Page 1  Fax: +49 7462 2005 100  Internet: http://www.binder-world.com  E-mail: info@binder-world.com  Service Hotline: +49 7462 2005 555  Service Fax: +49 7462 2005 93 555  Service E-Mail: customerservice@binder-world.com  Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3 ...
Page 2: Table Of Contents
Water supply ........................41 4.5.1.2 Dehumidification ......................42 4.5.1.3 Types of suitable water quality ..................42 4.5.1.4 BINDER Pure Aqua Service (option) ................42 4.5.2 Water supply via freshwater bag (standard) ................43 4.5.2.1 Scope of delivery ......................43 4.5.2.2 Installation and connection of the freshwater bag ............
Page 3 6.6.1 Hypoxic control range 0.2 vol.-% up to 20 vol.- % O (regular) ..........70 6.6.2 Alternative control range 10 vol.-% up to 95 vol.-% (available via BINDER INDIVIDUAL Customized Solutions) ......................70 6.6.3 Operation without O control ....................71 Activating / deactivating CO and humidity control ..............
Page 4 Stopping a running time program ...................... 81 9.2.1 Pausing a running time program ..................... 81 9.2.2 Cancelling a running time program ..................81 Performance after the end of the program ..................81 Creating a new time program ......................82 Program editor: program management ..................... 82 9.5.1 Deleting a time program......................
Page 5 18.2 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ..139 18.3 Quick sample access (option) ......................140 18.4 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions) ..... 141 18.5 Analog outputs for temperature and CO (option) ................
Page 6 Humidity (CBF / CBF-UL) ..................... 172 22.6.8 Controller ..........................172 22.6.9 Open door ..........................173 22.7 Sending the chamber back to BINDER GmbH ................173 23. DISPOSAL......................174 23.1 Disposal of the transport packing ....................174 23.1.1 Outer chamber packing......................174 23.1.2...
Page 7 23.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of Germany............................176 23.5 Disposal of the chamber in non-member states of the EU ............. 178 24. TECHNICAL DESCRIPTION ................178 24.1 Factory calibration and adjustment ....................178 24.2 Over current protection ........................
Page 8: Safety
Operating Manual. This Operating Manual is supplemented and updated as needed. Always use the most recent version of the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-to- dateness and validity of this Operating Manual.
Page 9: Intellectual Property
In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual. This operating manual cannot cover all conceivable applications. If you would like additional information, or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or contact us directly, e.g.
Page 10: Safety Alert Symbol
1.4.2 Safety alert symbol Use of the safety alert symbol indicates a risk of injury. Observe all measures that are marked with the safety alert symbol in order to avoid death or injury. 1.4.3 Pictograms Warning signs Hot surface Explosive atmosphere Stability hazard Electrical hazard Gas cylinders...
Page 11: Word Message Panel Structure
1.4.4 Word message panel structure Type / cause of hazard. Possible consequences.  Instruction how to avoid the hazard: prohibition  Instruction how to avoid the hazard: mandatory action Observe all other notes and information not necessarily emphasized in the same way, in order to avoid disruptions that could result in direct or indirect injury or property damage.
Page 12: Type Plate
Figure 2: Position of additional labels on the CBF / CBF-UL Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER service for these replacements. Type plate Position of type plate: left chamber side (seen from front), at the bottom in the middle Nominal temp.
Page 13: General Safety Instructions On Installing And Operating The Co Incubator
(for Germany: DGUV guidelines 213-850 on safe working in laboratories, issued by the employers’ liability insurance association). BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
Page 14 Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature inside the chamber must lie below the flash point or below the sublimation point of the charging material. Familiarize yourself with the physical and chemical properties of the charging material, as well as the contained moisture constituent and its behavior with the addition of heat energy and humidity (CBF / CBF- UL).
Page 15: Precautions When Working With Gases
The chamber is equipped with a permanent magnet located behind the inner panel of the outer chamber door. If persons with active implants (e.g. pacemakers, defibrillators) keep a sufficient safe distance (distance of field source to implant), an influence of these implants can be excluded with high probability. WARNING Magnets can affect the function of pacemakers and implanted defibrillators.
Page 16 Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible. General information for safe handling of oxygen: • Make sure training of personnel on hazards of oxygen enrichment and necessary safety measures.
Page 17: Precautions When Handling Gas Cylinders
Precautions when handling gas cylinders General information for safe handling of gas cylinders: • Store and use gas cylinders only in well-ventilated locations. • Open the gas cylinder valve slowly to avoid pressure surges. • Secure gas cylinders during storage and use against falling (chaining). •...
Page 18: Intended Use
Such ingredients include in particular acids and halides. Any corrosive damage caused by such ingredients is excluded from liability by BINDER GmbH. The chamber does not dispose of any measures of explosion protection.
Page 19: Foreseeable Misuse
The requirements described in the Operating Manual for installation site and ambient conditions (Chap. 3.4) must be met. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.
Page 20: Residual Risks
• Bypassing or changing protective systems, operation of the chamber without the designated protective systems • Non-observance of messages regarding cleaning and disinfection of the chamber. • Spilling water or cleaning agent on the chamber, water penetrating into the chamber during operation, cleaning or maintenance.
Page 21: Operating Instructions
• Absence of a plausibility check to rule out erroneous inscription of electrical components • Performance of repair work by untrained/insufficiently qualified personnel • Inappropriate repairs which do not meet the quality standard specified by BINDER • Use of replacement parts other than BINDER original replacement parts •...
Page 22: Measures To Prevent Accidents
1.14 Measures to prevent accidents The operator of the chamber must observe the local and national regulations and take precautions to prevent accidents. The manufacturer took the following measures to prevent dangers: • Indications on the type plate See operating manual chap. 1.6. •...
Page 23: Cbf / Cbf-Ul : Resistance Of The Humidity Sensor Against Harmful Substances
1.15 CBF / CBF-UL : Resistance of the humidity sensor against harmful substances The following list of harmful substances refers only to the humidity sensor and does not include any other materials incorporated in the chamber or prohibited substances in relation to explosion protection. Some gases - especially clean gases - do not have any influence on the humidity sensor.
Page 24: Chamber Description
A highly precise, drift-free CO infrared measuring system in combination with the permanent mixture of CO gas through a special proprietary gas mixing head developed by BINDER allows precise and constant CO concentrations for long periods. This creates optimum growth conditions for cultures.
Page 25: Chamber Overview
A resistance humidifying system humidifies the air. For this purpose, use deionized (demineralized) water. The option BINDER Pure Aqua Service allows using the chamber with any degree of water hardness. Controller: The efficient program controller is equipped with a multitude of operating functions, in addition to recorder and alarm functions.
Page 26: Inner Chamber
Pt 100 temperature probe sensor (chamber with O control) Humidity sensor (CBF / CBF-UL) Internal socket 230V (max. 3 A) (option, available via BINDER INDIVIDUAL Customized Solutions, chap. 18.4) Perforated shelves, made of stainless steel Glass door handle Measuring access port Inner glass doors Permadry™...
