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Operating Manual
CB-S / CB-S-UL (E7)
CO
Incubator
2
with FPI-sensor system and RD4 controller
Model
Model version
CB-S 170
CBS170-230V
CB-S 170
CBS170-230V-D
CB-S 170-UL
CBSUL170-120V
CB-S 170-UL
CBS170UL-120V-D
CB-S 260
CBS260-230V
CB-S 260-UL
CBSUL260-120V
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany
Phone: +49 7462 2005 0
Fax: +49 7462 2005 100
Internet: http://www.binder-world.com
Service Hotline: +49 7462 2005 555
Service Fax: +49 7462 2005 93 555
Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3
Service Hotline Asia Pacific: +852 390 705 04 or +852 390 705 03
Issue 01/2025
Equipment
Access port 30 mm, left
Access port 30 mm, left
Art. no.
9640-0001
9640-0029
9640-0002
9640-0030
9640-0003
9640-0004
Art. No. 7001-0402
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Summary of Contents for Binder CB-S Series
- Page 1 Address: Post office box 102, 78502 Tuttlingen, Germany Phone: +49 7462 2005 0 Fax: +49 7462 2005 100 Internet: http://www.binder-world.com Service Hotline: +49 7462 2005 555 Service Fax: +49 7462 2005 93 555 Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3 ...
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Page 2: Table Of Contents
Contents SAFETY ........................6 Personnel Qualification ........................6 Operating manual ..........................6 Legal considerations ........................... 6 1.3.1 Intellectual property ........................7 Structure of the safety instructions in the operating manual ............... 7 1.4.1 Signal word panel ........................7 1.4.2 Safety alert symbol ........................8 1.4.3 Pictograms .......................... - Page 3 TEMPERATURE AND CO SET-POINT ENTRY ..........38 Temperature set-point entry ......................38 set-point entry ..........................38 PLACING SAMPLES IN THE INCUBATOR ............39 SETTING SPECIAL CONTROLLER FUNCTIONS ..........40 Idle mode............................40 Deactivated CO control ........................41 10. PASSWORD ......................41 10.1 Password request ..........................
- Page 4 16. ETHERNET NETWORK SETTINGS ..............62 16.1 Showing the network settings ......................62 16.1.1 Showing the chamber‘s MAC address ..................62 16.1.2 Showing the IP address ......................62 16.1.3 Showing the subnet mask ....................... 63 16.1.4 Showing the standard gateway ....................63 16.1.5 Showing the DNS server address ...................
- Page 5 24.5.4 Humidity ..........................94 24.5.5 Controller ..........................94 24.5.6 Sterilization ..........................95 24.6 Sending the chamber back to BINDER GmbH ................. 95 25. DISPOSAL......................96 25.1 Disposal of the transport packing ...................... 96 25.1.1 Outer chamber packing......................96 25.1.2 Packing inside the chamber, equipment ................. 96 25.2 Decommissioning ..........................
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Page 6: Safety
Operating Manual. This Operating Manual is supplemented and updated as needed. Always use the most recent version of the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-to-date- ness and validity of this Operating Manual. -
Page 7: Intellectual Property
In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual. This operating manual cannot cover all conceivable applications. If you would like additional information, or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or contact us directly, e.g. -
Page 8: Safety Alert Symbol
CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury NOTICE Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product and/or its functions or of a property in its proximity. 1.4.2 Safety alert symbol Use of the safety alert symbol indicates a risk of injury. -
Page 9: Prohibition Signs
The following labels are located on the chamber: Pictograms (Warning signs) Hot surface Risk of injury (UL chambers only). Observe the safety instructions in the operating manual. Information QR-Code and URL to contact the BINDER Support Center CB-S / CB-S-UL (E7) 01/2025 page 9/115... -
Page 10: Type Plate
CB-S-UL Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER Service for these replacements. Type plate Position of type plate: left chamber side (seen from front), at the bottom in the middle. -
Page 11: Ukca Label
Indications of the type plate (example) Indication Information BINDER Manufacturer: BINDER GmbH CB-S 170 Model designation CO2 Incubator Device name: CO Incubator Serial No. 000000000000 Serial No. of the chamber Built 2025 Year of construction 180 °C Nominal temp. Nominal temperature 356 °F... -
Page 12: General Safety Instructions On Installing And Operating The Chamber
DGUV rule 110-007, issued by the employers’ liability insurance association). BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts. -
Page 13: Precautions When Working With Co 2 Gas
DANGER Electrical hazard by water entering the chamber. Deadly electric shock. ∅ The chamber must NOT become wet during operation, cleaning, or maintenance. ∅ Do NOT install the chamber in damp areas or in puddles. Set up the chamber in a splash-proof manner. The chambers were produced in accordance with VDE regulations and were routinely tested in accordance to VDE 0411-1 (IEC 61010-1). -
Page 14: Precautions When Handling Gas Cylinders
1.10 Precautions when handling gas cylinders General information for safe handling of gas cylinders: • Store and use gas cylinders only in well-ventilated locations. • Open the gas cylinder valve slowly to avoid pressure surges • Secure gas cylinders during storage and use against falling (chaining). •... - Page 15 Such ingredients include in particular acids and hal- ides. Any corrosive damage caused by such ingredients is excluded from liability by BINDER GmbH. The chamber does not dispose of any measures of explosion protection.
