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Summary of Contents for Amos AEM 460
- Page 1 AEM 460 （Version：AMOSAEM460OP20180306） Semi-automatic Microtome Operation Manual AMOS SCIENTIFIC PTY.LTD. ABN37 159 778 140 www.amos-scientific.com...
- Page 3 Quick release cassette Clamp Display Panel Knife holder Handwheel Locking Mechanism Section Waste tray Separate control panel Smooth-turning handwheel Lever for activating the handwheel brake This Operation manual introduces the instruments component, key features and using notes. Be sure to read these instructions before using the instrument.
- Page 4 Foreward AEM460 Semi-Motorized Microtome is designed for the worldwide user. It can meet the different requirement in the fields of biology, medicine and industry. It is necessary to read this technical manual to ensure that the instrument is snuff, long haul and safely used. Our company is responsible for service after the microtome have been used, our agents have ability to service, the client all of...
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Page 5: Table Of Contents
Contents 1、Safety Notes…………………………………………………………..1 1.1 Overview…………………………………………………………………………….1 1.2 Safety Warning……………………………………………………………………...1 1.3 Safety Devices……………………………………………………………………….3 2、Performance & Parameters…………………………………………4 2.1 Overview-Instrument Components………………………………………………..4 2.2 Performance Index………………………………………………………………….5 2.3 Technical Data……………………………………………………………………….5 3、Preparing before using……………………………………………….6 3.1 Installation Site Requirement………………………………………………………6 3.2 Standard Delivery…………………………………………………………………...6 3.3 Installation…………………………………………………………………………..7 3.4 Electrical Connection………………………………………………………………..8 4、Operation……………………………………………………………...9 4.1 Control Panel Function &Control………………………………………………….9 4.2 Knife Holder inserting……………………………………………………………...12 4.3 Clamping The Specimen……………………………………………………………14 4.4 Specimen Clamps/Knife Inserting…………………………………………………15... -
Page 6: 1、Safety Notes
1、Safety Notes 1.1Overview The Instruction Manual contains important safety instructions and information. The operation manual is an important part of the instrument, which the operator must be read carefully prior to startup and use in order to running safely. It will at a critical moment to ensure personal safety and avoid instrument damage. - Page 7 1.2.2 Warnings-Operation ● Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause serious injury! ● Always remove the knife/blade before detaching the knife holder from the instrument, and put the knives back into the knife box when not in use! ●...
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Page 8: Safety Devices
1.3Safety Devices Handwheel locking mechanism As showed in the Fig, lever（1） and（2） can be to lock the handwheel. To clockwise push lever（1）until it lock the handwheel while specimen place at the top position. And also can clockwise rotate lever （2） about 180 °... -
Page 9: 2、Performance & Parameters
2、 Performance & Parameters 2.1 Overview- Instrument Components Specimen Clamp Adjustor Quick release cassette Clamp Display Panel Knife Guard at knife holder Handwheel locking Mechanism-lock fixed position Knife holder Handwheel Locking mechanism-lock any position Clamping lever for knife holder Section Waste tray Separate Control Panel Storage Tray Handle of handwheel... -
Page 10: Performance Index
Performance Index AEM460 is a motorized rotary microtome. The stepper motor operated the specimen movement forward and back, coarse feed . It is better in precision sectioning, easy operation. ⊙ The crust is made in ABS plastic with fire prevention function, and streamlined designed in artistic appearance. -
Page 11: 3、Preparing Before Using
⊙ Specimen vertical feed：70mm ⊙ Maximum specimen ：40x50x30mm, or standard cassette ⊙ Specimen holder adjusted system: Horizontal orientation: ±8° Vertical orientation： ±8° ⊙ Repositioning of knife holder base(Left-right):50mm ⊙ Electric Coarse Feed Speed： 300µm/s (Slow) and 900µm/s (Quick) ⊙Dimension: Length：550mm, Width：... -
Page 12: Installation
3.3 Installation 3.3.1 Unpacking As Fig shows： ⊙ Box Cover（1） ⊙ Microtome（2） ⊙ Back protect board（3） ⊙ Section Waste Tray（4） ⊙ Knife holder（5） ⊙ Front protect board（6） ⊙ Specimen Clamp（7） ⊙ Box Base（8） Cut off the tie and then remove the cover (1), and then take knife holder (5), Section waste tray (4), specimen clamp (7), front protect... -
Page 13: Electrical Connection
3.3.4 Separate Control Panel Take the separate control panel（12） and connecting cable（13） out from the box，As Fig shows, insert the plug of the connecting cable into the control panel （ 12） and socket （ 14） on the rear of instrument. And fasten the plug, tighten the two screws. -
Page 14: 4、Operation
4、Operation 4.1 Control Panel Function &Control All the parameters are set and displayed via separate control panel. After power on, use the separate control panel to operate the instrument, the following is the distribution and explanation of separate control panel. 4.1.1 Display Panel LCD display: Indicator Light... - Page 15 If Lock Led lit up，it shows the handwheel is locking and no response to section. CLEAR button Clear the display (section counter or section thickness sum) to 0 ●The system will automatic clear the section number and thickness when machine switch on. 4.1.2 Separate Control Panel Display Area: Trim light: at trimming mode...
