Shodex Asahipak GF-HQ Series Operation Manual

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(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)

Important Handling Instructions

Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.

Before Using the Column

(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.

1. Introduction

Thank you for purchasing the Shodex product. Shodex Asahipak GF-HQ series is polymer-based size
exclusion (SEC) chromatography columns. Because of their high solvent tolerance, they work well with both
aqueous and organic solvents.

2. Column Components

Please refer to the Shodex website: https://www.shodex.com/en/da/07.html

3. Column Specifications

Product
Product Name
Code
F7600000
Asahipak GF-210 HQ
F7600001
Asahipak GF-310 HQ
F7600002
Asahipak GF-510 HQ
Asahipak GF-7M HQ
F7600004
Asahipak GF-1G 7B
F6710018
GF-7M HQ is a mixed-gel column, provides a wider molecular-weight analysis range.
Base Material
: Spherical porous particles of polyvinyl alcohol
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Methanol = 70/30 (GF-310 HQ, GF-510 HQ, GF-7M HQ, GF-1G 7B)
Water (GF-210 HQ)
Operation Manual
Shodex Asahipak GF-HQ series
Column Size
Particle
(mm)
Size
(μm)
I.D.
Length
7.5
300
7.5
300
7.5
300
7.5
300
7.5
50
Resonac Corporation (https://www.shodex.com/)
Theoretical
Target Molecular
Plate Number
(Per Column)
5
≥ 19,000
5
≥ 19,000
5
≥ 19,000
9
≥ 13,000
300 - (10,000,000)
9
(Guard Column)
*1
Reference value only / Measured with pullulan
ver.20221202E
Exclusion
Weight Range
*1
Limit
300 - 4,000
9,000
300 - 30,000
40,000
5,000 - 200,000
300,000
*2
(10,000,000)
-
*2
Estimated value
*1
*2
-
1

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Summary of Contents for Shodex Asahipak GF-HQ Series

  • Page 1 Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number. 1. Introduction Thank you for purchasing the Shodex product. Shodex Asahipak GF-HQ series is polymer-based size exclusion (SEC) chromatography columns. Because of their high solvent tolerance, they work well with both aqueous and organic solvents.
  • Page 2 · When using highly corrosive salts such as sodium chloride, wash out the salts at the end of analysis. The metal parts of the devices and/or the columns may rust. Resonac Corporation (https://www.shodex.com/)
  • Page 3 · When a sample is dissolved in a solvent other than the eluent and if the sample matrix contains components which do not dissolve in the eluent fully, precipitates may form and clog the column. Note · Use of a guard column is recommended to protect the analytical column. Resonac Corporation (https://www.shodex.com/)
  • Page 4 Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. Resonac Corporation (https://www.shodex.com/)
  • Page 5 Securely tighten the end caps and store the column at a location with stable temperature (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside the column to dry. It can damage the column. Resonac Corporation (https://www.shodex.com/)
  • Page 6 9. Column Inspection Please refer to the inspection method described in the CoA. At Shodex, “half width method” is adopted for the calculation of plate count and “asymmetry factor (Fas)” is adopted for the calculation of peak symmetry. Please refer to the Shodex website for the detail: https://www.shodex.com/en/da/07.html...

This manual is also suitable for:

Asahipak gf-210 hqAsahipak gf-310 hqAsahipak gf-510 hqAsahipak gf-7m hqAsahipak gf-1g 7bF7600000 ... Show all

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