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Page 16: Foreseeable Misuse
WARNING: If customer should use a BINDER chamber running in non-supervised continu- ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible. -
Page 17: Residual Risks
1.13 Residual Risks The unavoidable design features of a chamber, as well as its proper field of application, can also pose risks, even during correct operation. These residual risks include hazards which, despite the inherently safe de- sign, existing technical protective equipment, safety precautions and supplementary protective measures, cannot be ruled out. -
Page 18: Operating Instructions
• Inappropriate repairs which do not meet the quality standard specified by BINDER • Use of replacement parts other than BINDER original replacement parts • Electrical safety analysis not performed after repairs 1.14 Operating instructions Depending on the application and location of the chamber, it is recommended that the operator of the chamber provides the relevant information for safe operation of the chamber in a set of operating instruc- tions. -
Page 19: Chamber Description
A highly precise, drift-free CO infrared measuring system in combination with the permanent mixture of CO gas through a special proprietary gas mixing head developed by BINDER allows precise and constant CO concentrations for long periods. This creates optimum growth conditions for cultures. -
Page 20: Chamber Overview
The chamber is regularly equipped with an Ethernet interface for computer communication, enabling mon- itoring via a network. The BINDER APT-COM™ 4 Multi Management Software (accessory, chap. 20.4) permits networking of up to 100 chambers and connection to a computer, as well as recording and repre- senting temperature and CO data. -
Page 21: Inner Chamber
Inner chamber (11) (10) Figure 7: CO incubator CB-S / CB-S-UL (example CB-S 170), outer door open Triangle instrument panel with RD4 controller for temperature and CO Glass door handle Water pan Shelves (not used) Pt 100 temperature sensor sensor (10) Gas mixing head for CO (11) -
Page 22: Connection Panel On The Rear Of The Chamber
(12b) Socket for IEC connector plug / power cable 100-120 V AC for CB-S-UL (13) DIN-socket for zero-voltage relay alarm outputs (14) DIN socket for analog outputs 4-20 mA (available by BINDER INDIVUDUAL Customized Solutions) (15) Ethernet interface for computer communication (16a) Miniature fuse T10 A (L) 250 V AC for CB-S... -
Page 23: Chamber Doors
Chamber doors The outer chamber door is equipped with a heater on its inner side. The door must be closed while the chamber is operating normally in order to ensure stable climatic conditions in the inner chamber. An additional glass door enables viewing of the samples without disturbing the temperature in the interior and contaminating the samples sealing the interior of the chamber. -
Page 24: Guidelines For Safe Lifting And Transportation
Note on second-hand chambers (Ex-Demo-Units): Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flaw- lessly. Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before commissioning the chamber. -
Page 25: Location Of Installation And Ambient Conditions
Location of installation and ambient conditions Notes on the location of installation Set up the chamber on a flat, even surface, free from vibration and in a well-ventilated, dry location. The chambers are designed for setting up inside a building (indoor use). Freestanding chambers are suitable for installation on tables or on the optionally available stand. - Page 26 Minimum distances • Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in. To completely separate the chamber from the power supply, you must disconnect the power plug. Install the chamber in a way that the power plug is easily accessible and can be easily pulled in case of danger.
