5 –...
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Page 6: Column Installation
Asahipak ODP-50 (C8P, C4P series) Manual 4. Column installation 4.1 Direction of flow Install and use the column with mobile phase flowing in the direction indicated on the column tag. If the column shows very high resistance to flow, it may be partially plugged up. This is probably the end of the service life of the column. - Page 7 Asahipak ODP-50 (C8P, C4P series) Manual 4.5 Connection of column to HPLC Connect the column inlet to the tube from the injector. Do not turn the fitting tight. Turn on the pump flow to one half the lower operating range in Table 1. Allow a small amount of the mobile phase to leak out and displace any air bubbles from the cavities in the column inlet.
- Page 8 Asahipak ODP-50 (C8P, C4P series) Manual For control of pH within the range of pH 2 to pH 13, phosphate, acetate, tris-HCl, NaCl, KCl, , and trifluoroacetic acid buffers can be used. Please consult your Asahipak representative if you have questions about buffering agents not listed above. It is important to note that the mobile phase should be filtered before use as described in section 5.1.
- Page 9 Asahipak ODP-50 (C8P, C4P series) Manual 7.2 Asahipak ODP-50, C8P-50 and C4P-50 There are no restrictions on the rate of change of mobile phase composition provided that the column is operating within the specified range of flow rate and pressure, Section 3 and Table 1. 8.
- Page 10 Asahipak ODP-50 (C8P, C4P series) Manual could precipitate during standing or by adding the 50% methanol, see Section 6. Also it is important that the column not be permitted to dry out. 11. Sample preparation In general, the sample should be dissolved in the mobile phase. Dissolving the sample in the mobile phase often avoids large "system peaks"...
- Page 11 *No licence or immunity under any patent is granted or implied by our sales of any material. * Shodex Asahipak products in this document are for laboratory use only. They are not intended for medicinal or food use. We assume no responsibility if these products are used for medicinal or food purposes, or are misused in any way.
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