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Binder CBF-UL Series Operating Manual

Co2 incubators, co2 incubators with o2 control, co2 incubators with humidity control
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Operating Manual
CB / CB-UL, CBF / CBF-UL (E7)
CO
Incubators
2
CO
Incubators with O
2
CO
Incubators with Humidity control
2
with sterilizable NDIR sensor system for CO
and microprocessor display program controller MB2 with touchscreen
Model
Model version
CB 56
CB56-230V
CB 56-UL
CB56-120V
CB 170
CB170-230V
CB 170
CB170-230V-D
CB 170
CB170-230V-O
CB 170-UL
CB170-120V
CB 170-UL
CB170UL-120V-D
CB 170-UL
CB170-120V-O
CB 260
CB260-230V
CB 260
CB260-230V-O
CB 260-UL
CB260-120V
CB 260-UL
CB260-120V-O
Model
Model version
CBF 170
CBF170-230V
CBF 170
CBF170-230V-O
CBF 170-UL
CBF170-120V
CBF 170-UL
CBF170-120V-O
CBF 260
CBF260-230V
CBF 260
CBF260-230V-O
CBF 260-UL
CBF260-120V
CBF 260-UL
CBF260-120V-O
BINDER GmbH
 Address: Post office box 102, 78502 Tuttlingen, Germany  Phone: +49 7462 2005 0
 Fax: +49 7462 2005 100  Internet: http://www.binder-world.com
 Service Hotline: +49 7462 2005 555
 Service Fax: +49 7462 2005 93 555
 Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3
 Service Hotline Asia Pacific: +852 390 705 04 or +852 390 705 03
Issue 01/2025
Control
2
2
Voltage
Equipment
200-230 V
100-120 V
200-230 V
200-230 V
Access port 30 mm, left
200-230 V
O
control 0-20 vol.-%
2
100-120 V
100-120 V
Access port 30 mm, left
100-120 V
O
control 0-20 vol.-%
2
200-230 V
200-230 V
O
control 0-20 vol.-%
2
100-120 V
100-120 V
O
control 0-20 vol.-%
2
Voltage
Equipment
200-230 V
200-230 V
O
control 0-20 vol.-%
2
100-120 V
100-120 V
O
control 0-20 vol.-%
2
200-230 V
200-230 V
O
control 0-20 vol.-%
2
100-120 V
100-120 V
O
control 0-20 vol.-%
2
Art. no.
9640-0005
9640-0007
9640-0009
9640-0027
9640-0010
9640-0011
9640-0028
9640-0012
9640-0013
9640-0014
9640-0015
9640-0016
Art. no.
9640-0017
9640-0018
9640-0019
9640-0020
9640-0021
9640-0022
9640-0023
9640-0024
Art. No. 7001-0326

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  • Page 1 BINDER GmbH  Address: Post office box 102, 78502 Tuttlingen, Germany  Phone: +49 7462 2005 0  Fax: +49 7462 2005 100  Internet: http://www.binder-world.com  Service Hotline: +49 7462 2005 555  Service Fax: +49 7462 2005 93 555 ...

  • Page 2: Table Of Contents

    General remarks on water supply ................... 43 4.5.1.1 Water supply ........................43 4.5.1.2 Dehumidification ......................43 4.5.1.3 Types of suitable water quality ..................43 4.5.1.4 BINDER Pure Aqua Service (accessory) ................ 44 CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 2/199...
  • Page 3 4.5.2 Water supply via freshwater bag (standard) ................44 4.5.2.1 Scope of delivery ......................44 4.5.2.2 Installation and connection of the freshwater bag (CBF / CBF-UL) ........ 45 4.5.2.3 Refilling the already installed freshwater bag ..............46 4.5.2.4 Replacing the tube connectors for freshwater supply ............. 46 4.5.3 Water supply via prefilled water bottle (humidification water set accessory) ......
  • Page 4 Stopping a running time program ...................... 81 9.2.1 Pausing a running time program ..................... 81 9.2.2 Cancelling a running time program ..................81 Performance after the end of the program ..................81 Creating a new time program ......................82 Program editor: program management ..................... 82 9.5.1 Deleting a time program......................
  • Page 5 18.2 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ..138 18.3 Quick sample access (option) ......................139 18.4 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions) ..... 140 18.5 Analog outputs for temperature and CO (option) ................
  • Page 6 Humidity (CBF / CBF-UL) ..................... 170 22.6.8 Controller ..........................170 22.6.9 Open door ..........................171 22.7 Sending the chamber back to BINDER GmbH ................171 23. DISPOSAL......................172 23.1 Disposal of the transport packing ....................172 23.1.1 Outer chamber packing......................172 23.1.2...
  • Page 7 23.3 Disposal of the chamber in the Federal Republic of Germany ............173 23.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of Germany............................174 23.5 Disposal of the chamber in non-member states of the EU ............. 176 24.

  • Page 8: Safety

    Operating Manual. This Operating Manual is supplemented and updated as needed. Always use the most recent version of the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-to- dateness and validity of this Operating Manual.

  • Page 9: Intellectual Property

    In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual. This operating manual cannot cover all conceivable applications. If you would like additional information, or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or contact us directly, e.g.

  • Page 10: Safety Alert Symbol

    CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury. NOTICE Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product and/or its functions or of a property in its proximity. 1.4.2 Safety alert symbol Use of the safety alert symbol indicates a risk of injury.

  • Page 11: Prohibition Signs

    Prohibition signs Do NOT touch Do NOT spray with water Information to be observed in order to ensure optimum function of the product. 1.4.4 Word message panel structure Type / cause of hazard. Possible consequences.  Instruction how to avoid the hazard: prohibition ...
  • Page 12 Figure 2: Position of additional labels on the CBF / CBF-UL Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER service for these replacements. CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 12/199...

  • Page 13: Type Plate

    78532 Tuttlingen / Germany www.binder-world.com Figure 3: Type plate (example of CB 170 regular chamber) Indications of the type plate (example) Indication Information BINDER Manufacturer: BINDER GmbH CB 170 Model designation CO2 incubator Device name Serial No. 00000000000000 Serial no. of the chamber...

  • Page 14: Ukca Label

    DGUV rule 110-007, issued by the employers’ liability insurance association). BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
  • Page 15 The chamber does not dispose of any measures of explosion protection. DANGER Danger of explosion due to introduction of flammable or explosive substances in the chamber. Serious injury or death from burns and / or explosion pressure. ∅ Do NOT introduce any substance into the chamber which is combustible or explosive at working temperature.

  • Page 16: Precautions When Working With Gases

    WARNING Danger of injury and damages by the chamber tipping over. Injuries and damage to the chamber and the charging material ∅ Do NOT load the chamber door with heavy objects while it is open. The chamber is equipped with a permanent magnet located behind the inner panel of the outer chamber door.
  • Page 17 control: Notes on handling oxygen (O Chamber with O Oxygen (O ) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair.

  • Page 18: Precautions When Handling Gas Cylinders

    DANGER Risk of suffocation through high concentration of N Death by suffocation. ∅ Do NOT set up chambers in non-ventilated recesses.  Ensure technical ventilation measures.  Observe the relevant regulations for handling N  Close the N supply when decommissioning the chamber. 1.10 Precautions when handling gas cylinders General information for safe handling of gas cylinders: •...

  • Page 19: Intended Use

    Such ingredients include in particular acids and halides. Any corrosive damage caused by such ingredients is excluded from liability by BINDER GmbH. The chamber does not dispose of any measures of explosion protection.

  • Page 20: Foreseeable Misuse

    The requirements described in the Operating Manual for installation site and ambient conditions (Chap. 3.4) must be met. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.

