Shodex GPC KF-400HQ Series Operation Manual

（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex GPC KF-400HQ series is a size exclusion
chromatography semi-micro column used with organic solvents. The columns support higher sensitive and
higher resolution analysis than standard columns and they are suitable of separation of organics and oligomers.
The columns with different exclusion limits are available. Please select columns with target molecular weight
ranges (see below) that meet the molecular weight range of your samples.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F6028111 GPC KF-401HQ
F6028112 GPC KF-402HQ
F6028114 GPC KF-402.5HQ
F6028116 GPC KF-403HQ
F6700300 GPC KF-G 4A
Base Material : Spherical porous particles of styrene divinylbenzene copolymer
Column Housing : SUS-316
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Tetrahydrofuran (THF)
Operation Manual

Shodex GPC KF-400HQ series
Column Size (mm)
Particle
Size (μm)
I.D. Length
4.6 250
4.6 250
4.6 250
4.6 250
4.6 10
SHOWA DENKO K.K. (https://www.shodex.com/)
Theoretical Plate
Number
(Per Column)
3 ≥ 25,000
3 ≥ 25,000
3 ≥ 25,000
3 ≥ 25,000
8 (Guard Column)
* Reference value only / Measured with polystyrene
ver.211004E
Target Molecular Exclusion
Weight Range * Limit
100 - 700 1,500
200 - 1,500 4,000
300 - 10,000 20,000
600 - 50,000 70,000
- -
*
1