Shodex RSpak DS-413 Operation Manual

（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex RSpak DS-413 is a polymer-based reversed phase
chromatography column. It can be used in a wide pH range from acidic to alkaline (pH 1 - 13).

2. Column Components

3. Column Specifications

Product
Product Name
Code
F7001012 RSpak DS-413
Base Material : Spherical porous particles of styrene divinylbenzene copolymer
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile/THF = 40/30/30

4. Usable Conditions

Product Name
Recommended
RSpak DS-413
Operation Manual
TM
Shodex
Column Size (mm)
I.D.
4.6
Flow Rate (mL/min)
Maximum
0.6 - 1.0 1.0
*
SHOWA DENKO K.K. (https://www.shodex.com/)
TM
RSpak DS-413
Particle
Size (μm)
Length
150
Maximum Pressure
(MPa)
(Per Column)
20
Eluent without THF, the maximum usable temperature is 70 °C.
Theoretical Plate Number
(Per Column)
3.5 ≥ 11,000
Temperature
pH Range
Range
1 - 13
ver.211129E
(°C)
*
20 - 50
1