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Summary of Contents for Amos AST 550
- Page 1 AST 550 (Version：AMOSAST550OP20240204） Fully-automatic Cryostat Microtome Operation Manual AMOS SCIENTIFIC PTY.LTD. ABN37 159 778 140 www.amos-scientific.com...
- Page 2 Presented in this Operation manual are the structure, performance and user’s guideline. Read carefully prior to operating the equipment.
- Page 3 Foreword AST550 Cryostat Microtome is a fully- automatic microtome designed with input from global customers, capable of meeting diverse requirements in routine sectioning. To ensure the machine's smooth, durable, and safe functioning, it is essential to read this operational manual thoroughly. Our company takes responsibility for maintenance products...
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Page 4: Table Of Contents
Contents 1. Safety Notes……………………………………………………..……..1 1.1 Overview……………………………………………………………………..……….1 1.2 Safety Warning………………………………………………………………..…..….1 1.3 Safety Device………………………………………………......……..…..….2 2. Performance & Parameters……………………………......4 2.1 Overview of Instrument Component………………………………….….…...……….4 2.2 Performance Index……………………………………………………………..……..5 2.3 Technical Parameters…………………………………………………………..……..5 3. Preparation before operating…………………………………….….…..7 3.1 Requirement of Installation ………………………………………………..……..….7 3.2 Standard Delivery…………………………………………………………....….7 3.3 Installation ………………………………………………………………....….8 3.4 Electrical Connection…………………………………………………....….10 4. -
Page 5: Safety Notes
1. Safety Notes 1.1 Overview The Operation Manual contains important safety instructions and information. The operation manual is an important part of the instrument, which the operator must read carefully prior to startup to ensure safe operation. It performs a critical role in maintaining personal safety and preventing equipment damage. -
Page 6: Safety Device
● Turn the handwheel in a clockwise direction to maintain the desired sectioning results. Incorrect rotation may affect the sectioning outcome. ● While sectioning, avoid frequent back-and-forth rotation of the handwheel when it is positioned at the top or bottom, as this may impact the thickness of the sections. ●... - Page 7 Emergency Stop Function As shown in the diagram (2), the emergency stop switch is used to immediately cut off the main power of the instrument and stop all activity when pressed. To release it, rotate the switch in the direction indicated by the arrow until the button pops out.
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Page 8: Performance & Parameters
2. Performance & Parameters 2.1 Instrument Components Overview Display Control Panel Heating Glass Control Panel E-stop switch Stationary Heat Control Panel Extractor Quick Freeze Shelf Specimen Clamp Blade Holder Peltier on freeze shelf- Freeze Lock Lever Area Waste Container Handwheel Tool Shelf ---4--- Ver:AMOSAST550OP20240204... -
Page 9: Performance Index
2.2 Performance Index AST550 is a fully automatic cryostat microtome, the movement and feed of the sample are automatically controlled by a stepper motor, ensuring higher sectioning precision and simplified operation. Below are some performance features of the device: ⊙ The instrument adheres to ergonomic design principles for a more aesthetically pleasing appearance, crafted through CNC for precision. - Page 10 ⊙ Electric coarse feed：Slow 300µm/s±30µm/s Rapid 900µm/s±30µm/s ⊙ Section thickness range： 0 to 100µm 0 to 3µm， in 0.5µm increments 3 to 10µm, in 1µm increments 10 to 20µm, in 2µm increments 20 to 100µm，in 5µm increments ⊙ Trimming thickness range: 10 to 600µm 10 to 50µm，...
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Page 11: Preparation Before Operating
3. Preparation before operating 3.1 Installation site requirement ⊙ This machine is a mobile floor-standing unit equipped with four caster wheels at the bottom and two fixed support feet. The front two casters are used when movement is required. After removing the packaging, move the machine to the desired location. Once in position, adjust the two fixed support feet to deactivate the front casters. -
Page 12: Installation
3.3 Installation 3.3.1 Unpacking As showed in the diagram，the following is the instrument accessories： ⊙ Carton Cover（1） ⊙ Upper Support（2） ⊙ Corner Support *1（3） ⊙ Carton body（4） ⊙ Glass Support（5） ⊙ Corner Support *2（6） ⊙ Corner Support *3（7） ⊙ Handwheel（8） ⊙... - Page 13 3.3.2 Handwheel assembly ·Take out the handwheel accessories from handwheel box Tighten the handwheel shaft (14) · into handwheel (13) with wrench . In the following order, insert · bearing (15) , handwheel sleeve (16) Tighten them with M5 × 16 and handle pad (17).
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Page 14: Electrical Connection
3.4 Electrical Connection ●The input voltage is set before exiting factory. Prior to installation, please check that this setting complies with the local power requirements of your laboratory, as incompatible voltage may cause damage to the equipment. ● For safety during instrument operation, the power supply must be equipped with a grounding wire, adhering to safety standards. -
Page 15: Operation
4. Operation 4.1 Control panel function & Control All the parameters are set and displayed via control panel. After power on, use the control panel to operate: 4.1.1 Display Panel Display area shows information such Indicator light The area is to set the value of section thickness, trimming... - Page 16 The two keys are used for setting “ ＋ ” , “ － ” section/trimming thickness , time and temperature. Section thickness value: 0 to 100µm 0 to 3µm，in 0.5µm increments 3 to 10µm，in 1µm increments 10 to 20µm，in 2µm increments 20 to 100µm, in 5µm increments Trimming thickness value: 10 to 600µm 10 to 50µm，in 5µm increments...