Page 27: Control Panel On The Rear Of The Chamber
Pump (CBF / CBF-UL) Water connection for silicone tube (CBF / CBF-UL) Opening for freshwater bag (CBF / CBF-UL) Control panel on the rear of the chamber (10) (11) (1b) (6b) Figure 6: Rear control panel CB with O control and options (1b) (10) (11)
Page 28: Instrument Panel
Strain relief for power cable Quick acting closure socket for N (chamber with O control) (10) Quick acting closure socket for CO (11) Quick acting closure socket for O (chamber with O control with optional alternative control range 10 up to 95 vol.-% O Instrument panel MB2 controller display 5,7"...
Page 29: Guidelines For Safe Lifting And Transportation
Note on second-hand chambers (Ex-Demo-Units): Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly. Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before commissioning the chamber.
Page 30: Location Of Installation And Ambient Conditions
The chambers can be stacked on top of each other (two chambers maximum). For safe stacking that is easy to maintain, use the original BINDER stacking stand (chap. 18.9.1) or the base on castors (chap. 18.9.2).
Page 31 NOTICE Danger of overheating due to lack of ventilation. Damage to the chamber. ∅ Do NOT install the chamber in unventilated recesses.  Ensure sufficient ventilation for dispersal of the heat.  Observe the prescribed minimum distances when installing the chamber. Do not install or operate the chamber in potentially explosive areas.
Page 32 DANGER Danger of suffocation and poisoning by high concentration of CO (> 4 Vol.-%). Death by suffocation. ∅ Do NOT set up chambers in non-ventilated recesses.  Ensure technical ventilation measures.  Observe the relevant regulations for handling CO Observe the occupational exposure limit OEL for CO set by the national authorities (formerly maximum permitted workplace concentration).
Page 33: Installation And Connections
General information for safe handling of oxygen: • Make sure training of personnel on hazards of oxygen enrichment and necessary safety measures. • Make sure adequate labeling of all oxygen equipment and facilities. • Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak spray or diluted soap solution).
Page 34: Permadry™ Water Pan
Permadry™ water pan The Permadry™ system developed by BINDER is an effective and easy to handle system that ensures high humidity inside the chamber without any condensation forming on the inner surfaces. The Permadry™ water pan consists of two pans in which the outer one is heated and the inner one cooled. With the slight difference of temperature caused by that cooling, the central pan is the specific point for condensation of the surplus humidity.
Page 35: Connecting The O
Connecting the O sensor (chamber with O control) The O sensor is supplied with the chamber in a separate package. Connect or remove the O sensor only when the chamber is turned off. Open the door of the inner chamber and plug the O sensor (F) into the left connection socket located in the upper part of the rear of the inner chamber.
Page 36: Connection Of The Co Gas Cylinder
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution). 4.4.1 Connection of the CO gas cylinder Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible.
Page 37: Connection Of The O Gas Cylinder (Chamber With O Control With Optional Alternative Control Range 10 Up To 95 Vol.-% O )
Leak test After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution). The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 24.4) and refer to a connection pressure of 2.0 bar / 29 psi.
Page 38: Connection Of The N Gas Cylinder (Chamber With O Control)
NOTICE Danger of damage by excessive outlet pressure > 2.5 bar / 36 psi. Damage to the chamber. ∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.  Before connecting, check the outlet pressure on the pressure reducer of the cylinder. ...
Page 39: Connecting The Gas Hose To The Chamber Rear (For Co )
The following steps are required: Ensuring the correct N output pressure A gas supply pressure above 2.5 bar / 36 psi will result in chamber damage. Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the chamber.
Page 40 Only use the supplied hose nozzle to connect to the quick acting closure socket. Otherwise, the quick acting closure socket may leak, and/or it may become impossible to connect the original hose nozzle. In this case, please contact BINDER Service. Remove the rubber cover (b) by pulling it off.
Page 41: Gas Cylinder Connection Kits (Option)
4.4.5 Gas cylinder connection kits (option) Gas cylinder connection kits are available for CO (Art. No. 8012-0014), O (Art. No. 8012-0015) and N (Art. No. 8012-0016). The connection kit includes the following parts for connecting a gas cylinder to the CO incubator: •...
Page 42: Dehumidification
1 µS/cm (ultrapure water), may cause acid corrosion due to its low pH). • Tap water that has been treated by the optional water treatment system BINDER Pure Aqua Service (disposable system). A reusable measuring equipment to assess the water quality is included (chap.
Page 43: Water Supply Via Freshwater Bag (Standard)
4.5.2 Water supply via freshwater bag (standard) 4.5.2.1 Scope of delivery The optional tubular bag set includes all necessary connecting parts: • Set art. no. 8009-1083 with 3 freshwater bags (included in scope of delivery) • Set art. no. 8009-1058 with 12 freshwater bags (option, annual demand) Each bag set includes: •...
Page 44: Refilling The Already Installed Freshwater Bag
Please proceed in the following order: • Fill the freshwater bag. Observe water quality and temperature (chap. 4.5.1.3). • Attach a tube connector part with a 5-7 mm nozzle to the freshwater bag ① • Attach a tube connector part with a 3-5 mm nozzle to the silicone tube ② •...
Page 45: Replacing The Tube Connectors For Freshwater Supply
Procedure with optional filling hose A bag set (Art.no. 8009-1058) incl. accessories (annual demand) is optionally available (chap. 4.5.2). For easier filling, the set contains a filling tube (DN 10 mm) of 1 m length with a fitting tube connector. •...
Page 46: Water Supply Via Prefilled Water Bottle (Optional Humidification Water Set)
4.5.3 Water supply via prefilled water bottle (optional humidification water set) By using the humidification water set you will achieve unsurpassed contamination safety of your samples in the BINDER CO incubator CBF / CBF-UL. 4.5.3.1 Scope of delivery The optional humidification water set includes all necessary connecting parts: •...
Page 47 Please proceed in the following order: • Turn the pump head (12) counterclockwise until it stops and remove it from the pump connector (13). • Pull up the tongue of the tube retainer (12b) up to “Click” and remove it. Take the rotor (12c) out of the pump head cover (12a).
Page 48: Water Supply Via Freshwater Can (Optional Water Container Set)
4.5.4 Water supply via freshwater can (optional water container set) The fresh water can, which is placed in an included magnetic can holder on the left chamber side, serves to supply one or several chambers with freshwater for humidification. Since the pump is self-priming, it is not required that the water outlet of the can should be placed higher than the pump, so you can position the water can freely in height.
Page 49: Scope Of Delivery
4.5.4.1 Scope of delivery The optional water container set includes all necessary connecting parts: • Art. no. 8009-1128 Set with freshwater can, magnetic support and connecting parts The water container set includes: • Magnetic can holder adjustable in height, consisting of magnetic holder (art.no. 8022-0009) and water can support (art.no.
Page 50 The end of the tube for connecting to the incubator should be about 6 cm long. • Press the tube retainer (12b) into the pump head cover until it snaps into place. to water can to incubator connection Figure 22: Inserting the silicone tube into the pump head ② •...
Page 51: Refilling The Freshwater Can
Please proceed in the following order: • Assemble the magnetic can holder (18): Attach the water can support to the holder • Attach the magnetic can holder (18) to the left chamber side • Insert the freshwater can (17) from above into the water can support •...
Page 52: Electrical Connection
• Use only the original connection cables from BINDER according to the above specification. • Prior to connection and start-up, check the power supply voltage. Compare the values to the specified data located on the chamber’s type plate (left chamber side, bottom right-hand, see chap.
