Binder CB 60-UL Operating Manual
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Binder CB 60-UL Operating Manual

Cb (e6.1) co2 - incubators co2 – incubators with o2 control with sterilizable ndir sensor system for co2 and microprocessor controller t4.12
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Operating Manual
CB (E6.1)
CO
- Incubators
2
CO
– Incubators with O
2
with sterilizable NDIR sensor system for CO
and microprocessor controller T4.12
Model
Voltage
CB 60
230 V
CB 60-UL
100-120V
CB 60
230 V
CB 60-UL
100-120V
CB 60
230 V
CB 60-UL
100-120V
CB 60
230 V
CB 60-UL
100-120V
CB 160 (E6)
230 V
CB 160-UL
100-120V
CB 160 (E6)
230 V
CB 160-UL
100-120V
CB 160
230 V
CB 160-UL
100-120V
CB 160
230 V
CB 160-UL
100-120V
CB 220
230 V
CB 220-UL
100-120V
CB 220
230 V
CB 220-UL
100-120V
CB 220
230 V
CB 220-UL
100-120V
CB 220
230 V
CB 220-UL
100-120V
BINDER GmbH
 Address: Post office box 102, 78502 Tuttlingen, Germany  Phone: +49 7462 2005 0
 Fax: +49 7462 2005 100  Internet: http://www.binder-world.com
 E-mail: info@binder-world.com  Service Hotline: +49 7462 2005 555
 Service Fax: +49 7462 2005 93 555  Service E-Mail: service@binder-world.com
 Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3
 Service Hotline Asia Pacific: +852 390 705 04 or +852 390 705 03
 Service Hotline Russia and CIS: +7 495 988 15 16
Issue 07/2017
2
Equipment
O
control
2
O
control
2
Divided inner door
Divided inner door
O
control
Divided inner door
2
O
control
Divided inner door
2
O
control
2
O
control
2
Divided inner door
Divided inner door
O
control
Divided inner door
2
O
control
Divided inner door
2
O
control
2
O
control
2
Divided inner door
Divided inner door
O
control
Divided inner door
2
O
control
Divided inner door
2
control
2
Art. No.
9040-0088, 9140-0088
9040-0089, 9140-0089
9040-0090, 9140-0090
9040-0091, 9140-0091
9040-0104, 9140-0104
9040-0105, 9140-0105
9040-0106, 9140-0106
9040-0107, 9140-0107
9040-0092, 9140-0092
9040-0093, 9140-0093
9040-0094, 9140-0094
9040-0095, 9140-0095
9040-0100, 9140-0100
9040-0101, 9140-0101
9040-0102, 9140-0102
9040-0103, 9140-0103
9040-0096, 9140-0096
9040-0097, 9140-0097
9040-0098, 9140-0098
9040-0099, 9140-0099
9040-0108, 9140-0108
9040-0109, 9140-0109
9040-0110, 9140-0110
9040-0111, 9140-0111
Art. No. 7001-0333

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Summary of Contents for Binder CB 60-UL

  • Page 1  Fax: +49 7462 2005 100  Internet: http://www.binder-world.com  E-mail: info@binder-world.com  Service Hotline: +49 7462 2005 555  Service Fax: +49 7462 2005 93 555  Service E-Mail: service@binder-world.com  Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3 ...

  • Page 2: Table Of Contents

    Contents SAFETY ........................6 Legal considerations ........................... 6 Structure of the safety instructions ...................... 6 1.2.1 Signal word panel ........................6 1.2.2 Safety alert symbol ........................7 1.2.3 Pictograms ..........................7 1.2.4 Word message panel structure ....................8 Localization / position of safety labels on the chamber ..............8 Type plate ............................
  • Page 3 Humidity control of the Permadry™ system ..................45 Turning on / off the interior socket voltage (with optional interior socket) ......... 46 Performance during and after power failure ..................46 Information ............................47 SETPOINT ENTRY ....................48 Setting ranges ........................... 48 Note when setting high gas concentrations ..................
  • Page 4 15.4 Interior socket 230V (option) ......................92 15.5 Analog outputs for temperature and CO (option) ................94 15.6 Access port for extra-low voltage (option) ..................94 15.7 BINDER Gas Supply Service – External bottle changer for CO or O (option) ......95 15.8 Stands ............................... 96 15.8.1...
  • Page 5 21. TROUBLESHOOTING ..................121 21.1 General............................121 21.2 Heating ............................121 21.3 Gas cylinder pressure too low ......................123 21.4 Gas concentration ........................... 124 21.5 Sterilization ............................126 21.6 Humidity ............................126 21.7 Controller ............................127 21.8 Open door ............................127 22.

  • Page 6: Safety

    Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use and safety during operation and maintenance. In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual.

  • Page 7: Safety Alert Symbol

    WARNING Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irreversible) injury. CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury. CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product and/or its functions or of a property in its proximity.

  • Page 8: Word Message Panel Structure

    Mandatory action signs Mandatory regulation Lift with several persons Read operating Disconnect the power instructions plug Wear safety goggles Wear protective gloves Environment protection Prohibition signs Do NOT touch Do NOT spray with Do NOT climb water Information to be observed in order to ensure optimum function of the product. 1.2.4 Word message panel structure Type / cause of hazard.
  • Page 9 -Incubator CB -Incubator CB-UL Figure 1: Position of labels on the chamber Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER service for these replacements. CB (E6.1) 07/2017 Page 9/145...

  • Page 10: Type Plate

    E6.1 78532 Tuttlingen / Germany www.binder-world.com Figure 2: Type plate (example of CB 160 regular chamber) Indications of the type plate (example) Information BINDER Manufacturer: BINDER GmbH CB 160 Model designation CO2 incubator Device name Serial No. 00000000000000 Serial no. of the chamber...

  • Page 11: General Safety Instructions On Installing And Operating The Co Incubator

    BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory guidelines issued by the employers’ liability insurance association) (for Germany). BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.