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Page 27: Installation And Connections
Installation and connections Shelves You can put the shelves in different positions at the line of channel beads in the inner chamber. Hold the shelf straight and then insert it so it will go smoothly inside the chamber. Permitted shelf loads: Maximum load on one single shelf: 10 kg / 22 lb Maximum total load on all shelves:... -
Page 28: Water Pan
The CO sensor is temperature resistant up to a maximum temperature of 60 °C / 140 °F. NOTICE Danger of damage to the CO sensor by excess temperature. Damage to the CO sensor. ∅ Do NOT autoclave the CO Sensor. ∅... -
Page 29: Gas Connection
Make sure that the water pan has firm contact with the inner chamber bottom and rests tightly on it. Fill the water pan with 1 liter of distilled, sterilized water. We recommend cleaning (chap. 22.1) and refilling the pans 2 to 3 times a week. For evacuation, remove the water pan. -
Page 30: Connecting The Co Gas Cylinder
4.4.1 Connecting the CO gas cylinder Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Escaping CO gas is heavier than air and accumulates at the ground or possibly in lower-lying parts of the building. -
Page 31: Gas Cylinder Connection Kit (Accessory)
Otherwise, the quick acting closure socket may leak, and/or it may become impossible to connect the original hose nozzle. In this case, please contact BINDER Service. To remove the connection, pull the hose nozzle off the quick acting closure socket. -
Page 32: Electrical Connection
• Only use original connection cables from BINDER according to the above specification. • Prior to connection and start-up, check the power supply voltage. Compare the values to the specified data located on the chamber’s type plate (centrally located at the bottom of the left-hand side of the... -
Page 33: Functional Overview Of The Rd4 Chamber Controller
“Set points” menu directly at the controller or use the APT-COM™ 4 Multi Management Software (option) specially developed by BINDER. The controller offers various notifications and alarm messages with visual and audible indication. All con- troller settings remain valid until the next manual change. They are stored also after turning off the chamber. -
Page 34: Menu Structure Of The Controller And Access Levels
• Admin: The password enables access to advanced controller functions and settings. Factory setting is 00 01. • Service: The password enables access to all controller functions (for BINDER Service only). As soon as a password has been assigned, access to the respective functions is blocked and only available after entering the correct password. -
Page 35: Performance During And After Power Failures And Shut Down
(chap. 22). WARNING: If customer should use a BINDER chamber running in non-supervised continu- ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible. -
Page 36: Preset Factory Parameters
1 min chap. 13.4 Password for “User” access level. chap. 10.2.1 Password for “Admin” access level. chap. 10.2.2 The following settings should only be modified by BINDER service: Humidity control 0 % r.h. chap. 14.1 Door heating Offset value 3.0 °C Adjustment factor of the heating power 12.0... -
Page 37: Altitude Of The Installation Site Above Sea Level
• Current time (chap. 12.4), format HH:MM: Use the arrow buttons to set the hours, continue with the OK button. Use the arrow buttons to set the minutes, confirm with the OK button Set the controller to the desired temperature, and CO set points that shall be used to operate the chamber (chap. -
Page 38: Temperature And Co
Temperature and CO set-point entry Required access level: “User”. Setting ranges Control ranges Temperature 0 °C / 32 °F up to 50 °C / 122 °F 6 °C / 10.8 °F above ambient up to 50 °C / 122 °F 0 vol.-% up to 20 vol.-% 0 vol.-% up to 20 vol.-% Temperature set-point entry... -
Page 39: Placing Samples In The Incubator
If no CO concentration is indicated (display showing “ – – – – ”) the CO sensor is not con- nected. Note when setting high gas concentrations Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. -
Page 40: Setting Special Controller Functions
Setting special controller functions In the “Functions on/off” menu you can define the switching state of the special controller functions. Required access level: “User”. Path: Normal display Setpoints Functions on/off The functions are displayed from right to left. Example: Function 1 “Idle mode” activated = 0001. Function 1 “Idle mode” deactivated = 0000. Submenu “Functions on/off”. -
Page 41: Deactivated Co 2 Control
