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（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex product. Shodex RSpak KC-811 is a high performance
chromatography column which is specifically designed for the separations and analysis of organic acids. The
column is packed with a strong acid cation exchange gel prepared from a rigid resin of styrene divinylbenzene
copolymer and it works under ion exclusion chromatography mode. It is capable of separating and analyzing
multiple organic acids simultaneously.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6378030
RSpak KC-811
F6700030
RSpak KC-G 6B
F6700010
RSpak KC-G 8B
Please select KC-G 6B for the samples containing less matrix and KC-G 8B for the samples containing more matrix.
Base Material
: Spherical porous particles of styrene divinylbenzene copolymer modified with sulfo group
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : 0.1 % Phosphoric Acid aq. Solution
Operation Manual
Shodex
RSpak
Column Size (mm)
I.D.
Length
8.0
300
6.0
8.0
SHOWA DENKO K.K. (https://www.shodex.com/)
KC-811
Particle size
(μm)
6
50
10
50
13
ver.211209E
Theoretical Plate Number
(Per Column)
≥ 17,000
(Guard Column)
(Guard Column)
1
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Summary of Contents for Shodex RSpak KC-811
- Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex RSpak KC-811 is a high performance chromatography column which is specifically designed for the separations and analysis of organic acids. The column is packed with a strong acid cation exchange gel prepared from a rigid resin of styrene divinylbenzene copolymer and it works under ion exclusion chromatography mode.
- Page 2 (8) When a sample contains hydrophobic substances or surfactants, use a reversed-phase solid phase extraction to remove them. Note · Use of guard column is recommended to protect the analytical column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 3 When replacing the solvent, set the column temperature at 50 °C and start the system at 0.2 - 0.3 mL/min. Recommended solvent volume to introduce at each step is 3 to 5 times of the column volume. Attention! · Frequent eluent replacement may damage the column, and thus not recommended. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 4 (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
- Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.
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