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Thermo Scientific GENESYS 10S Vis User Manual

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GENESYS 10S Vis
User Guide
269-251700 Revision A
October 2009

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Summary of Contents for Thermo Scientific GENESYS 10S Vis

  • Page 1 GENESYS 10S Vis User Guide 269-251700 Revision A October 2009...
  • Page 2 © 2008-2009 Thermo Fisher Scientific Inc. All rights reserved. Microsoft, Windows, Vista, Windows NT and Excel are either trademarks or registered trademarks of Microsoft Corporation in the United States and/or other countries. Clorox is either a trademark or registered trademark of The Clorox Company in the United States and/or other countries. Triton is either a trademark or registered trademark of Union Carbide in the United States and/or other countries.

  • Page 3: Table Of Contents

    Loading Paper in the Internal Printer ......22 Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 4 Measuring Samples ..........52 GENESYS 10S Vis User Guide...
  • Page 5 Taking Measurements ..........80 Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 6 Parameters ............117 GENESYS 10S Vis User Guide...

  • Page 7: Preface

    Preface Congratulations on your purchase of a Thermo Scientific spectrophotometer! Our spectrophotometers integrate advanced hardware features with the power and flexibility of a wide range of accessories. Safety and Special Notices Make sure you follow the precautionary statements presented in this guide. The safety and other special notices appear in boxes.
  • Page 8 Preface viii GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 9: Chapter 1 Spectrophotometer Basics

    Spectrophotometer Components Here are some major components visible on the outside of a typical instrument: Sample compartment USB port Optional printer housing Keypad Connectors The connectors are on the back of the instrument: Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 10 A/C power connector On/Off switch WARNING Avoid shock hazard. Always turn off the instrument and unplug it from the wall outlet or power strip before you unplug the power cord from the instrument connector. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 11: About The Keypad

    • Functions will change depending on the software screen. • Some function keys may not be active. • Clears the value being entered. • Returns to the previous screen. • Deletes the last character entered. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 12: Cell Holders

    • Selects the cell holder position to be measured. • B = blank and 1-5 = sample positions. • = positions when used in Auto 3 mode. Cell Holders Your instrument includes a 6-position and single cell holder. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 13: 6-Position Cell Holder

    190 nm to > 1100 nm Polystyrene > 340 nm Methacrylate > 300 nm Acrylic > 280 nm UV-transparent > 220 nm Note See the manufacturer’s specifications and work within the recommended range. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 14: Z-Dimensions

    The figure below illustrates the position of the light beam in the instrument. Beam size specifications are shown below. • Distance from bottom of cuvette to center of beam (Z-dimension): 8.5 mm • Beam dimensions: 2 mm (wide) by 7 mm (tall) GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 15: Setting Up The Instrument

    On/Off Toggle • Alphanumeric Entry Numeric Entry With the parameter (e.g., Wavelength) highlighted, start typing the numeric value. An Entry window with the value range appears. Type the complete entry and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 16: Menu Selection

    With the parameter (e.g., AutoPrint) highlighted, press Enter to toggle to the opposite value. Alphanumeric Entry With the parameter (e.g., Test Name) highlighted, press Enter. The Name Entry screen appears. Highlight the desired character and press Add Character. When you are finished, press Accept Name. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 17: Setting Utility Parameters

    • Press Utility on the keypad. Setting the Date and Time Highlight Date/Time Setup and press Enter. You can modify the date, time format and time. To set the date 1. Highlight Set Date and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 18 Note Your changes are saved automatically (even during power down) by battery backup. Since the tungsten lamp of the GENESYS 10S Vis spectrophotometer requires a 30-minute warm-up, the spectrophotometer has been pre-set at the factory to Standby Mode OFF. To change Standby Mode time 1.

