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Shodex OHpak SB-804 HQ SR Operation Manual

Shodex OHpak SB-804 HQ SR Operation Manual

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(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex™ product. Shodex OHpak SB-804 HQ SR is a size exclusion
chromatography column used with aqueous solvents. It is a special specification column of OHpak™
SB-804 HQ.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6429109
OHpak SB-804 HQ SR
F6709430
OHpak SB-G 6B
Base Material
: Spherical porous particles of polyhydroxymethacrylate
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water (SB-804 HQ SR)
0.02 % Sodium Azide aq. Solution (SB-G 6B)
Operation Manual
Shodex
OHpak
Column Size
Particle
(mm)
I.D.
Length
8.0
300
6.0
50
SHOWA DENKO K.K. (https://www.shodex.com/)
SB-804 HQ SR
Theoretical
Size
Plate Number
(μm)
(Per Column)
10
≥ 16,000
10
(Guard Column)
*1
Reference value only / Measured with pullulan
ver.211026E
Target Molecular
Exclusion
Weight Range
*1
Limit
5,000 - 400,000
1,000,000
-
-
*1
1

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Summary of Contents for Shodex OHpak SB-804 HQ SR

  • Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex™ product. Shodex OHpak SB-804 HQ SR is a size exclusion chromatography column used with aqueous solvents. It is a special specification column of OHpak™...
  • Page 2: Eluent Preparation

    Flow Rate (mL/min) Maximum Pressure Temperature Product Name pH Range (MPa)(Per Column) Range (°C) Recommended Maximum OHpak SB-804 HQ SR 0.5 - 1.0 3 - 10 4 - 70 OHpak SB-G 6B 4.2 Usable solvents Maximum Usable Solvents (%) Product Name Methanol...
  • Page 3: Column Installation

    (2) Make sure to insert the tubing all the way to the end fitting and secure it with the male nut. It is important that there is no extra space between the tubing and the column side of the end fitting. Presence of an extra space will let the sample to spread out and may result in wide peaks. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 4 When washing the column, let the washing solution flow from the column outlet go directly into the waste container and not let the solution go through the detector. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
  • Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.