Page 53: Divided Inner Door With Cell Therapy Compartmentation (Optional)
Divided inner door with cell therapy compartmentation (optional) With this option, the chambers are equipped with a divided inner door and additional dividers for compartmentation to prevent mixing up of sample vessels. Figure 24: A look inside an incubator with the ”Divided inner door with cell therapy compartmentation” option 4.7.1 Special model CBF 260 “Cell Therapy”...
Page 54: Handling And Aligning The Divided Inner Door
4.7.2 Handling and aligning the divided inner door Rubber profile gasket of individual glass door of the divided inner door Hinge of the set-in divided inner door, with elongated holes to align the inner door Measuring access port Figure 26: Divided inner door (example: CB 170) Pull the handles to open each of the individual glass doors.
Page 55: Mounting The Partition Walls For Cell Therapy Compartmentation
4.7.3 Mounting the partition walls for cell therapy compartmentation Figure 27: Divided inner door with shelves and partition walls (example, chamber sizes 56 and 170) The vertical partition wall is used to compartmentalize the interior of the chamber in connection with the divided inner door.
Page 56 Figure 30: Inserting the partition wall Then slide the partition towards the back of the chamber until it clicks into place. Rear Rear Figure 31: Partition wall, size 56, assembled Figure 32: Partition wall, size 170 / 260, assembled Figure 33: Shelf holder with inserted partition walls (example, size 170) CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 56/199...
Page 57: Functional Overview Of The Mb2 Chamber Controller
(CBF / CBF-UL), and specifying special controller functions for each program section. You can enter values or programs directly at the controller or use the APT-COM™ 4 Multi Management Software (option) specially developed by BINDER. Operating mode Temperature values...
Page 58: Operating Functions In Normal Display
Operating functions in normal display Current operating mode Text list for information icons Date, time, authorization level of the logged-in user, memory Quick setpoint entry Continue to next screen Back to Normal display Information Program start Setpoint entry Event list Display of active alarms Access to main menu Figure 35: Operating functions of the MB2 controller in normal display (sample values, CBF / CBF-UL with...
Page 59: Display Views: Normal Display, Program Display, Chart-Recorder Display
Display views: Normal display, program display, chart-recorder display Press the Change view icon to toggle between normal display, program display and chart- recorder display. Press the Normal display icon to return from program display and chart recorder display back to Normal display.
Page 60: Controller Icons Overview
Controller icons overview Navigation icons in Normal display Icon Signification Function Main menu Access from Normal display to the main menu Alarm Access from Normal display to the list of active alarms Access from Normal display to the event list Event list Access from Normal display to the setpoint entry menu: setpoint Setpoint setting...
Page 61 Functional icons in the chart recorder display Icon Signification Function Show legend Show legend Hide legend Hide legend Switch legend Switch between legend pages Show indication “Door open” (B2) Show indications Hide indications Hide indication “Door open” (B2) Pause chart recorder and change to history display. Data History display recording continues.
Page 62: Operating Modes
Operating modes The MB2 program controller operates in the following operating modes: • Idle mode The controller is not functional, i.e., there is no heating, no CO inlet or O inlet (O control) and no humidification (CBF / CBF-UL). The chamber approximates ambient values. You can activate and deactivate this operating mode with the “Idle mode”...
Page 63: Controller Menu Structure
Controller menu structure Use the navigation icons in the screen footer in Normal display to access the desired controller functions. The available functions depend on the current authorization level “Service”, “Admin” or “User” (chap. 13.1). This is selected either during login or can be available without password protection. Main menu: program settings, further information, “Service”...
Page 64: Main Menu
• Access to service data, controller reset to factory settings (chap. 5.5.3) • Available only for users with “Service” and “Admin” authorization level. Full functional range only for BINDER Service (users with “Service” authorization level). “Programs” submenu • Access to the controller’s program functions (chap. 8, 9, 10)
Page 65: Settings" Submenu
5.5.3 “Service” submenu The “Service” submenu is available for users with “Service” or “Admin” authorization level. When logged-in with “Admin” authorization level the user will find information to tell the BINDER Service in service case. Path: Main menu > Service Serial number of the chamber, setup version chap.
Page 66: Principle Of Controller Entries
Principle of controller entries In the selection and entry menus there are icons displayed in the footers which you can use to take over the entry or cancel it. Selection menu (example) Entry menu (example) After completing the settings there are the following possibilities: Press the Confirm icon to take over the entries and exit the menu or continue the menu sequence.
Page 67: Performance When Opening The Door
To reduce odors quickly we recommend heating up the chamber to its nominal temperature for one day and in a well-ventilated location. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.
Page 68: Controller Settings Upon Start Up
Controller settings upon start up The window „Language selection“ enables the language selection, in case that it’s activated in the “Start- up” menu. Afterwards occurs a request of the time zone and the temperature unit. The controller will function in the operating mode, which was active before the last shut-down. It controls temperature, CO (chamber with O control) and humidity (CBF / CBF-UL) in fixed value operating...
Page 69: Factory Settings
Factory settings The chamber is supplied with the following basic preset parameters: Temperature setpoint 37 °C / 98.6 °F • setpoint 5 vol.-% • setpoint (chamber with O control) 21 vol.-% • Humidity setpoint (CBF / CBF-UL) 93 % r.h. •...
Page 70: Operating The Duodoor™ Door Lock
Even with activated O control no O pressure alarm will be emitted. 6.6.2 Alternative control range 10 vol.-% up to 95 vol.-% (available via BINDER INDIVIDUAL Customized Solutions) In addition to the CO connection, the chamber provides gas connections for O and N •...
Page 71: Activating / Deactivating Co And Humidity Control
You can deactivate the N pressure alarm in the controller (chap. 7.3). Then also with activated O control no N pressure alarm will be emitted. • Alternating operation in the hyperoxic and hypoxic range or in the range from 20 vol.-% O up to 22 vol.-% O and O...
Page 72: Cb / Cb-Ul: Humidity Control Of The Permadry™ System
If the ambient temperature deviates by more than +/- 5 °C from the values recommended by the manufacturer, the conditions for maximum air humidity with condensation-free inner chamber are no longer guaranteed. Contact BINDER Service for assistance. CB / CB-UL, CBF / CBF-UL (E7) 01/2022...
Page 73: Set-Point Entry In "Fixed Value" Operating Mode
Set-point entry in “Fixed value” operating mode In Fixed value operating mode you can enter set-points for temperature, CO and O (chamber with O control) and humidity (CBF / CBF-UL), and the switching-state of up to 16 operation lines. All settings made in Fixed value operating mode remain valid until the next manual change. They are saved also when turning off the chamber or in case of toggling to Idle Mode or Program Mode.
Page 74: Set-Point Entry Through The "Setpoints" Menu
Set-point entry through the “Setpoints” menu Press the Setpoint setting icon to access the “Setpoint” setting menu from Normal display. “Setpoints” menu. Select “Fixed value operation setpoints” to access the individual parameters. • Select the field “Temperature” and enter the desired temperature setpoint. Setting range: 20 °C up to 60 °C.
Page 75: Direct Setpoint Entry Via Normal Display
Direct setpoint entry via Normal display Alternatively you can also enter the setpoints directly via Normal display. Normal display. Select the setpoint you want Example: “Temperature” entry menu. Enter the to change. desired setpoint and confirm entry with Confirm icon. When entering a humidity setpoint value >...
