Page 1 Thermo Scientific HERA 160i/ CELL VIOS HERA 250i CELL VIOS Incubator User Manual 50144132 A July 31, 2015...
Page 2 2015 Thermo Fisher Scientific Inc. All rights reserved. Trademarks HERACELL VIOS™, Steri-run™, Steri-cycle™, iCan™ and THRIVE™ are registered trademarks of Thermo Scientific. Thermo Scientific is a brand of Thermo Fisher Scientific Inc. All other trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers.
Page 3: Table Of Contents
Packaging ............................1-1 Acceptance inspection......................... 1-1 Standard equipment components HERACELL VIOS 160i / 250i............1-2 Optional equipment components HERACELL VIOS 160i / 250i ............. 1-2 Optional equipment components HERACELL VIOS 160i..............1-2 Optional equipment components HERACELL VIOS 250i..............1-3 Chapter 2 Installation of the device ....................2-1 Environmental Conditions .........................
Page 4 HERACELL VIOS 160i / VIOS 250i Front view ................3-2 HERACELL VIOS 160i / VIOS 250i Rear view ................3-4 Looped-through gas supply at HERACELL VIOS 160i / HERACELL VIOS 250i: ...... 3-5 Safety devices HERACELL VIOS 160i / VIOS 250i................3-6 Workspace atmosphere ........................
Page 5 Chapter 8 Cleaning and disinfection....................8-1 Cleaning, ............................8-1 Decontamination procedures ......................8-2 Preparing for disinfection or steri-run ....................8-2 Wipe/spray disinfection ........................8-3 Steri-run decontamination routine...................... 8-7 Activating steri-run ........................8-9 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 6 Replacing the Door Seal ........................9-11 Chapter 10 Waste disposal ....................... 10-1 Overview of the materials used: ......................10-1 Chapter 11 Specifications ......................... 11-1 HERACELL VIOS 160i ........................11-2 HERACELL VIOS 160i ........................11-4 HERACELL VIOS 250i ........................11-6 HERACELL VIOS 250i ........................11-8 Chapter 12 Annex: Data communication..................
Page 7 Table of Contents Program HERACELL VIOS 160i / HERACELL VIOS 250i............12-21 Installing the HERACELL VIOS 160i & HERACELL VIOS 250i ........... 12-22 Operating the HERACELL VIOS 160i & HERACELL VIOS 250i ......... 12-23 Chapter 13 Device log ........................13-1 Chapter 14 Contact Information Thermo Scientific ................
Page 8 Table of Contents 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 9 Figure 3-1. HERA CELL VIOS 160i / VIOS 250i Front view..............3-2 Figure 3-2. HERACELL VIOS 160i / VIOS 250i Rear view ..............3-4 Figure 3-3. Looped-through gas supply HERACELL VIOS 160i / HERACELL VIOS 250i ....3-5 Figure 3-4. HEPA-filter and airbox ......................3-8 Figure 3-5.
Page 10 Figure 6-29. Choose the desired reminder interval function..............6-26 Figure 6-30. Setting the reminder interval for steri-run .................6-27 Figure 6-31. Event logging menu ......................6-28 Figure 6-32. Event display........................6-28 Figure 6-33. Logging cycle setting ......................6-29 Figure 6-34. Displaying the error table....................6-31 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 11 Figure 9-4. Gas-tight screen measurement opening ................9-6 Figure 9-5. CO2 calibration procedure....................9-7 Figure 9-6. Removing the airbox ......................9-8 Figure 9-7. Installing the HEPA-filter ....................9-9 Figure 9-8. Gas inlet filter installation ....................9-10 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 12 Figures 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 13: Preface
• which protective measures apply when specific agents are used, • which measures are to be taken in the case of an accident. • Repairs to the device must be carried out only by trained and authorized expert personnel. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 14: Applicability Of The Instructions
Thermo Scientific immediately for your own safety. Warranty Requirements for warranty Thermo Scientific warrants the operational safety and functions of the CO incubator only under the condition that: • the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions, •...
Page 15: Explanation Of Safety Information And Symbols
CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or moderate injuries. CAUTION Indicates a situation which, if not avoided, could result in property damage. Note Is used for application al hints and useful information. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 16: Additional Symbols For Safety Informations
Preface Explanation of safety information and symbols Additional symbols for safety informations: Wear safety gloves! Wear safety goggles! Harmful liquids! Electric shock! Hot surfaces! Fire hazard! Explosion hazard! Suffocation hazard! 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 17: Symbols On The Device
To that end, a controlled set of physiological ambient conditions is produced in the workspace of the device by exerting precise control of the following parameters: • Temperature • CO content • O content • Relative humidity 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 18: Incorrect Use
• FCC 47, Part 15, § 15.107, Measurement 2015, EMC regulation FCC • FCC 47. Part 15, § 15.109, Measurement 2015, EMC regulation FCC For other countries, the applicable national regulations are binding. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 19: Safety Notes On Gases
) released into the room atmosphere may cause suffocation. If CO is released, initiate safety measures immediately! • Leave the room immediately and do not allow others to enter the room! • Inform security service or fire department! 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 20: Safety Notes On Oxygen (O2)
) released into the room atmosphere may cause suffocation. If N released, initiate safety measures immediately! • Leave the room immediately and do not allow others to enter the room! • Inform security service or fire department! 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 21: Chapter 1 Delivery Of The Incubator
• possible damage. If components are missing or damage is found on the device or the packaging, esp. damages caused by humidity and water, please contact the linehauler as well as the Technical Support of Thermo Scientific immediately. 160i / 250i...
