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- Page 1 AST 550 （ Version：AMOSAST550OP20130402） Fully-automatic Cryostat Microtome Operation Manual AMOS SCIENTIFIC PTY.LTD. ABN37 159 778 140 www.amos-scientific.com...
- Page 2 Presented in this Operation manual are the structure , functions and using notice . Reading carefully prior to operating the instrument .
- Page 3 Foreword AST550 Cryostat Microtome is a fully- automatic microtome whose design concept comes from customers all over the world , it can meet different requirements for tissue section . ensure instrument works properly , safely and perform durably , it is necessary to read the operation manual carefully .
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Page 4: Table Of Contents
Contents 1、Safety Notes……………………………………………………...……..1 1.1 Overview……………………………………………………………………..……….1 1.2 Safety Warning………………………………………………………………..….….1 1.3 Safety Device………………………………………………......…...…..….2 2、Performance & Parameters…………………………….......4 2.1 Overview of Instrument Component………………………………….….…...….….4 2.2 Performance Index……………………………………………………………..……..5 2.3 Technical Parameters…………………………………………………………..……..5 3、Preparation before operating………………………………….….…..7 3.1 Requirement of Installation ………………………………………………..……..….7 3.2 Standard Delivery………………………………………………………….....….7 3.3 Installation ………………………………………………………………....….8 3.4 Electrical Connection…………………………………………………....….10 4、Operation ……………………………………………………..….11 4.1 Operate Panel Function &Control…………………………………………..……11 4.2 Installation of Blade Holder…………………………….…………….....….…16... -
Page 5: 1、Safety Notes
1、Safety Notes 1.1Overview The Operator Manual contains important safety instructions and information . In order to operate the instrument safely , the operator must read carefully prior to starting up the instrument . It is very important to ensure safety and avoid instrument damage . Please keep the manual close to the instrument to read easily at any time . -
Page 6: Safety Device
● Ensure no liquid enters into interior of instrument during working . 1.2.3 Warning during cleaning and Maintenance ● Only authorized person may do the service and repair . ● Before cleaning and maintenance of each part, please cut off the power , unplug the power plug , remove the blade holder and blade . - Page 7 Emergency Stop Function Fig (2) is the switch for emergency stop . The emergency-stop function is activated with the red switch (2) pressed while the main power off and all action stops . To deactivate this function , turn the switch in the direction of the arrow .
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Page 8: 2、Performance & Parameters
2、Performance & Parameters 2.1Overview- Instrument Components Display Control Panel Heating Glass Control Panel E-stop switch Stationary Heat Control Panel Extractor Quick Freeze Shelf Specimen Clamp Blade Holder Peltier on freeze shelf freezing Lock Lever Area Waste Container Handwheel Tool Shelf ---4--- AST550 Cryostat Microtome AMOSAST550OP20130402... -
Page 9: Performance Index
2.2Performance Index AST550 is a fully-automatic cryostat microtome whose specimen forward and backward movement are controlled by motor . So it can get high quality section and easily operated . The following is its performance : ⊙ The whole instrument follows human engineering theory as well as artistic appearance , made by numerical control machine . - Page 10 ⊙ Electric coarse feed：Slow 300µm/s±30µm/s Rapid 900µm/s±30µm/s ⊙ Section thickness range： 0 to 100µm 0 to 3µm， in 0.5µm increments 3 to 10µm, in 1µm increments 10 to 20µm, in 2µm increments 20 to 100µm，in 5µm increments ⊙ Trimming thickness range: 10 to 600µm 10 to 50µm，...
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Page 11: 3、Preparation Before Operating
3、Preparation before operating 3.1 Installation site requirement ⊙ The floor stand instrument fixed 4 wheels and 2 fixed support feet . The front only be used during movement . Remove the package and move the instrument to the installation site , then adjust the two fixed support feet and make the front wheels disabled . -
Page 12: Installation
3.3 Installation 3.3.1 Unpacking As showed in the picture，the following is the instrument accessories： ⊙ Carton Cover（1） ⊙ Upholder（2） ⊙ Corner Support1（3） ⊙ Carton body（4） ⊙ Glass Support（5） ⊙ Corner Support 2（6） ⊙ Corner Support 3（7） ⊙ Handwheel（8） ⊙ Blade Holder（9） ⊙... - Page 13 3.3.2 Handwheel assembly ·Take out the handwheel accessories from handwheel box · Tighten the handwheel lever (14) into handwheel (13) with wrench . Insert bearing(15) , handwheel · cover (16) ,bearing (15) handwheel washer (17) in order . · Tighten them with M5 X 16 hexagon socket screw(18) .
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Page 14: Electrical Connection
3.4 Electrical Connection ● The voltage has been preset at the factory . Before connecting the instrument to the power , please check that this setting complies with the local power requirements . ● Connect the instrument to a ground power socket to avoid accident . ●... -
Page 15: 4、Operation
4、Operation 4.1 Control panel function & Control All the parameters are set and displayed via control panel . After power on , use the control panel to operate the instrument as follows: 4.1.1 Display Panel Display area show main information The area is to set the value of Indicator light such as : section thickness , trimming... - Page 16 situation and week time . “ ＋ ” 、 “ － ” The two keys are used for setting section/trimming thickness , time and temperature parameters. Section thickness value : 0 to 100µm 0 to 3µm，in 0.5µm increments 3 to 10µm，in 1µm increments 10 to 20µm，in 2µm increments 20 to 100µm, in 5µm increments Trimming thickness value :10 to 600µm...