Deactivated CO control When operating the chamber without a CO2 gas connection, you can deactivate CO2 control with the function “CO2 off” (chap. 9) to avoid alarms of the CO gas system. No CO tolerance range alarms and no pressure alarm will be emitted. The actual CO value continues to be displayed in Normal Display. -
Page 42: Assign And Modify A Password
10.2 Assign and modify a password In this menu you can assign and modify the passwords of the “User” and “Admin” access levels. Required access level: “Admin”. Keep the password well in mind. There is no access to the corresponding menu functions without the correct password. -
Page 43: Temperature Safety Devices
If one of the over temperature protective devices permanently turns off the chamber, the user cannot restart the device again. The protective cut-off devices are located internally. Only a service specialist can replace them. Therefore, please contact an authorized service provider or BINDER Service. 11.2 Overtemperature safety controller class 3.1 The chambers are regularly equipped with an electronic overtemperature safety controller (temperature safety device class 3.1 according to DIN 12880:2007). -
Page 44: Setting The Safety Controller Mode
11.2.1 Setting the safety controller mode Required access level: “User”. Normal display Setpoints Safety controller Mode Path: Press the OK button to enable the setting. Setting the safety controller mode The current setting flashes. Use the arrow buttons to select between LIMI (Limit) and OFFS (Offset). -
Page 45: Message And Measures In The State Of Alarm
11.2.3 Message and measures in the state of alarm The state of alarm is indicated visually in Normal display. If the buzzer is enabled (chap. 15.3) there is an additional audible alert. The heating turns off. As soon as the inner chamber temperature has cooled down below the safety controller value, the heating is released and temperature control continues. -
Page 46: General Controller Settings
General controller settings The general settings can be accessed in the “Settings” submenu, which is available for users with “Service” or “Admin” authorization level. It serves to enter date and time, select the language for the controller menus and the desired temperature unit and to configure the controller’s communication functions. The display of some network settings is available for all users in the “Chamber... -
Page 47: Setting The Current Date
12.3 Setting the current date Required access level: “Admin”. Following start-up of the chamber (chap. 6.3), it is “User”. Normal display Settings Chamber Date Path: Press the OK button to enable the setting. Setting the date: day The current setting flashes. Enter the current day with the arrow but- tons. -
Page 48: Setting The Current Time
12.4 Setting the current time Required access level: “Admin”. Following start-up of the chamber (chap. 6.3), it is “User”. Normal display Settings Chamber Time Path: Press the OK button to enable the setting. Setting the time: hours The current setting flashes. Enter the current hour with the arrow but- tons. -
Page 49: Setting The Chamber Address
12.6 Setting the chamber address This setting is required for the communication with the BINDER APT-COM™ 4 Multi Management Software. The chamber address settings in the chamber controller and in the software must be identical. Required access level: “Admin”. Path:... -
Page 50: Tolerance Range Settings
Tolerance range settings In this menu you can define for temperature and CO the deviation between the actual value and setpoint, which that shall cause a tolerance range alarm. The entered value defines the limit of permitted deviations from the set-point (exceeding and falling below). Reaching this limit triggers tolerance alarm. In addition you can specify a delay time for this alarm. -
Page 51: Setting The Co Tolerance Range
13.3 Setting the CO tolerance range Path: Normal display Settings Various CO2 range Press the OK button to enable the setting. Setting the CO tolerance range The current setting flashes. Enter the desired CO range with the ar- row buttons. Entry range: 1.0 vol.-% up to 10.0 vol.-% Confirm the entry with the OK button. -
Page 52: Chamber Settings (For Experienced Users Only)
+/- 5 % r.h. if required. Factory setting: 0 % r.h. If the ambient temperature deviates from the values recommended by the manufacturer, the conditions for maximum air humidity with condensation-free inner chamber are no longer guaranteed. Contact BINDER Service for assistance. Path: Normal display... - Page 53 Alarm indication (example: safety controller alarm) Safety controller Alarm messages overview: Moment of alarm message and Condition Alarm message switching the zero-voltage alarm relay output Icon after configurable time Open chamber door “Door open” (chap. 13.4). Factory setting: 1 minute Setpoint of the safety controller “Safety controller”...
