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Thermo Scientific Cytomat 2 Manual

Thermo Scientific Cytomat 2 Manual

Automatic incubator
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Automatic Incubator Cytomat
2
Software-Documentation
Valid: 02.2007 / 50069763

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Summary of Contents for Thermo Scientific Cytomat 2

  • Page 1 ® Automatic Incubator Cytomat Software-Documentation Valid: 02.2007 / 50069763...
  • Page 2 Enquiries from Germany Phone Sales 0800 1 536376 Service 0800 1 112110 0800 1 112114 Enquiries from other countries Phone + 49 (0) 6184 / 90-6940 + 49 (0) 6184 / 90-7474 www.thermo.com ® Software-Documentation Automatic Incubator Cytomat Thermo Scientific...

  • Page 3: Table Of Contents

    5. Error processing ................37 Checking the process system commands ......... 37 Process control ............... 38 5.2.1 Example: Microplate not stored in the device ......39 5.2.2 Example: MIcroplate not unloaded from the device ....43 Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 4 Positioning of the scanner ............59 Bar code decoding programs ............ 59 Technical data bar code scanner ..........60 9. Annex B, Overview of the registers ..........61 10. Annex C, Overview of the commands ..........64 Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 5: General Notes

    • Keep this software documentation in the vicinity of the device so that safe- ty instructions and important information for checking and adjusting the Plate Shuttle System™ (PSS) are always accessible. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 6: Warranty

    The warranty is valid from the date of delivery of the device to the operator. Explanation of symbols CAUTION! is used if non-observance may cause medium to minor injuries or damage. NOTE is used for applicational hints and useful infor- mation. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 7: Description Of The Software

    • Telegram structure including check sum that can be activated Delivery of the software The software is delivered on CD with the device and can also be ordered se- parately (ID No. 50083010). Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 8: Device Status Queries

    Plate Shuttle System (PSS) [2]. ™ The Plate Shuttle System (PSS) responds immediately with a telegram with the identifier bs and a status byte. ch:bs bs 51 Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 9: Unloading Microplates

    In this example, the error code returned to the process system is 0x32. According to Section 3.2, this code re- presents a transfer station busy error message. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 10: Querying The Status Of The Plate Shuttle™ System (Pss)

    PSS still busy Warning register written Warning No warning Error No error Error register written to Handler Empty Microplate present Automatic lift Closed Open door Device door Closed Open Transfer station Empty Microplate present Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 11 For example, in the high-level command, mv:st ***<CR>, when moving a microtiter plate from the stacker to the transfer station, the Plate Shuttle™ System (PSS) sets the ready bit befor- Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 12: Error Codes

    No automatic lift door configured und wird durch einen Schutzkontaktstecker an einer ordnungsgemäß abgesicherten 0x42 Automatic lift door not open Steckdose angeschlossen. 0x51 Error while accessing internal memory 0x52 Incorrect password / unauthorized access Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 13: Warning Register

    Shovel not retracted 0x09 Initialization due to open device door 0x0C Transfer station did not rotate Query from process system: ch:bw<CR> ™ Response of Plate Shuttle System (PSS): bw **<CR> **Register Warnung in zwei ASCII-Zeichen Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 14: Error Register

    Automatic lift door not closed 0x08 Shovel not retracted 0x0A Stepper motor controller temperature too high 0x0B Communication with heating controllers and CO -supply 0x0C Transfer station not rotated 0xFF Fatal error occurred during error routine Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 15 After resetting the error bit, the Plate Shuttle System (PSS) [2] responds to the command with an ok telegram together with the contents of the overview register in which the error bit was reset before. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 16: Action Register

    Query whether transfer station is in position 2 0x14 Check mitcroplate on shovel 0x15 Check microplate on transfer station 0x16 Movement to barcode reader position 0x17 Test barcode reader position 0x18 Read barcode Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 17 à 0x14 Check Microtiter plate on shovel Movement ™ In this example, the Plate Shuttle System (PSS) handler moves to the stacker and checks whether there is currently a microtiter plate on the shovel. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 18: Swap Station Register

    2 = Plate 2 of SWAP station immediately in front of automatic gate Character 2 0 = No microtiter plate on plate 2 Character 3 1 = Microtiter plate on plate 1, facing the on-site pro- cess system Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 19: Process Commands

    2 with number ### (depending on the configuration of the applied stackers). The illustration shows two stackers with a pitch of 23 and a storage capacity of 21 mic- roplates each. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 20: Complex Commands / High-Level Commands

    • Open automatic lift door and place microplate on transfer station ok **<CR>, mv:wt<CR> • Return to wait position er **<CR> • Close automatic lift door Condition: microplate on handler, transfer station empty Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 21 Stacker, Transfer station, Wait position Exposed shovel position outside device. Interpretation of the command: mv:st 024<CR> Transport microtiter plate from storage location (24) of stacker (s) to transfer station (t). Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 22: Example: Transporting A Microplate Into The Device