Page 76: Special Controller Functions Via Operation Lines
• Operation line “N2 pressure alarm off” activates / deactivates the N pressure alarm (chamber with O control) • Operation line “Interior socket” activates / deactivates interior socket (option, available via BINDER INDIVIDUAL Customized Solutions) The other operation lines are without function.
Page 77: Safety Instructions For Setting High Gas Concentrations
Safety instructions for setting high gas concentrations Notes on handling carbon dioxide (CO Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation or a suitable connection to an exhaust system.
Page 78: Timer Program: Stopwatch Function
Timer program: stopwatch function During an entered duration the controller constantly equilibrates to the setpoints entered in Fixed value operation mode (temperature, CO (chamber with O control), humidity (CBF / CBF-UL), configuration of the operation lines). This duration can be entered as a “Timer program”. During the program runtime, any setpoint changes do not become effective;...
Page 79: Stopping A Running Timer Program
Stopping a running timer program 8.2.1 Pausing a running timer program Press the Program pause icon to interrupt the program. The program is paused. The program runtime stops running down, the time display flashes. There are the following options: Press the Program start icon to continue the program Press the Cancelling icon to cancel the program 8.2.2 Cancelling a running timer program...
Page 80: Time Programs
Time programs The MB2 program controller permits programming time programs with real-time reference. It offers 25 program memory positions with up to 100 program sections each. For each program section you can enter set-points for temperature, CO (chamber with O control), and humidity (CBF / CBF-UL), section duration, type of set-point transition (ramp or step) and the tolerance ranges.
Page 81: Performance During Program Delay Time
9.1.1 Performance during program delay time During the configured program delay time until program start, the controller equilibrates to the current setpoints of Fixed value operation mode. Modifications of these setpoints are effective. When the configured moment for program start is reached, the program delay time ends and the program starts running.
Page 82: Creating A New Time Program
Creating a new time program Path: Main menu > Programs > Time program “Time program” menu: Enter the program name and, if desired, overview of the existing programs. additional program information in the corresponding fields. Select an empty program place. Press the Confirm icon.
Page 83: Deleting A Time Program
Program editor: “Edit program” menu Select the desired function and press the Confirm icon. The program editor offers following options: • Change the program name • Copy program • Replace program: Replacing an new or an existing program with the copied program. This menu point is visible only after a program has been copied.
Page 84: Section Editor: Section Management
Section editor: section management Path: Main menu > Programs > Time program Select the desired program.    Program view. Section view (example: section 1). Select the desired program section There are the following options: (example: section 1)  Select a parameter to enter or modify the according value (chap.
Page 85: Add A New Program Section
9.6.1 Add a new program section Section editor: “Edit section” menu. Select “Create new section” and press the Confirm icon. Then select whether to insert the new section before or after the current section. Press the Confirm icon. The new section opens. 9.6.2 Copy and insert or replace a program section Program view.
Page 86: Deleting A Program Section
Press the Edit icon to open the section editor if you want the current section to be replaced or the copied section to be inserted before or after it Program view. Section view (example: section 1). Select the section to be replaced or before or Press the Edit icon to open the section editor after which the copied section shall be inserted (example: section 3) and press the...
Page 87: Value Entry For A Program Section
Value entry for a program section Path: Main menu > Programs > Time program Select the desired program and section. The section view gives access to all parameters of a program section. You can enter or modify the values. Program name and section number Section duration Type of setpoint transition: ramp or step Operation lines...
Page 88: Set-Point Ramp And Set-Point Step
9.7.2 Set-point ramp and set-point step You can define the type of transitions for temperature, CO concentration, O concentration (chamber with control) and humidity (CBF / CBF-UL) for each individual program section. “Ramp” mode: Gradual set-points changes The set-point of a given program section functions as the section’s start value. During the section’s duration, each set-point gradually passes to the set-point of the subsequent program section.
Page 89 Selecting the setting “Ramp” or “Step” Section view (partial view). In the field “Course” select the desired setting “Ramp” or “Step”. “Ramp” and “Step” mode example (representation of a temperature course) ------------1-------------------------2------------------------3------------4----------5------------ W/°C 40.0 39.5 39.0 38.5 38.0 37.5 37.0 t/min.
Page 90: Special Controller Functions Via Operation Lines
• Operation line “N2 pressure alarm off” activates / deactivates the N pressure alarm (chamber with O control) • Operation line “Interior socket” activates / deactivates interior socket (option, available via BINDER INDIVIDUAL Customized Solutions) The other operation lines are without function.
Page 91: Setpoint Entry
9.7.4 Setpoint entry • Select the field “Temperature” and enter the desired temperature setpoint. Setting range: 20 °C up to 60 °C. Confirm entry with Confirm icon. The controller returns to the section view. • Select the field “CO2” and enter the desired CO setpoint.
Page 92: Repeating One Or Several Sections Within A Time Program
• Select the field “Tolerance band min” and enter the desired lower tolerance band value. Setting range: -99999 to 99999. Confirm entry with Confirm icon. The controller returns to the section view. • Select the field “Tolerance band max” and enter the desired upper tolerance band value. Setting range: -99999 to 99999.
Page 93: Saving The Time Program
9.7.7 Saving the time program Section view. Program view. After the all desired values of the program Press the Confirm icon to take over the section have been configured, press the programming. Confirm icon to take over the programming. The controller changes to the Normal display. The controller changes to the program view.
Page 94: Cancelling A Running Week Program
After completing the settings, press the Confirm icon to take over the entries and exit the menu. The program starts running. If instead you press the Close icon to exit the menu without taking over the entries, the program will not start.
Page 95: Program Editor: Program Management
Program view. For the first section no weekday is specified. Therefore the section is first marked in red and cannot be saved. 10.4 Program editor: program management Path: Main menu > Programs > Week program    “Week program” menu: Program view (example: program 1).
Page 96: Deleting A Week Program
The program editor offers following options: • Change program name. This menu also offers to configure the ramp / step mode setting (chap. 10.6.1). • Copy program • Replace program: Replacing an new or an existing program with the copied program. This menu point is visible only after a section has been copied.
Page 97: Section Editor: Section Management
10.5 Section editor: section management Path: Main menu > Programs > Week program Select the desired program.    Program view. Section view (example: section 1). Select the desired program section There are the following options: (example: section 1) ...
Page 98: Add A New Program Section
10.5.1 Add a new program section Section editor: “Edit section” menu. Program view. Select “Create new section” and press the With a new section no weekday is specified. Confirm icon. Therefore the section is first marked in red and cannot be saved. A new section is always added at the very bottom (example: section 3).
Page 99: Deleting A Program Section
Select “Replace section” to replace the selected section with the copied section Select “Insert section” to additionally add the copied section. Press the Confirm icon. If you selected “Insert section” the sections are automatically arranged in the correct chronological order. Section editor: “Edit section”...
Page 100: Weekday
“Change program name” menu. In the field “Course” select the desired setting “Ramp” or “Step” and press the Confirm icon. 10.6.2 Weekday In the field “Weekday” select the desired weekday. With “Daily” selected, this section will run every day Section view. at the same time.
