Shodex Asahipak GF-HQ Series Operation Manual

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(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex™ product. Shodex Asahipak GF-HQ series is polymer-based size
exclusion chromatography columns. They work well with both aqueous and organic solvents.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F7600000
Asahipak GF-210 HQ
F7600001
Asahipak GF-310 HQ
F7600002
Asahipak GF-510 HQ
F7600004
Asahipak GF-7M HQ
F6710018
Asahipak GF-1G 7B
GF-7M HQ is a mixed-gel column, suitable for the analysis of wide molecular weight ranges.
Operation Manual
Shodex
Asahipak
Column Size
Particle
(mm)
Size (μm)
I.D.
Length
7.5
300
7.5
300
7.5
300
7.5
300
7.5
50
SHOWA DENKO K.K. (https://www.shodex.com/)
GF-HQ series
Theoretical
Plate Number
(Per Column)
5
≥ 19,000
5
≥ 19,000
5
≥ 19,000
9
≥ 13,000
9
(Guard Column)
*1
Reference value only / Measured with pullulan
ver.211228E
Target Molecular
Exclusion
Weight Range
*1
Limit
300 - 4,000
300 - 30,000
40,000
5,000 - 200,000
300,000
300 - (10,000,000)
*2
(10,000,000)
-
*2
Estimated value
*1
9,000
*2
-
1

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Summary of Contents for Shodex Asahipak GF-HQ Series

  • Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex™ product. Shodex Asahipak GF-HQ series is polymer-based size exclusion chromatography columns. They work well with both aqueous and organic solvents. 2. Column Components 3.
  • Page 2 (4) Surfactants such as SDS and Brij-35 can also be added to the eluent. However, since surfactants tend to remain on the column, solvent replacement after their use takes a longer time than replacement from general solvents. The replacement time can be shorten by using 30 - 50 % (v/v) methanol. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 3 5,000 - 25,000 ≤ 0.5 % 25,000 - 200,000 ≤ 0.25 % 200,000 - 2,000,000 ≤ 0.1 % 2,000,000 ≤ ≤ 0.05 % Note · Use of guard column is recommended to protect the analytical column. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 4 This may result in creating an empty space in the column and deteriorates the column. Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 5 (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 6 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
  • Page 7 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.

This manual is also suitable for:

Asahipak gf-210 hqAsahipak gf-310 hqAsahipak gf-510 hqAsahipak gf-7m hqAsahipak gf-1g 7bF7600000 ... Show all

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