Cb (e6.1)
co2 - incubators
co2 – incubators with o2 control
with sterilizable ndir sensor system for co2
and microprocessor controller t4.12 (145 pages)
Model version: bd056-230v;bd056ul-120v;bd115-230v;bd115ul-120v;bd260-230v;bd260ul-120v;bf056-230v;bf056ul-120v;bf115-230v;bf115ul-120v;bf260-230v;bf260ul-120v;ed056-230v;ed056ul-120v;ed115-230v;ed115ul-120v;ed260-230v;ed260ul-240v;fd056-230v;fd056ul-120v; (99 pages)
Model version:kbf115-230v;kbf115-240v;kbf240-230v;kbf240-240v;kbf720-230v;kbf720-240v;kbf1020-230v;kbf1020-240v;kmf115-230v;kmf115-240v;kmf240-230v;kmf240-240v;kmf720-230v;kmf720-240v.
constant climate chambers with program control.constant climate chamb (155 pages)
Summary of Contents for Binder C 170
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Operating Manual C 170 (E3) Incubator with FPI-sensor system and display controller RP1 Model Model version Art. No. C 170 C170-230V-R 9040-0112, 9140-0112 C 170-UL C170-120V-R 9040-0113, 9140-0113 BINDER GmbH Address Post office box 102 D-78502 Tuttlingen Tel. +49 7462 2005 0...
Contents SAFETY ........................5 Legal considerations ........................... 5 Structure of the safety instructions ...................... 5 1.2.1 Signal word panel ........................5 1.2.2 Safety alert symbol ........................6 1.2.3 Pictograms ..........................6 1.2.4 Word message panel structure ....................7 Localization / position of safety labels at the unit ................7 Type plate ............................
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16. MAINTENANCE, AND SERVICE ................. 70 16.1 Maintenance intervals, service ......................70 16.2 Checking the air jacket heating fan ....................70 16.3 Gas inlet fine filter ..........................70 16.4 Sending the unit back to BINDER GmbH ..................71 C (E3) 08/2015 page 3/93...
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19.5 Important conversion data for non-SI units ..................81 19.6 Conversion table for gas inlet pressures, bar – psi ................81 19.7 Equipment and options C 170 ......................82 19.8 Accessories and spare parts ......................83 19.9 Dimensions C 170 ..........................84 20.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use and safety during operation and maintenance. In no event shall BINDER be held liable for any dam- ages, direct or incidental arising out of or related to the use of this manual.
WARNING Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irreversible) injury CAUTION Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury CAUTION Indicates a potentially hazardous situation, which, if not avoided, may result in damage to the product and/or its functions or to property in its proximity.
Risk of injury (UL units only). Observe the safety instructions in the operating manual. Figure 1: Position of labels Figure 2: Position of labels on the CO incubator C 170 on the CO incubator C 170-UL C (E3) 08/2015 page 7/93...
Keep safety labels complete and legible. Replace safety labels that are no longer legible. Contact BINDER Service for these replacements. Type plate Figure 3: Position of type plate Nominal temp. 190 °C 1,10 kW / 6,1 A 374 °F 230 V / 50 Hz...
(for Germany). BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or quali- fied personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
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The CO incubator C 170 does not dispose of any measures of explosion protection. DANGER Explosion hazard. Danger of death. ∅ Do NOT introduce any substance into the CO incubator which is combustible or explo- sive at working temperature. ∅ NO explosive dust or air-solvent mixture in the inner chamber.
Precautions when working with CO Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation or a suitable connection to an exhaust system. We recommend installing a CO warning system.
Intended use incubators C 170 are suitable for the cultivation of mammal cells under typical conditions of approx. 37 °C / 98.6 °F. The incubator permits setting defined pH conditions by common NaHCO buffer systems of commercial cell media by keeping an exact CO atmosphere inside.