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Page 54: Information Messages
• Confirmation while the state of alarm persists: Only the buzzer is muted. The visual alarm message continues to be displayed until the alarm condition is removed. Then it is reset automatically. • Confirmation after the alarm has ended: The buzzer and the visual alarm message are rest together. When operating the chamber without CO gas connection, turn off CO control (function... -
Page 55: Activating / Deactivating The Audible Alarm (Alarm Buzzer)
Normal display. 15.4 Measures in case of alarm Only qualified service personnel authorized by BINDER must perform repair. Repaired chambers must comply with the BINDER quality standards. 15.4.1 Door open alarm The open and closed position of the chamber door is controlled via the door contact switch. If the door is open, the temperature and CO controls turn off. -
Page 56: Temperature Tolerance Range Alarm (Overtemperature / Too Low Temperature)
• To decrease the temperature, proceed as follows: Turn off the chamber. Open both chamber doors for approx. 5 minutes. Turn on the chamber again. You can restart normal operation as soon as the re- quested values have equilibrated. If the same alarm recurs, please contact BINDER Service. CB-S / CB-S-UL (E7) 01/2025 page 56/115... -
Page 57: Co Tolerance Range Alarm (Co Over/Under Concentration)
• Open both chamber doors for approx. 30 seconds. Respect the precautions when working with CO (chap. 1.9). • Normal operation can be restarted, as soon as the requested values have been readjusted. • Reset the alarm message. If the same alarm recurs, please contact BINDER Service. 15.4.5 CO pressure alarm If CO control has been deactivated (chap. -
Page 58: Power Failure Alarm
• If these points do not reveal the source of the fault, it may be that the chamber is faulty. Please contact BINDER Service. The outlet pressure of the gas cylinder must be 2.0 bar / 29 psi above the ambient pressure. -
Page 59: Alarms Referring To Temperature Sensor Failures
• Turn off the chamber. • If necessary, clean and disinfect the chamber. Automatic sterilization is not possible with this fault. • Please contact BINDER Service. Failure of inner temperature sensor • Actual temperature value display shows “ – – – – ”... -
Page 60: Alarms Referring To Co Sensor Failure
• Turn off the chamber. • Remove the sensor (chap. 4.2), replace it if necessary. • If necessary, clean, disinfect and sterilize the chamber before restarting it • Please contact BINDER Service. sensor not connected • Actual CO value display shows “ – – – – ”... -
Page 61: Zero-Voltage Relay Alarm Output
15.5 Zero-voltage relay alarm output Collective alarm output via the zero-voltage relay alarm contact The chamber is equipped at the rear with a zero-voltage relay output (13) for temperature and CO , which permits the transmission of alarms to an external monitoring system in order to monitor and record the alarm signals. -
Page 62: Ethernet Network Settings
Ethernet network settings The settings of the “Ethernet” submenu are used for networking chambers with an Ethernet interface, e.g. to connect them with BINDER’s APT-COM™ 4 Multi Management Software (option, chap. 20.4). 16.1 Showing the network settings Required access level: “User”. -
Page 63: Showing The Subnet Mask
16.1.3 Showing the subnet mask Path: Normal display Chamber info Ethernet Subnet mask Display of the subnet mask (exam- ple) Toggle forth and back with the Back button and the OK button. Subnet mask 0.0.0.0 With the arrow-down button you can now change to the next parameter (standard gateway). With the Back button you can go back to the “Ethernet”... -
Page 64: Showing The Dns Chamber Name
16.1.6 Showing the DNS chamber name Normal display Chamber info Ethernet Path: chamber name Display of the DNS chamber name (example) Toggle forth and back with the Back button and the OK button. DNS chamber name BinderRD4-000cd80a28 With the Back button you can go back to the “Ethernet” submenu and, repeatedly pressing it, to Normal display. -
Page 65: Selecting The Type Of Assignment Of The Dns Server Address (Automatic / Manual)
16.2.2 Selecting the type of assignment of the DNS server address (automatic / manual) Access to this function is possible only if automatic IP address assignment has been selected (chap. 16.2.1). Path: Normal display Settings Ethernet DNS server Press the OK button to enable the setting. Selection of the type of assignment of the DNS server address. -
Page 66: Setting The Subnet Mask
IP address assignment (sample values). The forth section of the IP address is shown. Enter the desired value with the arrow buttons. Confirm the setting with the OK button. IP address With the arrow-down button you can now change to the enter the subnet mask. With the Back button you can go back to the “Ethernet”... -
Page 67: Assigning The Dns Server Address
(chap. 17.3). Temperature values are always given out in °C. • Chamber data for BINDER Service “DL2” This data is intended for use by BINDER Service. The storage rate is fix (1 minute). Temperature values are always given out in °C. -
Page 68: Storage Capacity
(chap. 18.2) and when loading a new firmware version by BINDER service, the entire data memory is deleted. BINDER service can also install the configuration by means of a setup program without deleting the data. Regardless of this, BINDER Service can delete the data via a setup program. -