Page 101: Setpoint Entry
• Operation line “N2 pressure alarm off” activates / deactivates the N pressure alarm (chamber with O control) • Operation line “Interior socket” activates / deactivates interior socket (option, available via BINDER INDIVIDUAL Customized Solutions) The other operation lines are without function.
Page 102: Notification And Alarm Functions
Notification and alarm functions 11.1 Notification and alarm messages overview 11.1.1 Notifications Notifications are indicated by information icons displayed in the screen header in Normal display An information icon serves as an indication of a certain condition. If this condition persists, in some cases an alarm will be triggered after a fix or configurable interval. As long as the condition persists, the information icon therefore continues to be displayed also in state of alarm.
Page 103: Alarm Messages
11.1.2 Alarm messages Start after Zero-voltage relay Condition Alarm message condition occurred alarm output Having reached the setpoint, the actual temperature value deviates by more than the set tolerance range value and longer than the set delay After configurable time from the setpoint “Temperature delay time (chap.
Page 104 Contact BINDER Service. Chamber with O control: “O2 sensor fault” immediately immediately sensor defective. Contact BINDER Service CBF / CBF-UL: “Humid sensor fault” immediately immediately Humidity sensor defective. Contact BINDER Service Hot air sterilization running “Sterilization”...
Page 105: State Of Alarm
Alarm messages are displayed in the list of active alarms until acknowledging them. They are also shown in the event list. When operating the chamber without CO connection, turn off the corresponding gas control in the “setpoints” menu (chap. 6.7) in order to avoid tolerance range and pressure alarms.
Page 106: Activating / Deactivating The Audible Alarm (Alarm Buzzer)
When the alarm condition has ended, the visual alarm indication is automatically cleared. The alarm is then no longer in the list of active alarms. • Acknowledging after the alarm condition has ended: The buzzer and the visual alarm indication are reset together.
Page 107 Alarms following deviations from the tolerance range or door opening If the actual value, after having reached the setpoint, deviates by more than the set tolerance range value and longer than 10 min. from the setpoint or the temperature doesn’t reach the tolerance range within 3 hours from turning on the chamber or closing the door, after the configured interval (e.g., “Temp range alarm delay”) an alarm is triggered.
Page 108: Zero-Voltage Relay Alarm Output
11.6 Zero-voltage relay alarm output The chamber is equipped with a zero-voltage relay output which permits the transmission of some alarm messages to a central monitoring system. The connection is realized as a DIN socket (3) on the chamber rear. A suitable DIN plug is enclosed.
Page 109: Temperature Safety Devices
The user cannot restart the device again. The protective cut-off device is located internally. Only a service specialist can replace it. Therefore, please contact an authorized service provider or BINDER Service. 12.2 Overtemperature safety controller class 3.1 The chambers are regularly equipped with an electronic overtemperature safety controller (temperature safety device class 3.1 according to DIN 12880:2007).
Page 110: Safety Controller Modes
12.2.1 Safety controller modes You can select between “Limit (absolute)” and “Offset (relative)” safety controller mode • Limit: Absolute maximum permitted temperature value This setting offers high safety as a defined temperature limit will not be exceeded. It is important to adapt the safety controller set-point after each modification of the temperature set-point.
Page 111: Message And Measures In The State Of Alarm
Regularly check the safety controller setting for set-point type “Limit” or “Offset” • in Fixed value operating mode according to the entered set-point temperature value • in program mode according to the highest temperature value of the selected temperature program Set the safety controller set-point by approx.
Page 112: User Management
• All passwords can be changed in the “log out” submenu (chap. 13.3). “Service” authorization level • Authorization level only for BINDER service • Extensive authorization for controller operation and configuration, access to service data • The passwords for “Service”, “Admin” and “User” authorization levels can be changed in the “log out”...
Page 113 Operation after user login At user login, the authorization level is selected and confirmed by entering the respective password. Following user login, controller operation is available, recognizable by the open-lock icon in the header. The available controller functions correspond to the user’s authorization level. Password protection activated for all levels: operation without user login is locked If passwords have been assigned for all authorization levels, the controller is locked...
Page 114 Information window To check the authorization level of the user currently logged-in, select in Normal display the arrow far right in the display header. The information window shows date and time, the controller’s free memory space and under “Authorization” the authorization level of the current user. If passwords have been assigned for all authorization levels, a user without login (password entry) has no authorization.
Page 115: Log In
13.2 Log in Path: Main menu > User > Log in Controller without a user logged-in Selection of user type (example) All selection possibilities are password protected Controller with logged-in user After completing the settings, press the Confirm icon to take over the entries and exit the menu, or press the Close icon to exit the menu without taking over the entries.
Page 116: Log Out
13.3 Log out Path: Main menu > User > Log out User logoff with “Admin” authorization Controller Controller with without a user logged-in user logged-in User logoff with “Admin” authorization Controller Controller with without a user logged-in user logged-in 13.4 User change If the password function has been deactivated (chap.13.5.2) this function is not available.
Page 117: Password Assignment And Password Change
User selection (example) All selection possibilities are password protected Controller with logged-in user 13.5 Password assignment and password change This function is not available for a user logged-in with “User” authorization. 13.5.1 Password change A logged-in user can change the passwords of his current level and of the next lower level(s). Example: A user with “Admin”...
Page 118 Selection of the authorization level Enter desired password. If desired, press the Change (example: view with “Admin” authorization) keyboard icon to access other entry windows. In the “Keyboard switch” window you can select different keyboards to enter uppercase and lowercase letters, digits, and special characters.
Page 119: Deleting The Password For An Individual Authorization Level
13.5.2 Deleting the password for an individual authorization level A user logged-in with “Service” or “Admin” authorization can delete the passwords of his current level and of the next lower level(s). To do this no password is entered during a password change. Path: Main menu >...
Page 120: New Password Assignment For "Service" Or "Admin" Authorization Level When The Password Function Was Deactivated
13.5.3 New password assignment for “service” or “admin” authorization level when the password function was deactivated If the password protection for an authorization level has been deactivated, i.e., no password is assigned, no login for this level is possible. Therefore access to this authorization level is available without login. If the password for the “Service”...
Page 121: Activation Code
13.6 Activation code Certain functions of the controller can be unlocked with a previously generated activation code. The activation code enables access to functions available only in the “Service” authorization level by users without a “Service” authorization. Such functions include e.g., adjustment or extended configurations. The activation code is available in authorization levels.
Page 122: General Controller Settings
General controller settings Most of the general settings can be accessed in the “Settings” submenu, which is available for users with “Service” or “Admin” authorization level. It serves to enter date and time, select the language for the controller menus and the desired temperature unit and to configure the controller’s communication functions.
Page 123 Or later: Path: Main menu > Settings > Date and time “Date and time” submenu. “Date / time” entry menu. Select the field “Date / time”. Enter date and time and press the Confirm icon. “Date and time” submenu. “Date and time” submenu. In the field “Daylight saving time switch”...
Page 124: Selecting The Temperature Unit
14.3 Selecting the temperature unit Following start-up of the chamber: Or later: Path: Main menu > Settings > Chamber Select the desired temperature unit and press the Confirm icon. Change of the temperature unit between °C and °F. If the unit is changed, all values are converted accordingly C = degree Celsius 0 °C = 31°F...
