Thermo Scientific 1300 Series Operating Manual
Class ii, type a2 biological safety cabinet
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- User manual (130 pages) ,
- Operating manual (76 pages) ,
- Installation manual (10 pages)
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Summary of Contents for Thermo Scientific 1300 Series
- Page 1 1300 Series A2 Class II, Type A2 Biological Safety Cabinet Operating Manual 7011355 Rev. 10...
- Page 2 This user’s manual provides instructions on how to use the 1300 Series A2 most effectively and safely. The 1300 Series offers a unique range of product features that will enhance your safety and improve overall operating efficiency. Should you have any questions on using this product or need further explanation of any of it’s features, please...
- Page 3 Thermo Scientific makes no representations or warranties with respect to this manual. In no event shall Thermo Scientific be held liable for any damages, direct or incidental, arising out of or related to the use of this manual.
- Page 4 EU Member State European Country, and this product should be disposed of or recycled through them. Further information on Thermo Scientific’s compliance with this directive, the recyclers in your country and information on Thermo Scientific products will be available at www.thermo.com.
- Page 5 We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products. Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service...
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Page 6: Table Of Contents
Unit Disposal ......... . .4-8 Thermo Scientific 1300 Series A2... - Page 7 Data Log/Warranty ......... . .9-1 1300 Series A2...
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Page 8: Description
The optional, factory-installed UV light is installed in the ceiling at the front of the sample chamber. Two service valve access ports are located on each side panel. When installing service valves, remove the center of the port plugs at the perforations. Thermo Scientific 1300 Series A2... -
Page 9: Safety Systems
HEPA filters to remove contaminants so that the samples are always surrounded by ultra-pure air. Harmful particles are not carried over the sample chamber (protection from cross-contamination). HEPA filters The downflow air within the chamber and the exhaust air are cleaned by HEPA filters. 1300 Series A2 Thermo Scientific... - Page 10 LEDs. As the unit ages with use, the colors change. Red-red is unacceptable and the unit should not be used. Any questions as to the safety of the unit should be resolved before use. Thermo Scientific 1300 Series A2...
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Page 11: Hepa Filters
These grids prevent large items such as paper towels and tissues from entering the plenum where they may impair the function of the blowers or the filters. The grids can be easily removed for cleaning. 1300 Series A2 Thermo Scientific... -
Page 12: Control Panel
• remaining time of the UV disinfection routine The keys turn functions on or off. Status indicators show: • window work position • airflow • reduced blower speed • Thermo Performance Factor Thermo Scientific 1300 Series A2... -
Page 13: Use Of The Window
Note The working and loading window positions are indicated on the user interface, as controlled by micro switches and displayed by status indicators on the control panel. Window 1300 SERIES A2 Max opening for loading samples Work position... - Page 14 UV light will not function. dimple Figure 1-5. 10 inch Opening (Work Position) Figure 1-6. Lighted Green LED dimple Figure 1-7. Fully Closed Position (UV, if applicable) Figure 1-8. Lighted Blue LED Thermo Scientific 1300 Series A2...
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Page 15: Unit Interface
Warning If a gas burner is to be operated in the sample chamber, an appropriate shut-off valve for the gas supply must be installed. Use only laboratory safe burners in the sample chamber. 1300 Series A2 Thermo Scientific... -
Page 16: Chamber Lighting
Service valve Figure 1-9. Interfaces Chamber Lighting Lighting for the sample chamber includes one fluorescent bulb for 4 foot units, and two bulbs for 6 foot. The bulb(s) are installed behind the light canopy. Thermo Scientific 1300 Series A2... -
Page 17: Uv Lights
To attach the armrests, insert into the first perforated track of the work tray. Warning Work safety is assured only if the armrests are used correctly! Work tray Perforation track Width Armrest Separation Depth distance Perforation Armrest track Figure 1-11. Work tray 1-10 1300 Series A2 Thermo Scientific... -
Page 18: Installation
When positioning the cabinet, make sure the counterweight on the back of the unit can move freely. The minimal distance to the wall or adjacent objects should be 3 inches (7.6cm) Thermo Scientific 1300 Series A2... -
Page 19: Optional Exhaust Transition
Exhaust opening 3. Apply sealant onto the contact Threaded surfaces. inserts 4. Secure the accessory by tightening the supplied retaining screws (M 5) wrench-tight. 5. Remove any excess sealant. Figure 2-1. 4 ft Thimble 1300 Series A2 Thermo Scientific... - Page 20 Apply sealant to the cover, place the Pipe cover onto the connecting Sleeve opening at the housing and Cover secure it with the screws. Sleeve Opening Figure 2-2. 6 ft Thimble and Sleeve Thermo Scientific 1300 Series A2...