A highly precise, drift-free CO infrared measuring system in combination with the permanent mixture of gas through a special proprietary gas mixing head developed by BINDER allows precise and con- stant CO concentrations for long periods. This creates optimum growth conditions for cultures. The CO sensor can be removed from the inner chamber by hand and cleaned with suitable detergents if needed.
Unit overview Figure 5: CO incubator C 170, closed Triangle instrument panel with RP1 controller for temperature and CO Door handle Main power switch C (E3) 08/2015 page 14/93...
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(11) (10) Figure 6: CO incubator C 170, outer door open Glass door handle Water pan Shelves Shelf holder Pt 100 temperature sensor sensor (10) Gas mixing head for CO (11) Silicone measuring port in the glass door C (E3) 08/2015...
Unit doors The outer door of the C 170 is equipped with a heater on its inner side. The door must be closed while the unit is operating normally in order to ensure stable climatic conditions in the inner chamber.
Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly. Second-hand units are marked with a sticker on the unit door. Please remove the sticker before commis- sioning the unit.
The chambers are designed for setting up inside a building (indoor use). Freestanding C 170 incubators are suitable for installation on tables or on the optionally available stand. Note: The site of installation must be capable of supporting the unit’s weight (see technical data, chap.
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For the user there is no risk of temporary overvoltages in the sense of EN 61010-1:2010. Do not install or operate the CO incubator C 170 in potentially explosive areas. DANGER Explosion hazard.
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An example of how to evaluate laboratory volume and air change rate: Question: Is an air change rate of 1/h sufficient for a lab with a volume of 100 cubic meters / 3,531.5 cubic feet with 10 incubators C 170, opened 4 times per hour? Calculation: CO...
Installation and connections Shelf holder and shelves The shelf holder consists of two identical side parts. Insert them alongside the lateral walls of the inner chamber. They are fixed by cams on the bottom of the inner chamber. For assembly and disassembly, fold both side parts towards the middle.
Permitted shelf loads: Maximum load on one single shelf: 10 kg / 22 lb Maximum total load on all shelves: 30 kg / 66 lb sensor 4.2.1 Connecting the CO sensor Turn off the unit. Open the door of the inner chamber and plug the CO sensor (4) into the permanently installed holding tube located in the upper part of the rear of the inner chamber.
The CO sensor head was especially adjusted for the specific chamber. To avoid confusion, an adhesive label with a serial number is adhered to the sensor head. When exchanging the sensor, you must repeat the CO adjustment. CAUTION Different CO sensor.
Make sure that the water pan has firm contact with the inner chamber bottom and rests tightly on it. Fill the water pan with 1 liter of distilled, sterilized water. We recommend cleaning (chap. 15.1) and refilling the pans 2 to 3 times a week. For evacuation, remove the water pan.
4.4.1 Connecting the CO gas cylinder Carbon dioxide (CO ) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO gas that may escape via good room ventilation or a suitable connection to an exhaust system. We recommend installing a CO warning system.
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Only use the supplied hose nozzle to connect to the quick acting closure socket. Otherwise, the quick acting closure socket may leak, and/or it may become impossible to con- nect the original hose nozzle. In this case, please contact BINDER Service. Remove the rubber cover (b) by pulling it off.
4.4.3 Gas cylinder connection kit (option) The gas cylinder connection kit for CO (Art. no. 8012-0014) includes the following parts for connecting a gas cylinder to the CO incubator: • Pressure reducer with manometers for cylinder pressure (high pressure gauge) and outlet pressure (low pressure gauge) •...
Electrical connection The CO incubators C 170 are supplied ready for connection. They come with an IEC connector plug. Current Nominal voltage ± 10% at the Model Power plug Unit fuse indicated power frequency type 230 V at 50 Hz...
Start up After connecting the supply lines, turn on the unit by the main power switch (2). After turning on the incubator for the first time, enter the altitude of the site above sea level into the con- troller RP1 (chap. 8.1). Warming chambers may release odors in the first few days after commissioning.