  • Page 12: Precautions When Working With Gases

    During and after a sterilization the temperature of the inner surfaces almost equals the set-point. CAUTION The inner doors, the inner door and glass door handles, and the inner chamber will become hot during a sterilization. Danger of burning. ∅ Do NOT touch the inner doors, the inner door and glass door handles, and the inner surfaces after a sterilization.
  • Page 13 Chamber with O control: Notes on handling oxygen (O Oxygen (O ) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O is heavier than air and may accumulate in low-lying areas.

  • Page 14: Precautions When Handling Gas Cylinders

    Precautions when handling gas cylinders General information for safe handling of gas cylinders: • Store and use gas cylinders only in well-ventilated locations. • Open the gas cylinder valve slowly to avoid pressure surges. • Secure gas cylinders during storage and use against falling (chaining). •...

  • Page 15: Intended Use

    Due to the special demands of the Medical Device Directive (MDD), these chambers are not qualified for sterilization of medical devices as defined by the directive 93/42/EWG. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.

  • Page 16: Chamber Description

    A highly precise, drift-free CO infrared measuring system in combination with the permanent mixture of gas through a special proprietary gas mixing head developed by BINDER allows precise and constant CO concentrations for long periods. This creates optimum growth conditions for cultures.

  • Page 17: Chamber Overview

    Temperature range: 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F range: 0 vol.-% up to 20 vol.-%) range (chamber with O control): 0.2 vol.-% up to 20 vol.-% (hypoxic control range) or 10 Vol.-% up to 95 Vol.-% (alternative control range) Chamber overview Instrument panel with microprocessor...

  • Page 18: Inner Chamber

    Inner chamber (D2) Figure 5: CB 160 with O control and options CB (E6.1) 07/2017 Page 18/145...
  • Page 19 (D2) control and options Figure 6: CB 160 / CB 220 with O Instrument panel with microprocessor controller T4.12, indicating temperature and CO as well as (chamber with O control)” Connection socket for extra-low voltage supply (option, chap. 15.6) sensor Gas mixing head CO (D2) Additional gas mixing head O (chamber with O...

  • Page 20: Control Panel On The Rear Of The Chamber

    Control panel on the rear of the chamber (10) Figure 7: Rear control panel CB with O control and options Miniature fuse External socket for extra-low voltage supply (option for CB 160 / CB 220, chap. 15.6) DIN socket for analog outputs 4-20 mA (option, chap. 15.5) Quick acting closure socket for CO Quick acting closure socket for O (chamber with O...

  • Page 21: Completeness Of Delivery, Transportation, Storage, And Installation

    Note on second-hand chambers (Ex-Demo-Units): Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly. Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before commissioning the chamber.

  • Page 22: Storage

    The chambers can be stacked on top of each other (two chambers maximum). For safe stacking that is easy to maintain, use the original BINDER stacking stand (chap. 15.8.1) or the stacking adapter (chap. 15.8.2).
  • Page 23 In order to avoid contamination, never place the chamber directly on the floor. CAUTION Danger of overheating. Damage to the chamber. ∅ Do NOT set up chambers in non-ventilated recesses.  Ensure sufficient ventilation for dispersal of the heat. Do not install or operate the chamber in potentially explosive areas. DANGER Explosion hazard.
  • Page 24 Notes on handling carbon dioxide (CO Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation or a suitable connection to an exhaust system. We recommend installing a CO warning system.
  • Page 25 Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible. General information for safe handling of oxygen: • Make sure training of personnel on hazards of oxygen enrichment and necessary safety measures.

  • Page 26: Installation And Connections

    30 kg / 66 lb Permadry™ water pan The Permadry™ system developed by BINDER is an effective and easy to handle system that ensures high humidity inside the chamber without any condensation forming on the inner surfaces. The Permadry™ water pan consists of two pans in which the outer one is heated and the inner one cooled.

  • Page 27: Connecting The O

    • We recommend cleaning and refilling the pans 2 to 3 times a week. For evacuation, remove the Permadry™ water pan. • We recommend using distilled, sterile water to achieve optimum growth results. Any corrosive damage that may arise following the use of water of different quality or by additives is excluded from the liability agreement.

  • Page 28: Gas Connections

    Gas connections General information for safe handling of gas cylinders: • Store and use gas cylinders only in well ventilated areas. • Open the gas cylinder valve slowly to avoid pressure surges. • Secure gas cylinders during storage and use against falling (chaining). •...

  • Page 29: Connection Of The Co Gas Cylinder

    4.4.1 Connection of the CO gas cylinder Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation or a suitable connection to an exhaust system.

  • Page 30: Connection Of The O Gas Cylinder (Chamber With O Control And Optional Alternative Control Range 10 Up To 95 Vol.-% O )

    The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 22.4) and refer to a connection pressure of 2.0 bar / 29 psi. Decreasing supply pressure will result in longer recovery times. Conversion table for gas inlet pressures, bar –...

  • Page 31: Connection Of The N

    Establishing the connection to the chamber Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp. Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (5) DN 6 on the chamber rear, as described in chap.

  • Page 32: Connecting The Gas Hose To The Chamber Rear (For Co )

    Only use the supplied hose nozzle to connect to the quick acting closure socket. Otherwise, the quick acting closure socket may leak, and/or it may become impossible to connect the original hose nozzle. In this case, please contact BINDER Service. CB (E6.1) 07/2017...

  • Page 33: Gas Cylinder Connection Kits (Option)

    Remove the rubber cover (b) by pulling it off. Chamber rear Figure 13: Connecting the hose lead to the gas cylinder Now fit the hose nozzle (c) in the quick acting closure socket. To remove the connection, pull the hose nozzle off the quick acting closure socket.

  • Page 34: Electrical Connection

    9x40-0088 CB 60 Shock-proof plug 200-240 V (1N~) 50/60 Hz 10 A 9x40-0090 9x40-0089 CB 60-UL NEMA 5-15P 100-120 V (1N~) 50/60 Hz 16 A 9x40-0091 9x40-0092 9x40-0100 CB 160 Shock-proof plug 200-240 V (1N~)

  • Page 35: Handling And Aligning The Divided Inner Door, Gas Proof (Optional Equipment)

    To completely separate the chamber from the power supply, you must disconnect the power plug. Install the chamber in a way that the power plug is easily accessible and can be easily pulled in case of danger. Handling and aligning the divided inner door, gas proof (optional equipment) Rubber profile gasket of individual glass door of...

