MRC CRYO-20T User Manual
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MRC CRYO-20T User Manual

Cryoscope
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CRYO-20T
Cryoscope
User's manual
3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529
mrc@mrclab.com
MRC. 2.24

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Summary of Contents for MRC CRYO-20T

  • Page 1 CRYO-20T Cryoscope User’s manual 3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 mrc@mrclab.com MRC. 2.24...

  • Page 2: Table Of Contents

    Cryo-20T Cryoscope User’s manual WARNINGS ..........................7 SYMBOLS USED IN THIS MANUAL ..................7 FEATURES ..........................8 MECHANICAL FEATURES ....................8 ELECTRICAL FEATURES ..................... 8 CLIMATIC FEATURES, STORAGE, PACKAGING AND USE ..........8 STARTER KIT ........................... 9 SAFETY DEVICES ........................9 EMERGENCY PUSHBUTTON ....................
  • Page 3 STARTING ANALYSIS FRAME ................... 22 ANALYSIS FRAME ......................23 SETTING THE NUMBER OF TEST TUBES ................. 25 SETTING THE SAMPLES IDS ..................... 25 A. How to insert IDs manually ................... 25 B. How to insert IDs with a barcode reader ............... 25 SELECTING THE CALIBRATION CURVE ................
  • Page 4 HOW TO DELETE THE ARCHIVES ..................38 A. How to delete the analysis list ..................38 B. How to delete the alarm list ..................39 CONNECTION OF THE CRYOSCOPE TO A PERSONAL COMPUTER ......... 39 BARCODE READER ....................... 40 BARCODES ......................... 40 MENU ..........................
  • Page 5 ORDINARY MAINTANANCE ....................63 CAROUSEL REMOVAL ....................... 63 EQUIPMENT CLEANING ..................... 64 CHECK AND SUBSTITUTION OF THE COOLING LIQUID ..........64 CHECK AND SUBSTITUTION OF THE FILTER ..............66 HOW TO CLEAN THE FILTER .................... 67 DAILY MAINTENANCE ......................68 EXTRAORDINARY MAINTENANCE ..................
  • Page 6 C. Spare parts ........................80 WARRANTY ..........................81 DISPOSAL ..........................81 COOLING BATH FORM ......................82 DECLARATION OF CONFORMITY ..................83...

  • Page 7: Warnings

    WARNINGS Carefully read this User Guide before using the equipment. This manual is an integral part of the product and must be conserved for future consultations. Here, you can find important information regarding safety of use and maintenance. Read carefully the instructions contained in this manual before installation, use and before processing any operation of adjustment or maintenance.

  • Page 8: Features

    Presence of danger: injuries may occur. Please follow the instructions contained in the related paragraph. Presence of danger: electrical shock may occur. Please follow the instructions contained in the related paragraph. Crush hazard. This symbol is located on the head of the equipment and warns the user about the risk of injury.

  • Page 9: Starter Kit

    STARTER KIT CRYO-20T is supplied with a Starter Kit containing Code Description Quantity 08103 Filter for liquid MLL67290 Spring EP-50 Thermal printer 67219 Paper roll for printer 67233 Cable for printer (Basic, Smart, Style, Touch) 63215 Calibration Standard -0.408°C, 250 mL 63220 Check Standard -0.512°C, 250 mL...

  • Page 10: Electrical Connection

    ELECTRICAL CONNECTION Avoid placing the equipment near heating sources or windows hit by sunlight. Place the instrument far from the wall: in this way it would be easier to operate both on the main switch and on the socket. The equipment works with 230 V ~ ±10% and a frequency of 50 Hz or with 115 V ~ ±10% and a frequency of 60 Hz.
  • Page 11 6. Turn the cryoscope on by pressing the main switch positioned on the back. 7. If the equipment does not turn on, verify that the emergency pushbutton is not pressed. In this case deactivate it by turning it in the direction indicated on the head and try to turn it on again.
  • Page 12   Pay attention not to hit the reading thermistor! 10. Turn the equipment off. 11. Fill the vessel positioned on the back of the equipment with cooling liquid until the maximum level indication is reached.     ...
  • Page 13 Check that the little pipe collects from the very bottom of the vessel and the waste tube is positioned over the “maximum level” indication. The wrong position of the pipes may generate errors during the analysis. 12. Turn the instrument on again: the cooling bath will be filled automatically. 13.