    The illustration shows the process sequence of a complex movement, activa- ted by the command mv:st 011. A microplate is to be transported from storage location 11 in the device cham- ber to the transfer station. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 23 Microtiter plate placed on transfer station, access by process system enabled Step 7 Query device status, wait for conclusion of remaining process Step 8 Query errors Step 9 End or start of error routine Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 24: Simple Commands / Low-Level Commands

    When programming low-level commands, monitoring the entire process, mo- nitoring microplates on the handler and at the transfer station, and complex error corrections are to be taken over by the process system. All processes must be carefully planned and tested. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 25: Overview Of Low-Level Commands

    Storage location number as three-digit number in ASCII format Overview register or error code in two ASCII characters ™ If the Plate Shuttle System (PSS) controller accepts the command, it responds with ok **<CR>; otherwise, the response is er **<CR>. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 26: Example: Transporting A Microplate From The Stacker To The Wait Position

    The overview register must be evaluated in order to monitor the Plate Shuttle System (PSS). In particular, the bits for recognizing the microplate on the handler or the transfer station must be checked (refer to flowchart, steps 2 and 21). Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 27 Step 6 Rotate handler in to stacker (location 12) Step 7 Wait for confirmation Step 8 Query device status, wait for process conclusion Step 9 Extend shovel Step 10 Wait for confirmation Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 28 Step 14 Query device status, wait for process conclusion Step 15 Retract shovel Step 16 Wait for query Step 17 Query device status, wait for process conclusion Step 18 Query microtiter plate on handler Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 29 Process commands Process commands Step 19 Move to wait position Step 20 Wait for confirmation Step 21 Query device status, wait for process conclusion Step 22 End or start of external error routine Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 30: Location Scan

    ***<CR> *** location number ™ to query each storage location for occupancy. The Plate Shuttle System (PSS) responds as follows: sc *<CR> *0 location empty *1 location occupied Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 31: Location Scan With Barcode Reader (Optional)

    NOTE – Evaluation When evaluating the location scan, make sure that there is no further access between the test routine and the readout as any such access would make the scan result invalid. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 32: Querying The Most Recently Transported Microtest Plate (High-Level Commands)

    The character string of the response telegram is generally 20 characters long. If the read code is shorter, spaces are output instead of a value. If a barcode is unreadable, the output consists of 1 hyphen and 19 spaces. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 33: Identifying Location Positions (Low-Level Commands)

    ### ll:bc<CR> for one reading of the barcode at the current position The read barcode can then be queried using the command: ch:bc<CR> The PSS responds with the following telegram: bc ####################<CR> Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 34 • Position of the receptacle on the baseplate (1 or 2) • Pitch values in mm (17, 23, 28 etc.) When the command is entered, the leading zeroes must be entered as well. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 35: Communication With Heating System Control And Co Supply

    System (PSS) responds with an error message for the telegram structure: er 03<CR>. If communication with the control board fails, the action is cancelled after a while (busy bit set) with an error message(0x0d). Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 36 ™ Response of Plate Shuttle System (PSS) [2]: tb 24.0 22.3<CR> ™ The Plate Shuttle System (PSS) indicates a set nominal value of 24° C and a current actual value of 22.3° C. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 37: Error Processing

    Transfer station empty 0x32 Transfer station occupied 0x33 Transfer station not in position 0x41 No gate configured 0x42 Automatic lift door not open 0x51 Error while accessing internal memory 0x52 Password incorrect / unauthorized access Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 38: Process Control

    For these errors, the handler returns to the wait position if the error routine was not successful and the automatic gate ™ is closed. The Plate Shuttle System (PSS) is immediately ready for a new com- mand. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 39: Example: Microplate Not Stored In The Device

    System (PSS) confirms the command, the internally programmed process starts immediately. If an error occurs, the error bit in the overview menu is set and the busy bit was reset (Step 6 in the flowchart). Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 40 Error processing Step 1 Send command Step 2 Wait for confirmation Step 3 Evaluate error code Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 41 System (PSS) controller or in a motor controller. Solution: Contact Technical Service. • Error during the error routine: Several errors occurring simultaneously will prevent the error routines from being successfully concluded. Solution: Contact Technical Service. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 42 Error processing Step 4 Query device status Step 5 Read errors from error register Step 6 Microplate retrieved from transfer station? Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 43: Example: Microplate Not Unloaded From The Device

    Readjust as required. • Incorrect shovel position: The handler shovel was not retracted. Solution: Check the microplate (too heavy or jammed), check the handler and stacker position. Readjust as required. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 44 System (PSS) controller or in a motor controller. Solution: Contact Technical Service. • Error during the error routine: Several errors occurring simultaneously will prevent the error routines from being successfully concluded. Solution: Contact Technical Service. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 45: Communication With Telegram Structure

    System (PSS) could respond as follows: < STX | o | k | 0 | 1 | ; |BCC| ETX > < STX |0x6f|0x6b|0x20|0x30|0x31|0x3B|0x25| ETX > or, converted to the familiar form: ok 01<CR> Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 46: Description Of The Service Program