  • Page 36: Start Up

    To reduce odors quickly we recommend heating up the chamber to its nominal temperature for one day and in a well-ventilated location. WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.

  • Page 37: Functional Overview Of The T4.12 Chamber Controller

    Programming can be done directly through the keypad of the controller or graphically through the software APT-COM™ 3 DataControlSystem (option, chap. 15.1) specially developed by BINDER. Temperature values values...

  • Page 38: Menu Structure

    Menu structure Fixed value 08.03.2013 05:05:06 Setpoint Actual value 37.0 37.2 Temperature [°C] [Vol.-%] Initial view (sample values, chamber with O control). 20.7 20.7 [Vol.-%] Press the desired menu button. User Quick menu Menu From the Initial view you have access to different menus using the menu buttons “User”, “Quick menu”, or “Menu”.

  • Page 39: Quick Menu

    Fixed value 08.03.2013 05:05:06 ..\ Menu Settings Measurement chart Interior socket (optional) Sensor adjustment General menu (next page) Service contact System information Close Home Switching between the operating modes “control off” or “fixed value”, chap. Controller mode 6.2.1 Event list Display of status information and errors, chap.

  • Page 40: User Menu

    Measurement chart Graphical display of the measured values, chap. 13 Active alarms Alarm settings, chap. 11.5 Temperature setpoint Temperature setpoint entry, chap. 7.3 CO2 setpoint setpoint entry, chap. 7.3 O2 setpoint setpoint, chap. 7.3 Safety controller setpoint Setting the safety controller setpoint, chap. 14.2.2 Hot-air sterilization Performing a hot-air sterilization at 180 °C / 356 °F, chap.

  • Page 41: Operating Modes

    Operating modes In the “control off” mode (chap. 6.2.1), the controller is non-functional and displays only the actual values. There is no heating or refrigeration and no inlet of CO or O (chamber with O control). The temperature approximates the ambient value. You can enter the desired setpoint values in “fixed value”...
  • Page 42 Control off 08.03.2013 05:05:06 ..\ Menu Controller mode Event list Alarms General menu. Setpoints The controller mode “Fixed value” or “Control off” is Safety controller indicated in the display headline. Import/Export Hot-air sterilization Humidity control Close Home Go back to the initial view with “Home”. Control off 08.03.2013 05:05:06 Setpoint...

  • Page 43: Deactivating The O

    Deactivating the O control and O pressure alarms (chamber with O control) 6.3.1 Required gas supply of the chamber with O control In the chamber with O control, cell growth can be additionally influenced by a variable oxygen content. Depending on the required range of O2 concentration, the type of gas which is required may vary: •...

  • Page 44: Activating / Deactivating The O Control And O Pressure Alarms

    6.3.2 Activating / deactivating the O control and O pressure alarms To activate or deactivate the O control and O pressure alarms, go to Menu > Controller mode > O2 control Fixed value 08.03.2013 05:05:06 Submenu “O2 control”. ..\ O2 control The current settings are displayed.

  • Page 45: Humidity Control Of The Permadry™ System

    If the ambient temperature deviates by more than +/- 5 °C from the values recommended by the manufacturer, the conditions for maximum air humidity with condensation-free inner chamber are no longer guaranteed. Contact BINDER Service for assistance. CB (E6.1) 07/2017...

  • Page 46: Turning On / Off The Interior Socket Voltage (With Optional Interior Socket)

    Turning on / off the interior socket voltage (with optional interior socket) For chambers equipped with the optional interior socket (chap. 15.4) you can turn on and off the voltage of the interior socket. To turn on or off the interior socket voltage, go to Menu >...

  • Page 47: Information

    Submenu “System information” (sample values). Parameter version: 511B-0004-0000 Firmware version (1): 521C-0001-002E Firmware version (2): 521B-0005-0023 Close Home To display the BINDER Service contact data, go to Menu > Service contact Fixed value 08.03.2013 05:05:06 ..\ Service contact Service hotline Submenu “Service contact”.

  • Page 48: Setpoint Entry

    Setpoint entry Setting ranges Temperature 7 °C / 12.6 °F above ambient up to 60 °C / 140 °F 0 vol.-% up to 20 vol.- % (chamber with O control) 0.2 vol.-% up to 95 vol.-% When changing the temperature setpoint, check the setting of the overtemperature safety controller class 3.1 (chap.
  • Page 49 WARNING High concentration of O (> 21 % O Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries. ∅ Do NOT set up chambers in non-ventilated recesses  Ensure technical ventilation measures ...

  • Page 50: Entering The Setpoints Via "Quick Menu

    Entering the setpoints via “quick menu” To enter setpoints via quick menu, go to Quick menu. Fixed value 08.03.2013 05:05:06 ..\ Quick menu Measurement chart Active alarms “Quick menu”. Temperature setpoint (item “O2 setpoint” only coming with CO2 setpoint chamber with O control) O2 setpoint Select the desired parameter...
  • Page 51 Setting the CO concentration To enter the CO setpoint, go to Quick menu > CO2 setpoint Fixed value 08.03.2013 05:05:06 ..\ CO2 setpoint Entry menu “CO2 setpoint”. [Vol.-%] Select each number with the operating button and press the operating button to confirm. Setting range: 0 vol.-% up to 20 vol.-%.

  • Page 52: Entering The Setpoints Via General Menu

    Entering the setpoints via general menu To enter setpoints via general menu, go to Menu > Setpoints Fixed value 08.03.2013 05:05:06 ..\ Setpoints Temperature setpoint CO2 setpoint Submenu “Setpoints”. O2 setpoint (item “O2 setpoint” only coming with chamber with control) Select the desired parameter and press the operating button.
  • Page 53 Setting the CO concentration To enter the CO setpoint, go to Menu > Setpoints > CO2 setpoint Fixed value 08.03.2013 05:05:06 ..\ CO2 setpoint Entry menu “CO2 setpoint”. [Vol.-%] Select each number with the operating button and press the operating button to confirm. Setting range: 0 vol.-% up to 20 vol.-%.