Page 125: Touchscreen Calibration
• Select the field “Brightness”. Move the grey slide to the left or right to define the brightness of the display • left = darker (minimum value: 0) • right = brighter (maximum value: 100) Press the Confirm icon. • Select the field “Wait time for screen saver” and enter the desired waiting time for the screen saver in seconds.
Page 126: Network And Communication
This menu allows to configure the communication parameters of the RS485 interface. The device address is required to recognize chambers with this interface type in a network, e.g. when connecting it to the optional BINDER APT-COM™ 4 Multi Management Software (chap. 18.1). In this case do not change the other parameters.
Page 127: Ethernet
14.5.2 Ethernet 14.5.2.1 Configuration Path: Main menu > Settings > Ethernet “Ethernet” submenu. • In the field “IP address assignment” select the desired setting “Automatic (DHCP)“ “Manual“. With selection “Manual” you can enter the IP- address, the subnet mask and the standard gateway manually.
Page 128: Display Of Mac Address
This controller menu serves to configure the web server. Then you can enter the chamber’s IP-address in the Internet. The IP address is available via Ethernet. The BINDER web server Chamber information > opens. Enter the user name and password which have been assigned for the web server in the controller menu.
Page 129: E-Mail
14.5.4 E-Mail As soon as an alarm was triggered, an e-mail is sent to the configured e-mail address. Path: Main menu > Settings > Email E-mail address entry: “Email” submenu. Select the desired e-mail address field and enter the e-mail address. You can use the Keybord change icon for entry.
Page 130: Usb Menu: Data Transfer Via Usb Interface
14.6 USB menu: Data transfer via USB interface The USB port is located in the instrument box. When you insert a USB-stick, the “USB” menu opens. The USB stick must be formatted with FAT32 and have at least 8GB of memory. Depending on the user’s authorization level, different functions (highlighted in black) are available for the logged-in user.
Page 131: General Information
General information 15.1 Service contact page Path: Main menu > Contact 15.2 Current operating parameters Press the Information icon to access the “Info” menu from Normal display. “Info” menu. Select the desired information. • Select “Program operation” to see information on a currently running program. •...
Page 132: Event List
(chap. 16.2) the Event list is cleared. 15.4 Technical chamber information Path: Main menu > Device info Chamber name and setup Versions of CPU, I/O module and safety for BINDER controller Service Information on digital and analog inputs for BINDER and outputs and phase angle outputs...
Page 133: Chart Recorder Display
Chart recorder display This view offers graphic representation of the measurement course. Data representation imitates a chart recorder and allows recalling any set of measured data at any point of time taken from the recorded period. 16.1 Views Press the Change view icon to access the pen recorder display. 16.1.1 Show and hide legend Show legend...
Page 134: Show And Hide Specific Indications
16.1.3 Show and hide specific indications Show indications Hide indications Press the Show indications icon to display the indication “Door open” (B2). Indication “Door open” displayed (example: 1 page). 16.1.4 History display History display Press the History display icon to change to the history display. History display.
Page 135 History display: Curve selection Curve selection Press the Curve selection icon to access the “Curve selection” submenu. “Curve selection” submenu. Select the curves to be displayed by checking the checkbox of the corresponding parameter. Press the Confirm icon History display: Search the required instant Search Press the Search icon to access the “Search”...
Page 136 History display: Zoom function Zoom Press the Zoom icon to access the “Zoom” submenu. “Zoom” submenu. Select the zoom factor and press the Confirm icon History display: Show and hide scroll buttons to scroll to an instant Show scroll buttons Hide scroll buttons Press the Show scroll buttons icon to access the “Page selection”...
Page 137: Setting The Parameters
16.2 Setting the parameters This menu allows setting the storage interval, the type of values to be shown and the scaling of the charts. Path: Main menu > Settings > Measurement chart “Measurement chart” submenu. • Select the field “Storage interval” and enter the desired storage interval. Confirm entry with Confirm icon. The available presentation depends on the pre-selected storage rate.
Page 138: Cbf / Cbf-Ul: Humidification System
• Water intake temperature NOT below +5 °C / 41 °F and not exceeding 40 °C / 104 °F. BINDER GmbH is NOT responsible for the water quality provided by the customer. Any problems and malfunctions that might arise following use of water of deviating quality is excluded from liability by BINDER GmbH.
Page 139: Function Of The Dehumidifying System
Options 18.1 APT-COM™ 4 Multi Management Software (option) The chamber is regularly equipped with an Ethernet interface (5) that can connect the BINDER APT-COM™ 4 Multi Management Software. The MAC Address is indicated in the “Device info” controller menu (chap.
Page 140: Quick Sample Access (Option)
When operating a chamber with silicon access ports, both silicon plugs must tightly close the access ports. If the plugs are inserted in a not-gastight manner, or if plugs are missing, CO gas (chamber with control) may escape into the environment. The CO control and the O control (chamber with O control)
Page 141: Interior Socket 230V (Option, Available Via Binder Individual Customized Solutions)
18.4 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions) The interior socket is activated and deactivated in the controller via the switching state of an operation line (chap. 7.3, 9.7.3, 10.6.5). When the interior socket is activated, the notification “Interior socket“ is shown highlighted in black next to the information icon in the screen header in Normal display (chap.
Page 142: Analog Outputs For Temperature And Co (Option)
The interior socket’s voltage is turned off at the chamber controller via operation line (chap. 7.3, 9.7.3, 10.6.5). Turning off the chamber at the main power switch also switches the interior socket voltage-free. Pin 1 (N) Pin 2 (L) Pin 2 (L) Pin 1 (N) Pin 3 (PE) Pin 3 (PE)
Page 143: Data Logger Kits (Option)
BINDER Data Loggers are equipped with a keyboard and a large LCD display, alarm functions and a real- time function. Measurement data are recorded in the Data Logger and can be read out after the measurement via the RS232 interface of the Data Logger.
Page 144: Binder Gas Supply Service - External Bottle Changer For Co
 Make sure that the ambient temperature is ≤ 25 °C / 77 °F when operating electrical devices inside the incubator:.  Check the temperature observation before introducing any cells. 18.8 BINDER Gas Supply Service – External bottle changer for CO or O (option) The external bottle changer permits automatic switching to a second gas cylinder as soon as the first cylinder is empty.
Page 145: Stands
This could happen e.g. while opening or closing the door, cleaning, charging and discharging the chamber. BINDER offers stable, vibration-free stands with castors (2 lockable by brakes) for the safe stacking of two chambers.
Page 146: Reference Measurements
NaHCO buffered media. These test systems are not suitable for calibrating the BINDER sensor system. Recommended procedure: Incubate an empty sample with medium for 1/2 day under the same conditions as the cells. You can •...
Page 147: Measuring Co Directly Via Chemical Indicator Tubes
All the necessary equipment must be supplied by one manufacturer only and in a defined test system. Note: These test systems are not very accurate. A typical accuracy is around 10 % of the full-scale value. These test systems are not suitable for calibrating the BINDER sensor system. 19.1.3...
Page 148: Avoiding Microbial Contamination
Avoiding microbial contamination The main types of microbial contaminants in cell and tissue culture are bacteria, fungi, yeast, mycoplasma, and viruses. This chapter gives an overview of potential sources of contamination and precautions and measures to eliminate them. 20.1 Cells and media Primary cultures from the original tissue •...