  • Page 14: Main Menu

    The working condition of the equipment indicates whether the cryoscope is ready to start the analysis or not (i.e. it may be necessary to wait that the cooling bath reaches the working temperature). 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 Cal.: ISO Type: Cow T.
  • Page 15 Frame Information/warning 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 Button (Tool) bar Main Frame Cal.: ISO Type: Cow T. Bath -88.8 °C WAIT... Entry Description It appears when an alarm is activated. Alarm See paragraph “Alarms” to get more information.
  • Page 16 See paragraph “Transferring the data on the pen drive” to get more information. It shows the name of the customer. Customer Model It shows the model of the cryoscope. CRYO-20T It shows the current date. Date 28 / 09 / 2018 It shows the current time. Time...
  • Page 17 Information It enters the “Information menu”. It allows the operator to set the reference value for the added H2O Reference water calculation. It enters the calibration function. Calibration It allows the operator to lift the head manually. Head Up It allows the operator to lower the head manually. Head Down It allows the operator to rotate the carousel manually.

  • Page 18: Keyboard

    Cryo offers both numeric and alphanumeric keyboards A. Numeric Keyboard The equipment shows the numeric keyboard when the customer needs to insert a number. 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 HIGHER POINT CURRENT VALUE - 408 m°C ...

  • Page 19: Alphanumeric Keyboard

    It inserts the new value. Insert key B.Alphanumeric Keyboard The equipment shows the numeric keyboard when the customer needs to insert digits and numbers. 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 SAMPLE ID Standard  ...

  • Page 20: Password

    Entry Description Parameter Name Name of the parameter to be modified. ID Sample Current value Inserted data. Standard Insertion key. Previous It goes back to the previous menu. It goes to the homepage. Home PASSWORD The operator can protect the setting functions by a password Please note that not all the Cryo functions are password-protected: some of them have free access (i.e.
  • Page 21 In order to deactivate the restriction: 1. Press the symbol 2. Insert the password by using the alphanumerical keyboard (see “Keyboard” paragraph). 3. After the confirmation the display will return to the previous frame and the symbol will appear in the Information/warning bar. In order to activate the restriction: 1.

  • Page 22: Analysis And Calibration

    IDs (see “How to insert ID samples” paragraph) and start the analysis by using “Milk” mode (see “Analysis of milk samples” paragraph) or “Standard” mode (see “Analysis of standard / calibration liquids” paragraph). 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 ID Sample ID Sample N.01 ID Sample N.02...

  • Page 23: Analysis Frame

    It goes to the homepage. ID Sample If pressed, it allows the operator to digit the sample ID. ID Sample N.XX ANALYSIS FRAME 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 Last Analysis SampleN. 02 Crioscopy -534.0 m°C Water 0.00 %...
  • Page 24 Entry Description Diagram It shows the temperature diagram of the current analysis. Stop It stops the current analysis. Number It shows the number of current analysis. N. 1 It shows the sample ID number related to the current analysis. It shows the set reference value. Reference Ref.

  • Page 25: Setting The Number Of Test Tubes

    SETTING THE NUMBER OF TEST TUBES In order to reduce the time it is possible to set the number of test tubes to be analyzed before starting the run. To set the number of test tubes, proceed as follows: 1. Enter the menu by pressing the key.

  • Page 26: Selecting The Calibration Curve

    SELECTING THE CALIBRATION CURVE The equipment is provided with two calibration curves named ISO and Lactose-Free. The operator can select the calibration curve according to the kind of analysis to be performed by following these steps: 1. Press the key from the main frame. 2.

  • Page 27: Analysis Of Standard / Calibration Liquids

    ANALYSIS OF STANDARD / CALIBRATION LIQUIDS The results obtained after performing the analysis under this mode do not take into consideration the set reference value, therefore the percentage of added water is not calculated. The saved data will be shown in BLACK. 1.