- Page 17 is a cycle key to select desired time mode . Short push the button to select: real time , setting of real time , defrosting time , disinfection time , sleep time and wake up time . is the real time indicator light The real time setting is ready when real time light is is defrosting time indicator light The defrosting time setting is ready when the...
- Page 18 is the button to turn on/off lamp . Short push this button to turn on lamp and turn it off by pushing it again . is the button to turn on/off sleep function . Keyboard locking button This key is used to lock the keyboard. Hold on this button until the icon "...
- Page 19 4.1.3 Right control panel Mode selection Run/Stop between manual motorized mode and automatic Speed adjusting button under automatic mode Mode selection between manual and automatic Select the mode by pressing this button shortly. Autorun/ Stop button in motorized mode Press and hold this button about three seconds while the lights into autorun .
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Page 20: Installation Of Blade Holder
4.2 Installation of holder As fig shows , the blade holder is composed of the following parts : blade holder base (1) , longitudinal movable jaw (2) , rotating movable jaw (3) , movable knife clamp(4), fixed knife clamp (5), anti-roll plate (6) , fixed lever 1 (7) , fixed lever 2 (8) , fixed lever 3 (9) , fixed lever 4(10) , anti-roll plate lock knob (11) , anti-roll plate height adjusting knob(12) . - Page 21 Installation of blade holder and base As showed in picture , slide the blade holder(13) along the track(14) in cryostat microtome , rotate the handle of lever 2(8) to horizon position to lock the blade holder . ▲Attention：Before moving the instrument , take out the blade.
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Page 22: Clamping The Specimen&Inserting The Blade
4.3 Clamping the specimen and Inserting the blade Clamping the specimen As showed in the picture , rotate the clamp lever , and insert the specimen(3) into clamp(1) then loosen the clamp lever ● Always clamp the specimen before installing the blade to avoid injury . -
Page 23: Temperature Setting
4.4 Temperature setting 4.4.1 Specimen clamp temperature setting Board area -Press shortly and the line of temperature board area gets thicker . When the display shows , it has entered into specimen clamp temperature display status . -Then press to enter into temperature setting situation . -The value can be adjusted by as showed in left Fig . -
Page 24: Time Setting
4.4.4 Ambient temperature Board area 区域边框 Press shortly and the line of temperature board area gets thicker . When the display shows , it has entered into ambient temperature situation . The time on display is actual ambient temperature . Ambient Temperature 环境温度... - Page 25 4.5.2 Defrosting time setting Press and the line of time board area gets thicker .When the light is on which means the item defrosting time has been selected (as in left Fig) , then press to enter into defrosting time setting . The value can be set through to set minute after hour ；Press...
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Page 26: Section Setting
When the sleep it alone for 5 seconds to exit automatically . function is activated , "Z " icon (shown at left) will be displayed above the time zone. The sleep time can be set separately from Monday to Sunday . 4.5.5 Wake up time setting Press and the line of time board area gets thicker . - Page 27 Trimming thickness ⊙ Trimming setting 修片值 Press SECT/TRIM shortly and the line of trimming board area gets thicker .When the Letter before progress bar becomes "T" which means it has enter into trimming situation , press enter into trimming setting . The section value can be set by .
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Page 28: Retraction Setting
4.7 Retraction setting Press RETRA one time first and the line of retraction Retraction 回缩值 board area gets thicker, then press to enter into retraction setting . The value can be set by . After finishing the setting , press again to exit or let it alone for 5 seconds to exit automatically . -
Page 29: 5、Clearance & Maintenance
side in frozen section . Try to make contact line of tissue and blade shorter . ● After sectioning , place the specimen clamping system on the upper position and lock it . Detach the blade and put in in the box when it is not in use . 5、Cleaning &... -
Page 30: Maintenance
● Only authorized and qualified service personnel may access the internal components of the instrument for cleaning and maintenance . ● Before cleaning and maintenance turn off the instrument , pull out the plug and take down the blade holder and then clean all the parts of instrument separately . The blade must be taken down before cleaning the blade holder . -
Page 31: 6、Trouble Shooting
6、Trouble Shooting Below you find a list of the problems that most frequently occur. And they are mostly caused by the operator, so please read the operation manual carefully before using it . Problem Possible causes Corrective action · No display, no ·... - Page 32 Problem Possible causes Corrective action ·The surface of the · The gradient of the blade is not ·Readjust the gradient . section is in wave . proper . ·Increase the surrounding ·The section flies away ·It is effected by static . humidity o get rid of the static .
- Page 33 Standard accessories list AST550 Accessory Name Notes Cryostat Microtome 1 set Blade Holder 1 set Handling wheel 1 pc Specimen clamp 10 pcs M5 Allen Wrench 1 pc M3Allen Wrench 1 pc 7mm Wrench 1 pc Disposable blade 1 box Power cord 1 pc Brush...
- Page 34 Published by: AMOS SCIENTIFIC PTY.LTD 7 Gabrielle Court,Bayswater North VIC 3153 Melbourne , Australia Tel: +61-410190188 Fax: +61-410190188 ABN 37 159 778 140 www.amos-scientific.com...
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