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Page 21: Moving The Unit
Warning The weight of the window is balanced by the counterweight on the back of the cabinet. Do not move the unit unless the counterweight has been locked in place with the shipping screws (see Figure 3-2). Figure 2-1. Lift points 1300 Series A2 Thermo Scientific... -
Page 22: Service Valve Connections
7. Secure the service valve to the side panel using the nut. 8. Establish the connection to the supply line using a union nut. Note If a service valve is removed, the service valve port plug should be re- installed. Thermo Scientific 1300 Series A2... - Page 23 Section 2 Installation Valve Connections (continued) Service valve Service valve port plugs Washer Bezel Threaded pipe Figure 2-4. Valve Assembly and Installation 1300 Series A2 Thermo Scientific...
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Page 24: Universal Piping Connections
Pipe threads are 1/4” FPT. Refer to illustration below. top access points right side left side access points access points bottom of cabinet access points bottom of cabinet access points Figure 2-5. Possible Universal Piping Access Points Thermo Scientific 1300 Series A2... -
Page 26: Start-Up
(680 - 880mm ). To set the stand to the desired height, pull the legs downward out of the channels to the desired height. Lock the height adjustment in place by installing two screws per channel to the leg. Thermo Scientific 1300 Series A2... - Page 27 6. Place the safety cabinet onto the stand so that the Allen screws are routed through the holes of the keyhole tabs. 7. Slide the cabinet frame to the keyhole stop (Fig. 3-1). 8. Tighten the four Allen screws. 9. Level the cabinet (instructions follow in this section). 1300 Series A2 Thermo Scientific...
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Page 28: Unlock Counterweight
(feet) of the stand using an open end or adjustable wrench until the level indicates an absolutely horizontal position in all directions. Check from right to left, and from back to front. Thermo Scientific 1300 Series A2... -
Page 29: Power Connection
On connection to the power source, place the window at the working position and wait until all lights on the display are green. The cabinet is now ready for operation and can be operated using the control panel. 1300 Series A2 Thermo Scientific... -
Page 30: Installation Tests
Caution The initial operation with subsequent installation test does not include any decontamination measures. The sample chamber and any accessories required must be disinfected and cleaned in accordance with the hygiene guidelines set forth for the desired application. Thermo Scientific 1300 Series A2... -
Page 31: Locating A Certifier
PO Box 130140 789 N. Dixboro Rd Ann Arbor, MI 48113-0140 Telephone (734) 769-8010 Or (800) NSF-MARK Fax (734) 769-0109 http://www.nsf.org/Certified/Biohazard-Certifier IAFCA PO Box 12155 Columbus, OH 43212 Telephone (888) 679-1904 Fax (614) 486-1108 http://www.iafca.com/certifier.html 1300 Series A2 Thermo Scientific... - Page 32 Telephone (919) 787-5181 Fax (919) 787-4916 http://www.cetainternational.org/members/corp_indiv.htm Note Unless certification was expressly called for in the specification, quotes and/or purchase order, the cost for this on-site testing is to be paid for by the customer. Thermo Scientific 1300 Series A2...
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Page 34: Operation
• with UV light (optional) timer on, the remaining disinfection time. Press the Hours/Velocities key to switch between the operating hours, and the downflow and exhaust air velocities in safe work mode (with window in the open, safe operating work position). Thermo Scientific 1300 Series A2... -
Page 35: Unit Start-Up
The side guide rails have markings that define the lower edge of the front window. These markings can be used as an additional orientation aid. 3. Wait until the green LED “airflow is steady” illuminates. 4. The unit is ready for operation. 1300 Series A2 Thermo Scientific... - Page 36 LED “airflow reduced” are lighted. The sample chamber illumination is enabled. The internal power supply in the sample chamber is enabled. The red LED “airflow not steady” and the blue LED “internal power supply activated” are lighted. Thermo Scientific 1300 Series A2...