  • Page 28: Analysis Of Milk Samples

    The results obtained from the analysis are stored in the internal memory of the cryoscope. The operator can consult these results by pressing the key and press Cryoscopy List. ANALYSIS OF MILK SAMPLES The results obtained after performing the analysis under this mode take into consideration the set reference value, therefore the percentage of added water is calculated.

  • Page 29: Rapid Start

    11. After finishing the analysis of the samples, the display returns to the main frame, the carousel stops and the pump refills automatically. 12. Remove the test tubes from the carousel. The results obtained from the analysis are stored in the internal memory of the cryoscope.

  • Page 30: Calibration

    In case the repeatability is higher than ±2.0 m°C if compared to the standard value, check the amplitude of both agitation and freezing stroke. If the problem persists, contact your local MRC Distributor It is possible to perform the calibration by using a different number of replicates for each standard (the number of replicates indicated in this manual must be intended as a minimum number).

  • Page 31: Standard Calibration By Using -600.0 M°C

    If the repeatability value is not higher than ±2.0 m°C it is possible to confirm the value; otherwise stop the calibration process and check both the agitation amplitude and freezing stroke instead. If the problem persists, contact your local MRC Distributor 13. Press the key to confirm.

  • Page 32: Check The Calibration By Using -512.0 M°C Standard

    - Whether the accuracy results to be out of the range of ±3.0 m°C it is necessary to verify the quality and expiry date of the standard liquids and/or repeat the calibration process. If you get wrong results after performing these remedial actions, contact your local MRC Distributor...

  • Page 33: Calibration Of Lactose-Free Curve

    If the repeatability value is not higher than ±2.0 m°C you can confirm the value; otherwise stop the calibration process and check both the agitation amplitude and freezing stroke instead. If the problem persists, contact your local MRC Distributor 13. Press the key to confirm the value.

  • Page 34: Calibration By Using The Lower Standard

    If the repeatability value is not higher than ±2.0 m°C you can confirm the value; otherwise stop the calibration process and check both the agitation amplitude and freezing stroke instead. If the problem persists, contact your local MRC Distributor 29. Press the key to confirm the value.

  • Page 35: Linearity Adjustment

    If you get wrong results after performing these remedial actions, contact your local MRC Distributor LINEARITY ADJUSTMENT The function of Linearity Adjustment is strictly bounded to the calibration of the equipment.

  • Page 36: Handling The Archives

    4. Press the key. 5. Press the key. 6. The display shows this message. CONFIRM SETTING?    7. Press the key to confirm. 8. Press the key to return to the homepage. 9. Verify the new setting as indicated in the “C. Checking the calibration by using -512.0 m°C standard”...

  • Page 37: Connecting The Pen Drive

    A. Connecting the pen drive To download the data on the pen drive, connect it to the cryoscope by means of the PEN DRIVE port located on the right side of the cryoscope. If the pen drive is connected properly, the symbol appears on the Information/warning bar (see “Main Menu”...

  • Page 38: Downloading The Alarm List On The Pen Drive

    C. Downloading the alarm list on the pen drive To download the alarm list from the cryoscope to the pen drive proceed as follows: 1. Verify that the pen drive is properly connected (see “Connecting the pen drive” paragraph”). 2. In case the password is activated, press the key and insert the password (see “Password”...

  • Page 39: How To Delete The Alarm List

    B. How to delete the alarm list To delete the alarm list proceed as follows: 1. In case the password is activated, press the key and insert the password (see “Password” paragraph). 2. Press the key from the Main Menu. 3.

  • Page 40: Barcode Reader

    BARCODE READER Cryo can be connected to a barcode reader by using the rear port located on the SCANNER To get more information about the connection operation, refer to the reader installation manual. BARCODES The equipment can scan barcodes of maximum 13 digits length. Herewith below there are 4 types of barcodes that can be used for the calibration standards and to verify the correct functioning or the correct setting of the reader.