Page 149: Chamber Design And Equipment Of The Co Incubator
Condensation in the inner chamber represents a particular risk of contamination. The Permadry™ • system developed by BINDER is an effective and easy way to ensure high humidity (≥95 % r.h.) inside the chamber without any condensation forming on the inner surfaces.
Page 150: Handling The Co Incubator
37 °C / 98.6 °F = ambient temperature 30 °C / 86 °F and less. The chamber should be calibrated / precisely adjusted. • Let BINDER Service adjust the setting of the door heating in reaction to critical ambient conditions. • Regular cleaning, decontamination and sterilization Clean the shelves, glass door, gaskets, and inner chamber weekly (for cleaning see chap.
Page 151: Cleaning, Decontamination / Disinfection, And Sterilization
Cleaning, decontamination / disinfection, and sterilization Clean the chamber after each use in order to prevent potential corrosion damage by ingredients of the charging material. Prior to renewed startup, allow the chamber to completely dry after all cleaning and decontamination measures.
Page 152 Do not use cleaning agents that may cause a hazard due to reaction with components of the device or the charging material. If there is doubt regarding the suitability of cleaning products, please contact BINDER service. We recommend using the neutral cleaning agent Art. No. 1002-0016 for thorough cleaning.
Page 153: Decontamination / Chemical Disinfection Of The Chamber
For chemical disinfection, we recommend using the disinfectant spray Art. No. 1002-0022. Any corrosive damage that may arise following use of other disinfectants is excluded from liability by BINDER GmbH. With every decontamination method, always use adequate personal safety controls.
Page 154: Disinfection Of The Co
∅ NEVER spray the disinfectant directly on the CO sensor.  Wipe the sensor with a lint-free cloth soaked with the disinfectant. Use only the detergents or disinfectants recommended by BINDER. Other products are not approved, since they could in particular damage the CO sensor.
Page 155: Hot-Air Sterilization
Note: in the case when two chambers are directly stacked on top of each other without using the original BINDER stacking stand or the BINDER stacking adapter, the exact maintenance of the incubation set parameters in one of the chambers while carrying out sterilization in the other one cannot be assured.
Page 156: Preparation For A Hot-Air Sterilization
21.3.2 Preparation for a hot-air sterilization Before carrying out the first hot-air sterilization, remove any protective lamination sheet from the inner metal surfaces. When starting a hot-air sterilization, all gas controls automatically deactivate. The safety controller settings are inactive during sterilization. They become functional again following completing or prematurely terminating of the sterilization cycle and/or restart of the chamber at the main power switch.
Page 157 A confirmation prompt is displayed. Make sure that the water pan was emptied and all samples were removed from the chamber and press the Confirm icon to confirm the message. The controller returns to the initial view and the program delay time until program start begins. When the program delay time is over, the hot-air sterilization cycle begins.
Page 158: Prematurely Terminating The Sterilization Cycle
The sterilization program must not be interrupted using the Program pause icon. NOTICE Danger of interrupting the temperature reaction time by pausing the sterilization program during the heating up or holding phase. Ineffective sterilization. ∅ DO NOT interrupt a running sterilization program during the heating up or holding phase of the sterilization cycle.
Page 159: Prematurely Terminating The Sterilization Cycle After More Than 4 Hours, I.e., During The Cooling-Down Phase: Successful Sterilization
NOTICE Danger of interrupting the temperature reaction time by prematurely terminating the sterilization. Ineffective sterilization.  If necessary, repeat the sterilization. The glass door and inner chamber become hot during sterilization. CAUTION Danger of burning by touching hot chamber parts during or after a sterilization. Burns.
Page 160: Completing The Entire Sterilization Cycle
Glass door and inner chamber become hot during sterilization. The glass door handle reaches a temperature of approx. 150 °C / 302 °F. CAUTION Danger of burning through touching the glass door and inner chamber during or after a sterilization. Burns.
Page 161: Maintenance And Service, Troubleshooting, Repair, Testing
For personnel requirements please refer to the Service Manual. • Repair Repair of the chamber can be performed by BINDER Service or by BINDER qualified service partners or technicians, in accordance with the description in the Service Manual. After maintenance, the chamber must be tested prior to resuming operation.
Page 162: Cbf / Cbf-Ul: Replacing Components Of The Humidifying / Dehumidifying System
• Humidification water set (Art. No. 8009-1104, option): 2 m (chap. 4.5.3) • Water container set (Art. No. 8009-1128, option): 10 m (chap. 4.5.4) It is also available by the meter from BINDER. To operate the freshwater supply via tubular bags (standard), a total length of 32 cm is required.
Page 163: Replacing The Co
When using gas with a technical grade of 99.5 %, we recommend changing the fine gas filter once a year. Please consult BINDER Service. When using gases with less pureness, the changing intervals should be shorter.
Page 164: Replacing The Co Sensor
22.4 Replacing the CO sensor • Pull the power plug. CAUTION Risk of injuries by sharp edges on the rear panel. Cuts.  Wear protective gloves when removing or installing the cover on the chamber rear • Remove the cover on the chamber rear The position of the CO sensor behind the cover is shown in Figure 50.
Page 165: Maintenance Intervals, Service
Replace the door gasket only when cold. Otherwise, the door gasket may become damaged. We recommend taking out a maintenance agreement. Please consult BINDER Service. BINDER telephone hotline: +49 (0) 7462 2005 555...
Page 166: Simple Troubleshooting
If there are is a technical fault or shortcoming, take the chamber out of operation and inform BINDER Service. If you are not sure whether there is a technical fault, proceed according to the following list. If you cannot clearly identify an error or there is a technical fault, please contact BINDER Service.
Page 167: Heating
24.4. setpoint by more than the set Controller defective. tolerance range value (more Acknowledge the alarm on the than 10 min.) controller. Contact BINDER service. Semiconductor relay defective Alarm message Acknowledge the alarm on the Temperature controller not “Temp. range”...
Page 168: Gas Cylinder Pressure Too Low
Temperature sensor Transfer the cultures to another reference method.. defective. incubator and contact BINDER Service. 22.6.3 Gas cylinder pressure too low The alarm messages indicate that the pressure in the supply lines of CO (chamber with O...
Page 169: Gas Control
CO exceeds the setpoint by (chap. 1.8). Turn on the chamber again. more than the set tolerance Upon renewed alarm, contact BINDER range value (more than 10 service. min.) Alarm message Acknowledge the alarm on the “CO2 range”...
Page 170 (more than 10 chamber. Turn on the chamber again. min.) (O control option) Upon renewed alarm, contact BINDER Alarm message service. “O range” Acknowledge the alarm on the sensor defective.
Page 171: Sterilization
Doors not closed properly. Close door properly. Humidity set too high. Reduce humidity (chap. 6.8) Chamber placed on very cold Place the chamber on a BINDER stand floor. to increase the distance to the floor. Door gaskets defective. Replace door gaskets...
Page 172: Humidity (Cbf / Cbf-Ul)
3 °C above ambient temperature. Alarm message “Humidity Acknowledge the alarm on the Humidity module defective. system”. controller. Contact BINDER service. Acknowledge the alarm on the Freshwater bag is empty or controller. Fill the freshwater bag contains air. correctly. Alarm message “Freshwater...