    Pentium 1 GHz or higher • Windows 2000 SP3, Windows XP • Min. 128 MB RAM, 256 MB recommended • 200 MB free disk space • VGA 1024 * 800 pixel resolution or higher Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 47: Overview Of Menus And Submenus

    Activation of the continuous operation of the Plate Shuttle System (PSS). Init motors + system Initialization of the individual stepper motors. Restart of the motor con- ™ trollers and of the complete Plate Shuttle System (PSS). Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 48 Please observe the following software version interconnections: ™ Software version of the Plate Shuttle System (PSS) ID No.: 50061482 Service program ID No.: 50083010 Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 49: Motor Parameter [Parameter Menu]

    Save for error detection by sending the para- meter settings used for operation to the Tech- nical Service so that setting errors can be corrected and you may receive a functional data record. Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 50: Basic Parameter [Parameter Menu]

    Data can be read from the device. Write: Disabled (can only be activated by service personnel with password). Load: Data can be loaded from a file. Save: Data can be saved to a file. Close: Return to overview window. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 51: Free Running [Testing Menu]

    6 Plain text for current error messages 7 Modifiable input fields, output fields, selection and start button for test rou- tine ™ 8 Running time for complete movement of Plate Shuttle System (PSS) 9 Software version number Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 52 When the test routine is to be run without a microplate, you can use the Plate Identification function to disable microplate identification. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 53: Init Motors + System [Init Menu]

    Init all motors Initialization of all motors Init automatic part Initialization of the automatic unit (automatic lift door, transfer station / handler, lift) ™ Init complete electronics Restart of the Plate Shuttle System (PSS) Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 54: Adjustment Transfer Station [Adjustment Menu]

    Number of steps moving out Use the Read parameter button to read the set parameter values or the Write ™ parameter button to save the set parameter values in the Plate Shuttle stem (PSS). Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 55 Once the adjustment has been completed, all parameters can be saved in ™ the Plate Shuttle System (PSS) by activating the Write parameter button. Use the Read parameter button to read the current parameters of the Pla- ™ te Shuttle System (PSS). Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 56: Stacker Pitch Configuration [Adjustment Menu]

    [6] to the uppermost storage location num- ber in the second stacker [7]. Only after all parameters have been set, the in- put is read and saved by actuating the Write button [5]. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 57: Read Error [Error Menu]

    7.10 password [Password menu] Program functions that are disabled by access protections can be enabled by entering the correct password (usually reserved for Technical Service person- nel). Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 58: Logfile On/Off [Logfile Menu]

    To reset the connection to the original state, perform a power reset. 7.13 open to HERAcell [Communication menu] Establishment of a permanent connection to the heating system control and CO supply (HERAcell). To reset the connection to the original state, perform a power reset. Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 59: Annex A , Bar Code Scanner (Optional)

    System (PSS) must be switched to service mode: sc:c1<CR>. Then, the Keyence software is configured for the interface. Transmission settings for the Keyence software: Speed: 9600 Baud Data bits: Stop bits: Parity: Even Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 60: Technical Data Bar Code Scanner

    Seriell asynchron Transfer parameters 9600 baud, even parity, 7 databit, 1 stopbit Enclosure IP 65 Laser protection class FDA: class II, DIN/IEC: class 2 Humidity 35 %...85 %, non-condensing Temperature 0 °C...50 °C Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...

  • Page 61: Annex B, Overview Of The Registers

    Stepper motor controller temperature too high 0x0B Other stepper motor controller error 0x0C Transfer station not rotated 0x0D Communication with heating system control and CO2 supply 0xFF Fatal error occurred during error routine Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 62 Test microplate on shovel 0x15 Test microplate on transfer station Value Bit 5-7 Movement target 0x01 Movement target: Init position 0x02 Movement target: Wait position 0x03 Movement target: Stacker 0x04 Movement target: Transfer station Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 63 Transfer station occupied 0x33 Transfer station not in position 0x41 Automatic lift door not configured 0x42 Automatic lift door not open 0x51 Error while accessing internal memory 0x52 Incorrect password / unauthorized access Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...

  • Page 64: Annex C, Overview Of The Commands

    • Close automatic lift door Condition: microplate at transfer station, handler empty • Return to wait position from exposed position outside device ok **<CR>, mv:wh<CR> • Stop at position above transfer station er **<CR> Condition: None Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 65 • Read barcode at current position ll:bc er **<CR> Condition: none ok **<CR>, • Move motors to wait position ll:wp<CR> er **<CR> Condition: None ok **<CR>, • Reinitialize automatic unit ll:in<CR> er **<CR> Condition: None Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 66 **<CR> Condition: Value must be within device control value range ok **<CR>, • Input nominal value for CO content ll:ic XX.X<CR> er **<CR> Condition: Value must be within device control value range Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 67 Thermo Scientific Software-Documentation Automatic Incubator Cytomat ®...
  • Page 68 Software-Documentation Automatic Incubator Cytomat ® Thermo Scientific...
  • Page 69 Internet: http://www.thermo.com ® Thermo Scientific Software-Documentation Automatic Incubator Cytomat...

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