Page 22: Standard Equipment Components Heracell Vios 160I / 250I
Delivery of the Incubator Standard equipment components HERACELL VIOS 160i / 250i Standard equipment components HERA 160i / 250i CELL VIOS Quantity of components supplied Pieces Water reservoir cover panel Max. water level sensor Rear wall air duct Airduct top...
Page 23: Optional Equipment Components Heracell Vios 250I
Delivery of the Incubator Optional equipment components HERACELL VIOS 250i Optional equipment components HERA 250i CELL VIOS Components delivered with 250 liter units with split inserts Pieces Support brackets Split inserts Sixfold gas-tight screen 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 24 Delivery of the Incubator Optional equipment components HERACELL VIOS 250i 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 25: Chapter 2 Installation Of The Device
• Relative humidity up to 80% (max.). • No direct exposure to sunlight. • Devices that produce excessive heat must not be placed near the location of HERA 160i / CELL VIOS HERA 250i CELL VIOS 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 26: Room Ventilation
VIOS 250i VIOS 160i All dimensions in mm. Figure 2-1. Device dimensions Note Accessibility of the devices: To ensure the accessibility for care and maintenance works, keep larger side and rear distances. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 27: Transport
2-3) is available in order for the units to be movable. The rollers of the base rack are fitted with a locking brake. Note when installing the stacking adapter and stacking the units, follow the assembly instructions delivered with the stacking adapter. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 28: Figure 2-3. Stacking
Installation of the device Stacking Note Moving stacked devices: Stacked devices provided with a mobile base rack must only be moved inside rooms on even floors without any slopes. Figure 2-3. Stacking 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 29: Figure 2-4. Stacking Adapter And Base Rack With Stacking Elements
2-5) to the opposite corner of the unit using two screws. 6. Place the device to be stacked with the device stands (6/Figure 2-5) onto the stacking elements (1/Figure 2-4) at the top of the adapter plate. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 30: Retrofitting/Modifications
(replaces the standard TCD sensor), • 4-20 mA data interface. • gas cylinder monitor Note Modifications: Retrofittings and modifications must only be performed by the Technical Service of Thermo Electron LED GmbH. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 31: Chapter 3 Description Of The Device
Description of the device Table of Contents • “HERACELL VIOS 160i / VIOS 250i Front view“ page 3-2 • “HERACELL VIOS 160i / VIOS 250i Rear view“ page 3-4 • “Safety devices HERACELL VIOS 160i / VIOS 250i“ page 3-6 •...
Page 32 Description of the device HERACELL VIOS 160i / VIOS 250i Front view HERA 160i / 250i Front view CELL VIOS VIOS 14a,b Figure 3-1. HERA 160i 250i Front view CELL VIOS / VIOS 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 33 Description of the device HERACELL VIOS 160i / VIOS 250i Front view 1. Pressure compensation opening /Access port with plug 2. Door switch for glass door 3. Temperature Sensor 4. O sensor (optional) 5. Door handle for the glass door or the gas-tight screen 6.
Page 34 Description of the device HERACELL VIOS 160i / VIOS 250i Rear view HERA 160i 250i Rear view CELL VIOS / VIOS Figure 3-2. HERA 160i 250i Rear view CELL VIOS / VIOS 1. Control box with supply interface for combined gas connection (optional) CO...
Page 35 Description of the device HERACELL VIOS 160i / VIOS 250i Rear view 11. Distributor connection for looping through the CO gas supply for up to three additional devices (independent of device type) Hidden: Distributor connection for looping through the O...
Page 36: Safety Devices Heracell Vios 160I / Vios 250I
Description of the device Safety devices HERACELL VIOS 160i / VIOS 250i 5. Gas pressure hose for looping-through the gas supply 6. Diagram: CO and O gas connection with optional CO and O gas monitoring 7. HERA 160i / HERA 250i with looped-through gas supply;...
Page 37: Relative Humidity
The pre-filter must remain installed in the device during the Steri-run decontamination routine, but needs to be removed for refilling the water reservoir. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 38: Hepa-Filter And Air Duct
3-5) to the workspace ceiling, thus ensuring an optimum temperature distribution. At the same time, it directs the incoming stream of process gases into workspace and ensures that the gases are optimally intermixed. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 39: Co2 Supply
21 % to 90 %. Operation with higher oxygen concentrations requires that the fire protection caution notes in Preface in the section Safety notes on gases on page be heeded. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 40: N2 Supply
The outer door can only be closed after the glass door has been latched properly. Note Versions with gas-tight screen: For devices with the optional gas-tight screen, the door switch function described above is activated when the outer door is opened. 160i / 250i HERA 3-10 CELL VIOS Thermo Scientific...