  • Page 19: Setting Baseline Expiration Time

    Setting the Screen Contrast To make it easier to read the display, you can adjust the screen contrast on the instrument. To set the screen contrast 1. Highlight Screen Contrast and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 20: Setting Up The Internal Printer

    To ensure that the instrument can output information correctly to the printer, select the appropriate device. To set the utility parameters for the printer 1. Press Utility. 2. Highlight Printer and press Enter. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 21 Note Text and graphics can be output via the internal printer and an external printer on the USB port. Only text (no graphics) can be output via the USB connection to a computer. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 22 Setting Up the Instrument Setting Up the Internal Printer GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 23: Accessories

    Cell Holder Configurations The following table shows the available cell holders and their accessories. You can install or remove accessories without switching off the instrument. Cell Changer System Single Cell System Standard Cell Holders Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 24 50 mm Rectangular Long Pathlength Cell Holder Must be installed in the B, 2 and 4 positions 50 mm Cylindrical Long Pathlength Cell Holder Must be installed in the B, 2 and 4 positions GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 25 Cannot use 100 mm path cells with Cell Positioner Thin Film/Filter Holders Must be installed in the B, 2 and 4 positions Adjustable Filter/Lens Holder Must be installed in the B, 2 and 4 positions Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 26: Cell Holder Initialization

    To prevent the Fatal Error whenever removing the cell changer, always return to either the main menu or the test parameter menu of the test before removing the cell changer. Changing Cell Holders • use long pathlength cells (cylindrical or rectangular) GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 27: Installing The 6-Position Cell Holder And The Single Cell Holder

    1. Open the sample compartment door and let it rest on its hinge. 2. With one hand, carefully lower the cell holder straight down into the sample compartment. 6-Position Cell Holder Figure 1. Captive thumbscrew Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 28: Removing The 6-Position Cell Holder And The Single Cell Holder

    3. With your other hand, pull straight up on the cell holder and lift it out of the sample compartment 4. Close the sample compartment door. Installing Accessory Cell Holders Make sure that you have the correct cell holder baseplate installed. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 29: Installing The Internal Printer (Optional)

    CAUTION Avoid shock hazard. Turn off the instrument and disconnect the power cord from the outlet before installing the internal printer. Captive screw on lamp door Removing door from hinge Connecting wires to printer Hinge Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 30: Loading Paper In The Internal Printer

    11. Tighten the captive screw on the printer door to hold it securely in place. Loading Paper in the Internal Printer Paper roll holder Paper entry slot Finger tab Icon for paper direction GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 31: External Printers

    Note The instrument is compatible with most HP PCL printers; brands other than HP will also be supported. If the printer is not purchased from Thermo Scientific, it is your responsibility to determine if the printer is compatible with the instrument. Contact technical support or your sales representative for more information.
  • Page 32 Accessories External Printers GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 33: Sample Positioner Setting

    Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 34: Manual 6

    Note When you have the Cell Changer installed, the instrument always considers the material in the B position as a blank. This means that even after measuring your blank the first time, you can place samples only in positions 1 through 5. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 35: Chapter 5 Cell Correction

    Cell “B” of the cell changer. Cell Correction To run Cell Correction 1. Load the test type or stored test. 2. If Cell Correction is not visible, highlight More parameters and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 36 Discrete nms – Cell Correction is run on a blank and up to five sample cells for up to 31 user-specified, discrete wavelengths. 6. If you selected Scan mode in the preceding step, specify the Start Wavelength and the Stop Wavelength values. 7. Press Run Corr. to start Cell Correction. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 37: Specifying Wavelengths For Discrete Nms Mode

    2. Highlight Number of Matched Cuvettes and press Enter. 3. Specify the number of cells you are matching and press Enter. 4. Press Set nms to select the wavelengths for which Cell Correction will be run. A list of wavelengths appears. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 38 The application measures the other cells against the blank and records, stores and dates the measurements. From these measurements the application establishes the required correction factors, which are applied during all subsequent tests (if Cell Correction is activated). GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 39: Managing Stored Tests

    Naming a Test When saving a test, specify its file name using up to eight alphanumeric characters. To name a test 1. After setting the test parameters, highlight Test Name and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 40: Saving A Test