  • Page 54: Key Lock

    Key lock The key lock function serves to block the access to the controller. When the “key lock” function is activated, the controller changes to the initial view and can only be changed when entering the current password. To configure the key lock function, go to User >...

  • Page 55: Directly Activating The Key Lock

    Directly activating the key lock To directly activate the key lock, go to User > Key lock > Key lock On Fixed value 08.03.2013 05:05:06 ..\ Key lock Key lock On Automatic key lock Password Submenu “Key lock”. Select “Key lock On” to activate the key lock function immediately and press the operating button.
  • Page 56 Under “Waiting time [min]” you can enter the waiting time, after which the key lock will be automatically activated. This time starts running off after the last entry to the controller. To enter it, go to User > Key lock > Automatic key lock > Waiting time [min] Enter wait time [min] 08.03.2013 05:05:06 ..\ User\Waiting time...

  • Page 57: Changing The Password For Unlocking The Key Lock

    This symbol on the controller displays indicates that the “key lock” function is activated. Changing the password for unlocking the key lock To change the password for unlocking the key lock, go to User > Key lock > Password Fixed value 08.03.2013 05:05:06 ..\ Key lock Key lock On...

  • Page 58: General Controller Settings

    General controller settings In the "Settings" submenu, you can enter the date and time, select the language for the menus and the desired temperature unit, perform the configuration for the controller’s communication functions, and reset the controller to factory settings. To access the "Settings"...

  • Page 59: Setup Wizard

    Setup wizard The setup wizard will guide you sequentially through the important menus to configure your chamber • Menu language • Device name • Date and time • IP address • Subnet mask • Network name • Gateway • DNS 1 •...
  • Page 60 Function “Set date” Fixed value 08.03.2013 05:05:06 ..\ Select date (DD.MM.YYYY) 8.03.2013 Entry menu “Select date”. The current date is shown. If it is incorrect, enter the correct date with the operating button. Press the “Ok” button to confirm. Ins Pos1 End Ok 1 2 3 4 5 6 7 8 9 Close Home...

  • Page 61: Selecting The Menu Language Of The T4.12 Controller

    Selecting the menu language of the T4.12 controller The T4.12 chamber controller communicates via a comprehensible menu navigation in plain text in a selectable language. To select the desired menu language, go to Menu > Settings > Sprache, Language, Langue, Idioma, Lingua Fixed value 08.03.2013 05:05:06 ..\ Sprache, Language, Langue, Idioma, Lingua...

  • Page 62: Changing The Temperature Unit

    Changing the temperature unit To select the temperature unit, go to Menu > Settings > Temperature unit Fixed value 08.03.2013 05:05:06 ..\ Temperature unit Celsius [°C] Fahrenheit [°F] Kelvin [K] Submenu “Temperature unit”. Select the desired temperature unit” and press the operating button. Close Home Go back to the “Settings”...

  • Page 63: Factory Reset

    The settings of this submenu are required for networking chambers with an Ethernet interface, e.g. to connect them with BINDER’s communication software APT-COM™ 3 DataControlSystem. You can display the chamber‘s IP address that has been assigned by your DHCP server or manually assign the IP address.
  • Page 64 Fixed value 08.03.2013 05:05:06 ..\ Network settings DNS 1 DNS 2 Submenu “Network settings” (next page). Close Home Show network settings Overview of the entire network configuration DHCP On/Off Switching on and off the DHCP state MAC address Displaying the MAC address IP address Entering the desired IP address Subnet mask...
  • Page 65 Display the MAC address To identify the chamber in the Ethernet network you can display the chamber’s MAC address. Fixed value 08.03.2013 05:05:06 ..\ MAC address 00-04-A3-B1-00-B9 Submenu “MAC address” (example value) The MAC address is displayed. Close Home Go back to the “Network settings” menu with “Close” or to the initial view with “Home”. Enter the IP address: Fixed value 08.03.2013 05:05:06...
  • Page 66 Enter the chamber name: Fixed value 08.03.2013 05:05:06 ..\ Chamber name B_E6.1 Entry menu “Chamber name”. Enter the desired chamber name with the operating button. Press the “Ok” button to confirm. End Ok _ : A B D E F G H I J K Close Home Go back to the “Network settings”...

  • Page 67: Display Of The Network Configuration

    Enter the DNS 1 or DNS 2: Fixed value 08.03.2013 05:05:06 ..\ DNS 1 (n.n.n.n.) 92.168.0.1 Entry menu “DNS 1” or “DNS 2” (example value) Enter the desired number with the operating button. Press the “Ok” button to confirm. Ins Pos1 End Ok 0 2 3 4 5 6 7 8 9 , Close Home...

  • Page 68: Rs 422 Address (With Optional Rs 422 Interface)

    For chambers equipped with the optional RS 422 interface, the RS 422 address serves to identify the chamber in a network and to establish communication with the optional BINDER communication software APT-COM™ 3 DataControlSystem. The factory default setting is “1”.

  • Page 69: Data Transfer Via Usb Interface

    Data transfer via USB interface The USB port is located in the instrument box. To access the submenus for data transfer, go to Menu > Import/Export Fixed value 08.03.2013 05:05:06 ..\ Import/Export to USB drive Export to USB drive Import from USB drive Submenu “Import/Export to USB drive”.

  • Page 70: Importing Data From Usb Drive

    If no USB device has been connected, the message “No USB device found” is displayed. Confirm the message with “OK” and insert the USB medium. This symbol on the controller display in the initial view indicates that data are being transmitted via the USB port.