  • Page 21: Residual Risks

    • Bypassing or changing protective systems, operation of the chamber without the designated protective systems • Non-observance of messages regarding cleaning and disinfection of the chamber. • Spilling water or cleaning agent on the chamber, water penetrating into the chamber during operation, cleaning or maintenance.

  • Page 22: Operating Instructions

    • Absence of a plausibility check to rule out erroneous inscription of electrical components • Performance of repair work by untrained/insufficiently qualified personnel • Inappropriate repairs which do not meet the quality standard specified by BINDER • Use of replacement parts other than BINDER original replacement parts •...

  • Page 23: Measures To Prevent Accidents

    1.15 Measures to prevent accidents The operator of the chamber must observe the local and national regulations and take precautions to prevent accidents. The manufacturer took the following measures to prevent dangers: • Indications on the type plate See operating manual chap. 1.6. •...

  • Page 24: Cbf / Cbf-Ul : Resistance Of The Humidity Sensor Against Harmful Substances

    1.16 CBF / CBF-UL : Resistance of the humidity sensor against harmful substances The following list of harmful substances refers only to the humidity sensor and does not include any other materials incorporated in the chamber or prohibited substances in relation to explosion protection. Some gases - especially clean gases - do not have any influence on the humidity sensor.

  • Page 25: Chamber Description

    A highly precise, drift-free CO infrared measuring system in combination with the permanent mixture of CO gas through a special proprietary gas mixing head developed by BINDER allows precise and constant CO concentrations for long periods. This creates optimum growth conditions for cultures.

  • Page 26: Chamber Overview

    A resistance humidifying system humidifies the air. For this purpose, use deionized (demineralized) water. The option BINDER Pure Aqua Service allows using the chamber with any degree of water hardness. Controller: The efficient program controller is equipped with a multitude of operating functions, in addition to recorder and alarm functions.

  • Page 27: Cb / Cb Ul Inner Chamber

    (chamber with O control) Pt 100 temperature probe sensor (chamber with O control) Internal socket 230V (max. 3 A) (option, available via BINDER INDIVIDUAL Customized Solutions, chap. 18.4) Perforated shelves, made of stainless steel Glass door handle Measuring access port Inner glass doors Permadry™...

  • Page 28: Cbf / Cbf Ul Inner Chamber

    (chamber with O control) Pt 100 temperature probe sensor (chamber with O control) Humidity sensor Internal socket 230V (max. 3 A) (option, available via BINDER INDIVIDUAL Customized Solutions, chap. 18.4) Perforated shelves Pump Glass door handle Water connection for silicone tube...

  • Page 29: Control Panel On The Rear Of The Chamber

    Control panel on the rear of the chamber (10) (11) (1b) (6b) Figure 8: Rear control panel CB with O control and options (1b) (10) (11) (6a) Figure 9: Rear control panel CB-UL with O control and options (1a) Socket for IEC connector plug / power cable 100-120 V AC (1b) Socket for IEC connector plug / power cable 230 V AC External socket for extra-low voltage supply (option for chamber size 170 / 260, chap.

  • Page 30: Instrument Panel

    Note on second-hand chambers (Ex-Demo-Units): Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly. Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before commissioning the chamber.

  • Page 31: Guidelines For Safe Lifting And Transportation

    • Permissible ambient temperature range during transport: 10 °C / 14 °F to +60 °C / 140 °F. You can order transport packing for moving or shipping purposes from BINDER service. Permissible ambient temperature range during transport: • If the humidifying system has NOT been emptied: +3 °C / 37.4 °F to +60 °C / 140 °F.

  • Page 32: Location Of Installation And Ambient Conditions

    The chambers can be stacked on top of each other (two chambers maximum). For safe stacking that is easy to maintain, use the original BINDER stacking stand (chap. 18.8.2) or the base on castors (chap. 18.8.3).

  • Page 33: Ambient Conditions

    In order to avoid contamination, never place the chamber directly on the floor. NOTICE Danger of overheating due to lack of ventilation. Damage to the chamber. ∅ Do NOT install the chamber in unventilated recesses.  Ensure sufficient ventilation for dispersal of the heat. ...
  • Page 34 DANGER Danger of suffocation and poisoning by high concentration of CO (> 4 Vol.-%). Death by suffocation. ∅ Do NOT set up chambers in non-ventilated recesses.  Ensure technical ventilation measures.  Observe the relevant regulations for handling CO Observe the occupational exposure limit OEL for CO set by the national authorities (formerly maximum permitted workplace concentration).

  • Page 35: Installation And Connections

    Incubator size 260: 40 kg / 88 lb Permadry™ water pan (CB / CB-UL) The Permadry™ system developed by BINDER is an effective and easy to handle system that ensures high humidity inside the chamber without any condensation forming on the inner surfaces. The Permadry™...
  • Page 36 We recommend the BINDER filling water (1000 ml sterile water in water bottles). Art. No. 1007-0327 for the single bottle, Art. No. 8009-1192 for set of 6 bottles •...

  • Page 37: Sensor (Chamber With O 2 Control)

    Connecting the O sensor (chamber with O control) The O sensor is supplied with the chamber in a separate package. Connect or remove the O sensor only when the chamber is turned off. Open the door of the inner chamber and plug the O sensor (F) into the left connection socket located in the upper part of the rear of the inner chamber.

  • Page 38: Connection Of The Co Gas Cylinder

    After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution). 4.4.1 Connection of the CO gas cylinder Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible.

  • Page 39: Connection Of The O Range 10 Up To 90 Vol.-% O 2 )

    Establishing the connection to the chamber Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp. Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (10) DN 6 on the chamber rear, as described in chap.

  • Page 40: Connection Of The N Gas Cylinder (Chamber With O Control)

    The following steps are required: Ensuring the correct O output pressure A gas supply pressure above 2.5 bar / 36 psi will result in chamber damage. Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the chamber.

  • Page 41: Connecting The Gas Hose To The Chamber Rear (For Co )

    The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures. The following steps are required: Ensuring the correct N output pressure A gas supply pressure above 2.5 bar / 36 psi will result in chamber damage. Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the chamber.

  • Page 42: Gas Cylinder Connection Kits (Accessory)

    Otherwise, the quick acting closure socket may leak, and/or it may become impossible to connect the original hose nozzle. In this case, please contact BINDER Service. Now fit the hose nozzle (c) in the quick acting closure socket. To remove the connection, pull the hose nozzle off the quick acting closure socket.

  • Page 43: Cbf / Cbf-Ul: Water Supply And Dehumidification

    1 µS/cm (ultrapure water), may cause acid corrosion due to its low pH). • Tap water that has been treated by the optional water treatment system BINDER Pure Aqua Service (disposable system). A reusable measuring equipment to assess the water quality is included (chap.

  • Page 44: Binder Pure Aqua Service (Accessory)

    4.5.1.4 BINDER Pure Aqua Service (accessory) The BINDER water treatment system (disposable system) is available as an accessory to treat tap water. The lifetime depends on water quality and the amount of treated water used. The measuring equipment to assess the water quality is reusable.

  • Page 45: Installation And Connection Of The Freshwater Bag (Cbf / Cbf-Ul)

    4.5.2.2 Installation and connection of the freshwater bag (CBF / CBF-UL) Figure 18: Water supply via freshwater bag, assembled ⑤ ③ ② (12b) ④ ① (13) (12c) (12a) Figure 19: Installation of the water supply via freshwater bag Pump head (12) consisting of: (13) Pump connector (12a) Pump head cover...

  • Page 46: Refilling The Already Installed Freshwater Bag

    to incubator connection IN from freshwater bag Figure 20: Inserting the silicone tube into the pump head ① ② ③ Attach the silicone tube to the incubator connection IN. Attach the tube connector part with a 3-5 mm nozzle to the 25 cm silicone tube and connect both ④...