  • Page 41: Menu

    INFORMATION MENU The menu contains the list of all the analysis performed, the list of alarms generated by the equipment and all the main information. 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 INFORMATION Cryoscopy List Alarm List...
  • Page 42 Entry Description Cryoscopy List It displays the complete list of the cryoscopies performed (see “Cryoscopy List” paragraph). It displays the complete list of errors generated by the equipment. Alarm List (see “Alarm List” paragraph). Sensors Signal It shows the real time value for the sensors located inside the instrument.

  • Page 43: Setting Menu

    SETTING MENU This menu contains all the setting entries of every parameter. 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 SETTINGS Language Test Tubes Nr. Unit Selection Celsius Measure Method Tempo H2O Formula (Ris-Rif)*0.18 Agitation Customer Name...
  • Page 44 Measure Method It selects the measure method. The final result obtained will correspond to the chosen method: - Plateau-ISO: the result obtained is the temperature read by the thermistor when the variation is lower than 0.5 m°C for 20 seconds. - Tempo: the result obtained is the temperature read after an established time starting from the freezing stroke.

  • Page 45: Milk Selection Menu

    The Milk Selection Menu allows the user to change the kind of milk in order to select the reference value for the calculation of added water rapidly. 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 TYPE OF MILK ...
  • Page 46 Entry Description Type of milk Kind of milk to be selected. Sheep Goat Buffalo Lactose-free Other Edit Reference It shows and modifies the reference values set. Edit Reference This symbol indicates the kind of milk activated as reference Selected value. The kind of milk is also indicated on the main page so that the ...

  • Page 47: Calibration Thermistor Menu

    CALIBRATION THERMISTOR MENU The calibration menu selects the calibration curve, acquires the analysis, modifies the linearity of the calibration curve and resets the calibration. 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 CALIBRATION THERMISTOR Cal.: Lactose-free Last analysis: -410.0 m°C...
  • Page 48 Confirm lower standard It registers the value of the last analysis as calibration point. Confirm standard -600.0 m°C It modifies the linearity of the calibration curve (see Modify Linearity “Linearity adjustment” paragraph). It resets the calibration; then a new calibration is necessary Reset calibration (see “Calibration”...

  • Page 49: Cryoscopy List Menu

    It is possible to transfer the data on the software (if installed on the computer), transfer them on the pen drive or cancel all the analysis performed. 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 CRYOSCOPY LIST...

  • Page 50: Analysis Details

    It downloads the analysis results on the pen drive (if connected). It erases the archives of the analysis performed (see “How to Cancel delete the archives” paragraph). It scrolls the analysis on different pages. Scroll It returns to the homepage. Home A.
  • Page 51 ID number of the analysis (see “Setting the samples ID” paragraph). STD512-63220 ID number of the current analysis. It indicates the position on the carousel where the sample has been inserted. It indicates the result and the measurement unit Analysis According to the analysis mode selected the result will be: - Standard: the result appears in BLACK (see “Analysis of -513.0...

  • Page 52: Alarm List Menu

    It is possible to transfer the data on the software (if installed on the computer), transfer them on the pen drive or cancel all the analysis performed. 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 ALARM LIST...

  • Page 53: Alarm Detail

    Cancel It erases the archives of the alarms generated (see “How to delete the archives” paragraph). It shows the error messages on different pages. Scroll Home It returns to the homepage. A. Alarm detail All the results of the analysis are saved as follows: DATE Alarm 01/08/18...

  • Page 54: Agitation Menu

    AGITATION MENU The agitation menu contains the agitation adjustment and the test key for the agitation test and freezing stroke test. 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 Agitation Agitation extent 47 % Entry Description Parameter name Name of the parameter.

  • Page 55: Alarms

    Scroll It scrolls the menu entries (function disabled). It returns to the previous menu. Previous Home It returns to the homepage. ALARMS In case of danger or malfunctioning the equipment generates alarms. ALARM WARNING AND CANCELLATION When an alarm is activated the display shows the icon and makes an audible alarm signal too.
  • Page 56 28 / 09 / 2018 CRYO-20T 16 : 32 : 46 ACTIVE ALARMS Bath Alarm Entry Description This symbol indicates that an alarm is activated. Alarm Active Alarm It indicates the active alarm (see “Alarm List” paragraph). Bath Alarm Alarm Cancellation It cancels the current alarm.