Page 41: Sensor System
3-8) needs to be refilled. When the water reservoir level falls to 0,5 l, the display field rH shows Error - rH - No water (see also “Error messages“ page 6-45). 160i / 250i HERA Thermo Scientific CELL VIOS 3-11...
Page 42: Figure 3-8. Sensors For Water Level
• the alarm relay responds. If the error message is accepted, the display shows the Overtemperature icon to indicate the response of the thermal protection, and the temperature display is highlighted in red. 160i / 250i HERA 3-12 CELL VIOS Thermo Scientific...
Page 43: Supply Interface
Description of the device Supply interface Supply interface Standard interfaces Figure 3-9. Interfaces (right side of control box) 160i / 250i HERA Thermo Scientific CELL VIOS 3-13...
Page 44: Optional Interfaces
2. 4...20 mA interface (option) 3. O connector (option, only available with CO - and O - gas supply sources and simultaneous use of gas monitoring option) 160i / 250i HERA 3-14 CELL VIOS Thermo Scientific...
Page 45: Gas Connection
D/A converter. The D/A converter provides a resolution of 16 bit per channel, equivalent to 65536 incremental values. The interface port accepts various customer-supplied external measurement devices with 4-20 mA inputs - for example, the Thermo Scientific Smart-Vue Wireless Monitoring System (not supplied). The table below lists the output signals: Channel...
Page 46: Alarm Contact
Mains power is connected to the unit using a cable with an IEC connector plugged into the IEC socket in the control box (7/Figure 3-9). The mains socket must be easily identifiable by the user and freely accessible at all times. 160i / 250i HERA 3-16 CELL VIOS Thermo Scientific...
Page 47: Workspace Components
Glass door and optional gas-tight screen The standard version of the HERA 160i and HERA 250i units is equipped with a CELL VIOS CELL VIOS single-locked door made of tempered safety glass. 160i / 250i HERA Thermo Scientific CELL VIOS 3-17...
Page 48: Figure 3-11. Heracell Vios 160I With Glass Door
• relative humidity. Gas-tight screens are available as options for both types of products: • HERA 160i: threefold gas-tight screen (see Figure 3-12), CELL VIOS • HERA 250i: sixfold gas-tight screen. CELL VIOS 160i / 250i HERA 3-18 CELL VIOS Thermo Scientific...
Page 49: Water Reservoir
The pre-filter must remain installed in the device during the Steri-run decontamination routine, but needs to be removed for refilling the water reservoir. 160i / 250i HERA Thermo Scientific CELL VIOS 3-19...
Page 50: Figure 3-13. Water Reservoir
Plugging the drain tube supplied into the front-side quick-drain valve starts draining immediately. Figure 3-14. Drain valve of water reservoir 160i / 250i HERA 3-20 CELL VIOS Thermo Scientific...
Page 51: Heating System
The pressure compensation opening (3/Figure 3-15) located directly beneath the access port in the rear wall of the unit ensures pressure compensation between the workspace of the unit and the operating room. 160i / 250i HERA Thermo Scientific CELL VIOS 3-21...
Page 52: Shelf System
Figure 3-16, 160i with a cutout for refilling (4/Figure 3-16). For details on using the shelf system, see HERA CELL VIOS “Start-up“ page 4-1. 160i / 250i HERA 3-22 CELL VIOS Thermo Scientific...
Page 53: Chapter 4 Start-Up
Before the initial start-up, the device must be decontaminated. Before the decontamination is performed, the following workspace components must be cleaned: • Support rail • Shelf support 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 54: Installing The "Max" Fill Level Indicator And The Pre-Filter
3. Suspend the “MAX” fill level indicator (1/Figure 4-1) in the slot provided in the water reservoir cover panel (5/Figure 4-1). Figure 4-1. “MAX” fill level indicator 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 55: Figure 4-2. „Max" Fill Level Indicator And Pre-Filter
Start-up Installing the “MAX” fill level indicator and the pre-filter 4. Insert the pre-filter (4/Figure 4-2) into the water reservoir cover panel. 1, 2 Figure 4-2. „MAX“ fill level indicator and pre-filter 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 56: Install The Air Duct
4-3) on the two rear wall studs and tilt the air duct backwards. 3. Lock the lateral keyholes of the top part (step G/Figure 4-3) into the retaining screws in the workspace ceiling. Figure 4-3. Assembling the air duct 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 57: Installing The Hepa-Filter And The Water Reservoir Cover Panel
4-4) on the right side of the airbox into the HEPA-filter catches (6/Figure 4-4). 4. Place the seal (8/Figure 4-4) into the groove (7/Figure 4-4) provided at the airbox tubing and press it down evenly. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 58: Figure 4-5. Place The Airbox Onto The Water Reservoir Cover Panel
9. Let the front edge of the water reservoir cover slide into the water reservoir (2/Figure 4-6). The airbox tubing slides into the fan outlet. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 59: Installing The Shelf System
2. Clamp the lockspring behind the upper embossing. 3. To remove the support rails, pull the lockspring tab down out of the embossing and remove the rail. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 60: Shelf Support Installation
160i incubator be sure to install the insert with the cut-out for CELL VIOS refilling at the bottom position only. Levelling the device 1. Position a bubble level onto the center shelf or onto the roller holder. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 61: Mount The Split Inserts (Optional) Into The Heracell Vios 250I
1. Place both these shelves (1/Figure 4-9) onto the support frame (2/Figure 4-9) (step A). 2. Suspend the support frame (2/Figure 4-9) in the shelf supports (3/Figure 4-9) (step B). 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 62: Gas Connection
4-10) from the sterile filter. 3. Slide the hose clamp (1/Figure 4-10) onto the gas pressure hose (2/Figure 4-10) and connect hose to the sleeve (4/Figure 4-10) of the sterile filter (5/Figure 4-10). 160i / 250i HERA 4-10 CELL VIOS Thermo Scientific...