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Page 37: Operating Modes
4. Load the work tray with samples. 5. For work breaks or for extended experimental phases without manual intervention, switch the device to standby mode by closing the window. 1300 Series A2 Thermo Scientific... -
Page 38: Error Messages
• Do not place accessories into the sample chamber that cause air turbulence or emit excessive heat. • Do not block air circulation at the ventilation slots of the work tray. Thermo Scientific 1300 Series A2... - Page 39 After completing a procedure, 1. Remove samples from the sample chamber and store them properly. 2. Clean and disinfect the sample chamber surfaces, including the work tray and the drain pan. 3. Clean and disinfect all accessories. 1300 Series A2 Thermo Scientific...
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Page 40: Procedure Interrupt
Warning As this unit can be used for processing and treating infectious substances, it must be decontaminated prior to disposal, in accordance with acceptable standards and procedures. Thermo Scientific 1300 Series A2... -
Page 42: Cleaning / Decontamination
When using a disinfectant with an alcohol content of more than 70 %, the release limit of 200 g (7 oz) within 2 hours must not be exceeded. Also appropriate are disinfectants based on quaternary ammonium compounds. Thermo Scientific 1300 Series A2... -
Page 43: Pre-Disinfection
Final Disinfection 1. Spray disinfectant on all chamber surfaces again, or wipe the surfaces clean with disinfectant. 2. Allow disinfectant to remain as recommended by the manufacturer of the disinfectant. 1300 Series A2 Thermo Scientific... -
Page 44: Uv Disinfection After Wipe/Spray Disinfection
30 minutes. 5. To save the value, press the UV key. The display shows the operating hours again. Note The UV disinfection time is adjustable from 0:00 (disabled) to 24:00 hours. Thermo Scientific 1300 Series A2... -
Page 45: Disinfection With Formaldehyde
4. Remove the cleaning liquid from the drain pan and dry the surfaces thoroughly. Note After cleaning, make sure that all cleaning product has been removed completely from the drain pan. 5. Re-install the work tray. 1300 Series A2 Thermo Scientific... -
Page 46: Clean The Paper Catch Grid
Caution Do not operate the unit without the paper catch grids installed! Retaining Grid section Grid section Inner back wall Retaining Figure 5-1. Protective Paper Catch Grid Thermo Scientific 1300 Series A2... -
Page 48: Maintenance
Warning The diffuser plate on the chamber ceiling serves as protection for the downflow filter and prevents refluxing of downflow air. When scanning the filter surface for a leak test, the perforated plate must be removed. Thermo Scientific 1300 Series A2... -
Page 49: Service
The UV bulb is installed in rotating sockets. To remove, rotate the bulb counterclockwise to disengage the latch and remove it from the sockets. 4. To install, slide the bulb contact pins into the rotating socket grooves and rotate the bulb clockwise to latch the sockets. 1300 Series A2 Thermo Scientific... -
Page 50: Replacements And Repairs
Warning As this unit can be used for processing and treating infectious substances, it may become contaminated. Prior to disposal, the entire unit with filters must be decontaminated in accordance with acceptable standards and procedures. Thermo Scientific 1300 Series A2... -
Page 52: Specifications
/ in 23 / 0.91 Distance lower edge mm / in 150 / 5.91 Distance from back panel First access port mm / in 150 / 5.91 Second access port mm / in 250 / 9.84 Thermo Scientific 1300 Series A2... - Page 53 °K < 2 < 2 Ergonomics Noise level* dB(A) 63, ±2 65, ±2 *The noise level was determined in accordance with NSF/ANSI 49. 2004. The measurement uncertainty is within a range of ± 2 dB. 1300 Series A2 Thermo Scientific...
- Page 54 Power input maximum 1130 / 1700 1440 / 2000 Protection Protection class Protection type IP 20 Overvoltage category (IEC 61010-1, UL61010-1) Contamination degree (IEC 61010-1, UL61010-1) Connecting lines Mains connection Power Cord (3.7 m / 12.1 ft) Thermo Scientific 1300 Series A2...