  • Page 47: Water Supply Via Prefilled Water Bottle (Humidification Water Set Accessory)

    Scope of delivery Each humidification water set includes all necessary connecting parts: • BINDER-Water-Sample-Kit (sample of one bottle, for 1 week, included in scope of delivery) • BINDER-Water-Set with 10 prefilled one-liter bottles (10 weeks demand) Art.no. 8009-1104 The humidification water set includes: •...

  • Page 48: Installation, Connection And Change Of The Prefilled Water Bottle

    4.5.3.2 Installation, connection and change of the prefilled water bottle Figure 21: Installation of the water supply via prefilled water bottle, assembled ⑤ ③ ④ ② (12b) ① (13) (12c) (12a) Figure 22: Installation of the water supply via prefilled water bottle Pump head (12), consisting of (13) Pump connector...

  • Page 49: Water Supply Via Freshwater Can (Water Container Set Accessory)

    to incubator connection IN from water bottle Figure 23: Inserting the silicone tube into the pump head② ② ③ Attach the silicone tube to the incubator connection IN. Plug the part of the tube connector with 3-5mm nozzle in the 25 cm silicon tube and connect both ④...

  • Page 50: Scope Of Delivery

    DANGER Electrical hazard by water entering the chamber via the rear panel. Deadly electric shock.  The magnetic support adjustable in height (option) may only be attached on the left side of the chamber. ∅ Do NOT place the freshwater can tap on or above the rear panel of the chamber Figure 24: Two CBF / CBF-UL incubators with installed water supply via freshwater can (accessory) 4.5.4.1 Scope of delivery...

  • Page 51: Installation And Connection Of The Freshwater Can (Water Container Set Accessory)

    • 1,2 m PTFE tape (art.no. 1004-0011) for sealing the outlet tap • 12 gas fine filters (sterile filter) (art.no. 6014-0012) • 10 m silicone tube 3/5 mm (art.no. 6008-0311) to the pump or via T-type tube connectors to further chambers •...
  • Page 52 Now you can attach the freshwater can at the chamber and establish the hose connection to the freshwater can. (17) (19a) (19) (18) (23) (12) (20) (21) (22) (23) (23) Figure 27: Installation of the water supply via freshwater can, example with single chamber (17) Freshwater can (22)

  • Page 53: Refilling The Freshwater Can

    Water supply for several chambers: You can branch off the water supply via T-pieces for additional chambers. • Divide the 10 m silicone tube (23) into pieces of a suitable length. Take into account the height of the magnetic can holder •...
  • Page 54 • Use only the original connection cables from BINDER according to the above specification. UL chambers: Use only a UL Listed Power supply cord (UL category ELBZ), SJT 3x14 AWG (2.08 mm²).

  • Page 55: Divided Inner Door With Cell Therapy Compartmentation (Optional)

    Divided inner door with cell therapy compartmentation (optional) With this option, the chambers are equipped with a divided inner door and additional dividers for compartmentation to prevent mixing up of sample vessels. Figure 28: A look inside an incubator with the ”Divided inner door with cell therapy compartmentation” option 4.7.1 Special model CBF 260 “Cell Therapy”...

  • Page 56: Handling And Aligning The Divided Inner Door

    4.7.2 Handling and aligning the divided inner door Rubber profile gasket of individual glass door of the divided inner door Hinge of the set-in divided inner door, with elongated holes to align the inner door Measuring access port Figure 30: Divided inner door (example: CB 170) Pull the handles to open each of the individual glass doors.

  • Page 57: Mounting The Partition Walls For Cell Therapy Compartmentation

    4.7.3 Mounting the partition walls for cell therapy compartmentation Figure 31: Divided inner door with shelves and partition walls (example, chamber sizes 56 and 170) The vertical partition wall is used to compartmentalize the interior of the chamber in connection with the divided inner door.
  • Page 58 Figure 34: Inserting the partition wall Then slide the partition towards the back of the chamber until it clicks into place. Rear Rear Figure 35: Partition wall, size 56, assembled Figure 36: Partition wall, size 170 / 260, assembled Figure 37: Shelf holder with inserted partition walls (example, size 170) CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 58/199...

  • Page 59: Functional Overview Of The Mb2 Chamber Controller

    (CBF / CBF-UL), and specifying special controller functions for each program section. You can enter values or programs directly at the controller or use the APT-COM™ 4 Multi Management Software (accessory) specially developed by BINDER. Operating mode Temperature values...

  • Page 60: Operating Functions In Normal Display

    Operating functions in normal display Current operating mode Text list for information icons Date, time, authorization level of the logged-in user, memory Quick setpoint entry Continue to next screen Back to Normal display Information Program start Setpoint entry Event list Display of active alarms Access to main menu Figure 39: Operating functions of the MB2 controller in normal display (sample values, CBF / CBF-UL with...

  • Page 61: Display Views: Normal Display, Program Display, Chart-Recorder Display

    Display views: Normal display, program display, chart-recorder display Press the Change view icon to toggle between normal display, program display and chart- recorder display. Press the Normal display icon to return from program display and chart recorder display back to Normal display.

  • Page 62: Controller Icons Overview

    Controller icons overview Navigation icons in Normal display Icon Signification Function Main menu Access from Normal display to the main menu Alarm Access from Normal display to the list of active alarms Access from Normal display to the event list Event list Access from Normal display to the setpoint entry menu: setpoint Setpoint setting...
  • Page 63 Functional icons in the chart recorder display Icon Signification Function Show legend Show legend Hide legend Hide legend Switch legend Switch between legend pages Show indication “Door open” (B2) Show indications Hide indications Hide indication “Door open” (B2) Pause chart recorder and change to history display. Data History display recording continues.

  • Page 64: Operating Modes

    Operating modes The MB2 program controller operates in the following operating modes: • Idle mode The controller is not functional, i.e., there is no heating, no CO inlet or O inlet (O control) and no humidification (CBF / CBF-UL). The chamber approximates ambient values. You can activate and deactivate this operating mode with the “Idle mode”...

  • Page 65: Main Menu

    • Access to service data, controller reset to factory settings (chap. 5.5.3) • Available only for users with “Service” and “Admin” authorization level. Full functional range only for BINDER Service (users with “Service” authorization level). “Programs” submenu • Access to the controller’s program functions (chap. 8, 9, 10)

  • Page 66: Settings" Submenu

    5.5.3 “Service” submenu The “Service” submenu is available for users with “Service” or “Admin” authorization level. When logged-in with “Admin” authorization level the user will find information to tell the BINDER Service in service case. Path: Main menu > Service Serial number of the chamber, setup version chap.

  • Page 67: Principle Of Controller Entries

    Principle of controller entries In the selection and entry menus there are icons displayed in the footers which you can use to take over the entry or cancel it. Selection menu (example) Entry menu (example) After completing the settings there are the following possibilities: Press the Confirm icon to take over the entries and exit the menu or continue the menu sequence.

  • Page 68: Performance When Opening The Door

    To reduce odors quickly we recommend heating up the chamber to its nominal temperature for one day and in a well-ventilated location. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.

  • Page 69: Controller Settings Upon Start Up

    Controller settings upon start up The window „Language selection“ enables the language selection, in case that it’s activated in the “Start- up” menu. Afterwards occurs a request of the time zone and the temperature unit. The controller will function in the operating mode, which was active before the last shut-down. It controls temperature, CO (chamber with O control) and humidity (CBF / CBF-UL) in fixed value operating...

  • Page 70: Factory Settings

    Factory settings The chamber is supplied with the following basic preset parameters: Temperature setpoint 37 °C / 98.6 °F • setpoint 5 vol.-% • setpoint (chamber with O control) 21 vol.-% • Humidity setpoint (CBF / CBF-UL) 93 % r.h. •...