  • Page 71: Notifications And Alarms

    Notifications and Alarms 11.1 Notifications overview Icon Signification Icon Signification control deactivated. No O Fixed value operation concentration alarms will occur. (chamber with O control) Heating active pressure alarm deactivated pressure alarm deactivated Key lock activated (chamber with O control) pressure alarm deactivated Copying data via USB (chamber with O...
  • Page 72 Contact BINDER Service. defective Inform service - sensor adjustment required. Contact BINDER Service. adjust CO2 sensor You can activate / deactivate the buzzer in the “Alarms” submenu (chap. 11.5.3). With an activated buzzer there is an audible alert with an alarm. You can reset it in the “Alarms”...

  • Page 73: Alarm Status

    11.3 Alarm status An alarm message can appear in 3 different states: “Set” • Active alarm. • The corresponding alarm icon is displayed in the initial view The buzzer sounds (if activated). • The “Info” button in the initial view leads to the “Alarms” submenu for alarm acknowledgement. •...

  • Page 74: Confirming An Active "Set" Alarm

    11.4 Confirming an active “set” alarm Fixed value 08.03.2013 05:05:06 Setpoint Actual value 37.0 39.2 Temperature [°C] Initial view with overtemperature safety controller [Vol.-%] alarm. The buzzer sounds (if not deactivated previously). 25.0 25.0 [Vol.-%] Press the “Info” button. User Info Menu Alarm acknowledgement...

  • Page 75: Alarm Configuration And Overview

    11.5 Alarm configuration and overview To access the alarm lists and configuration menu, go to Menu > Alarms Fixed value 08.03.2013 05:05:06 ..\ Alarm Active alarms History Buzzer test Submenu “Alarm”. Buzzer On/Off Select the desired function Alarms On/Off and press the operating button. Door alarm delay Tolerance ranges Close...

  • Page 76: History - List Of All Alarms

    11.5.2 History – list of all alarms To access the overview list of all alarms, go to Menu > Alarms > History This list indicates the moment when an alarm was set and when cleared. Fixed value 08.03.2013 15:05:06 ..\ History 08.03.2013 : Messages of indicated day 14:39:48 Alarm set Safety controller overtemperature Submenu “History”...

  • Page 77: Activating, Deactivating, And Testing The Alarm Buzzer

    11.5.3 Activating, deactivating, and testing the alarm buzzer Alarm buzzer test To access the alarm buzzer functional test, go to Menu > Alarms > Buzzer test Fixed value 08.03.2013 05:05:06 ..\ Buzzer test Submenu “Buzzer test”. Buzzer test: Off The current setting is displayed. Press the operating button to turn on or off the buzzer for test purpose.

  • Page 78: Setting The Delay Time After Opening The Door

    11.5.5 Setting the delay time after opening the door To enter the delay time for the door open alarm, go to Menu > Alarms > Door alarm delay Enter door alarm delay [min] 08.03.2013 05:05:06 ..\ Door alarm delay Entry menu “Door alarm delay” Enter the desired delay time in minutes with the operating button.
  • Page 79 Temperature First you can enter the desired value of the temperature tolerance range: Enter tolerance range 08.03.2013 05:05:06 ..\ Tolerance ranges \ Temperature Entry menu “Temperature”. [°C] Enter the desired value of the temperature tolerance range with the operating button. Setting range: 0 up to infinite Press the “Ok”...
  • Page 80 Then you can enter the delay time for the CO tolerance range alarm: Enter alarm delay [min] 08.03.2013 05:05:06 ..\ CO2 alarm delay Entry menu “CO2 alarm delay” Enter the desired delay time in minutes with the operating button. Setting range: 0 up to infinite Press the “Ok”...
  • Page 81 When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value. Go back to the initial view with “Home”. Service settings Fixed value 08.03.2013 05:05:06 ..\ Tolerance ranges\ Service settings Temperature alarm delay 2...

  • Page 82: Zero-Voltage Relay Alarm Output

    11.6 Zero-voltage relay alarm output The chamber is equipped with a zero-voltage relay output which permits the transmission of some alarm messages to a central monitoring system. The connection is realized as a DIN socket (9) on the chamber rear. A suitable DIN plug is enclosed. Figure 17: Pin configuration of the DIN socket (9) on the chamber rear Pin 1: Pole, Pin 2: Break relay, Pin 3: Make contact ALARM...

  • Page 83: Event List

    Event list The “Event list” displays status information and errors of the current day. You can also access the events of past days. To access the event list, go to Menu > Event list User > Show event list Fixed value 08.03.2013 15:05:18 ..\ Event list 08.03.2013...

  • Page 84: Graphical Display Of The Measured Values

    Graphical display of the measured values To access the graphical display, go to Menu > Measurement chart Fixed value 08.03.2013 05:05:06 0:11 0:16 0:22 Measurement chart view (sample view). Press the “Settings” button. Close Settings Home Fixed value 08.03.2013 05:05:06 Settings Sampling rate Display range...

  • Page 85: Defining The Display Range

    13.2 Defining the display range To define the display range, go to Menu > Measurement chart > Settings > Display range Fixed value 08.03.2013 05:05:06 Display range Temperature Submenu “Display range” (unit with O control). Select the desired parameter and press the operating button. Close Home Fixed value...

  • Page 86: Selecting The Parameters

    13.3 Selecting the parameters Here you can select the parameters, which shall be displayed graphically. To select the parameters, go to Menu > Measurement chart > Settings > Parameters Fixed value 08.03.2013 05:05:06 Parameters Temperature On CO2 On O2 On Submenu “Parameters”...

  • Page 87: Temperature Safety Devices

    The user cannot restart the device again. The protective cut-off device is located internally. Only a service specialist can replace it. Therefore, please contact an authorized service provider or BINDER Service. 14.2 Overtemperature safety controller (temperature safety device class 3.1) The chamber is regularly equipped with an electronic overtemperature safety controller (temperature safety device class 3.1 according to DIN 12880).