    After a method has been configured, there are two options for saving a test. The test method can be saved to an external memory device using the front USB port or to the internal memory of the spectrophotometer. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 41 To save a test in the instrument library 1. Press Save Test. A prompt appears: 2. Select the appropriate location and press Enter. 3. Create or enter a name for the test. Use the procedure in “Naming a Test” page Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 42: Loading Test Files

    You can load saved test files from the internal memory from the Utility screen. To load test files 1. To access all test files, press Utility. 2. Highlight Stored Tests Directory and press Enter. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 43: Lock/Unlock

    Enter the password and press Enter. Note To lock or unlock access to the file, you must enter the software password of this manual. Deleting a Test To delete a test, highlight it and press Delete Test. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 44 Managing Stored Tests Deleting a Test GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 45: Smartstart

    3. Highlight the appropriate test and press Select Test. An arrow sign “>” indicates the test has been selected for the SmartStart menu. Press Esc to return to the Utility screen, or power down the instrument. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 46 An arrow sign “>” indicates the tests selected for the SmartStart menu. Press Esc to return to the Utility screen, or power down the instrument. Note To remove tests from the SmartStart menu, see the procedure above for unselecting a test. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 47: Chapter 8 Concentration Units

    • IU • mg/L • pM/μL • mg/mL • ng/μL Custom units can also be created; for more information, see “Creating Custom Units” page To select the units 1. Highlight Units and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 48: Creating Custom Units

    • Add a character to the name of a unit • Accept the name of a unit 2. Follow steps 2 through 4 in “Naming a Test” page The new custom unit appears in the list of basic units. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 49: Chapter 9 Calculator Function

    3. Press the desired function (+, -, x or ÷). 4. Enter the second desired value and press Enter. You can only add, subtract, multiply, or divide two lines of numbers at a Note time Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 50 Calculator Function GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 51: Abs And %T Measurements-Basic A-%T-C

    When Basic ATC is set to Absorbance or % Transmittance, these capabilities are provided: • Setting the Wavelength • Measuring a Blank • Measuring Samples Setting the Wavelength To set the wavelength 1. Press Set nm or any number key to set the wavelength. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 52: Measuring A Blank

    (ABS) or percent transmittance (%T) measurement appears on the display. If a Single Cell Holder is installed, remove the blank and place the sample in the cell holder. The absorbance or %Transmittance measurement appears on the display. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 53: Abs And %T Measurements-Advanced A-%T-C

    Recalling a Test To recall a test 1. In the Advanced A %T C screen, press Stored Tests. 2. Highlight the test you want to recall and press Enter. Use this screen for: Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 54: Setting Up Test Parameters

    1 to 5. 3. Press Measure Blank. If a 6-Position Cell Holder is installed, it moves to the B position to measure the blank and returns to its previous position. 4. Press Measure Sample. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 55 Abs and %T Measurements—Advanced A-%T-C Taking Measurements The sample measurement appears. If a 6-Position Cell Holder is installed, press the cell position buttons to reposition the cell holder and measure the rest of the samples manually. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 56 Abs and %T Measurements—Advanced A-%T-C Taking Measurements GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 57: Basic Concentration Measurements-Basic A-%T-C

    When Basic ATC is set to Conc/Std or Conc/Factor, you can perform these tasks. • Setting the Wavelength and Mode • Measuring a Blank • Measuring a standard or entering a factor • Measuring Samples Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 58: Setting The Wavelength And Mode

    1. If necessary, press Change Mode to switch to the Concentration with Standard mode. If a 6-Position Cell Changer is installed, place the blank in the B position, and the standard in position 1. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 59: Using Conc/Factor To Measure Concentration

    1. If necessary, press Change Mode to switch to the Conc With Factor mode. 2. Press Units/Factor to set the factor and select the units. 3. Press Enter Factor. 4. Type the desired factor value. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 60: Measuring Samples