  • Page 57: Alarm List

    Home It returns to the homepage. ALARM LIST Entry Description Head Motor Timeout The head is taking too much time to reach its upper / lower position. This alarm indicates that something is blocking the raise/fall of the head or that the motor is not working properly. Cryoscopy Timeout equipment cannot reach...

  • Page 58: Cooling Liquid Error Message

    (- 7.0°C) and then reaches a temperature of more than 3.0°C. If the problem persists, contact your local MRC Distributor COOLING LIQUID ERROR MESSAGE After the analysis of 200 samples or after 93 working days the cooling liquid alarm is generated automatically.

  • Page 59: Check Test Tube" Message

    2. The test tube is attached to the mandrel and comes out from the carousel. 3. The test tube broke during the analysis. In case one of these problems occurs, contact your local MRC Distributor Press to erase the error message.

  • Page 60: Stirrer Adjustment

    STIRRER ADJUSTMENT In case some problems of bad agitation adjustment or bad freezing stroke adjustment occur during the analysis, it is possible to test these parameters and make a further adjustment if necessary. AGITATION The liquid should be shaken energetically, but no bubbles must be formed during the agitation test process.

  • Page 61: Agitation Adjustments

    B. Agitation Adjustments In order to adjust the agitation amplitude proceed as follows: 1. From the main frame press the key. 2. Press the entry Agitation. 3. Insert the password (see “Password” paragraph). 4. Press the entry Agitation amplitude. 5. Insert the value by using the numeric keyboard. ...

  • Page 62: How To Protect The Thermistor

    The adjustment is a technical operation and must be carried out only if the stirrer does not function properly. 1. Rotate the locking system anticlockwise. 2. Increase or decrease the stirrer strength by rotating in a clockwise or anticlockwise direction. 3.

  • Page 63: Ordinary Maintanance

    ORDINARY MAINTANANCE CAROUSEL REMOVAL Always remove the carousel before cleaning the equipment by following the instructions below: 1. Unscrew and remove the valve, which fixes the carousel to the equipment. 2. Remove the carousel. 3. Remove the plexiglass ring.  ...

  • Page 64: Equipment Cleaning

    EQUIPMENT CLEANING Before starting the cleaning process, always turn the equipment off and unplug it from the electrical connection. Do not pour water on the equipment, but use a dump cloth instead. Clean the equipment with a neutral detergent. Do not use alcohol or aggressive detergents. CHECK AND SUBSTITUTION OF THE COOLING LIQUID Check the level and the clearness of the liquid every day.
  • Page 65       ...

  • Page 66: Check And Substitution Of The Filter

    CHECK AND SUBSTITUTION OF THE FILTER Check the filter condition every day. The filter cleaning is necessary when: 1. The message: “Subst. Cooling Liquid” appears on the display. 2. The filter is very dirty. Replace the filter after having cleaned it up for a few times. In order to replace the filter please proceed as follows: 1.

  • Page 67: How To Clean The Filter

      HOW TO CLEAN THE FILTER In order to clean the filter please proceed as follows: 1. Disconnect it from the pipe that connects it to the aspiration pump. 2. Let warm water flow through the filter in the opposite direction than the one indicated by the arrow.

  • Page 68: Daily Maintenance

     No. Description Syringe Filter DAILY MAINTENANCE 1. Dry the wet parts thoroughly, absorbing all the cooling liquid (we recommend to remove the carousel for a better cleaning). 2. Carefully clean and dry the mandrel. 3. Check and restore the cooling liquid level, if necessary 4.

  • Page 69: Extraordinary Maintenance

    EXTRAORDINARY MAINTENANCE We warmly suggest to subscribe a yearly maintenance agreement with our local Service Center for a complete cleaning operation to the heat exchangers and a general check of the good conditions of the electric and electronic parts. In this way, you can prevent serious damages to the equipment. Every maintenance operation, which is not mentioned in this manual, must be carried out by qualified personnel who has been authorized by the manufacturer only.