  • Page 88: Setting The Safety Controller

    14.2.2 Setting the safety controller To display and to change the current safety controller settings in the “safety controller” submenu, go to Menu > Safety controller Safety controller mode: selection between Limit (absolute) and Offset (relative) Fixed value 08.03.2013 05:05:06 ..\ Safety controller Mode Setpoint...
  • Page 89 Fixed value 08.03.2013 05:05:06 ..\ Safety controller overtemperature Entry menu “Safety controller overtemperature”. [°C] Enter the desired value with the operating button and press the operating button to confirm. Press the “Ok” button to confirm. End Ok 0 1 2 4 5 6 7 8 9 Del Close Home...
  • Page 90 Fixed value 08.03.2013 05:05:06 Setpoint Actual value Chamber temperature [°C] 37.0 37.6 Safety controller mode Offset (relative) Safety controller [°C] 37.6 Overview display with “Offset” mode (example values) Close Home Go back to the initial view with “Home”. CB (E6.1) 07/2017 Page 90/145...

  • Page 91: Options

    BINDER communication software APT-COM™ 3. 15.2 RS 422 interface (option) With this option, the chamber is equipped with a serial interface RS 422 (5), that can connect the BINDER communication software APT-COM™ 3 DataControlSystem. The MAC Address is indicated below the Ethernet interface.

  • Page 92: Interior Socket 230V (Option)

    When operating a CB with silicon access ports, both silicon plugs must tightly close the access ports. If the plugs are inserted in a not-gastight manner, or if plugs are missing, CO gas (chamber with control) may escape into the environment. The CO control and the O control (chamber with O control) only turn off when the chamber door is opened.
  • Page 93 Heat emission of electrical devices connected inside the CO incubator may modify the temperature range. The maximum load must not exceed 3 Amp. WARNING Overload of contacts. Damage to contacts and connection socket. Electrical hazard. Danger of death. ∅ Do NOT exceed the maximum load of 3 A. ∅...

  • Page 94: Analog Outputs For Temperature And Co (Option)

    15.5 Analog outputs for temperature and CO (option) With this option, the chamber is equipped with analog outputs 4-20 mA for temperature and CO . These outputs allow transmitting data to external data registration systems or devices. The connection is realized as a DIN socket (3) on the chamber rear. A suitable DIN plug is enclosed. ANALOG OUTPUT 4-20 mA DC PIN 1: Temperature + PIN 2: Temperature -...

  • Page 95: Binder Gas Supply Service - External Bottle Changer For Co

     When operating electrical devices inside the incubator: Ambient temperature ≤ 25 °C / 77°F.  Before introducing any cells, check the correct ambient temperature. 15.7 BINDER Gas Supply Service – External bottle changer for CO or O (option) The external bottle changer permits automatic switching to a second gas cylinder as soon as the first cylinder is empty.

  • Page 96: Stands

    We recommend not stacking CB incubators directly on top of one another in order to avoid transmission of shocks and vibrations from one chamber to the other. This could happen e.g. while opening or closing the door, cleaning, loading and unloading the chamber. BINDER offers a stacking adapter for direct thermal decoupled stacking of two chamber.

  • Page 97: Reference Measurements

    NaHCO buffered media. These test systems are not suitable for calibrating the BINDER sensor system. Recommended procedure: • Incubate an empty sample with medium for 1/2 day under the same conditions as the cells. You can perform the incubation in a cell culture cylinder or in a 50 ml Falcon tube with open lid.

  • Page 98: Directly Via Chemical Indicator Tubes

    All the necessary equipment must be supplied by one manufacturer only and in a defined test system. Note: These test systems are not very accurate. A typical accuracy is around 10 % of the full-scale value. These test systems are not suitable for calibrating the BINDER sensor system. Figure 26: Example of chemical indicator...

  • Page 99: Temperature Reference Measurement

    16.1.3 Measuring CO directly with an electronic infrared measuring device Another possible way of measuring the CO concentration is by electronic sensor systems. Portable measuring devices are suitable for measuring temperature and CO concentration and can be used both for reference measurements in certified laboratories, and for service purposes. 16.2 Temperature reference measurement When performing a temperature reference measurement using an electronic measuring, and temperature display device, it is important to use a device traceable to an acknowledged standards/calibration...

  • Page 100: Working And Behavior In The Lab

    • Reduce the number of personnel and their movements in the lab by careful positioning all relevant equipment. For practical reasons, install the chamber close to the laminar air flow bench. • Regular microbiological monitoring of the cell culture laboratory. 17.3 Working and behavior in the lab Sources of contamination are often the laboratory personnel themselves (surface germs, oral flora droplet transfer) and handling the equipment and cultures.

  • Page 101: Handling The Co Incubator

    Condensation in the inner chamber represents a particular risk of contamination. The Permadry™ system developed by BINDER is an effective and easy way to ensure high humidity (≥95 % r.h.) inside the chamber without any condensation forming on the inner surfaces.
  • Page 102 • Regularly use the hot air sterilization function (chap. 18.3) following cleaning. The shelves and the emptied water pan can remain inside the chamber during this operation. • Have replaced the CO sterile filter (gas fine filter) once or twice a year. What to do in case of contamination? •...

  • Page 103: Cleaning, Decontamination / Disinfection, And Sterilization

    We recommend using the neutral cleaning agent Art. No. 1002-0016 for thorough cleaning. Any corrosive damage that may arise following use of other cleaning agents is excluded from liability by BINDER GmbH. Any corrosive damage caused by a lack of cleaning, is excluded from liability by BINDER GmbH. CAUTION Danger of corrosion.
  • Page 104 ∅ Do NOT spray cleaning detergents directly on the CO sensor.  Wipe the sensor with a lint-free cloth soaked with cleaning agent. Use only the detergents or disinfectants recommended by BINDER. Other products are not approved, since they could in particular damage the sensor.

  • Page 105: Decontamination / Chemical Disinfection Of The Chamber

    ∅ Do NOT spray the disinfectant directly on the CO sensor.  Wipe the sensor with a lint-free cloth soaked with the disinfectant. Use only the detergents or disinfectants recommended by BINDER. Other products are not approved, since they could in particular damage the sensor.