    The measurement appears. If the Single Cell Changer is installed, remove the blank and place the sample in the cell changer. The measurement appears. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 61: Concentration Measurements-Advanced A-%T-C

    To get started, press Test, highlight Advanced A-%T-C and press Enter. Recalling a Test To recall a test 1. Press Stored Tests. 2. Highlight the test and press Enter or Load Test to display the parameters for the selected test. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 62: Setting Up Test Parameters

    To measure a standard manually (using Manual 6 or Single Cell Holder) 1. Press Run Standard. 2. Enter the concentration of the standard and press Enter. 3. Insert the blank and standard. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 63: Entering A Factor

    4. Press Measure Sample. If a 6-Position Cell Holder is installed, press the cell position buttons to reposition the cell holder and measure the rest of the samples manually. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 64 Concentration Measurements—Advanced A-%T-C Measuring Samples GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 65: Scanning

    Expiration. Press Enter and set the desired time. To get started, press Test, highlight Scanning and press Enter. Recalling a Test To recall a test 1. Press Stored Tests. 2. Highlight the test you want to recall and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 66: Setting Up Test Parameters

    Note If a 6-Position Cell Holder is installed, be sure to place the blank in the B position. The instrument always uses the B position to collect the baseline. To collect a baseline scan 1. Press Run Test. 2. Place the blank in the B position. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 67: Scanning A Sample

    Note To switch between tabular and graphical displays, press Graph/Tabular. Note If Auto Save Data is ON, you must enter a Data File Name before you can access Run Test or Measure Samples. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 68: Viewing And Manipulating Scan Data

    • Use Auto Scale to change the scale and display the new graph. • Use Manual Scale to change the scale and display the new graph. • Use the cursor to identify specific points along the X-axis. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 69 1. Press Manual Scale to display the manual scale options. 2. To set the minimum or maximum value for the X- or Y-axis, press Min Y, Max Y, Min X or Max X. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 70: Performing Calculations On The Scan Data

    Calculating the Area Under a Curve • Determining Peak Height Using a 3-point Net Equation Labeling Peaks and Valleys To label valleys and peaks 1. With your scan displayed on the edit graph screen, press Math. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 71: Smoothing Data

    1. With your scan data displayed on the edit graph screen, press Math. 2. Press 3-Pt Net. The 3-point net measurement screen shows the cursor options and three cursor lines (designated for the left, center and right wavelengths). Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 72: Calculating The Area Under A Curve

    Calculating the Area Under a Curve To calculate the area under a curve 1. With your scan data displayed on the edit graph screen, press Math. 2. Press Area to display the Area Under the Curve measurement screen. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 73: Viewing And Rescaling Tabular Scan Data

    The instrument calculates the area under a curve for the selected wavelengths, factor and calculation method. Viewing and Rescaling Tabular Scan Data When working with tabular scan data, you must press Edit Data before performing other functions on the data. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 74 1. With your table of scan data displayed on the edit screen, highlight the appropriate data point in the table. 2. Press Start nm or End nm. The instrument highlights the selected data points. To display the plot using the highlighted data points, press Graph. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 75: Chapter 15 Multiwavelength

    To get started, press Test, highlight Multiwavelength and press Enter. Recalling a Test To recall a test 1. Press Stored Tests. 2. Highlight the test to recall and press Enter to display its parameters. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 76: Setting Up Test Parameters

    Note If Auto Save Data is ON, you must enter a Data File Name before you can access Run Test or Measure Samples. Adding Wavelengths and Factors Note You can enter factors only when the measurement mode is set to Concentration/Factor. To add wavelengths and factors 1. Press Set nms. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 77: Deleting Wavelengths And Factors

    You can access Multiwavelength acquisition from either the Set nms screen shown above or from the Multiwavelength setup screen. To take measurements automatically (Auto 6 or Auto 3) 1. Press Run Test to display the Multiwavelength measurement screen. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 78 If you set the measurement mode to Concentration/Factor, the calculated concentration at each wavelength also appears. If a 6-Position Cell Holder is installed, press Cell Position to reposition the cell holder and measure the rest of the samples manually. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 79: Chapter 16 Absorbance Ratio