Page 69: Usb Menu: Data Transfer Via Usb Interface
For detailed information on the file content see chap. 17.1. • Service data The "Service" folder is created on the USB stick and can be sent to BINDER Service. In addition to the configuration and recorder data, it contains further service-relevant information. -
Page 70: Export Functions
18.3 Export functions Required access level: any user Function “Export configuration”. To write the configuration data from the controller to the USB stick, press the OK button. Export configuration With the arrow-down button you can now change to the next function. Function “Export recorder data”. -
Page 71: Removing The Usb Stick
You need only use an accurate pH indicator or a pH- measuring electrode, which are standard equipment in cell culture laboratories. This method is not suitable for calibrating the BINDER FPI sensor system. This method is based on the acid base equilibrium of the buffer system in the culture media. -
Page 72: Measuring Co 2 Directly Via Chemical Indicator Tubes
All the necessary equipment must be supplied by one manufacturer only and in a defined test system. Note: These test systems are not very accurate. A typical accuracy is around 10% of the full-scale value. These test systems are not suitable for calibrating the BINDER FPI sensor system. CB-S / CB-S-UL (E7) 01/2025... -
Page 73: Measuring Co Directly With An Electronic Infrared Measuring Device
CO incubators. The CTM 01 is suitable both for reference measurements in certified laboratories, and for service purposes. Please contact the BINDER INDIVIDUAL team. 19.2 Temperature reference measurement When performing a temperature reference measurement using an electronic measuring, and temperature display device, it is important to use a device traceable to an acknowledged standards/calibration institution (DKD, PTB for Germany) with valid calibration certificate. -
Page 74: Options And Accessories
Options and accessories 20.1 Silicone access ports 30 mm / 1.18 in, closable with 2 silicone plugs (8012-0558 rear, 8012-0559 left, 8012-0560 right) (option) Figure 19: Positions of the optional silicon access ports left (a), rear (b), and right (c) A warning sticker is located above each access port. -
Page 75: Base On Castors (Accessory)
This could happen e.g. while opening or closing the door, cleaning, loading and unloading the chamber. BINDER offers a flat stacking frame for safe stack- ing of two incubators. -
Page 76: Avoiding Microbial Contamination
Avoiding microbial contamination The main types of microbial contaminants in cell and tissue culture are bacteria, fungi, yeast, mycoplasma, and viruses. This chapter gives an overview of potential sources of contamination and precautions and measures to eliminate them. 21.1 Cells and media •... -
Page 77: Chamber Design And Equipment
• Condensation in the inner chamber represents a particular risk of contamination. The humidifying sys- tem with water pan with integrated condensation point developed by BINDER is an effective and easy way to ensure high humidity (95 +/- 2% r.h.) inside the incubator without any condensation forming on the inner surfaces. -
Page 78: Handling The Co Incubator
21.5 Handling the CO incubator Any manipulation of the CO incubator involves some contamination risks, from installation to opening of the doors and regular cleaning. Installation away from sources of contamination • Do not place the CO incubator on the floor or close to windows and doors. Use the optional stand, if appropriate. -
Page 79: Cleaning, Decontamination / Disinfection, And Sterilization
Cleaning, decontamination / disinfection, and sterilization Clean the chamber after each use in order to prevent potential corrosion damage by ingredients of the charging material. Prior to renewed startup, allow the chamber to completely dry after all cleaning and decontamination measures. - Page 80 Do not use cleaning agents that may cause a hazard due to reaction with components of the device or the charging material. If there is doubt regarding the suitability of cleaning products, please contact BINDER service. We recommend using the neutral cleaning agent Art. No. 1002-0016 for a thorough cleaning.
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Page 81: Decontamination / Chemical Disinfection Of The Chamber
For chemical disinfection, we recommend the disinfectant spray Art. No. 1002-0022. Any corrosive damage that may arise following use of other disinfectants is excluded from lia- bility by BINDER GmbH. With every decontamination / disinfection method, always use adequate personal safety con- trols. -
Page 82: Disinfection Of The Co 2 Sensor
22.3 Disinfection of the CO sensor To ensure complete disinfection and proper function of the sensor, BINDER recommends a wipe disinfec- tion of the sensor head with pure alcohol or non-corrosive alcohol-based surface disinfectants. The disin- fectant must be non-corrosive and free of chlorine or any acid. We recommend using the disinfectant Art. -
Page 83: Hot-Air Sterilization
Hot-air sterilization The very first sterilization after operation may cause an odor. This is not a quality defect. We recommend ventilating well the room during sterilization. 23.1 Overview Heating up Holding Cooling down Equilibrating 180 °C 37 °C 4 hours 6 hours unlimited Sterilization cycle of 10 hours... -