  • Page 71: Operating The Duodoor™ Door Lock

    Operating the DuoDoor™ door lock WARNING Magnets can affect the function of pacemakers and implanted defibrillators. A pacemaker can be put into test mode and cause discomfort. A defibrillator may not work anymore.  If you wear such devices, maintain a sufficient safety distance to the chamber door in the area of the door handle.

  • Page 72: Control

    You can deactivate the N pressure alarm in the controller (chap. 7.3). Then also with activated O control no N pressure alarm will be emitted. • Alternating operation in the hyperoxic and hypoxic range or in the range from 20 vol.-% O up to 22 vol.-% O and O...

  • Page 73: Cb / Cb-Ul: Humidity Control Of The Permadry™ System

    If the ambient temperature deviates by more than +/- 5 °C from the values recommended by the manufacturer, the conditions for maximum air humidity with condensation-free inner chamber are no longer guaranteed. Contact BINDER Service for assistance. CB / CB-UL, CBF / CBF-UL (E7) 01/2025...

  • Page 74: Set-Point Entry In "Fixed Value" Operating Mode

    Set-point entry in “Fixed value” operating mode In Fixed value operating mode you can enter set-points for temperature, CO and O (chamber with O control) and humidity (CBF / CBF-UL), and the switching-state of up to 16 special controller functions. All settings made in Fixed value operating mode remain valid until the next manual change.

  • Page 75: Set-Point Entry Through The "Setpoints" Menu

    Set-point entry through the “Setpoints” menu Press the Setpoint setting icon to access the “Setpoint” setting menu from Normal display. “Setpoints” menu. Select “Fixed value operation setpoints” to access the individual parameters. • Select the field “Temperature” and enter the desired temperature setpoint. Setting range: 20 °C up to 60 °C.

  • Page 76: Direct Setpoint Entry Via Normal Display

    • Controller function “N2 pressure alarm off” activates / deactivates the N pressure alarm (chamber with control) • Controller function “Interior socket” activates / deactivates interior socket (option, available via BINDER INDIVIDUAL Customized Solutions) The other controller functions are without function.

  • Page 77: Safety Instructions For Setting High Gas Concentrations

    Use the “Setpoints” menu to configure the controller functions. “Setpoints” menu. “Functions on/off” entry menu (view with chamber with O control with optional interior Select the field “Functions on/off”. socket). Mark / unmark the checkbox to activate / deactivate the desired function and press the Confirm icon Activated controller function: switching status “1”...

  • Page 78: Timer Program: Stopwatch Function

    control: Notes on handling oxygen (O Chamber with O Oxygen (O ) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair.

  • Page 79: Performance During Program Delay Time

    “Program start” menu • In the field “Program type” select “Timer program”. • Select the field “Program duration” and enter the desired program duration. Press the Confirm icon. • Select the field “Program start” and enter the desired start time of the program in the “Program start” entry menu.

  • Page 80: Time Programs

    Time programs The MB2 program controller permits programming time programs with real-time reference. It offers 25 program memory positions with up to 100 program sections each. For each program section you can enter set-points for temperature, CO (chamber with O control), and humidity (CBF / CBF-UL), section duration, type of set-point transition (ramp or step) and the tolerance ranges.

  • Page 81: Performance During Program Delay Time

    9.1.1 Performance during program delay time During the configured program delay time until program start, the controller equilibrates to the current setpoints of Fixed value operation mode. Modifications of these setpoints are effective. When the configured moment for program start is reached, the program delay time ends and the program starts running.

  • Page 82: Creating A New Time Program

    Creating a new time program Path: Main menu > Programs > Time program “Time program” menu: Enter the program name and, if desired, overview of the existing programs. additional program information in the corresponding fields. Select an empty program place. Press the Confirm icon.

  • Page 83: Deleting A Time Program

    Program editor: “Edit program” menu Select the desired function and press the Confirm icon. The program editor offers following options: • Change the program name • Copy program • Replace program: Replacing an new or an existing program with the copied program. This menu point is visible only after a program has been copied.

  • Page 84: Section Editor: Section Management

    Section editor: section management Path: Main menu > Programs > Time program Select the desired program.    Program view. Section view (example: section 1). Select the desired program section There are the following options: (example: section 1)  Select a parameter to enter or modify the according value (chap.

  • Page 85: Add A New Program Section

    9.6.1 Add a new program section Section editor: “Edit section” menu. Select “Create new section” and press the Confirm icon. Then select whether to insert the new section before or after the current section. Press the Confirm icon. The new section opens. 9.6.2 Copy and insert or replace a program section Program view.

  • Page 86: Deleting A Program Section

    Press the Edit icon to open the section editor if you want the current section to be replaced or the copied section to be inserted before or after it Program view. Section view (example: section 1). Select the section to be replaced or before or Press the Edit icon to open the section editor after which the copied section shall be inserted (example: section 3) and press the...

  • Page 87: Value Entry For A Program Section

    Value entry for a program section Path: Main menu > Programs > Time program Select the desired program and section. The section view gives access to all parameters of a program section. You can enter or modify the values. Program name and section number Section duration Type of setpoint transition: ramp or step Special controller functions...

  • Page 88: Set-Point Ramp And Set-Point Step

    9.7.2 Set-point ramp and set-point step You can define the type of transitions for temperature, CO concentration, O concentration (chamber with control) and humidity (CBF / CBF-UL) for each individual program section. “Ramp” mode: Gradual set-points changes The set-point of a given program section functions as the section’s start value. During the section’s duration, each set-point gradually passes to the set-point of the subsequent program section.
  • Page 89 Selecting the setting “Ramp” or “Step” Section view (partial view). In the field “Course” select the desired setting “Ramp” or “Step”. “Ramp” and “Step” mode example (representation of a temperature course) ------------1-------------------------2------------------------3------------4----------5------------ W/°C 40.0 39.5 39.0 38.5 38.0 37.5 37.0 t/min.

  • Page 90: Special Controller Functions

    • Controller function “N2 pressure alarm off” activates / deactivates the N pressure alarm (chamber with control) • Controller function “Interior socket” activates / deactivates interior socket (option, available via BINDER INDIVIDUAL Customized Solutions) The other controller functions are without function.

  • Page 91: Setpoint Entry

    9.7.4 Setpoint entry • Select the field “Temperature” and enter the desired temperature setpoint. Setting range: 20 °C up to 60 °C. Confirm entry with Confirm icon. The controller returns to the section view. • Select the field “CO2” and enter the desired CO setpoint.

  • Page 92: Repeating One Or Several Sections Within A Time Program

    • Select the field “Tolerance band min” and enter the desired lower tolerance band value. Setting range: -99999 to 99999. Confirm entry with Confirm icon. The controller returns to the section view. • Select the field “Tolerance band max” and enter the desired upper tolerance band value. Setting range: -99999 to 99999.

  • Page 93: Saving The Time Program

    9.7.7 Saving the time program Section view. Program view. After the all desired values of the program Press the Confirm icon to take over the section have been configured, press the programming. Confirm icon to take over the programming. The controller changes to the Normal display. The controller changes to the program view.

  • Page 94: Cancelling A Running Week Program

    After completing the settings, press the Confirm icon to take over the entries and exit the menu. The program starts running. If instead you press the Close icon to exit the menu without taking over the entries, the program will not start.

  • Page 95: Program Editor: Program Management

    Program view. For the first section no weekday is specified. Therefore the section is first marked in red and cannot be saved. 10.4 Program editor: program management Path: Main menu > Programs > Week program    “Week program” menu: Program view (example: program 1).

  • Page 96: Deleting A Week Program

    The program editor offers following options: • Change program name. This menu also offers to configure the ramp / step mode setting (chap. 10.6.1). • Copy program • Replace program: Replacing an new or an existing program with the copied program. This menu point is visible only after a section has been copied.