    Measuring a Blank • Measuring Samples To get started, press Test, highlight Absorbance Ratio and press Enter. Recalling a Test To recall a test 1. In the Absorbance Ratio screen, press Stored Tests. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 80: Setting Up Test Parameters

    Note If Auto Save Data is ON, you must enter a Data File Name before you can access Run Test or Measure Samples. Measuring Samples To measure samples automatically (using Auto 6 or Auto 3) 1. Install the blank and samples. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 81 4. Press Measure Sample. If a 6-Position Cell Holder is installed, press the cell position buttons to reposition the cell holder and measure the rest of the samples manually. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 82 Absorbance Ratio Measuring Samples GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 83: Chapter 17 Absorbance Difference

    To get started, press Test, highlight Absorbance Difference and press Enter. Recalling a Test To recall a test 1. In the Absorbance Ratio screen (see “Using the Absorbance Ratio Screen” page 76), press Stored Tests. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 84: Using The Absorbance Ratio Screen

    Note If Cell Correction is ON, you must run the Setup Correction application before you can access Run Test or Measure Samples. Note If Auto Save Data is ON, you must enter a Data File Name before you can access Run Test or Measure Samples. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 85: Measuring Samples

    A 6-Position Cell Holder can hold five samples. 3. Press Measure Blank. If a 6-Position Cell Holder is installed, it moves to the B position to measure the blank and returns to its previous position. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 86 Absorbance Difference Measuring Samples 4. Press Measure Sample. If a 6-Position Cell Holder is installed, press the position buttons to reposition the cell holder and measure the rest of the samples manually. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 87: 3-Point Net

    Note If Cell Correction is ON, you must run the Setup Correction application before you can access Run Test or Measure Samples. Note If Auto Save Data is ON, you must enter a Data File Name before you can access Run Test or Measure Samples. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 88: Recalling A Test

    Taking Measurements To take measurements automatically (Auto 6 or Auto 3) 1. With the 3-Point Net Setup screen displayed and the parameters set, press Run Test to display the 3-Point Net measurement screen. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 89 4. Press Measure Sample. If a 6-Position Cell Holder is installed, press the cell position buttons to reposition the cell holder and measure the rest of the samples manually. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 90 3-Point Net Taking Measurements GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 91: Concentration Measurements-Standard Curve Application

    Curve: change the number of standards, select a different curve fit or delete points from the curve. • Viewing and saving sample data measured using the standard curve To get started, press Test, highlight Standard Curve and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 92: Recalling A Standard Curve

    Run Cell Correction. Measuring the Standards for a Standard Curve To measure standards automatically (using Auto 6 or Auto 3) 1. Install the blank and standards. 2. When the parameters are correct, press Run Standards. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 93 3. Set Entry concentration. 4. Press Measure Blank. If a 6-Position Cell Holder is installed, it moves to the B position to measure the blank and returns to its previous position. 5. Press Measure Standards. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 94: Using The Standards Screen

    1. Press Run Test. 2. Install the blank and samples. 3. Press Enter. The Standard Curve screen shows the absorbance and concentration of each sample. To switch between tabular and graphical displays, press View Graph/Tabular. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 95: Editing A Standard Curve

    Editing a Standard Curve You may edit the concentration of any standard on a standard curve. In addition, you may change the number of standards, select a different curve fit or delete points from the curve. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 96 1. With the standard curve displayed, highlight the standard to delete and press Edit Standards. 2. Highlight Delete Standard and press Enter. To clear measurements 1. With the standard curve displayed, press Edit Standards. 2. Highlight Clear Measurements and press Enter. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 97 1. With the standard curve you want to edit displayed as a graph, press Change Fit. 2. Highlight the curve fit to use for the standard curve and press Enter. The instrument applies the selected curve fit to the data and displays the new fit. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 98 Concentration Measurements—Standard Curve Application Editing a Standard Curve GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 99: Kinetics