- Page 55 93 / 3.66 Exhaust air Width mm / in 610 / 24.02 915 / 36.02 Depth mm / in 457 / 17.99 457 / 17.99 Height mm / in 117 / 4.61 117 / 4.61 1300 Series A2 Thermo Scientific...
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Page 56: Certification Testing
Velocity of the displacement flow circulating through the work chamber. Exhaust velocity (FPM): Velocity of the airflow discharged through the exhaust filter opening. Exhaust airflow volume (CFM): Amount of air discharged at the exhaust filter. Thermo Scientific Model 1300 Series A2... - Page 57 – electrical leakage, ground circuit resistance and polarity tests Note Service with costs: Unless certification was expressly called for in the specification, quotes and/or purchase order, the cost for this on-site testing is to be paid for by the customer. Model 1300 Series A2 Thermo Scientific...
- Page 58 3. Operate the system blowers for approx 20 min. 4. Record at least 5 measurements of the inflow air volume. 5. Average those readings and calculate the inflow velocity (V1) as described below. Thermo Scientific Model 1300 Series A2...
- Page 59 Inflow Velocity – Constricted Window Method (Secondary) Description: • Inflow velocity measured over several points through a constricted win- dow. Equipment: • Thermal Anemometer, or equivalent (hotwire) • Anemometer Probe Holder, part number 1911325 Model 1300 Series A2 Thermo Scientific...
- Page 60 • 3" access opening of 0.985 sq ft • 10" access opening 3.285 sq ft Refer to the data plate on the lower right corner of the unit face. Figure 8-1. Constricted Window Method Thermo Scientific Model 1300 Series A2...
- Page 61 5.87 5.87 5.87 5.87 5.87 5.87 5.87 inches Effective Exhaust Filter Area: • 4 ft models (1354/1355/1356/1384/1385/1386): 16.8” x 22.8”, 2.66 sq ft • 6 ft models (1357/1358/1359/1387/1388/1389): 16.8” x 34.8”, 4.06 sq ft Model 1300 Series A2 Thermo Scientific...
- Page 62 3. Record the readings on a horizontal plane 4 inches (10 cm) above the bottom edge of the window opening while in the work position, in a grid pattern not to exceed 6 inches (15 cm) x 6 inches (15 cm). Thermo Scientific Model 1300 Series A2...
- Page 63 • If the filter is in order, change the supply blower. Note Check of alarm limits: If S1 or S2 are changed, the alarm limits S3 and S4 have to be checked and saved again. Model 1300 Series A2 Thermo Scientific...
- Page 64 1. Set up as in Steps 1 through 5 above 2. In the duct, downstream of the filter, sample in accordance with NSF/ANSI Standard 49, 2004. Acceptance: • Filters not scanned – 0.005% of upstream concentration Thermo Scientific Model 1300 Series A2...
- Page 65 • No smoke shall have dead spots, reflux or escape the cabinet once drawn Elect. Leakage , Ground Description: Resistance, Polarity Tests • Refer to UL 61010-1 Site Installation Description: Assessment Tests • Verify the unit is integrated properly into the facility. 8-10 Model 1300 Series A2 Thermo Scientific...
- Page 66 Acceptance: • Unit alarm activates within 15 seconds when the downflow and inflow velocity is set to the correct values and the LED „Airflow STABLE“ changes from green to red (as described above). Thermo Scientific Model 1300 Series A2 8-11...
- Page 67 • Measure static pressure in the duct between the unit and balancing dampers. Acceptance: • Negative pressure exists Note Standard: For additional and detailed information regarding these tests, refer to NSF/ANSI Standard 49, 2004. 8-12 Model 1300 Series A2 Thermo Scientific...
- Page 68 Device type: Part number: Serial number: Service number: Location: Operator's note: Technician / Company Notes Date Signature Thermo Scientific 1300 Series A2...
- Page 72 Thermo Scientific Controlled Environment Equipment 401 Millcreek Road Marietta, Ohio 45750 United States www.thermo.com...