Charge the incubator only after equilibration of temperature and CO Check that the interior of the C 170 is empty, including the trays and the water pan. If you do not know what the C 170 was last used for, for hygiene purposes you should clean and disinfect or sterilize the interior (chap.
After a few seconds, the upper display shows the current interior temperature of the C 170 and the lower display shows the current CO interior concentration.
In operating modes HAND and USER, access the next operating function by pressing “MODE”. Pressing “MODE” will confirm the changed value of the current operating function, i.e., the C 170 will from now on regulate at the new value.
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Temperature set point 0.0 °C to 50.0 °C Must be at least 7 °C / 12.6°F above the ambient temperature in order to ensure a stable temperature inside the C 170. set point 0.0 vol.-% to 20.0 Common value: 5.0 vol.-% (factory setting), or depending on the NaHCO vol.-%...
Operating mode USER: Advanced settings • In Normal display, press “MODE” and “EXIT” simultaneously for 3 seconds to access the options for selecting the C 170's operating modes. Figure 18: Selecting the operating mode • Press “▲”until the value “OP.U” (operating mode USER) appears in the upper display.
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Temperature set point 0.0 °C to 50.0 °C Must be at least 7 °C / 12.6 °F above the ambient temperature in order to ensure a stable temperature inside the C 170. set point 0.0 vol.-% to 20.0 Common value: 5.0 vol.-% (factory setting), or depending on the NaHCO vol.-%...
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When exceeded, the safety controller triggers an alarm. For tech- nical reasons, the offset value should be at least 2 °C (factory setting). Inhibit time. This operating function is not required to operate the C 170. ----- Its value should always be zero.
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Display Setting range Operating function Firmware revision of safety controller for service / maintenance pur- poses. Data record (year) for service / maintenance purposes. Data record (month) for service / maintenance purposes. Data record (day) for service / maintenance purposes. Data set version for service / maintenance purposes.
Operating mode LOCK: Locking/unlocking of the operating functions’ set- tings by operating mode HAND To avoid operating functions being changed by unauthorized persons, you can lock the operating func- tions’ settings. • In operating mode HAND, define a 3-digit numeric password in operating function “PA.H”. Locking the operating functions of operating mode HAND: •...
• Enter the password using the “▲” and “▼”buttons. • Confirm the entry with “MODE”. The controller returns to Normal display. If you have selected any password other than zero, operating mode HAND is temporarily unlocked. Changing the operating functions in operating mode HAND is now possible until a period of 30 sec. has passed with no activity.
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Toggle to operating mode USER Operating mode HAND Operating mode USER If operating mode HAND appears, toggle to operating mode USER with the arrow keys. Password request Password entry (factory setting: “1”). Entry of altitude Entry of the current altitude [km] above sea level with arrow keys. After 30 seconds the controller reverts to Normal Display automatically.
CO gas must be able to escape first. Open both doors of the unit for this purpose. If the CO concentration is indicated as “-0.4”, the CO sensor is defective. Please contact BINDER Service. C (E3) 08/2015 page 41/93...
If one of the over temperature protective devices permanently turns off the unit, the user cannot restart the device again. The protective cut-off devices are located internally. Only a service specialist can re- place them. Therefore, please contact an authorized service provider or BINDER Service. Safety controller (temperature safety device class 3.1) The incubator is equipped with an over temperature safety device class 3.1 acc.
The settings of the safety controller are inactive during sterilization (chap. 15.4). They become functional again following abortion of the sterilization and/or the restart of the unit at the main power switch. 9.2.1 Setting the safety controller set point type Select the safety controller set point type in operating mode USER (chap.
12 x Selecting the set point type Selecting set point type “Limit” Selecting set point type “Offset” Change using the arrow keys Select the desired set point type. After 30 seconds the controller reverts to Normal Display automatically. 9.2.2 Setting the safety controller set point You can check and set the safety controller set point in operating modes HAND (chap.