  • Page 106: Hot-Air Sterilization At 180 °C / 356 °F

    Recommended precautions: To protect the eyes use sealed protective goggles. CAUTION Eye contact. Eye damage due to chemical burns. ∅ Do NOT empty into drains.  Wear protective goggles. Following frequent use of the disinfectant spray and prior to hot-air sterilization, remove any agent remainder by using the neutral cleaning agent and then a moistened towel to avoid formation of permanent residues.

  • Page 107: Performing A Hot-Air Sterilization

    Note: in the case when two chambers are directly stacked on top of each other without using the original BINDER stacking stand or the BINDER stacking adapter, the exact maintenance of the incubation set parameters in one of the chambers while carrying out sterilization in the other one cannot be assured.
  • Page 108 The message “Warning: Samples and water must be removed!” is displayed. Confirm the message with “OK” and make sure that all samples were removed from the CB before starting the hot-air sterilization. Fixed value 08.02.2017 05:05:06 ..\ Start hot-air sterilization Do not start Really start Submenu “Start hot-air sterilization”.

  • Page 109: Aborting The Hot-Air Sterilization - General Procedure

    If a hot-air sterilization is already running and you try to start it again, the message „Hot air sterilization running“ is displayed. Confirm the message with “OK”. Opening the outer door during sterilization leads to aborting the automatic sterilization cycle. CAUTION Interruption of the temperature reaction time.

  • Page 110: Aborting Hot-Air Sterilization After Less Than 4 Hours

    Aborting the sterilization cycle means cancelling it before the entire 10-hour duration is completed. Whether effective sterilization has occurred depends on the time that has elapsed bevor abortion. • Aborting sterilization after less than 4 hours: Prevents effective sterilization. This symbol on the controller display indicates that the hot-air sterilization was aborted. The interior may still be hot.
  • Page 111 Fixed value 08.02.2017 05:05:06 Setpoint Actual value 37.0 60.2 Temperature [°C] [Vol.-%] Initial view after aborting the hot-air sterilization (sample values). 20.0 For further information, press the “Info” button. [Vol.-%] User Menu Info Sterilization information 08.02.2017 05:05:06 Sterilization information STERILIZATION ABORTED Information menu “Sterilization information”.

  • Page 112: Terminating Successful Sterilization During The Cooling-Down Phase (Abortion After More Than 4 Hours)

    18.3.5 Terminating successful sterilization during the cooling-down phase (abortion after more than 4 hours) The duration of the entire sterilization is approx. 10 hours. If you want to shorten the sterilization procedure in order to save time, you can abort it during the cooling-down phase, i.e. no sooner than after 4 hours.

  • Page 113: Terminating The Completed Sterilization Cycle

    To abort the sterilization cycle proceed as described in chap. 18.3.3. The symbol then disappears from the controller display. The inner chamber and parts inside can still be hot. Do not touch. The defined cooling down phase lasts 6 hours until reaching 37 °C / 98.6°F and is followed by a holding phase at 37 °C / 98.6°F of unlimited duration If you prefer accelerating the cooling- down phase, proceed as follows: •...
  • Page 114 Fixed value 08.02.2017 05:05:06 Setpoint Actual value 37.0 37.2 Temperature [°C] [Vol.-%] Initial view after finishing the hot-air sterilization cycle (sample values). 20.0 For further information, press the “Info” button. [Vol.-%] User Menu Info Sterilization information 08.02.2017 05:05:06 Sterilization information STERILIZATION FINISHED Information menu “Sterilization information”.

  • Page 115: Maintenance And Service

    With an increased amount of dust in the ambient air, clean the Peltier fan grid (7) by suction or blowing several times a year. We recommend taking out a maintenance agreement. Please consult BINDER Service. BINDER telephone hotline: +49 (0) 7462 2005 555...

  • Page 116: Checking The Air Jacket Heating Fan

    19.5 Sending the chamber back to BINDER GmbH If you return a BINDER product to us for repair or any other reason, we will only accept the product upon presentation of an authorization number (RMA number) that has previously been issued to you. An authorization number will be issued after receiving your complaint either in writing or by telephone prior to your sending the BINDER product back to us.

  • Page 117: Disposal

    Disposal 20.1 Disposal of the transport packing 20.1.1 Outer chamber packing Packing element Material Disposal Straps to fix packing on pallet Plastic Plastic recycling (no image) Shipping box Cardboard Paper recycling Edge stuffing, top PE foam Plastic recycling Removal Cardboard Paper recycling Plastic Plastic recycling...

  • Page 118: Decommissioning

    (Elektro- und Elektronikgerätegesetz, ElektroG from 20 October 2015, BGBl. I p. 1739) or contact BINDER service who will organize taking back and disposal of the chamber according to the German national law for electrical and electronic equipment (Elektro- und Elektronikgerätegesetz, ElektroG from 20 October 2015, BGBl.

  • Page 119: Disposal Of The Chamber In The Member States Of The Eu Except For The Federal Republic Of Germany

    According to Annex I of Directive 2012/19/EU of the European Parliament and of the Council on waste electrical and electronic equipment (WEEE), BINDER devices are classified as “monitoring and control instruments” (category 9) only intended for professional use“. They must not be disposed of at public collecting points.

  • Page 120: Disposal Of The Chamber In Non-Member States Of The Eu

    Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling according to Directive 2012/19/EU. The devices must be free from toxic, infectious or radioactive substances in order to eliminate any health hazards to the employees of the recycling companies.

  • Page 121: Troubleshooting

    Troubleshooting Only qualified service personnel authorized by BINDER must perform repair. Repaired chambers must comply with the BINDER quality standards. 21.1 General Fault description / indication Possible cause Required measures No power supply. Check connection to power supply. Check power supply for voltage of Wrong voltage.
  • Page 122 Temperature control not Calibrate and adjust the temperature The displayed actual adjusted. control. temperature value deviates Transfer the cultures to another largely compared with a Temperature sensor defective. incubator and contact BINDER reference method.. Service. CB (E6.1) 07/2017 Page 122/145...