- Page 16 Buttons for setting the section thickness / trimming section thickness/ retraction value Section thickness setting range：0µm to 600µm Setting values: From 0µm to 2µm，in 0.5µm increments From 2µm to10µm，in 1µm increments From 10µm to 20µm，in 2µm increments From 20µm to 100µm，in 5µm increments From 100µm to600µm，in 50µm increments Retraction value：From 5µm to 100µm，in 5µm increments.
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Page 17: Knife Holder Inserting
Knife Holder inserting As the drawing shows， the knife holder be composed of the following parts： Z axis slider （1） ，Y slider（2） ，X slider（3） ，clamp2（4） ， clamp1（5） ，knife guard（6）, axis axis lever 2（7）, lever 2（8）, lever 2（9）,lever 1（10）. ⊙ Insert the Y slider （... - Page 18 Knife holder and the instrument base holder Insert the knife holder(11) along the track of the base holder(12), and then rotate lever 2 (7) to lock and adjust the adjustable handle of the lever 2(7) to horizontal position. ▲ Attention: Always remove the knife before detaching the knife holder from the instrument.
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Page 19: Clamping The Specimen
Clamping the Specimen As the drawing shows , turn the handwheel (2) to drive the specimen clamp(1) moving in the vertical direction. ● To clockwise turn the handwheel when sectioning to obtain a best section. drawing shows, specimen clamping system is made up of adjustor(3)、 connect board (4) and clamp(5、6). -
Page 20: Specimen Clamps/Knife Inserting
Specimen Clamps/Knife Inserting Specimen Clamp The specimen Clamp is available two kinds： Specimen clamp （1） and Quick release cassette clamp（2）. Specimen clamp： Put the specimen (4) into the clamp (1) as it showed in the drawing. And then clockwise rotate the knurled screw (3) to tighten the clamp. -
Page 21: Trimming
● Take care when operate the knife holder and the blade. The cutting edge is extremely sharp and can cause serious injury. 4.5 Trimming the specimen ⊙ Retraction setting Active retraction function and set retraction thickness via the button. ，while the retraction Press to light up the LED function be activate, and deactivate by press... -
Page 22: Sectioning
⊙ Memorized coarse feed value and fast reload the next specimen The feature is available for section more quantity cassettes in one time which help to save the reload new specimen trimming time and improve efficiency. The Operations: Clamp one new specimen and lock the knife holder, adjust the specimen to close the cutting edge while press memorize the position and go with buzzer rings. -
Page 23: 5、Clearance & Maintenance
● After sectioning, place the specimen clamping system on the upper position and lock it. Take out the blade and put it in the case box when it is not in use. 5、Clearance & Maintenance 5.1 Cleaning the instrument ⊙ cleaning the appearance Use the wet cloth to clean the areas always be touched when operate the instrument, for example the handle and the base holder locking lever and the storage area on the crust. - Page 24 Rotate the corresponding Levers to split into : Clamp, X-axis slider, Y-axis slider and Z-axis slider Clamp and its X-axis Slider and its Y-axis Slider corresponding lever corresponding lever corresponding lever To obtain a high quality section, it is important to keep cleaning the instrument. So the user must clean the instrument periodically or irregularly according to the total sectioning quantity to obtain the best section ●only authorized and qualified service personnel may access the internal components...
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Page 25: Maintenance
5.2 Maintenance ⊙ Replacing the Fuses ● put the fuse (1) in the installation part (2) and then insert the installation part into the socket(3). ● Cut off the power supply and pull out the plug before change the fuse. Attention: before change the fuse, please read the operation manual carefully. -
Page 26: 6、Trouble Shooting
6、 Trouble Shooting Below you find a list of the problems that most frequently occur. And they are most caused by the operator, so please read the operation manual carefully before use it. Problem Possible causes Corrective action ·Mains cable not properly connected ·Check if mains cable is properly connected ·No display, no reaction or it is break in the circuit. - Page 27 Problem Possible causes Corrective action · The ·Other reasons ·Use an ink brush to smooth the section, and section continuous make the embedded cassette cling with one side of the blade edge, and if this method is effective, it can be applied afterwards and the section connection can be got in most situations.
- Page 28 Problem Possible causes Corrective action ·The section is extremely · · Change the paraffin, and embedded the The wax has been used several times which cause compressed, wrinkled or jammed specimen again the section damaged. together. ·The section is broken or the ·The tissue is not completely ·Dehydrate or clean the tissue again。...
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Page 29: 7、Instrument Diagram
Problem Possible causes Corrective action ·The surface of the section is ·The gradient of blade is not · Re-adjust the gradient of the in wave. proper. blade · The section flies away and ·It is affected by static. ·Increase the surrounding sticks to the microtome or humidity to get rid of the static other objects near the... - Page 30 Standard accessories list AEM460 Accessory Name Notes Microtome 1 unit Blade Holder 1 set Specimen Clamp 1 set Waste Tray 1 pc Separate control panel 1 set With connection cable M3 Allen Wrench 1 pc M4 Allen Wrench 1 pc Disposable blade 1 box Dust Cover...
- Page 31 Published by: AMOS SCIENTIFIC PTY.LTD. 7 Gabrielle Court, Bayswater North VIC 3153 AUSTRALIA Tel: +61-4-10190188 Fax: +61-3-98081503 ABN 37 159 778 140 www.amos-scientific.com Email: info@amos-scientific.com...
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