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Toggle to operating mode USER Operating mode HAND Operating mode USER If operating mode HAND appears, toggle to operating mode USER with the arrow keys. Password request Password entry (factory setting: “1”). 10 x Depending on the selected set point type (chap. 9.2.1) a different display appears: Entry of limit value or offset value With set point type Limit: With set point type Offset:...
Except for the tolerance range alarms, all alarms are displayed immediately when the fault occurs. The tolerance range alarms are suppressed for a selected time (delay times set for temperature alarm and for alarm) after opening the unit door or turning on the C 170. 10.2 Resetting the alarm messages •...
Connection to an external monitoring system To ensure short-circuit-proof alarm monitoring that will trigger the alarm when the C 170 is connected to an external alarm monitor, connect the external alarm monitoring system to the C 170 via the contacts 1 and 3.
≥ 2 °C. If necessary, adjust the rele- vant value. • Check whether samples were inserted into the C 170 that produce heat under the climatic conditions in the unit.
• Check the door's seals for any damage. Replace any damaged seals. Temperature too high (over temperature alarm): • Check whether samples were inserted into the C 170 that produce heat under the climate conditions in the unit. • Check the ambient conditions.
• Open both unit doors for approx. 30 seconds. Respect the precautions when working with CO (chap. 1.6). • Normal operation can be restarted, as soon as the requested values have been readjusted. If the same alarm recurs, please contact BINDER Service. 10.8 CO pressure too low The CO primary pressure at the intake valve is less than 0.3 bar / 4.4 psi below the ambient air pressure.
• Switching the zero-voltage relay alarm output Actions: • Turn off the C 170. • If necessary, clean and disinfect the C 170. Automatic sterilization is not possible with this fault. • Please contact BINDER Service. C (E3) 08/2015 page 51/93...
• Replace the power plug. • Turn the C 170 on with the main power switch. • If the fault shows again, the unit could be faulty. Please contact BINDER Service. Repair must only be performed by qualified service personnel authorized by BINDER.
You need only use an accurate pH indicator or a pH-measuring electrode, which are standard equipment in cell culture laboratories. This method is not suitable for calibrating the BINDER FPI sensor system. This method is based on the acid base equilibrium of the buffer system in the culture media.
Pin the end with the higher end of the scale to the adapter of the hand pump that belongs to that test system. Pin the other end through the silicone access port of the inner chamber door of the C 170 incuba- tor.
CO incubators. The CTM 01 is suitable both for reference measurements in certified laboratories, and for service purposes. Please contact the BINDER INDIVIDUAL team. 12.2 Temperature reference measurement When performing a temperature reference measurement using an electronic measuring, and temperature display device, it is important to use a device traceable to an acknowledged standards/calibration institu- tion (DKD, PTB for Germany) with valid calibration certificate.
A warning sticker is located above each access port. When operating a C 170 with silicon access ports, both silicon plugs must tightly close the access ports. If the plugs are inserted in a not-gastight manner, or if plugs are missing, CO gas may escape into the environment.
Avoiding microbial contamination The main types of microbial contaminants in cell and tissue culture are bacteria, fungi, yeast, mycoplas- ma, and viruses. This chapter gives an overview of potential sources of contamination and precautions and measures to eliminate them. 14.1 Cells and media •...
Hot air sterilization at 180 °C / 356 °F • The heating system of the C 170 permits hot-air auto-sterilization (chap. 15.4). Thus, a temperature of 180 °C / 356°F is maintained for a 30-minute period on all internal surfaces during its cycle, resulting in sterilization of the entire inner chamber.
14.5 Handling the CO incubator C 170 Any manipulation of the CO incubator involves some contamination risks, from installation to opening of the doors and regular cleaning. Installation away from sources of contamination • Do not place the CO incubator on the floor or close to windows and doors. Use the optional stand, if appropriate.