  • Page 70: How To Move And Ship The Cryoscope

    HOW TO MOVE AND SHIP THE CRYOSCOPE MOVING THE CRYOSCOPE Every time you need to move the instrument in your laboratory, pay attention to keep it in horizontal position to avoid a liquid loss. Always hold the equipment by its sides and its lower part with the help of another person.
  • Page 71   CAUTION! Should you send the equipment, the use of original MRC package or a strong box filled with styrofoam is mandatory. The use of a pallet would be strongly appreciated.

  • Page 72: Main Spareparts

    MAIN SPAREPARTS COOLING BATH The bath is cooled by means of Peltier cells at a temperature of -7°C ±0.5°C. HEAD The head holds the sample–tube mandrel along with the reading thermistor and the stirrer. Description Reading thermistor Stirrer WELL The tube must be allocated inside the well. When descending the mandrel, the tube will be pushed inside the duct.

  • Page 73: Reading Thermistor

    READING THERMISTOR This is the most fragile part of the equipment: protect the glass from hits or it may be damaged. It must be correctly positioned in order to keep the temperature sensor at the same precise distance from the walls and from the bottom of the test tube. STIRRER The stirrer must be positioned parallel to the thermistor holder keeping a distance of 1 mm.

  • Page 74: Photocell

    The spring must be positioned at the same level of the conduct. If the spring is higher than the conduct, it can be dragged outside by the tubes. If the spring position is too low, with relation to the conduct level, the tube could obstruct the rotation of the carousel plate.

  • Page 75: Rear Panel

    REAR PANEL No. Description No. Description Automatic refilling tube. Cooling liquid tank. Barcode reader serial port. Exhaust tube. Trimmer. Main switch. Filter. Fuses compartment. Printer serial port. Supply socket. Computer serial port. Pump.

  • Page 76: Drain

    DRAIN The drain is located on the left part of the equipment and its purpose is to drain the DRAIN exceeding liquid which is present on the plate. The drain is a safety device and can be useful if the customer does not clean the equipment properly, according to the cleaning procedure described in the manual PRINTER 24/07/18 - 11 : 01 : 15...

  • Page 77: Printer Connection

    Entry Description It indicates the date of the analysis performed. Date 24/07/18 Time It indicates the time of the analysis performed. 11 : 01 : 15 Serial Nr. It indicates the serial number of the equipment. N. SERIE 888888888 It indicates the ID number of the sample. ID Sample ID: 8888888888888 Calibration Curve...

  • Page 78: Paper Feeding

           B. Paper feeding In order to scroll the paper, press the key located located on the right side of the front panel of the printer. C. Change the paper roll In order to change the paper roll proceed as follows: 1.

  • Page 79: Accessories, Consumables And Main Spare Parts

          ACCESSORIES, CONSUMABLES AND MAIN SPARE PARTS A. Accessories Code Description Quantity NPX2-10000 Automatic pipette, 1 - 10 mL volume BMT2-X Tips for automatic pipette, 200 pcs. 67241 Tube holder in stainless steel, 50 places 67251 24-place ABS plastic tube holder...

  • Page 80: Consumables

    9052101006 Stirrer 67248 Reading Thermistor MRC reserves the right to review and modify this document without any notice. Please note that any variation will not affect the respect of the directives. The images used in this manual must be considered as examples and may...

  • Page 81: Warranty

    (tubes, cooling liquid, calibration and check standards) not supplied by MRC or not original replacement parts. Warranty will be instantly invalidated in the event that the serial number of the product is found to be modified or cancelled.

  • Page 82: Cooling Bath Form

    NOTE: this form is an essential part of the bath and must always be kept with it. If returned for any damage without this form, the repairs and the replacement under warranty conditions will not be possible. In this case the manufacturer reserves the right to apply the warranty conditions or not. MRC's Technician ___________________...

  • Page 83: Declaration Of Conformity

    DECLARATION OF CONFORMITY Product name: CRYO-20T Type of product: Cryoscope Manufacturer: MRC Ltd Address: 3, Hagavish st. Israel 58817 , mrc@mrclab.com The above-mentioned equipment is in compliance with the EU harmonized :standards UE/2014/35 UE/2014/30 2011/65/UE By using the following harmonized norms:...

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