  • Page 97: Section Editor: Section Management

    10.5 Section editor: section management Path: Main menu > Programs > Week program Select the desired program.    Program view. Section view (example: section 1). Select the desired program section There are the following options: (example: section 1) ...

  • Page 98: Add A New Program Section

    10.5.1 Add a new program section Section editor: “Edit section” menu. Program view. Select “Create new section” and press the With a new section no weekday is specified. Confirm icon. Therefore the section is first marked in red and cannot be saved. A new section is always added at the very bottom (example: section 3).

  • Page 99: Deleting A Program Section

    Select “Replace section” to replace the selected section with the copied section Select “Insert section” to additionally add the copied section. Press the Confirm icon. If you selected “Insert section” the sections are automatically arranged in the correct chronological order. Section editor: “Edit section”...

  • Page 100: Weekday

    “Change program name” menu. In the field “Course” select the desired setting “Ramp” or “Step” and press the Confirm icon. 10.6.2 Weekday In the field “Weekday” select the desired weekday. With “Daily” selected, this section will run every day Section view. at the same time.

  • Page 101: Setpoint Entry

    • Controller function “N2 pressure alarm off” activates / deactivates the N pressure alarm (chamber with control) • Controller function “Interior socket” activates / deactivates interior socket (option, available via BINDER INDIVIDUAL Customized Solutions) The other controller functions are without function.

  • Page 102: Notification And Alarm Functions

    Notification and alarm functions 11.1 Notification and alarm messages overview 11.1.1 Notifications Notifications are indicated by information icons displayed in the screen header in Normal display An information icon serves as an indication of a certain condition. If this condition persists, in some cases an alarm will be triggered after a fix or configurable interval. As long as the condition persists, the information icon therefore continues to be displayed also in state of alarm.

  • Page 103: Alarm Messages

    11.1.2 Alarm messages Start after Zero-voltage relay Condition Alarm message condition occurred alarm output Having reached the setpoint, the actual temperature value deviates by more than the set tolerance range value and longer than the set delay After configurable time from the setpoint “Temperature delay time (chap.
  • Page 104 Contact BINDER Service. Chamber with O control: “O2 sensor fault” immediately immediately sensor defective. Contact BINDER Service CBF / CBF-UL: “Humid sensor fault” immediately immediately Humidity sensor defective. Contact BINDER Service Hot air sterilization running “Sterilization”...

  • Page 105: State Of Alarm

    Alarm messages are displayed in the list of active alarms until acknowledging them. They are also shown in the event list. When operating the chamber without CO connection, turn off the corresponding gas control in the “setpoints” menu (chap. 6.7) in order to avoid tolerance range and pressure alarms.

  • Page 106: Activating / Deactivating The Audible Alarm (Alarm Buzzer)

    When the alarm condition has ended, the visual alarm indication is automatically cleared. The alarm is then no longer in the list of active alarms. • Acknowledging after the alarm condition has ended: The buzzer and the visual alarm indication are reset together.
  • Page 107 Alarms following deviations from the tolerance range or door opening If the actual value, after having reached the setpoint, deviates by more than the set tolerance range value and longer than 10 min. from the setpoint or the temperature doesn’t reach the tolerance range within 3 hours from turning on the chamber or closing the door, after the configured interval (e.g., “Temp range alarm delay”) an alarm is triggered.

  • Page 108: Zero-Voltage Relay Alarm Output

    11.6 Zero-voltage relay alarm output The chamber is equipped with a zero-voltage relay output which permits the transmission of some alarm messages to a central monitoring system. The connection is realized as a DIN socket (3) on the chamber rear. A suitable DIN plug is enclosed.

  • Page 109: Temperature Safety Devices

    The user cannot restart the device again. The protective cut-off device is located internally. Only a service specialist can replace it. Therefore, please contact an authorized service provider or BINDER Service. 12.2 Overtemperature safety controller class 3.1 The chambers are regularly equipped with an electronic overtemperature safety controller (temperature safety device class 3.1 according to DIN 12880:2007).

  • Page 110: Safety Controller Modes

    12.2.1 Safety controller modes You can select between “Limit (absolute)” and “Offset (relative)” safety controller mode • Limit: Absolute maximum permitted temperature value This setting offers high safety as a defined temperature limit will not be exceeded. It is important to adapt the safety controller set-point after each modification of the temperature set-point.

  • Page 111: Message And Measures In The State Of Alarm

    Regularly check the safety controller setting for set-point type “Limit” or “Offset” • in Fixed value operating mode according to the entered set-point temperature value • in program mode according to the highest temperature value of the selected temperature program Set the safety controller set-point by approx.

  • Page 112: User Management

    • All passwords can be changed in the “log out” submenu (chap. 13.3). “Service” authorization level • Authorization level only for BINDER service • Extensive authorization for controller operation and configuration, access to service data • The passwords for “Service”, “Admin” and “User” authorization levels can be changed in the “log out”...
  • Page 113 Operation after user login At user login, the authorization level is selected and confirmed by entering the respective password. Following user login, controller operation is available, recognizable by the open-lock icon in the header. The available controller functions correspond to the user’s authorization level. Password protection activated for all levels: operation without user login is locked If passwords have been assigned for all authorization levels, the controller is locked...
  • Page 114 Information window To check the authorization level of the user currently logged-in, select in Normal display the arrow far right in the display header. The information window shows date and time, the controller’s free memory space and under “Authorization” the authorization level of the current user. If passwords have been assigned for all authorization levels, a user without login (password entry) has no authorization.

  • Page 115: Log In

    13.2 Log in Path: Main menu > User > Log in Controller without a user logged-in Selection of user type (example) All selection possibilities are password protected Controller with logged-in user After completing the settings, press the Confirm icon to take over the entries and exit the menu, or press the Close icon to exit the menu without taking over the entries.

  • Page 116: Log Out

    13.3 Log out Path: Main menu > User > Log out User logoff with “Admin” authorization Controller Controller with without a user logged-in user logged-in User logoff with “Admin” authorization Controller Controller with without a user logged-in user logged-in 13.4 User change If the password function has been deactivated (chap.13.5.2) this function is not available.

  • Page 117: Password Assignment And Password Change

    User selection (example) All selection possibilities are password protected Controller with logged-in user 13.5 Password assignment and password change This function is not available for a user logged-in with “User” authorization. 13.5.1 Password change A logged-in user can change the passwords of his current level and of the next lower level(s). Example: A user with “Admin”...
  • Page 118 Selection of the authorization level Enter desired password. If desired, press the Change (example: view with “Admin” authorization) keyboard icon to access other entry windows. In the “Keyboard switch” window you can select different keyboards to enter uppercase and lowercase letters, digits, and special characters.

  • Page 119: Deleting The Password For An Individual Authorization Level

    13.5.2 Deleting the password for an individual authorization level A user logged-in with “Service” or “Admin” authorization can delete the passwords of his current level and of the next lower level(s). To do this no password is entered during a password change. Path: Main menu >...

  • Page 120: New Password Assignment For "Service" Or "Admin" Authorization Level When The Password Function Was Deactivated

    13.5.3 New password assignment for “service” or “admin” authorization level when the password function was deactivated If the password protection for an authorization level has been deactivated, i.e., no password is assigned, no login for this level is possible. Therefore access to this authorization level is available without login. If the password for the “Service”...

  • Page 121: Activation Code

    13.6 Activation code Certain functions of the controller can be unlocked with a previously generated activation code. The activation code enables access to functions available only in the “Service” authorization level by users without a “Service” authorization. Such functions include e.g., adjustment or extended configurations. The activation code is available in authorization levels.