Page 84: Starting And Running The Hot-Air Sterilization Cycle
The CO sensor head was especially adjusted for your specific chamber. To avoid confusion, an adhe- sive label with a serial number is adhered to the sensor head. When exchanging the sensor, repeat CO control adjustment. NOTICE Danger of confusing CO sensors. -
Page 85: Performance During Sterilization
“Start sterilization” submenu The current setting flashes. Select with the arrow buttons between YES (start sterilization) and NO (do not start sterilization). To start the sterilization cycle, select “YES” and press the OK button to confirm. Start sterilization After selecting “NO”: The controller changes back to the “Sterilization” menu. After selecting “YES”: Sterilization starts and the controller changes to Normal display. -
Page 86: Completing The Entire Sterilization Cycle
23.3.3 Completing the entire sterilization cycle The effective sterilization phase (heating up and holding phase) is automatically finished after 4 hours. Now the information message “Sterilization success” indicates the successful sterilization. This is followed by a defined 6-hour cooling-down phase until 37 °C is reached. The entire sterilization cycle ends after a total of 10 hours. -
Page 87: Premature Termination After More Than 4 Hours, I.e., During The Cooling-Down Phase: Successful Sterilization
23.4.2 Premature termination after more than 4 hours, i.e., during the cooling-down phase: successful sterilization The duration of the entire sterilization is 10 hours. If you want to shorten the sterilization procedure in order to save time, you can prematurely terminate it during the cooling-down phase, i.e. no sooner than after 4 hours. -
Page 88: Opening The Outer Door
• Do not open the chamber doors until the interior temperature has dropped to 37 °C / 98.6 °F. • After premature termination the chamber reverts to its standard operational status. • The CO actual value display shows “ – – – – ” because the CO sensor is disconnected. -
Page 89: Maintenance, And Service, Troubleshooting, Repair, Testing
For personnel requirements please refer to the Service Manual. • Repair Repair of the chamber can be performed by BINDER Service or by BINDER qualified service partners or technicians, in accordance with the description in the Service Manual. After maintenance, the chamber must be tested prior to resuming operation. -
Page 90: Maintenance Intervals, Service
When exchanging the sensor, you must repeat the CO adjustment. We recommend taking out a maintenance agreement. Please consult BINDER Service. BINDER telephone hotline: +49 (0) 7462 2005 555 BINDER fax hotline:... -
Page 91: Gas Inlet Fine Filter
If there are is a technical fault or shortcoming, take the chamber out of operation and inform BINDER Service. If you are not sure whether there is a technical fault, proceed ac- cording to the following list. If you cannot clearly identify an error or there is a technical fault, please contact BINDER Service. -
Page 92: Temperature
No. Fault description Possible cause Required measures Pt 100 sensor defective. Chamber does not heat up. Heating element defective. Contact BINDER service. Semiconductor relay defective. Chamber is heating perma- nently. Set-point is not re- Semiconductor relay defective. Contact BINDER service. -
Page 93: Co 2
Transfer the cultures to another The pH indicator of the cell sensor system defective. incubator and contact BINDER medium changes its normal Service. color Recovery time (up to 5 vol.- % CO ) after doors were sensor system defective. -
Page 94: Humidity
Condensations inside the Doors not closed. Close door properly. chamber. Door gaskets defective. Replace door gaskets. Place the chamber on a BINDER Chamber placed on very cold stand to increase the distance to floor. the floor. Doors not closed. Close door properly. -
Page 95: Sterilization
24.6 Sending the chamber back to BINDER GmbH If you return a BINDER product to us for repair or any other reason, we will only accept the product upon presentation of an authorization number (RMA number) that has previously been issued to you. An au- thorization number will be issued after receiving your complaint either in writing or by telephone prior to your sending the BINDER product back to us. -
Page 96: Disposal
Disposal 25.1 Disposal of the transport packing 25.1.1 Outer chamber packing Packing element Material Disposal Straps to fix packing on pallet Plastic Plastic recycling (no image) Shipping box Cardboard Paper recycling Edge stuffing, top PE foam Plastic recycling PE foam Plastic recycling Pallet with foamed plastic stuffing... -
Page 97: Decommissioning
In this case, clean and dry the incubator running at 37 °C / 98.6 °F with doors open for at least one hour before loading it with samples. BINDER recommends performing a hot air sterilization of the chamber before commissioning. -
Page 98: Disposal Of The Chamber In The Member States Of The Eu Except For The Federal Republic Of Germany
According to Annex I of Directive 2012/19/EU of the European Parliament and of the Council on waste electrical and electronic equipment (WEEE), BINDER devices are classified as “monitoring and control instruments” (category 9) only intended for professional use”. They must not be disposed of at public col- lecting points. -
Page 99: Disposal Of The Chamber In Non-Member States Of The Eu