Page 63: Gas Connection Without Gas Monitoring System
The pipe of the pressure compensation opening must not be extended or redirected. Gas connection without gas monitoring system Figure 4-11. Gas connection without gas monitoring system 160i / 250i HERA Thermo Scientific CELL VIOS 4-11...
Page 64: Co2 Connection With Gas Monitoring System (Optional)
On devices without the optional gas monitoring system, there is no automatic gas monitoring. Therefore, the filling level of the gas bottles must be checked every day. connection with gas monitoring system (optional) Figure 4-12. CO connection with gas monitoring system (optional) 160i / 250i HERA 4-12 CELL VIOS Thermo Scientific...
Page 65 By looping the gas supply through, up to 4 devices can be supplied by a CO gas supply. Combined CO and O connection with gas monitoring system (optional) Figure 4-13. Combined CO connection with gas monitoring system (optional) 160i / 250i HERA Thermo Scientific CELL VIOS 4-13...
Page 66 Note Looping the gas supply through: By looping the gas supply through, up to 4 devices (independent of device type) can be supplied by a CO gas supply or a O gas supply. 160i / 250i HERA 4-14 CELL VIOS Thermo Scientific...
Page 67: Power Supply Connection
5. Mains connection: The mains socket must be easily identifiable by the user and freely accessible at all times. The power cord plug is the all-pole disconnecting device from the mains supply. 160i / 250i HERA Thermo Scientific CELL VIOS 4-15...
Page 68: Connecting The Usb Interface
Connecting the alarm contact: Note Skilled work: Thermo Scientific warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately...
Page 69: Figure 4-15. Connection Example Alarm Contact
1. Wire the individual conductors as shown in the wiring diagram. 2. Connect the connector of the alarm system connecting cable to the interface at the control box at the rear panel of the device. 160i / 250i HERA Thermo Scientific CELL VIOS 4-17...
Page 70: Connecting The 4-20 Ma Interface
Figure 4-16. Pin-out of the 4-20 mA interface Note Skilled work: Thermo Scientific warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately...
Page 71: Chapter 5 Operation
• The airbox with the HEPA-filter and the air duct must be installed according to the instructions. Decontaminating the device workspace: • Run the steri-run decontamination routine (see “Steri-run decontamination routine“ page 8-7) or decontaminate the workspace according to the hygiene regulations set forth by the operator. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 72 When the filling level drops below a minimum value, it must be possible to top up water during the work process. Fill volume for HERA 160i and HERA 250i: 3 l CELL VIOS CELL VIOS 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 73: Starting Operation
250i ): Remove bottom insert if there is not enough space for CELL VIOS the container used for filling. 4. Fill reservoir with sufficiently purified water (beneath cover item 1 in Figure 5-1) of the workspace. Figure 5-1. Water reservoir 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 74: Figure 5-2. "Max" Fill Level Indicator
16. The CO control supplies the set amount of CO 17. If the auto-start routine has been completed, the progress indicator is hidden, and the main menu is displayed. The device is operational. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 75 70% max. Bulky objects in the workspace that dissipate heat may impair heat distribution. Bulky objects in the use able space that dissipate heat may impair heat distribution. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 76 Operation Starting operation 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 77: Chapter 6 Handling And Control
“auto-start function“ page 6-12 • “Launching steri-run“ page 6-16 • “User configuration“ page 6-17 • “Trend display scaling“ page 6-43 • “Error messages“ page 6-45 • “Measures after power outage“ page 6-47 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 78: Power Switch
• After a short beep the blank screen vanishes and the touchscreen is displayed. • The control loop sensors pass the heat-up phase (“Heat-up phase of the control loop sensors“ page 6-7). • Powering down: Press the power switch; the switch illumination goes off. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 79: Operating Panel And Operating Structure
“Icon description“ page 6-40), • steri-run key, • Menu key, • auto-start key. Note Extended pressure-sensitive area: To accept a failure message, the entire touchscreen can be used as a pressure-sensitive area. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 80: Versions Without O2/N2 Control
“Icon description“ page 6-40) 4. Key for starting the steri-run decontamination routine 5. Key for opening the menu navigation 6. Key for starting the auto-start routine 7. Display field water level RH 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 81: Version With Combined Co2/O2/N2 Control (Optional)
• A: Direct access to the control loop settings: Temperature, CO -setpoint, • B: Starting the Steri-Run or auto-start routine of the unit, • C: Navigation through the submenus for device configuration. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 82: Figure 6-5. Menu Structure Overview
Handling and control Operating panel and operating structure Figure 6-5. Menu structure overview 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 83: Factory Presettings Of The Ican™ Touchscreen Controls
The start process is indicated by an audible signal. During the heat-up phase, the displays show dots (...) instead of values: • Temperature display, • CO display and • O display field 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 84: Behavior Of Keys During Adjustments
Figure 6-7. Temperature display field and temperature selection menu 2. To exit the Temperature menu: • Press the End key [3]. 3. To set the temperature set value: • Press the Set value key. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 85: Setting The Co2 Set Value
1. Press the CO display key. • The CO menu is displayed. Figure 6-9. CO display and CO menu 2. To exit the CO menu: • Press the End key [3]. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 86: Setting The O2 Set Value
Setting the O set value This setting is possible only on versions with the optional O control. 1. Press the O display key. • The O menu is displayed. 160i / 250i HERA 6-10 CELL VIOS Thermo Scientific...