    Note The Kinetics application lets you collect up to 400 data points per run. When setting test parameters, select the interval time and total run time accordingly. To get started, press the Test button, highlight Kinetics and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 100: Recalling A Test

    Saving a Test • Viewing the list of stored tests • Measuring a Blank • Measuring Samples Setting Up Test Parameters To set up test parameters 1. In the Kinetics screen, highlight the desired parameter. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 101: Measuring Samples

    To measure samples 1. In the Kinetics screen, press Run Test. 2. If a 6-Position Cell Holder is installed, place the blank in position B and the sample in position #1. 3. Press Measure Sample. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 102: Recalling And Recalculating Graphical Kinetics Results

    When your results are displayed, you can modify the range (start and stop time) and the instrument recalculates the reaction rate. When working with graphical kinetics results, you need to press Edit Graph before you can rescale and recalculate. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 103 The instrument also recalculates the results, using all the data, and displays them. To use Manual Scale 1. With your kinetics data displayed on the edit screen, press Manual Scale to display the manual scale options. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 104 2. Press Cursor ← or Cursor → to position the cursor line on the appropriate point on the graph. The data for the selected point appears. 3. When the cursor line is in the correct position, press Set Min X or Set Max X to accept the selected point. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 105: Rescaling And Recalculating Tabular Kinetics Results

    To select specific start and end times for the rate calculation 1. With your kinetics data displayed on the edit screen, highlight the appropriate data point in the table. 2. Press Start Time or End Time to display the recalculated rate and result. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 106 Kinetics Rescaling and Recalculating Tabular Kinetics Results GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 107: Chapter 21 Performance Verification

    You can also press Print to print another copy of the results. Accessing the Performance Verification Tests To access the Performance Verification tests 1. Press Tests. 2. Highlight Performance Verification and press Enter. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 108: Troubleshooting Checklist

    • The lamp compartment is clear of obstructions. WARNING Do not open the lamp compartment unless the instrument power is OFF. WARNING Do not turn the instrument power ON unless the lamp compartment is closed. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 109: Wavelength Accuracy - Internal

    %T mode. Typical wavelengths and tolerances are included in the firmware, but these values can be changed to match the calibration certificate included with your standards. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 110 1. Highlight the appropriate wavelength. 2. Press Delete nm. To run the test 1. Verify that the wavelengths and tolerances are set correctly. 2. Press Start Test. The results indicate pass or fail for each wavelength. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 111: Resolution

    The results indicate pass or fail for each wavelength. If the test fails, follow these guidelines: • Repeat the test twice to verify that the test is failing consistently. • Contact technical support for more troubleshooting advice. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 112: Photometric Accuracy

    To switch between absorbance and %Transmittance, press Change to %T (or Change to ABS) until the appropriate mode appears. Adding Standards You will need to set three values whenever you add a standard: the wavelength, the absorbance (or %Transmittance) and the tolerance value. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 113: Deleting Standards

    In the Photometric Accuracy screen, make sure the wavelengths, absorbance (or %T) values and tolerances are set correctly. To run the Photometric Accuracy test Press Start Test. The results indicate pass or fail for each wavelength. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 114: Noise

    4. Press Start Test. The results of the test indicate pass or fail for each wavelength. If the test fails, follow these guidelines: • Repeat the test twice to verify that the test is failing consistently. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 115: Stray Light

    • Verify that the filters are placed in the correct cell positions. • For more troubleshooting advice contact our sales or service representative in your area or use the information at the beginning of this document to contact us. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 116: Internal Printer Test

    (the outside surface of the roll is the thermal surface). • For more troubleshooting advice contact our sales or service representative in your area or use the information at the beginning of this document to contact us. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 117: Chapter 22 Maintenance