BINDER service. We recommend using the neutral cleaning agent Art. No. 1002-0016 for a thorough cleaning. Any corrosive damage that may arise following use of other cleaning agents is excluded from liability by BINDER GmbH. CAUTION Danger of corrosion. Damage to the unit.
We recommend using the disinfectant spray Art. No. 1002-0022. For chemical disinfection, we recommend the disinfectant spray Art. No. 1002-0022. Any corrosive damage that may arise following use of other disinfectants is excluded from liability by BINDER GmbH. C (E3) 08/2015 page 61/93...
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With every decontamination / disinfection method, always use adequate personal safety con- trols. In case of contamination of the interior by biologically or chemically hazardous material, there are two possible procedures depending on the type of contamination and charging material. 1.
15.3 Disinfection of the CO sensor To ensure complete disinfection and proper function of the sensor, BINDER recommends a wipe disinfec- tion of the sensor head with pure alcohol or non-corrosive alcohol-based surface disinfectants. The disin- fectant must be non-corrosive and free of chlorine or any acid. We recommend using the disinfectant Art.
Sterilization cycle of approx. 12 hours Figure 28: Set-point profile during the sterilization cycle The C 170 can perform an automatically controlled hot-air sterilization cycle. This procedure will take approx. 12 hours and consists of the following steps: • Heating up phase: The incubator heats up the inner chamber as fast as possible to the sterilization set-point temperature •...
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Damage to the unit. Empty the water pan before starting the hot-air sterilization. • Clean the C 170. • Water pan, shelf holder, and shelves must be inside the incubator, the water pan at its usual place on the bottom.
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Figure 29: Initial sterilization display No sterilization can be started if the CO sensor is still plugged-in. You cannot start the sterilization if the operating mode HAND is locked. This is displayed by an error message. If this happens, unlock the operating mode HAND (chap. 7.4).
CAUTION The glass door and inner chamber become hot during sterilization. Danger of burning. ∅ Do NOT touch the glass door and inner surfaces during sterilization. CAUTION Interruption of the temperature reaction time. Ineffective sterilization. ∅ Do NOT open the unit doors during sterilization. •...
The upper display toggles from “ON” to “OFF“. • Press “MODE” to end the sterilization cycle. • Do not open the doors of the C 170 until the interior temperature has dropped to 37 °C / 98.6°F. C (E3) 08/2015...
• Do not open the doors of the C 170 until the interior temperature has dropped to 37 °C / 98.6 °F. • After turning on again the C 170, it reverts to its standard operational status. The indication “ - - - “ in the lower display shows that the CO sensor is disconnected.
Service personnel authorized by BINDER will check this filter for pollution at each maintenance interval and replace it, if appropriate, but at least once a year.
16.4 Sending the unit back to BINDER GmbH If you return a BINDER product to us for repair or any other reason, we will only accept the product upon presentation of an authorization number (RMA number) that has previously been issued to you. An authorization number will be issued after receiving your complaint either in writing or by telephone prior to your sending the BINDER product back to us.
Disposal 17.1 Disposal of the transport packing 17.1.1 Outer unit packing Packing element Material Disposal Straps to fix packing on pallet Plastic Plastic recycling (no image) Shipping box Cardboard Paper recycling Edge stuffing, top PE foam Plastic recycling PE foam Plastic recycling Pallet with foamed plastic stuffing...
In this case, clean and dry the incubator running at 37 °C / 98.6 °F with doors open for at least one hour before loading it with samples. BINDER recom- mends performing a hot air sterilization of the unit before commissioning.
(category 9) only intended for professional use“. They must not be disposed of at public collecting points. The CO incubator C 170 bears the symbol for the marking of electrical and electronic equipment manufactured / placed on the market in the EC after 13 August 2005. It must be disposed of in separate collection according to the directive 2002/96/EC on waste electrical and electronic equipment (WEEE).
Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling according to directive 2002/96/EC. The devices must be free from toxic, infectious or radioactive sub- stances in order to eliminate any health hazards to the employees of the recycling companies.