Page 173: Open Door
22.7 Sending the chamber back to BINDER GmbH If you return a BINDER product to us for repair or any other reason, we will only accept the product upon presentation of an authorization number (RMA number) that has previously been issued to you. An authorization number will be issued after receiving your complaint either in writing or by telephone prior to your sending the BINDER product back to us.
Page 174: Disposal
The authorization number must be applied to the packaging in such a way that it can be easily recognized or be recorded clearly in the delivery documents. For security reasons we cannot accept a chamber delivery if it does not carry an authorization number. Return address: BINDER GmbH Gänsäcker 16 Abteilung Service 78502 Tuttlingen, Germany Disposal 23.1 Disposal of the transport packing...
Page 175: Decommissioning
(Elektro- und Elektronikgerätegesetz, ElektroG from 20 October 2015, BGBl. I p. 1739) or contact BINDER service who will organize taking back and disposal of the chamber according to the German national law for electrical and electronic equipment (Elektro- und Elektronikgerätegesetz, ElektroG from 20 October 2015, BGBl.
Page 176: Disposal Of The Chamber In The Member States Of The Eu Except For The Federal Republic Of Germany
According to Annex I of Directive 2012/19/EU of the European Parliament and of the Council on waste electrical and electronic equipment (WEEE), BINDER devices are classified as “monitoring and control instruments” (category 9) only intended for professional use“. They must not be disposed of at public collecting points.
Page 177  If your distributor is not able to take back and dispose of the chamber, please contact BINDER service. Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling according to Directive 2012/19/EU. The devices must be free from toxic, infectious or radioactive substances in order to eliminate any health hazards to the employees of the recycling companies.
Page 178: Disposal Of The Chamber In Non-Member States Of The Eu
December 1996 by TÜV CERT). All test equipment used is subject to the administration of measurement and test equipment that is also a constituent part of the BINDER QM DIN EN ISO 9001 systems. They are controlled and calibrated to a DKD-Standard at regular intervals.
Page 179: Definition Of Usable Volume
24.3 Definition of usable volume The usable volume illustrated below is calculated as follows: A, B, C = internal dimensions (W, H, D) a, b, c = wall separation a = 0.1*A b = 0.1*B c = 0.1*C = (A - 2 * a) * (B - 2 * b) * (C - 2 * c) Figure 51: Determination of the usable volume The technical data refers to the defined usable volume.
Page 180 Chamber size Shelves Number of shelves, regular Number of shelves, max. mm x mm 396 x 289 551 x 442 614 x 502 Size of shelf (external) Width x Depth inch x inch 15.59 x 11.38 21.69 x 17.40 24.17 x 19.76 Maximum load per shelf Kg / lbs.
Page 181 22 +/- 3 °C / 71.6 +/- 5.4 °F and a power supply voltage fluctuation of +/-10%. Technical data is determined in accordance to BINDER Factory Standard Part 2:2015 and DIN 12880:2007. All indications are average values, typical for chambers produced in series. We reserve the right to change technical specifications at any time.
Page 182: Equipment And Options (Extract)
Connection kit for CO or N cylinder Water tight internal socket 230 V (max. 3 A), with turn-off switch (available via BINDER INDIVIDUAL Customized Solutions) Analog output temperature and CO 4-20 mA 4-20mA with DIN socket (6 poles), DIN plug included...
Page 183: Accessories And Spare Parts (Extract)
CBF / CBF-UL: Spatial temperature and humidity measurement including certificate 24.6 Accessories and spare parts (extract) BINDER GmbH is responsible for the safety features of the chamber only, provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
Page 184 Chamber size Description Art. no. Divided inner door with cell therapy compartmentation Door hinged right: Variant 8612-0031 8012-2025 • 8 inner doors Door hinged • 4 shelf levels with one partition wall each left: 8012-2028 Partition wall for cell therapy compartmentation 4020-1218 4020-1219 4020-1220...
Page 185 CBF / CBF-UL : Pump head 5013-0204 CBF / CBF-UL : BINDER Pure Aqua Service 8012-0759 CBF / CBF-UL : Cartridge for BINDER Pure Aqua Service 6011-0165 Cleaning kit (neutral cleaning agent, disinfection spray and lint-free disposable 8012-0503 wipes, protective gloves and goggles)
Page 186: Important Conversion Data For Non-Si Units
Spatial temperature and humidity measurement including certificate (27 measuring 8012-0924 points for temperature, 1 measuring point for humidity) For information on components not listed here, please contact BINDER Service. 24.7 Important conversion data for non-SI units 1 ft = 0.305 m 1 m = 100 cm = 3.28 ft = 39.37 inch...
Page 187: Dimensions
24.9 Dimensions 24.9.1 Chamber size 56 (Dimensions in mm) CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 187/199...
Page 188: Chamber Size 170
24.9.2 Chamber size 170 (Dimensions in mm) CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 188/199...
Page 189: Chamber Size 260
24.9.3 Chamber size 260 (Dimensions in mm) CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 189/199...
Page 190: Certificates And Declarations Of Conformity
Certificates and declarations of conformity 25.1 EU Declaration of conformity for CB CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 190/199...
Page 191 CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 191/199...
Page 192: Eu Declaration Of Conformity For Cbf
25.2 EU Declaration of conformity for CBF CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 192/199...
Page 193 CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 193/199...
Page 194: Contamination Clearance Certificate
Contamination clearance certificate 26.1 For chambers located outside the USA and Canada Declaration regarding safety and health Erklärung zur Sicherheit and gesundheitlichen Unbedenklichkeit The German Ordinance on Hazardous Substances (GefStofV), and the regulations regarding safety at the workplace, require that this form be filled out for all products that are returned to us, so that the safety and the health of our employees can be guaranteed.
Page 195 Transportweg/Spediteur: Transport by (means and name of transport company, etc.) Versendung durch (Name Spediteur o.ä.) ___________________________________________________________________________________ Date of dispatch to BINDER GmbH / Tag der Absendung an BINDER GmbH ___________________________________________________________________________________ CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 195/199...
Page 196 We hereby commit ourselves and guarantee that we will indemnify BINDER GmbH for all damages that are a consequence of incomplete or incorrect information provided by us, and that we will exempt BINDER GmbH from eventual damage claims by third parties./ Wir versichern, dass wir gegenüber BINDER für jeden...
Page 197: For Chambers In The Usa And Canada
Please complete this form and the Customer Decontamination Declaration (next 2 pages) and attach the required pictures. E-mail to: IDL_SalesOrderProcessing_USA@binder-world.com After we have received and reviewed the complete information we will decide on the issue of a RMA number. Please be aware that size specifications, voltage specifications as well as performance www.binder-world.us...
Page 198 Customer (End User) Decontamination Declaration Health and Hazard Safety declaration To protect the health of our employees and the safety at the workplace, we require that this form is completed by the user for all products and parts that are returned to us. (Distributors or Service Organizations cannot sign this form) NO RMA number will be issued without a completed form.
Page 199 4.5 Shipping laws and regulations have not been violated. I hereby commit and guarantee that we will indemnify BINDER Inc. for all damages that are a consequence of incomplete or incorrect information provided by us, and that we will indemnify and hold harmless BINDER Inc.

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