  • Page 122: General Controller Settings

    General controller settings Most of the general settings can be accessed in the “Settings” submenu, which is available for users with “Service” or “Admin” authorization level. It serves to enter date and time, select the language for the controller menus and the desired temperature unit and to configure the controller’s communication functions.
  • Page 123 Or later: Path: Main menu > Settings > Date and time “Date and time” submenu. “Date / time” entry menu. Select the field “Date / time”. Enter date and time and press the Confirm icon. “Date and time” submenu. “Date and time” submenu. In the field “Daylight saving time switch”...

  • Page 124: Selecting The Temperature Unit

    14.3 Selecting the temperature unit Following start-up of the chamber: Or later: Path: Main menu > Settings > Chamber Select the desired temperature unit and press the Confirm icon. Change of the temperature unit between °C and °F. If the unit is changed, all values are converted accordingly C = degree Celsius 0 °C = 31°F...

  • Page 125: Touchscreen Calibration

    • Select the field “Brightness”. Move the grey slide to the left or right to define the brightness of the display • left = darker (minimum value: 0) • right = brighter (maximum value: 100) Press the Confirm icon. • Select the field “Wait time for screen saver” and enter the desired waiting time for the screen saver in seconds.

  • Page 126: Network And Communication

    This menu allows to configure the communication parameters of the RS485 interface. The device address is required to recognize chambers with this interface type in a network, e.g. when connecting it to the optional BINDER APT-COM™ 4 Multi Management Software (chap. 18.1). In this case do not change the other parameters.

  • Page 127: Ethernet

    14.5.2 Ethernet 14.5.2.1 Configuration Path: Main menu > Settings > Ethernet “Ethernet” submenu. • In the field “IP address assignment” select the desired setting “Automatic (DHCP)“ “Manual“. With selection “Manual” you can enter the IP- address, the subnet mask and the standard gateway manually.

  • Page 128: Display Of Mac Address

    14.5.2.2 Display of MAC address Path: Main menu > Device info > Ethernet “Ethernet” submenu (example). 14.5.3 E-Mail As soon as an alarm was triggered, an e-mail is sent to the configured e-mail address. Path: Main menu > Settings > Email E-mail address entry: “Email”...

  • Page 129: Usb Menu: Data Transfer Via Usb Interface

    • In the field “Authentication” select the desired setting “None” or “SMTP” auth“. With the setting “SMTP auth”, you can enter a password under “Email password”. • Select the field “Email user name” and enter the desired user name. Confirm entry with Confirm icon. •...

  • Page 130: Controller Firmware Update

    Function Explanation Log-out USB stick Log-out USB stick bevor pulling it Export new chart recorder data (*.DAT) Export chart recorder data, which have been added since last export, in .dat format Export all chart recorder data (*.DAT) Export all chart recorder data in .dat format Export all chart recorder data (*.csv) Export all chart recorder data in .csv format Import configuration and programs...

  • Page 131: General Information

    General information 15.1 Service contact page Path: Main menu > Contact 15.2 Current operating parameters Press the Information icon to access the “Info” menu from Normal display. “Info” menu. Select the desired information. • Select “Program operation” to see information on a currently running program. •...

  • Page 132: Event List

    (chap. 16.2) the Event list is cleared. 15.4 Technical chamber information Path: Main menu > Device info Chamber name and setup Versions of CPU, I/O module and safety for BINDER controller Service Information on digital and analog inputs for BINDER and outputs and phase angle outputs...

  • Page 133: Chart Recorder Display

    Chart recorder display This view offers graphic representation of the measurement course. Data representation imitates a chart recorder and allows recalling any set of measured data at any point of time taken from the recorded period. 16.1 Views Press the Change view icon to access the pen recorder display. 16.1.1 Show and hide legend Show legend...

  • Page 134: Show And Hide Specific Indications

    16.1.3 Show and hide specific indications Show indications Hide indications Press the Show indications icon to display the indication “Door open” (B2). Indication “Door open” displayed (example: 1 page). 16.1.4 History display History display Press the History display icon to change to the history display. History display.
  • Page 135 History display: Search the required instant Search Press the Search icon to access the “Search” submenu. “Search” submenu. Select the required instant by entering its date and time and press the Confirm icon History display: Zoom function Zoom Press the Zoom icon to access the “Zoom” submenu. “Zoom”...

  • Page 136: Setting The Parameters

    16.2 Setting the parameters This menu allows setting the storage interval, the type of values to be shown and the scaling of the charts. Path: Main menu > Settings > Measurement chart “Measurement chart” submenu. • Select the field “Storage interval” and enter the desired storage interval. Confirm entry with Confirm icon. The available presentation depends on the pre-selected storage rate.

  • Page 137: Cbf / Cbf-Ul: Humidification System

    • Water intake temperature NOT below +5 °C / 41 °F and not exceeding 40 °C / 104 °F. BINDER GmbH is NOT responsible for the water quality provided by the customer. Any problems and malfunctions that might arise following use of water of deviating quality is excluded from liability by BINDER GmbH.

  • Page 138: Function Of The Dehumidifying System

    Options and accessories 18.1 APT-COM™ 4 Multi Management Software (accessory) The chamber is regularly equipped with an Ethernet interface (5) that can connect the BINDER APT-COM™ 4 Multi Management Software. The MAC Address is indicated in the “Device info” controller menu (chap.

  • Page 139: Quick Sample Access (Option)

    When operating a chamber with silicon access ports, both silicon plugs must tightly close the access ports. If the plugs are inserted in a not-gastight manner, or if plugs are missing, CO gas (chamber with control) may escape into the environment. The CO control and the O control (chamber with O control)

  • Page 140: Interior Socket 230V (Option, Available Via Binder Individual Customized Solutions)

    18.4 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions) The interior socket is activated and deactivated in the controller via the switching state of a special controller function (chap. 7.3, 9.7.3, 10.6.5). When the interior socket is activated, the notification “Interior socket“ is shown highlighted in black next to the information icon in the screen header in Normal display (chap.

  • Page 141: Analog Outputs For Temperature And Co (Option)

    The interior socket’s voltage is turned off at the chamber controller via a special controller function (chap. 7.3, 9.7.3, 10.6.5). Turning off the chamber at the main power switch also switches the interior socket voltage-free. Pin 1 (N) Pin 2 (L) Pin 2 (L) Pin 1 (N) Pin 3 (PE)

  • Page 142: Access Port For Extra-Low Voltage (Option)

    18.6 Access port for extra-low voltage (option) The access port (B) (8-pin) for extra-low voltage (ELV) consists of a LEMO socket (which can be covered) and a LEMO connector. It is suitable to connect two electrical devices, one inside and one outside the incubator.

  • Page 143: Binder Gas Supply Service - External Bottle Changer For Co Or O (Option)

    We recommend not stacking the incubators directly on top of one another in order to avoid transmission of shocks and vibrations from one chamber to the other. This could happen e.g. while opening or closing the door, cleaning, charging and discharging the chamber. BINDER offers a flat stacking adapter for the safe stacking of two BINDER CO -incubators.

  • Page 144: Base On Castors (Accessory)

    18.8.3 Base on castors (accessory) In order to obtain easy access to the chamber, for safe placement and leveling of a BINDER CO₂ incubator, and to avoid contamination of the chamber caused by soil pollution, BINDER recommends using the base on castors.

  • Page 145: Co Reference Measuring

    NaHCO buffered media. These test systems are not suitable for calibrating the BINDER sensor system. Recommended procedure: Incubate an empty sample with medium for 1/2 day under the same conditions as the cells. You can •...

  • Page 146: Measuring Co Directly Via Chemical Indicator Tubes

    All the necessary equipment must be supplied by one manufacturer only and in a defined test system. Note: These test systems are not very accurate. A typical accuracy is around 10 % of the full-scale value. These test systems are not suitable for calibrating the BINDER sensor system. 19.1.3...

