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4
Typhoon Operating Instructions 28-9623-56 AA
d)
The figure below shows a top view of instrument with instrument front (A) and
glass platen (B).
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A1
Load the Storage phosphor screen (C) on the instrument glass platen (B) with
the white, phosphor side of the screen face down in the lower left corner (A1).
Each lettered or numbered grid segment is marked by two dots.
e)
Gently lower the screen on the glass platen.
Note:
An unmounted screen may not lie completely flat against the glass.
When you initiate the scan, the instrument lowers the inner section of
the lid to hold the screen flat.
If performing fluorescent or chemiluminescent scanning:
To place a gel or a membrane sample in the instrument.
For information how to scan several samples simultaneously and how to scan
sandwich gels and microarrays, please refer to the Typhoon User´s Guide.
Note: Make sure you place the sample in the correct position when you first set it
down. Any fluorescent or chemiluminescent material left on the glass platen
when you move the sample can result in a double image.
Before you load a sample, determine how to position it on the glass platen:
a)
Sample orientation.
Determine whether to place the sample face up or face down on the glass
platen. The Typhoon system illuminates the sample and collects data from
underneath the sample. Make a note of the orientation of the sample.
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For a one-sided, opaque sample (such as a membrane or TLC plate), place
the sample face down.
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Operation 4
Performing a scanning 4.4
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