Danger of violation against existing law if not disposed of properly. Failure to comply with applicable law. Alteration of the environment. For final decommissioning and disposal of the chamber, please contact BINDER ser- vice. Follow the statutory regulations for appropriate, environmentally friendly disposal. -
Page 100: Technical Description
December 1996 by TÜV CERT). All test equipment used is subject to the administration of measure- ment and test equipment that is also a constituent part of the BINDER QM DIN EN ISO 9001 systems. They are controlled and calibrated to a DKD-Standard at regular intervals. -
Page 101: Cb-S / Cb-S-Ul Technical Data
The technical data refers to the so defined usable volume. Do NOT place samples outside this usable volume. Do NOT load this volume by more than half to enable sufficient airflow inside the CO incuba- tor. Do NOT divide the usable volume into separate parts with large area samples. Do NOT place samples too close to each other in order to permit circulation between them and thus obtain a homogenous distribution of temperature and CO 26.4 CB-S / CB-S-UL technical data... -
Page 102: Important Conversion Data For Non-Si Units
+22 °C +/- 3 °C / 71.6 °F +/- 5.4 °F and a power supply voltage fluctuation of +/-10%. The technical data is determined in accordance to BINDER Factory Standard Part 2:2015 and DIN 12880:2007. All indications are average values, typical for chambers produced in series. We reserve the right to change technical specifications at any time. -
Page 103: Conversion Table For Gas Inlet Pressures, Bar - Psi
65.2 26.7 Equipment and options (extract) To operate the chamber, use only original BINDER accessories or accessories / components from third-party suppliers authorized by BINDER. The user is responsible for any risk arising from using unauthorized accessories. Regular equipment Microprocessor controller RD4 for temperature and CO... -
Page 104: Options, Accessories, And Spare Parts (Extract)
26.8 Options, accessories, and spare parts (extract) BINDER GmbH is responsible for the safety features of the chamber only, provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts. -
Page 105: Dimensions
26.9 Dimensions CB-S / CB-S-UL 170: [Dimensions in mm] CB-S / CB-S-UL (E7) 01/2025 page 105/115... - Page 106 CB-S / CB-S-UL 260: [Dimensions in mm] CB-S / CB-S-UL (E7) 01/2025 page 106/115...
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Page 107: Certificates And Declarations Of Conformity
Certificates and declarations of conformity 27.1 EU Declaration of Conformity CB-S / CB-S-UL (E7) 01/2025 page 107/115... - Page 108 CB-S / CB-S-UL (E7) 01/2025 page 108/115...
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Page 109: Ukca Declaration Of Conformity
27.2 UKCA Declaration of Conformity CB-S / CB-S-UL (E7) 01/2025 page 109/115... -
Page 110: Contamination Clearance Certificate
Contamination clearance certificate Unbedenklichkeitsbescheinigung 28.1 For chambers located outside the USA and Canada Declaration regarding safety and health Erklärung zur Sicherheit und gesundheitlichen Unbedenklichkeit The German Ordinance on Hazardous Substances (GefStofV), and the regulations regarding safety at the workplace require that this form be filled out for all products that are returned to us, so that the safety and the health of our employees can be guaranteed. - Page 111 Kind of transport / transporter / Transportweg/Spediteur: Transport by (means and name of transport company, etc.) Versendung durch (Name Spediteur o.ä.) __________________________________________________________________________________ Date of dispatch to BINDER GmbH / Tag der Absendung an BINDER GmbH ___________________________________________________________________________________ CB-S / CB-S-UL (E7) 01/2025 page 111/115...
- Page 112 We hereby commit ourselves and guarantee that we will indemnify BINDER GmbH for all damages that are a consequence of incomplete or incorrect information provided by us, and that we will exempt BINDER GmbH from eventual damage claims by third parties./ Wir versichern, dass wir gegenüber BINDER für jeden...
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Page 113: For Chambers In The Usa And Canada
Please complete this form and the Customer Decontamination Declaration (next 2 pages) and attach the required pictures. E-mail to: IDL_SalesOrderProcessing_USA@binder-world.com After we have received and reviewed the complete information we will decide on the issue of a RMA num- ber. Please be aware that size specifications, voltage specifications as well as performance specifications are available on the internet at www.binder-world.us... - Page 114 Customer (End User) Decontamination Declaration Health and Hazard Safety declaration To protect the health of our employees and the safety at the workplace, we require that this form is com- pleted by the user for all products and parts that are returned to us. (Distributors or Service Organizations cannot sign this form) NO RMA number will be issued without a completed form.
- Page 115 4.5 Shipping laws and regulations have not been violated. I hereby commit and guarantee that we will indemnify BINDER Inc. for all damages that are a con- sequence of incomplete or incorrect information provided by us, and that we will indemnify and hold harmless BINDER Inc.
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