  • Page 147: Laboratory Conditions / Equipment Around The Incubator

    20.2 Laboratory conditions / equipment around the incubator Possible sources of contamination in the cell culture lab are airborne germs, lab equipment, building features, and the lab personnel. Keep pipettes and instruments sterile after autoclaving. • Bio safety cabinets (laminar air flow) should have a minimum of items apart from aspirator tube and •...

  • Page 148: Handling The Co Incubator

    • Condensation in the inner chamber represents a particular risk of contamination. The Permadry™ system (CB / CB-UL) developed by BINDER is an effective and easy way to ensure high humidity (≥95 % r.h.) inside the chamber without any condensation forming on the inner surfaces.

  • Page 149: Cleaning, Decontamination / Disinfection, And Sterilization

    37 °C / 98.6 °F = ambient temperature 30 °C / 86 °F and less. The chamber should be calibrated / precisely adjusted. • Let BINDER Service adjust the setting of the door heating in reaction to critical ambient conditions. • Regular cleaning, decontamination and sterilization Clean the shelves, glass door, gaskets, and inner chamber weekly (for cleaning see chap.

  • Page 150: Cleaning

    Do NOT use a neutral cleaning agent on zinc coated surfaces. Do not use cleaning agents that may cause a hazard due to reaction with components of the device or the charging material. If there is doubt regarding the suitability of cleaning products, please contact BINDER service.

  • Page 151: Decontamination / Chemical Disinfection Of The Chamber

    ∅ NEVER spray any cleaning detergents directly on the CO sensor.  Wipe the sensor with a lint-free cloth soaked with cleaning agent. Use only the detergents or disinfectants recommended by BINDER. Other products are not approved, since they could in particular damage the CO sensor.
  • Page 152 For chemical disinfection, we recommend using the disinfectant spray Art. No. 1002-0022. Any corrosive damage that may arise following use of other disinfectants is excluded from liability by BINDER GmbH. With every decontamination method, always use adequate personal safety controls.

  • Page 153: Disinfection Of The Co

    ∅ NEVER spray the disinfectant directly on the CO sensor.  Wipe the sensor with a lint-free cloth soaked with the disinfectant. Use only the detergents or disinfectants recommended by BINDER. Other products are not approved, since they could in particular damage the CO sensor.

  • Page 154: Preparation For A Hot-Air Sterilization

    Note: in the case when two chambers are directly stacked on top of each other without using the original BINDER stacking stand or the BINDER stacking adapter, the exact maintenance of the incubation set parameters in one of the chambers while carrying out sterilization in the other one cannot be assured.

  • Page 155: Starting The Sterilization Cycle And Running The Hot-Air Sterilization

    Water pans (CB / CB-UL) and shelves must be inside the chamber. • Close the inner glass door and the outer chamber door. • Before starting a hot-air sterilization, the entire interior must be clean and dry. No residue of e.g., water, medium or plastic must remain inside the chamber.
  • Page 156 Normal display with alarm message “Sterilization”. The sterilization is running. The zero-voltage relay alarm output and the buzzer are not activated. Opening the outer door leads to prematurely terminating the running sterilization cycle. If this happens during the heating up or holding phase (during 4 hours after starting the sterilization cycle), sterilization will be ineffective (chap.

  • Page 157: Prematurely Terminating The Sterilization Cycle

    21.3.4 Prematurely terminating the sterilization cycle Three events lead to terminating the sterilization cycle prematurely: • Cancelling the program via the controller menu To prematurely terminate the sterilization cycle via the controller menu, press the Program cancelling icon and confirm the confirmation prompt with the Confirm icon (chap. 9.2.2). •...

  • Page 158: Prematurely Terminating The Sterilization Cycle After More Than 4 Hours, I.e., During The Cooling-Down Phase: Successful Sterilization

    21.3.4.2 Prematurely terminating the sterilization cycle after more than 4 hours, i.e., during the cooling-down phase: successful sterilization The duration of the entire sterilization is 10 hours. If you want to shorten the sterilization procedure in order to save time, you can prematurely terminate it during the cooling-down phase, i.e. no sooner than after 4 hours.

  • Page 159: Completing The Entire Sterilization Cycle

    For personnel requirements please refer to the Service Manual. • Repair Repair of the chamber can be performed by BINDER Service or by BINDER qualified service partners or technicians, in accordance with the description in the Service Manual. After maintenance, the chamber must be tested prior to resuming operation.

  • Page 160: Cbf / Cbf-Ul: Replacing Components Of The Humidifying / Dehumidifying System

    • Tubular bag set with 12 tubular bags (art. no. 8012-2255, accessory): 12 pc. 25 cm silicone tube 3/5 mm (art. no. 6008-0320 (chap. 4.5.2) • BINDER water set (art. no. 8009-1104, accessory): 3 pc. 25 cm silicone tube 3/5 mm for the pump (art. no. 6008-0320 (chap. 4.5.3) •...

  • Page 161: Replacing The Co

    When using gas with a technical grade of 99.5 %, we recommend changing the fine gas filter once a year. Please consult BINDER Service. When using gases with less pureness, the changing intervals should be shorter.

  • Page 162: Replacing The Co Sensor

    22.4 Replacing the CO sensor • Pull the power plug. CAUTION Risk of injuries by sharp edges on the rear panel. Cuts.  Wear protective gloves when removing or installing the cover on the chamber rear • Remove the cover on the chamber rear The position of the CO sensor behind the cover is shown in Figure 54.

  • Page 163: Maintenance Intervals, Service

    Replace the door gasket only when cold. Otherwise, the door gasket may become damaged. We recommend taking out a maintenance agreement. Please consult BINDER Service. BINDER telephone hotline: +49 (0) 7462 2005 555...

  • Page 164: Simple Troubleshooting

    If there are is a technical fault or shortcoming, take the chamber out of operation and inform BINDER Service. If you are not sure whether there is a technical fault, proceed according to the following list. If you cannot clearly identify an error or there is a technical fault, please contact BINDER Service.

  • Page 165: Heating

    24.4. setpoint by more than the set Controller defective. tolerance range value (more Acknowledge the alarm on the than 10 min.) controller. Contact BINDER service. Semiconductor relay defective Alarm message Acknowledge the alarm on the Temperature controller not “Temp. range”...

  • Page 166: Gas Cylinder Pressure Too Low

    Temperature sensor Transfer the cultures to another reference method.. defective. incubator and contact BINDER Service. 22.6.3 Gas cylinder pressure too low The alarm messages indicate that the pressure in the supply lines of CO (chamber with O...

  • Page 167: Gas Control

    CO exceeds the setpoint by (chap. 1.9). Turn on the chamber again. more than the set tolerance Upon renewed alarm, contact BINDER range value (more than 10 service. min.) Alarm message Acknowledge the alarm on the “CO2 range”...
  • Page 168 Acknowledge the alarm on the sensor defective. controller. Contact BINDER Service. Recovery time (up to 5 vol.-% ) after doors were open for sensor system defective. Contact BINDER Service. 2 minutes is < 2 minutes Too low CO concentration. Acknowledge the alarm on the controller.

  • Page 169: Sterilization

    Doors not closed properly. Close door properly. Humidity set too high. Reduce humidity (chap. 6.8) Chamber placed on very cold Place the chamber on a BINDER stand floor. to increase the distance to the floor. Door gaskets defective. Replace door gaskets...

  • Page 170: Humidity (Cbf / Cbf-Ul)

    3 °C above ambient temperature. Alarm message “Humidity Acknowledge the alarm on the Humidity module defective. system”. controller. Contact BINDER service. Acknowledge the alarm on the Freshwater bag is empty or controller. Fill the freshwater bag contains air. correctly. Alarm message “Freshwater...