120 V / 230 V. Nominal temperature exceeded by 10 °C due to unit failure. Over Unit without function. Contact BINDER service. temperature protective device (class 1) responds (chap. 9.1). The miniature fuse for overcur- Check unit fuse and replace it if rent protection has triggered appropriate.
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Check the tube system for dirt alarm). Gas hose is dirty or obstructed. accumulation or obstruction, clean or replace it. Pressure sensor system defective Contact BINDER service. Doors not closed. Close door properly. Door gaskets defective. Replace door gaskets. cylinder is not connected Correctly connect the gas cylinder.
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Sterilization program started with Pull the CO sensor and remove it the CO sensor still plugged-in. from the incubator. Only qualified service personnel authorized by BINDER must perform repair. Repaired units must comply with the BINDER quality standards. C (E3) 08/2015 page 78/93...
December 1996 by TÜV CERT). All test equipment used is subject to the administration of measurement and test equipment that is also a constituent part of the BINDER QM DIN EN ISO 9001 systems. They are controlled and calibrated to a DKD-Standard at regular intervals.
19.4 C 170 technical data Exterior dimensions Width mm / inch 740 / 29.13 Height (incl. feet) mm / inch 870 / 34.25 Depth mm / inch 715 / 28.15 Depth including door handle, I-triangle, and mm / inch 795 / 31.30...
BINDER Factory Standard Part 1:2015 following DIN 12880:2007. The other technical data is determined in accordance to BINDER Factory Standard Part 2:2015 and DIN 12880. All indications are average values, typical for units produced in series. We reserve the right to change technical specifications at any time.
19.7 Equipment and options C 170 To operate the CO incubator, use only original BINDER accessories or accessories / compo- nents from third-party suppliers authorized by BINDER. The user is responsible for any risk arising from using unauthorized accessories. Regular equipment...
19.8 Accessories and spare parts BINDER GmbH is responsible for the safety features of the unit only, provided skilled electri- cians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
Contamination clearance certificate Unbedenklichkeitsbescheinigung 22.1 For units located outside North America and Central America Declaration regarding safety and health Erklärung zur Sicherheit und gesundheitlichen Unbedenklichkeit The German Ordinance on Hazardous Substances (GefStofV), and the regulations regarding safety at the workplace require that this form be filled out for all products that are returned to us, so that the safety and the health of our employees can be guaranteed.
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Kind of transport / transporter / Transportweg/Spediteur: Transport by (means and name of transport company, etc.) Versendung durch (Name Spediteur o.ä.) __________________________________________________________________________________ Date of dispatch to BINDER GmbH / Tag der Absendung an BINDER GmbH ___________________________________________________________________________________ C (E3) 08/2015 page 89/93...
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We are aware that, in accordance with Article 823 of the German Civil Code (BGB), we are directly liable with regard to third parties, in this instance especially the employees of BINDER GmbH, who have been entrusted with the handling / repair of the unit / component. / Es ist uns bekannt, dass wir gegenüber Dritten –...
Please complete this form and the Customer Decontamination Declaration (next 2 pages) and attach the required pictures. E-mail to: IDL_SalesOrderProcessing_USA@binder-world.com After we have received and reviewed the complete information we will decide on the issue of a RMA number. Please be aware that size specifications, voltage specifications as well as performance specifi- cations are available on the internet at www.binder-world.us...
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Customer (End User) Decontamination Declaration Health and Hazard Safety declaration To protect the health of our employees and the safety at the workplace, we require that this form is com- pleted by the user for all products and parts that are returned to us. (Distributors or Service Organizations cannot sign this form) NO RMA number will be issued without a completed form.
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4.5 Shipping laws and regulations have not been violated. I hereby commit and guarantee that we will indemnify BINDER Inc for all damages that are a con- sequence of incomplete or incorrect information provided by us, and that we will indemnify and hold harmless BINDER Inc.
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