- Page 17 is a cycle key to select desired time mode Short press to toggle between real-time, real-time clock setting, defrost time setting, disinfection time setting, sleep time setting, wake-up time setting, and day setting. is the real-time indicator light When in real-time setting, the real-time clock indicator is illuminated.
- Page 18 Keyboard locking key This key is used to lock the keyboard. Hold on this button until the icon " " shows up on display panel (As shown in left diagram), the keyboard has been locked. All other keys (other than itself and the lamp key) are disabled at this moment and release the lock by long press the locking key again.
- Page 19 4.1.3 Right control panel Mode selection Run/Stop between manual motorized mode and automatic Speed adjusting button under automatic mode Mode selection between manual and automatic Select the mode by pressing this button. Auto-run/ Stop button in automatic mode Long press this button to activate the automatic function. During automatic sectioning operation, a short press of this button will stop the automatic motion.
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Page 20: Installation Of Blade Holder
4.2 Installation of blade holder As shown in the diagram, the blade holder consists of the following components: base (1), vertical slider (2), rotating slider (3), movable knife clamp (4), fixed knife clamp (5), anti-curling plate (6), lever 1 (7), lever 2 (8), lever 3 (9), lever 4 (10), anti-curling plate locking knob (11), anti-curling plate height adjustment knob (12). - Page 21 Installation of blade holder and base As shown in the diagram, slide the blade holder (13) horizontally along the track on the cryostat microtome (14), rotate lever 2 (8) to secure it, and adjust the adjustable handle on lever 2 (8) to a horizontal position.
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Page 22: Clamping The Specimen & Inserting The Blade
4.3 Clamping the Specimen & Inserting the Blade Clamping the specimen Lift the sample clamp lever upwards as shown in the diagram, place the sample into the sample clamp (1), and release the sample clamp lever (2). ● Always clamp the specimen before installing the blade to avoid injury . -
Page 23: Temperature Setting
4.4 Temperature setting 4.4.1 Specimen clamp temperature setting -After pressing , as shown in the left diagram, the border of Board area Border the temperature area will be bolded. When the display shows it entered specimen clamp temperature display mode. -Press to enter temperature setting. -
Page 24: Time Setting
4.4.4 Ambient temperature Board area Border 区域边框 Press and the border of the temperature area will be bolded. When the display shows , it entered ambient temperature display mode. The temperature on display reflects the actual ambient temperature. Ambient Temperature 环境温度... - Page 25 4.5.2 Defrosting time setting Press and the border of the time display area will be bolded. When the light is on, it enters defrost time mode as the left diagram illustrates. Press to enter defrost time setting. The value can be set through ；Press to switch to minute setting, then press again to switch to...
- Page 26 4.5.4 Sleep time setting Press and the border of the time display area will be bolded. When the light is on, it enters sleep time mode as the left diagram illustrates. press to enter sleep time setting. The value can be set through ；...
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Page 27: Section/Trimming Setting
4.6 Section/Trimming setting ⊙ Mode selection Select the desired mode between manual and automatic mode. When in manual mode, the icon " " appears on the top of the retraction value display (as left diagram shows) , In this mode, pressing the button for automatic sectioning will not work, and automatic mode cannot be initiated. -
Page 28: Retraction Setting
⊙ Section (in motorized mode) Long press the button corresponds to the operation indicator light turning on, indicating the start of the operation. A short press of this button will stop the operation. During Counter operation, all other buttons, except the speed adjustment button , are non-responsive. -
Page 29: Cleaning & Maintenance
1) Selecting the appropriate working temperature for the cryochamber. 2) Executing precise sectioning operations. 3) Fine-tuning the anti-curling plate. 4) Using a sharp sectioning knife with an appropriate cutting angle. 5) Ensuring the blade is securely clamped. 6) Ensuring the sample is properly secured. Choosing the angle between the sectioning knife and the sample is crucial. -
Page 30: Maintenance
⊙ Cleaning the blade holder Detach all the parts of blade holder as it is shown in the picture and clean all the parts separately, especially the following easily polluted parts: the sliding rail, lever, blade clamp and the joint of the parts. And remember to clean the blade clamp every time before installing a new blade to ensure good sectioning. -
Page 31: Trouble Shooting
6. Trouble Shooting Below you find a list of the problems that most frequently occur. And they are mostly caused by operation, so please read the operation manual carefully before using it. Problem Possible causes Corrective action · The mains cable is not ·Reconnected the main cable or ·... - Page 32 Problem Possible causes Corrective action Wavy sectioning · Improper inclination of the ·Readjust the tilting gradient. · sectioning blade. surface The section flies · It is effected by static. ·Increase the surrounding · away and sticks to the humidity to get rid of the static. microtome or other near objects 如果其它故障或上述故障不能排除，请与公司联系...
- Page 33 Standard accessories list AST550 Accessory Name Notes Cryostat Microtome 1 set Blade Holder 1 set Handling wheel 1 pc Specimen clamp 10 pcs M5 Allen Wrench 1 pc M3Allen Wrench 1 pc 7mm Wrench 1 pc Disposable blade 1 box Power cord 1 pc Brush...
- Page 34 Published by: AMOS SCIENTIFIC PTY.LTD 24/632-643 Clayton Road, Clayton South, VIC 3169 Melbourne, Australia Tel: +61-4-10190188 ABN 37 159 778 140 www.amos-scientific.com Email: info@amos-scientific.com...
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