Page 87: Figure 6-11. O2 Display And O2 Menu
• Press the Enter key. • The system returns to the main menu. The O display shows the actual value currently measured in the workspace. Figure 6-12. Setting the O set value 160i / 250i HERA Thermo Scientific CELL VIOS 6-11...
Page 88: Auto-Start Function
When temperature and relative humidity have reached constant values, the measuring system is automatically adjusted to these values, and the workspace is supplied with the preset quantity of CO 160i / 250i HERA 6-12 CELL VIOS Thermo Scientific...
Page 89 • calibration values too high or too low, • No communication with sensor. Gas supply CO control loop: • No communication with sensor. In this case, the auto-start key is dimmed and its function is not available. 160i / 250i HERA Thermo Scientific CELL VIOS 6-13...
Page 90: Activating The Auto-Start Routine
3. Refill water, if necessary. 4. Activate the auto-start routine: • Press the Start key. 5. To air the workspace, open both device doors. When the audible alarm sounds after 30 seconds, 160i / 250i HERA 6-14 CELL VIOS Thermo Scientific...
Page 91: Interrupting The Auto-Start Routine
• The system returns to the status display, and the auto-start routine is resumed. 2. To cancel the auto-start routine: • Press the End key [3]. • The warning triangle is displayed as a failure message together with the audible signal. 160i / 250i HERA Thermo Scientific CELL VIOS 6-15...
Page 92: Launching Steri-Run
• The system returns to the main menu. Launching steri-run The steri-run decontamination routine is used to decontaminate the complete workspace. An in-depth description of the function is given in the chapter Cleaning and disinfection (Chapter 8‚ page 160i / 250i HERA 6-16 CELL VIOS Thermo Scientific...
Page 93: User Configuration
• Changing the keypad lock code, • Date/time setting, • Adjusting display brightness, • Key tone setting, • Interface configuration, • User display screen language setting, • Reminder interval setting. 160i / 250i HERA Thermo Scientific CELL VIOS 6-17...
Page 94: Figure 6-18. Settings/Setup Menu
Figure 6-19. Changing the keypad lock code 3. To enter the default 0000: • Press the corresponding numeric keys. • The number combination is displayed as encrypted text in the input box. 160i / 250i HERA 6-18 CELL VIOS Thermo Scientific...
Page 95: Figure 6-20. Changing The Keypad Lock Code
The input dialog allows date and time to be set to the required time zone. 1. Press the Menu key. 2. Select the menu command Date / Time. • The selection dialog shown in Figure 6-21 is displayed. 160i / 250i HERA Thermo Scientific CELL VIOS 6-19...
Page 96: Figure 6-21. Date/Time Menu
• Press the Enter key. 7. The system returns to the Date/Time menu. 8. For changing the time, choose the Time option. • The input dialog shown in Figure 6-23 is displayed. 160i / 250i HERA 6-20 CELL VIOS Thermo Scientific...
Page 97: Figure 6-23. Setting The Time
Setting the contrast 1. Press the Menu key. 2. Select the menu command Settings / Setup. 3. Select the option Display contrast. • The input dialog shown in Figure 6-24 is displayed. 160i / 250i HERA Thermo Scientific CELL VIOS 6-21...
Page 98: Figure 6-24. Adjusting Display Brightness
Adjusting key tone volume 1. Press the Menu key. 2. Select the menu command Settings / Setup. 3. Select the option Key tones. • The input dialog shown in Figure 6-25 is displayed. 160i / 250i HERA 6-22 CELL VIOS Thermo Scientific...