    • Always clean up spills as soon as they occur to prevent or minimize damage to the instrument. If concentrated acids or bases, or any hydrocarbon materials, are spilled on the instrument, clean up the affected area immediately. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 118: Cleaning And Maintaining Cells

    • Rinse with organic solvent • Warm water with detergent • Dilute nitric acid (<10%) rinse • Copious water rinse Organic Alcohol solutions • Rinse with similar alcohol, acetone, or other solvent • Copious water rinse GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 119 If a cell was cleaned in an ultrasonic bath, the warranty is void by the manufacturer. Thermo Scientific GENESYS 10S Vis User Guide...

  • Page 120: Cleaning The Windows Of The Sample Compartment

    2. Position the instrument so you can access the power entry module on the back of the instrument. 3. Remove the power cord. 4. Insert a flat-blade screwdriver into the notch on the fuse cover and pry off the cover. GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 121: Replacing The Tungsten Lamp

    Note If the fuse blows again, contact your distributor or technical support. Replacing the Tungsten Lamp WARNING This lamp gets very hot during operation. Before removing the lamp, turn off the instrument and allow the lamp to cool for 10 minutes. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 122 7. Release the tab and disconnect the cable. 8. Unpack the new lamp assembly. 9. Use a soft, lint-free cloth to wipe off any fingerprints on the lamp. 10. Connect the cable to the connector. GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 123 13. Close the lamp door. 14. Turn on the instrument and press the UTILITY key. 15. Press the arrow keys to highlight Lamp Hours and press ENTER. 16. Press ENTER to reset the lamp hours to zero. Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 124 Maintenance Replacing the Tungsten Lamp GENESYS 10S Vis User Guide Thermo Scientific...

  • Page 125: Parameters

    Turns the automatic printout on or off Autoscale Rescales the graph to the original ranges of the X- and Y-axes (Kinetics, Scanning) Base Sequence Sequence of bases contained in the sample (Oligos – calc factor tests) Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 126 Allows entry of a name for the data file when AutoSave = ON Date Cell Correction Displays the date when cell correction data on cuvettes was last collected Date Standards Measured Displays the date when standards were last measured with this instrument (Standard Curve tests) GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 127 Lets you select a portion of the data in a table for recalculation of a result (Kinetics and Scanning) Edit Graph Lets you manipulate the graph (Scanning) Edit Scale Lets you change the graph axis scales and view individual data points (Scanning) Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 128 (Utility) Intercept Enters where the line crosses the Y-axis (Abs where conc=0) Interval Enters the wavelength range between data points (Scanning tests only) Interval Time Enters the time between repeated readings (Kinetics only) GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 129 Accesses manipulation functions of the graph (Scanning) Measure Blank Initiates measurement of the blank (as function key) Measure Blank Selects the frequency of zeroing the instrument (as test parameter) Once or Every Reading (Kinetics) Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 130 Enters a reference wavelength value; for each reported measurement, measures the analytical wavelength and reference wavelength. Reported measurement = Abs@Analytical WL - Abs@Reference WL Ref. Wavelength Turns reference wavelength correction on or off Correction GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 131 Selects the factor entry or the baseline to calculate area under the peak in the graph (Scanning) Setup correction Initiates the procedure to collect the data necessary to correct for absorbance differences between cuvettes (all test types) Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 132 Tm value Calculates the melting temperature (Oligos tests) Total Run Time Enters the time from the Run initiation to the end of the test; equals Delay Time + Interval Times + Measurement Times (Kinetics) GENESYS 10S Vis User Guide Thermo Scientific...
  • Page 133 (all stored tests except Abs Ratio, Scanning, Cell Growth) Unselect Test Removes the “>” tag of the highlighted test name to remove the test from the SmartStart menu (Utility Stored Tests Directory) Wavelength Enters values for the analytical wavelengths Thermo Scientific GENESYS 10S Vis User Guide...
  • Page 134 Parameters GENESYS 10S Vis User Guide Thermo Scientific...

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