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Summary of Contents for Amos AST 560
- Page 1 AST 560 Semi-automatic Cryostat Microtome Operation Manual AMOS SCIENTIFIC PTY.LTD. ABN37 159 778 140 www.amos-scientific.com...
- Page 3 Presented in this Operation manual are the structure, functions and using notice. Reading carefully prior to operating the instrument.
- Page 4 Foreword AST560 Cryostat Microtome is a Semi- automatic microtome whose design concept comes from customers all over the world; it can meet different requirements for tissue section. To ensure the instrument works properly, safely and perform durably, it is necessary to read the operation manual carefully.
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Page 5: Table Of Contents
CONTENTS 1、SAFETY NOTES ............................1 1.1O ................................1 VERVIEW 1.2 S ..............................1 AFETY ARNING 1.3 S ..............................2 AFETY EVICE 2、PERFORMANCE & PARAMETERS ....................3 2.1 P & ......................3 RODUCT DESCRIPTION INTENDED USE 2.2 O ......................3 VERVIEW NSTRUMENT OMPONENTS 2.3 P... -
Page 6: 1、Safety Notes
● Don't rotate the handwheel clockwise and counterclockwise frequently at top and bottom , it might lead to deviation of section thickness . ● Ensure no liquid enters into interior of instrument during working . ---1--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 7: Safety Device
▲ Attention: Before removing the instrument , changing specimen/blade or cleaning the instrument ,the handwheel must be locked . The lock indicator is highlighted, indicating the handwheel is locked. Fig 1 Fig 2 ---2--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 8: 2、Performance & Parameters
The main structure of cryostat microtome is shown in Figure 3 as below: Fig 3 Touch Panel Heating glass Left control panel Specimen Clamp Freeze Shelf Blade holder Stationary heat extractor Tool Shelf Peltier on freeze shelf freezing area Lock lever Waste container Handwheel ---3--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 9: Performance Index
2.3 Performance Index AST 560 is a Semi-automatic cryostat microtome whose specimen forward and backward movement is controlled by motor. So it can get high quality section and easily operated. The following is its performance: ⊙The whole instrument follows human engineering theory as well as artistic appearance, made by numerical control machine. -
Page 10: 3、Preparation Before Operating
● Please check the supply carefully with the packing list after opening the carton . If you have any doubt, contact the seller immediately .If you have any special requirement, please illuminate it before the order. ---5--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 11: Installation
Fig 4 Carton Cover Corner Support 3 Upholder Handwheel Corner Support1 Blade Holder Carton body Carton Base Glass Support Corner Support 4 Corner Support 2 Cryostat Microtome ---6--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 12: Electrical Connection
● As fig7 shows power switch . Turn off as the left fig and turn on as right fig. ● After turning on the power , display panel light and the specimen block will be back to the original base position automatically with a beep . ---7--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 13: 4、Operation
6．Freeze shelf temperature Display the freeze shelf temperature 7．Specimen clamp Display specimen clamp temperature ; Click the icon , pop up temperature sub-interface, set its temperature 8． Environment temperature Display the environment temperature ---8--- AST 560 Semi-automatic Cryostat Microtome 201911119... - Page 14 Slow forward/slow backward (single arrow) Adjust the specimen position slowly; the speed could reach300± 30µm/s Fig 9 ● Specimen feed distance is 24mm. Once exceeding this distance, the buzzer will beep and the specimen movement stops. ---9--- AST 560 Semi-automatic Cryostat Microtome 201911119...
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Page 15: Auxiliary Function
Set the selected date and set UV disinfection time. Please note it disinfects disinfection time only when the heating window closed 7Sleep time Set the automatic sleep time 8Back Press the key to return to the main interface after setting the parameters 4.3 Auxiliary Function ---10--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 16: Cryo Chamber Temperature Setting
Notice: Be sure to take out the samples and waste bags etc before entering into defrosting status. The item 6 and 7 is only suitable for samples with similar thickness. 4.4 Cryo chamber temperature setting ---11--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 17: Specimen Clamp Temperature Setting
Click the value beside the specimen clamp icon on main interface. It pops up a sub-interface temperature setting: 1 Temperature Fig 13 It shows current set temperature. The value can be modified by item 2 and 3. setting ---12--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 18: Section, Trimming And Retraction Setting
The thickness range 6.Thickness and refers to section 2.4-Technical parameters. For example, when the thickness Setting range is 0~3μm, the increment is 0.5μm. When you set 2.8μm, the default thickness is 3μm. ---13--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 19: Lock Screen Key & Auxiliary Function Display
⊙Fixed lever 2: It is used to fix the blade holder into the cryo chamber. ⊙Fixed lever 3: Fixed blade clamp can be moved in the direction of arrow B, when it moves to ---14--- AST 560 Semi-automatic Cryostat Microtome 201911119... - Page 20 V-shaped groove (5) at the bottom of the blade holder with the V-shaped groove (6) on rail (1) , and then place the blade holder on the rail (1) gently and push it forward a reasonable ---15--- AST 560 Semi-automatic Cryostat Microtome 201911119...
- Page 21 As fig 19，anti roll plate is rectangular organic glass ,do as follows: ⊙Insert the blade, and rotate the fixed pole (6) to vertical position for tightening it. ⊙Lock the two anti roll plate knobs (11) ---16--- AST 560 Semi-automatic Cryostat Microtome 201911119...
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Page 22: Clamping The Specimen And Inserting The Blade
22.After inserting the blade, refix the anti-roll plate module (8) with the four bolts (7). ● Take care when operating the blade holder and the blade. The cutting edge is extremely sharp and can cause serious injury. ---17--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 23: Section Reference
Detach the blade and put in the box when it is not in use . 5、Cleaning & Maintenance 5.1 Cleaning ⊙ Cleaning the instrument Use the wet cloth to clean the areas always polluted during operation, for example the handle ---18--- AST 560 Semi-automatic Cryostat Microtome 201911119... -
Page 24: Cleaning The Waste Tissue
● Keep cleaning the cryo chamber frequently ! ● Do not turn on the instrument before it is completely dry . 5.2 Cleaning the waste tissue Section extraction is equipped which can conveniently remove the waste tissue produced by ---19--- AST 560 Semi-automatic Cryostat Microtome 201911119... - Page 25 Plug the adaptor with filter bag into the suction hole and keep the two scale line of adaptor (Fig 29) in same level, and fasten it as counterclockwise direction rotation to the end as Fig 28 Fig 28 Fig 29 ---20--- AST 560 Semi-automatic Cryostat Microtome 201911119...
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Page 26: Maintenance
Reconnect the main cable or No display , no reaction to connected or it is break in the circuit replace it. buttons pressed after the instrument is on Mains fuses defective Replace the fuse. ---21--- AST 560 Semi-automatic Cryostat Microtome 201911119... - Page 27 , and the section The gradient of the blade is not proper. are scratched and show the gradient of the blade. vibrated mark If there any other troubles cannot be resolved, please contact with the manufacturer. ---22--- AST 560 Semi-automatic Cryostat Microtome 201911119...
- Page 30 Published by: AMOS SCIENTIFIC PTY.LTD Address: 1 Prestige Dr Clayton South VIC 3169 Melbourne , Australia Tel: +61-4-10190188 Fax: +61-3-98081503 Website: www.amos-scientific.com Email: info@amos-scientific.com...
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