  • Page 171: Open Door

    22.7 Sending the chamber back to BINDER GmbH If you return a BINDER product to us for repair or any other reason, we will only accept the product upon presentation of an authorization number (RMA number) that has previously been issued to you. An authorization number will be issued after receiving your complaint either in writing or by telephone prior to your sending the BINDER product back to us.

  • Page 172: Disposal

    The authorization number must be applied to the packaging in such a way that it can be easily recognized or be recorded clearly in the delivery documents. For security reasons we cannot accept a chamber delivery if it does not carry an authorization number. Return address: BINDER GmbH Gänsäcker 16 Abteilung Service 78502 Tuttlingen, Germany Disposal 23.1 Disposal of the transport packing...

  • Page 173: Decommissioning

    (Elektro- und Elektronikgerätegesetz, ElektroG from 20 October 2015, BGBl. I p. 1739) or contact BINDER service who will organize taking back and disposal of the chamber according to the German national law for electrical and electronic equipment (Elektro- und Elektronikgerätegesetz, ElektroG from 20 October 2015, BGBl.

  • Page 174: Disposal Of The Chamber In The Member States Of The Eu Except For The Federal Republic Of Germany

    According to Annex I of Directive 2012/19/EU of the European Parliament and of the Council on waste electrical and electronic equipment (WEEE), BINDER devices are classified as “monitoring and control instruments” (category 9) only intended for professional use“. They must not be disposed of at public collecting points.
  • Page 175  If your distributor is not able to take back and dispose of the chamber, please contact BINDER service. Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling according to Directive 2012/19/EU. The devices must be free from toxic, infectious or radioactive substances in order to eliminate any health hazards to the employees of the recycling companies.

  • Page 176: Disposal Of The Chamber In Non-Member States Of The Eu

    December 1996 by TÜV CERT). All test equipment used is subject to the administration of measurement and test equipment that is also a constituent part of the BINDER QM DIN EN ISO 9001 systems. They are controlled and calibrated to a DKD-Standard at regular intervals.

  • Page 177: Definition Of Usable Volume

    24.3 Definition of usable volume The usable volume illustrated below is calculated as follows: A, B, C = internal dimensions (W, H, D) a, b, c = wall separation a = 0.1*A b = 0.1*B c = 0.1*C = (A - 2 * a) * (B - 2 * b) * (C - 2 * c) Figure 55: Determination of the usable volume The technical data refers to the defined usable volume.
  • Page 178 Chamber size Shelves Number of shelves, regular Number of shelves, max. mm x mm 396 x 289 551 x 442 614 x 502 Size of shelf (external) Width x Depth inch x inch 15.59 x 11.38 21.69 x 17.40 24.17 x 19.76 Maximum load per shelf Kg / lbs.
  • Page 179 22 +/- 3 °C / 71.6 +/- 5.4 °F and a power supply voltage fluctuation of +/-10%. Technical data is determined in accordance to BINDER Factory Standard Part 2:2015 and DIN 12880:2007. All indications are average values, typical for chambers produced in series. We reserve the right to change technical specifications at any time.

  • Page 180: Equipment, Options, And Accessories (Extract)

    Connection kit for CO or N cylinder Water tight internal socket 230 V (max. 3 A), with turn-off switch (available via BINDER INDIVIDUAL Customized Solutions) Analog output temperature and CO 4-20 mA 4-20mA with DIN socket (6 poles), DIN plug included...

  • Page 181: Spare Parts, Options, And Accessories (Extract)

    CBF / CBF-UL: Spatial temperature and humidity measurement including certificate 24.6 Spare parts, options, and accessories (extract) BINDER GmbH is responsible for the safety features of the chamber only, provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
  • Page 182 Chamber size Description Art. no. Partition wall for cell therapy compartmentation 4020-1218 4020-1219 4020-1220 Divided shelves (1 level) for divided inner door, stainless 6004-0226 6004-0227 steel Extra-deep shelf for divided inner door, stainless steel 6004-0120 Door gasket for outer chamber door 6005-0294 6005-0275 6005-0296...
  • Page 183 CB: BINDER filling water for water pan (1 bottle with 1 l of sterile water) 1007-0327 CB: BINDER filling water for water pan (set of 6 bottles with 1 l of sterile water each) 8009-1192 Cleaning kit (neutral cleaning agent, disinfection spray and lint-free disposable...

  • Page 184: Important Conversion Data For Non-Si Units

    Spatial temperature and humidity measurement including certificate (27 measuring 8012-0924 points for temperature, 1 measuring point for humidity) For information on components not listed here, please contact BINDER Service. 24.7 Important conversion data for non-SI units 1 ft = 0.305 m 1 m = 100 cm = 3.28 ft = 39.37 inch...

  • Page 185: Dimensions

    24.9 Dimensions 24.9.1 Chamber size 56 (Dimensions in mm) CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 185/199...

  • Page 186: Chamber Size 170

    24.9.2 Chamber size 170 (Dimensions in mm) CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 186/199...

  • Page 187: Chamber Size 260

    24.9.3 Chamber size 260 (Dimensions in mm) CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 187/199...

  • Page 188: Certificates And Declarations Of Conformity

    Certificates and declarations of conformity 25.1 EU Declaration of conformity for CB CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 188/199...
  • Page 189 CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 189/199...

  • Page 190: Eu Declaration Of Conformity For Cbf

    25.2 EU Declaration of conformity for CBF CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 190/199...
  • Page 191 CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 191/199...

  • Page 192: Ukca Declaration Of Conformity For Cb

    25.3 UKCA Declaration of Conformity for CB CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 192/199...

  • Page 193: Ukca Declaration Of Conformity For Cbf

    25.4 UKCA Declaration of Conformity for CBF CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 193/199...

  • Page 194: Contamination Clearance Certificate

    Contamination clearance certificate 26.1 For chambers located outside the USA and Canada Declaration regarding safety and health Erklärung zur Sicherheit and gesundheitlichen Unbedenklichkeit The German Ordinance on Hazardous Substances (GefStofV), and the regulations regarding safety at the workplace, require that this form be filled out for all products that are returned to us, so that the safety and the health of our employees can be guaranteed.
  • Page 195 Transportweg/Spediteur: Transport by (means and name of transport company, etc.) Versendung durch (Name Spediteur o.ä.) ___________________________________________________________________________________ Date of dispatch to BINDER GmbH / Tag der Absendung an BINDER GmbH ___________________________________________________________________________________ CB / CB-UL, CBF / CBF-UL (E7) 01/2025 Page 195/199...
  • Page 196 We hereby commit ourselves and guarantee that we will indemnify BINDER GmbH for all damages that are a consequence of incomplete or incorrect information provided by us, and that we will exempt BINDER GmbH from eventual damage claims by third parties./ Wir versichern, dass wir gegenüber BINDER für jeden...

  • Page 197: For Chambers In The Usa And Canada

    Please complete this form and the Customer Decontamination Declaration (next 2 pages) and attach the required pictures. E-mail to: IDL_SalesOrderProcessing_USA@binder-world.com After we have received and reviewed the complete information we will decide on the issue of a RMA number. Please be aware that size specifications, voltage specifications as well as performance www.binder-world.us...
  • Page 198 Customer (End User) Decontamination Declaration Health and Hazard Safety declaration To protect the health of our employees and the safety at the workplace, we require that this form is completed by the user for all products and parts that are returned to us. (Distributors or Service Organizations cannot sign this form) NO RMA number will be issued without a completed form.
  • Page 199 4.5 Shipping laws and regulations have not been violated. I hereby commit and guarantee that we will indemnify BINDER Inc. for all damages that are a consequence of incomplete or incorrect information provided by us, and that we will indemnify and hold harmless BINDER Inc.

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