Preparing The Reagent Kit; Precautions; Reconstitution - Merck Milliflex Quantum User Manual

10. Preparing the Reagent Kit

10.1 Precautions

• Do not use any expired kit component.
• Remove the kit from the refrigerator one hour before starting the reconstitution.
• R econstitute under a laminar flow hood or a microbiological safety cabinet.
• During the seven-day shelf life, do not let the staining solution remain at room
temperature for more than four hours per day.
• When removing the staining solution from the refrigerator, wait one hour before
performing a staining.
• Use appropriate personal protective equipment when using the staining solution.
• M ix the staining solution before performing the first test of the day.

10.2 Reconstitution

Reconstitute the staining solution following the procedure on the Staining Buffer Vial (vial 3)
before the first use of the reagent kit. The reconstitution date may be noted on the
Staining Buffer (vial 3) label.
Store the reconstituted Staining Solution at 2 to 8 °C. 48 tests can be performed with the
reconstituted Staining Solution. The approximate number of remaining tests is indicated
on the Staining Buffer (vial 3) label.
The shelf life of the reconstituted Staining Solution is 7 days.
NOTE: T o extend the shelf life, the reconstituted Staining Solution can be distributed in up to four aliquots.
These aliquots can be stored up to four weeks in the freezer. Once thawed, the aliquot must be
stored at 2 to 8 °C, protected from light. The shelf-life of the thawed aliquot is seven days.
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