Page 99: Figure 6-25. Adjusting Key Tone Volume
The baud rate can be changed within the defined baud rates (9,600, 19,200, 38,400, 57,600 baud). Setting the baud rate 1. Press the Menu key. 2. Select the menu command Settings / Setup. • The selection dialog shown in Figure 6-26 is displayed. 160i / 250i HERA Thermo Scientific CELL VIOS 6-23...
Page 100: Figure 6-26. Interface Usb Menu
7. To activate the new settings: • Browse back to the main menu. • Wait for approx. 10 seconds and perform a reset by switching the device off and on using the power switch. 160i / 250i HERA 6-24 CELL VIOS Thermo Scientific...
Page 101: Figure 6-28. Language Setting
5. The new language appears in the display. The New message indicates that the selection has been changed, but not saved yet. 6. To accept and save the selection: • Press the Enter key. • The system returns to the Settings/Setup menu. 160i / 250i HERA Thermo Scientific CELL VIOS 6-25...
Page 102: Figure 6-29. Choose The Desired Reminder Interval Function
Figure 6-29 is displayed. Figure 6-29. Choose the desired reminder interval function. 3. Select the appropriate menu option, for example Steri-run. • The input dialog shown in Figure 6-29 is displayed. 160i / 250i HERA 6-26 CELL VIOS Thermo Scientific...
Page 103: Data Logging
The Event logging selection menu (Figure 6-31) allows for accessing dialog screens for logging and displaying events that occur during ongoing operation of the unit: • Event display, • Recording interval, • Error table. 160i / 250i HERA Thermo Scientific CELL VIOS 6-27...
Page 104: Figure 6-31. Event Logging Menu
• The selection dialog shown in Figure 6-31 is displayed. 3. Select the menu command Event display. • The list shown in Figure 6-32 appears on the display. Figure 6-32. Event display 160i / 250i HERA 6-28 CELL VIOS Thermo Scientific...
Page 105: Figure 6-33. Logging Cycle Setting
• The selection dialog shown in Figure 6-31 is displayed. 3. Select the menu command Recording interval. • The input dialog shown in Figure 6-33 is displayed. Figure 6-33. Logging cycle setting 160i / 250i HERA Thermo Scientific CELL VIOS 6-29...
Page 106 2. Select the menu command Event logging. • The selection dialog shown in Figure 6-31 is displayed. 3. Select the menu command Event display. • The list shown in Figure 6-34 appears on the display. 160i / 250i HERA 6-30 CELL VIOS Thermo Scientific...
Page 107: Options
6-35) allows for accessing all selection dialogs for the functional options of the unit: • Alarm, • Low humidity, • Gas-tight screen (optional), • Humidity sensor, • Audible alarm, • O gas supply (optional), • HEPA-filter. 160i / 250i HERA Thermo Scientific CELL VIOS 6-31...
Page 108: Figure 6-35. Options Menu
• The selection dialog shown in Figure 6-36 is displayed. Figure 6-36. Alarm menu 4. Select the menu command Alarm relay. • The input dialog shown in Figure 6-37 is displayed. 160i / 250i HERA 6-32 CELL VIOS Thermo Scientific...
Page 109: Figure 6-37. Alarm Relay Setting
2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. 3. Select the menu command Low humidity. • The input dialog shown in Figure 6-38 is displayed. 160i / 250i HERA Thermo Scientific CELL VIOS 6-33...
Page 110: Figure 6-38. Low Humidity Setting
Failure to set the gas-tight screen function in accordance with the actually installed door version may impair the incubation performance. Gas tight screen setting 1. Press the Menu key. 2. Select the menu command Options. 160i / 250i HERA 6-34 CELL VIOS Thermo Scientific...
Page 111: Figure 6-39. Gas Tight Screen Setting
2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. 3. Select the menu command Water level sensor. • The input dialog shown in Figure 6-40 is displayed. 160i / 250i HERA Thermo Scientific CELL VIOS 6-35...
Page 112: Figure 6-40. Setting The Water Level Sensor
2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. 3. Select the menu command Buzzer. • The input dialog shown in Figure 6-41 is displayed. 160i / 250i HERA 6-36 CELL VIOS Thermo Scientific...
Page 113: Figure 6-41. Alarm Relay Setting
2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. 3. Select the menu command O • The input dialog shown in Figure 6-42 is displayed. 160i / 250i HERA Thermo Scientific CELL VIOS 6-37...
Page 114: Figure 6-42. Switching The O2 Control On And Off
The gas monitoring system remains active even when the O control is switched off or the -sensor is disabled. 160i / 250i HERA 6-38 CELL VIOS Thermo Scientific...
Page 115: Figure 6-43. Hepa Configuration
4. Select the menu command HEPA configuration. • The input dialog shown in Figure 6-44 is displayed. Figure 6-44. Activating / de-activating the HEPA-filter 1. To toggle between two states: • Press the + key. 160i / 250i HERA Thermo Scientific CELL VIOS 6-39...
Page 116: Icon Description
Error message, indicating, that the filling level of one or of several gas cylinders is too low to ensure regular gas supply. The monitoring function is available only for device versions with the optional gas monitoring system. 160i / 250i HERA 6-40 CELL VIOS Thermo Scientific...