  • Page 123: Gas Cylinder Pressure Too Low

    Alarm message obstruction, clean or replace it. “Low pressure CO ” Confirm the alarm (chap. 11.4). If Controller malfunction. necessary, contact BINDER Service. Pressure sensor system Confirm the alarm (chap. 11.4). defective. Contact BINDER Service. cylinder is not connected Confirm the alarm (chap. 11.4).

  • Page 124: Gas Concentration

    Alarm message Confirm the alarm (chap. 11.4). If “Low pressure N ” Controller malfunction necessary, contact BINDER Service. Pressure sensor system Confirm the alarm (chap. 11.4). defective. Contact BINDER Service. 21.4 Gas concentration Fault description / indication...
  • Page 125 The Transfer the cultures to another pH indicator of the cell culture sensor defective. incubator and contact BINDER medium changes its normal Service. color. control not adjusted. Calibrate and adjust O control.

  • Page 126: Sterilization

    Replace door gaskets chamber. Doors not closed properly. Close door properly. Humidity set too high. Reduce humidity (chap. 6.4) Place the chamber on a BINDER Chamber placed on very cold stand to increase the distance to the floor. floor. Door gaskets defective.

  • Page 127: Controller

    Fault description / indication Possible cause Required measures Place the chamber on a BINDER Chamber placed on very cold stand to increase the distance to the floor. floor. Door gaskets defective. Replace door gaskets Condensation on the divided inner door, gas-proof.

  • Page 128: Technical Description

    (certified since December 1996 by TÜV CERT). All test equipment used is subject to the administration of measurement and test equipment that is also a constituent part of the BINDER QM DIN EN ISO 9001 systems. They are controlled and calibrated to a DKD-Standard at regular intervals.

  • Page 129: Cb Technical Data

    Do NOT place samples outside this usable volume. Do NOT load this volume by more than half to enable sufficient airflow inside the chamber. Do NOT divide the usable volume into separate parts with large area samples. Do NOT place samples too close to each other in order to permit circulation between them and thus obtain a homogenous distribution of temperature, CO and O (chamber with O...
  • Page 130 All technical data is specified for unloaded chambers with standard equipment at an ambient temperature of 22 +/- 3 °C / 71.6 +/- 5.4 °F and a power supply voltage fluctuation of +/-10%. Technical data is determined in accordance to BINDER Factory Standard Part 2:2015 and DIN 12880:2007. CB (E6.1) 07/2017...

  • Page 131: Equipment And Options

    22.5 Equipment and Options To operate the chamber, use only original BINDER accessories or accessories / components from third-party suppliers authorized by BINDER. The user is responsible for any risk arising from using unauthorized accessories. Regular equipment Multifunction controller T4.12 for temperature and CO...

  • Page 132: Accessories And Spare Parts (Extract)

    Spatial temperature measurement acc. to DIN 12880 including certificate 22.6 Accessories and spare parts (extract) BINDER GmbH is responsible for the safety features of the chamber only, provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.

  • Page 133: Important Conversion Data For Non-Si Units

    8012-1550 Spatial temperature measurement including certificate (27 measuring points) 8012-1592 For information on components not listed here, please contact BINDER Service. 22.7 Important conversion data for non-SI units 1 ft = 0.305 m 1 m = 100 cm = 3.28 ft = 39.37 inch 1 mbar = 0.0145 psi...

  • Page 134: Dimensions Cb 60

    22.9 Dimensions CB 60 (Dimensions in mm) CB (E6.1) 07/2017 Page 134/145...

  • Page 135: Dimensions Cb 160

    22.10 Dimensions CB 160 (Dimensions in mm) CB (E6.1) 07/2017 Page 135/145...

  • Page 136: Dimensions Cb 220

    22.11 Dimensions CB 220 (Dimensions in mm) CB (E6.1) 07/2017 Page 136/145...

  • Page 137: Certificates And Declarations Of Conformity

    Certificates and declarations of conformity 23.1 EU Declaration of conformity CB (E6.1) 07/2017 Page 137/145...
  • Page 138 CB (E6.1) 07/2017 Page 138/145...

  • Page 139: Product Registration

    Product registration CB (E6.1) 07/2017 Page 139/145...

  • Page 140: Contamination Clearance Certificate

    Contamination clearance certificate 25.1 For chambers located outside the USA and Canada Declaration regarding safety and health Erklärung zur Sicherheit and gesundheitlichen Unbedenklichkeit The German Ordinance on Hazardous Substances (GefStofV), and the regulations regarding safety at the workplace, require that this form be filled out for all products that are returned to us, so that the safety and the health of our employees can be guaranteed.
  • Page 141 Kind of transport / transporter / Transportweg/Spediteur: Transport by (means and name of transport company, etc.) Versendung durch (Name Spediteur o.ä.) ___________________________________________________________________________________ Date of dispatch to BINDER GmbH / Tag der Absendung an BINDER GmbH ___________________________________________________________________________________ CB (E6.1) 07/2017 Page 141/145...
  • Page 142 We are aware that, in accordance with Article 823 of the German Civil Code (BGB), we are directly liable with regard to third parties, in this instance especially the employees of BINDER GmbH, who have been entrusted with the handling / repair of the chamber / component. / Es ist uns bekannt, dass wir gegenüber...

  • Page 143: For Chambers In The Usa And Canada

    Please complete this form and the Customer Decontamination Declaration (next 2 pages) and attach the required pictures. E-mail to: IDL_SalesOrderProcessing_USA@binder-world.com After we have received and reviewed the complete information we will decide on the issue of a RMA number. Please be aware that size specifications, voltage specifications as well as performance www.binder-world.us...
  • Page 144 Customer (End User) Decontamination Declaration Health and Hazard Safety declaration To protect the health of our employees and the safety at the workplace, we require that this form is completed by the user for all products and parts that are returned to us. (Distributors or Service Organizations cannot sign this form) NO RMA number will be issued without a completed form.
  • Page 145 4.5 Shipping laws and regulations have not been violated. I hereby commit and guarantee that we will indemnify BINDER Inc. for all damages that are a consequence of incomplete or incorrect information provided by us, and that we will indemnify and hold harmless BINDER Inc.

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