Page 117: Figure 6-46. Gas Monitoring Icons
(full / empty). The icon with the blue frame indicates the cylinder not in use that will be switched over to continue the supply. Figure 6-46. Gas monitoring icons • Manual changeover to a full gas cylinder is possible. 160i / 250i HERA Thermo Scientific CELL VIOS 6-41...
Page 118: Enabling/Disabling The Keypad Lock
6-18). Resetting the code: If the keypad lock code is no longer available, the code must be reset to the standard code by the Technical Support of Thermo Fisher Scientific. 160i / 250i HERA 6-42 CELL VIOS Thermo Scientific...
Page 119: Software Versions
The trend display of the three control loops: • temperature, • 0...20% CO • 0...100% O can be scaled to two different versions. 1. Full screen display • Press the CO display field in the main menu. 160i / 250i HERA Thermo Scientific CELL VIOS 6-43...
Page 120: Figure 6-49. Invoking The Co2 Concentration Graph Display
• During zoom mode the trend display can be scrolled forward and back. 4. To return to the total trend: • Open rectangle over a small diagram section and press any position outside the marked area. 160i / 250i HERA 6-44 CELL VIOS Thermo Scientific...
Page 121: Error Messages
• The Error dialog box appears and the detected error is displayed, • the audible alarm is switched off. 2. To exit the error display: • Press the End key [3]. • The error message disappears. 160i / 250i HERA Thermo Scientific CELL VIOS 6-45...
Page 122: Resetting Overtemperature Protection
1. Displaying the cause of an error: • Press anywhere on the touchscreen. • The Error dialog box appears and the detected error is displayed. • The audible alarm is switched off. 160i / 250i HERA 6-46 CELL VIOS Thermo Scientific...
Page 123: Measures After Power Outage
Alternatively, or when the above mentioned measures were not effective, start a disinfection run at 180 °C. Refer to Chapter “Steri-run decontamination routine“ page 8-7. The disinfection routine can be interrupted after appro. 1 hour. The sensors should then have dried. 160i / 250i HERA Thermo Scientific CELL VIOS 6-47...
Page 124: Troubleshooting
Error: Fan Actual value of the fan Reset unit. If the error is out of tolerance. occurs repeatedly, call service. IR sensor changed New serial number Acknowledge the detected alarm 160i / 250i HERA 6-48 CELL VIOS Thermo Scientific...
Page 125 Reset unit. If the plausible temperature signal error occurs repeatedly, call service. Calibration values too Max. adjust value for Acknowledge alarm, high/low temperature exceeded / enter different target not attained value. 160i / 250i HERA Thermo Scientific CELL VIOS 6-49...
Page 126 Chapter “Measures after power outage“ page 6-47. Cylinder A empty Gas cylinder A is empty Replace gas cylinder A Cylinder B empty Gas cylinder B is empty Replace gas cylinder B 160i / 250i HERA 6-50 CELL VIOS Thermo Scientific...
Page 127 - 45 min. after a door was opened, - 159 min. after a setpoint change. This setting can be changed by field service. 160i / 250i HERA Thermo Scientific CELL VIOS 6-51...
Page 128 Handling and control Measures after power outage 160i / 250i HERA 6-52 CELL VIOS Thermo Scientific...
Page 129: Chapter 7 Shut-Down
Figure 7-1. Drain valve of water reservoir 5. Allow the water reservoir to drain completely into the collecting vessel. 6. Remove drain hose valve again. 7. Start the Steri-Run decontamination routine („Launching steri-run“ page 6-16). 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 130 11. Disconnect the gas pressure hoses from the sleeve at the rear of the device. 12. Until the device is shut down, the workspace must be continuously ventilated. Leave the glass door and the outer door open and secure them in this state. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 131: Chapter 8 Cleaning And Disinfection
3. Then, wipe the surfaces dry using a clean cloth. Cleaning display: CAUTION Moisture-sensitive display! Do not spray or wipe the display with cleaner. • Clean display using a dry cloth of 100% micro fiber! 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 132: Decontamination Procedures
7. Pull airbox (1/Figure 8-2) out of its seat on the water reservoir cover panel and remove it. 8. Take HEPA-filter (2/Figure 8-2) out of airbox (1/Figure 8-2) and reinstall the airbox. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 133: Wipe/Spray Disinfection
Prior to cleaning and disinfection work, disconnect the device from the power supply! • Turn the device off using the power switch. • Unplug the power connector and protect it against accidental reconnection. • Make sure the device is de-energized. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 134 “Installing the shelf system“ page 4-7. 2. Remove the airbox and the HEPA-filter. The installation and removal of the airbox and the HEPA-filter are described in section “Replacing the HEPA-filter“ page 9-7. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 135: Figure 8-2. Hepa-Filter And Airbox
4. Separate the top part from the rear part of the air duct (2/Figure 8-3) and remove it from the workspace. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 136: Figure 8-3. Air Duct
1. Spray the surfaces of the workspace, the shelving system and parts removed in previous steps with disinfectant one more time and wipe dry. 2. Allow the disinfectant to react as specified by the manufacturer. 3. Reinstall the shelf system and the removed components. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 137: Steri-Run Decontamination Routine
90 minutes. The effectiveness of the steri-run decontamination routine has been certified by independent institutes. The germ reduction efficiency achieved is equal to 10 (6-log reduction) to the ISO 11138 standard. On request, Thermo Scientific will supply information on the pertinent tests.
Page 138 After the decontamination temperature has been created, the decontamination phase of approx. 90 minutes is started. The temperature is maintained at 180 °C. Cool-down phase: approx. 8 h. The device cools down until the originally set temperature set value is reached. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 139: Activating Steri-Run
4. To air the workspace, open both device doors when the audible alarm sounds after 30 seconds. 5. Remove all samples from the workspace. 6. Drain water from the reservoir, wipe up any residual water. 7. When the audible timer alarm is sounded, close both device doors. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 140: Cancel Steri-Run
4. To interrupt Steri-Run from the status display: • Press the Stop key. • As a safety scan, the steri-run - Stop dialog window appears. Proceed with work step 2 (see section above). 160i / 250i HERA 8-10 CELL VIOS Thermo Scientific...
Page 141: Steri-Run Interruption Due To Error
• Press the End key [3]. • The error message is displayed. • If the error message is confirmed, the system returns to the main menu. Figure 8-7. Interrupting / cancelling steri-run 160i / 250i HERA Thermo Scientific CELL VIOS 8-11...
Page 142: Completing Steri-Run
If the door will be opened and closed again whilst contra-con decontamination is running, the routine returns to a phase which will ensure a continuation without faults. Note Risk of burns! Do not open the door under any circumstances! 160i / 250i HERA 8-12 CELL VIOS Thermo Scientific...
Page 143 • Electrical safety check in accordance with the relevant national regulations (e.g. BGV 3). Note Functional check: If safety devices were removed or disabled for inspections, the device must not be operated before the safety devices have been reinstalled and checked for their correct function. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 144: Chapter 9 Maintenance
Do not use a container filled with water as an isothermal container because the evaporation of water will result in a lower temperature reading. Excessive workspace temperature: Excessive workspace temperatures after the calibration can be reduced by leaving the doors open for approx. 30 seconds. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 145: Temperature Calibration Procedure
6. Temperature calibration procedure Temperature Calibration Procedure Measurement example: • Temperature set value: 37 °C Reference temperature: 36.4 °C 1. Press the Temperature Display key. • The Temperature menu (Figure 9-2) is displayed. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 146: Figure 9-2. Temperature Display Field And Temperature Selection Menu
• Press the + key. To reduce the set value: • Press the - key. 5. Accept and save the target value: • Press the Enter key. • Press the Save key. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 147: Preparing The Co2 Calibration
4.0% CO or more. Calibration should be performed with the CO set value designated for the work process (prospective work process value). 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 148: Co2 Calibration Procedure
1. Press the CO display key. • The CO menu is displayed. 2. To exit the CO menu: • Press the End key [3]. 3. To enter the Calibration submenu: • Press the Calibration key. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 149: Replacing The Hepa-Filter
Steps for replacing the HEPA-filter: 1. Power down the unit, shut off the gas supply and allow the workspace to vent. 2. Take the center and bottom insert out of the workspace. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 150: Figure 9-6. Removing The Airbox
3. Lift the water reservoir cover at the front side (1/Figure 9-6). Figure 9-6. Removing the airbox 4. Pull the water reservoir cover out at the front (2/Figure 9-6) 5. Remove the airbox with the HEPA filter. 160i / 250i HERA CELL VIOS Thermo Scientific...
Page 151: Replacing The Gas Inlet Filter
6 months. Replacing the gas inlet filter The gas inlet filter (CO supply) has a plastic thread and is screwed by hand into the female thread socket at the control box. 160i / 250i HERA Thermo Scientific CELL VIOS...
Page 152: Device Fusing Replacement
Figure 9-8. Gas inlet filter installation Device fusing replacement The device’s fuses are not user-replaceable. If the unit fails due to an electrical fault, call Technical Field Service. 160i / 250i HERA 9-10 CELL VIOS Thermo Scientific...
Page 153: Replacing The Door Seal
Maintenance Replacing the Door Seal Replacing the Door Seal Note We recommend having the door seal replaced by a service technician or by expert staff. 160i / 250i HERA Thermo Scientific CELL VIOS 9-11...
Page 154 Maintenance Replacing the Door Seal 160i / 250i HERA 9-12 CELL VIOS Thermo Scientific...
Page 155: Chapter 10 Waste Disposal
Plastic components, general ABS and PPS GF40, note material identification Exterior housing Galvanized and painted steel sheet, Stainless steel 1.4016 Incubator rear panel Galvanized steel sheet 160i / 250i HERA Thermo Scientific CELL VIOS 10-1...
Page 156 HEPA-filter, micro-fine glass, Gas filter with polypropylene casing and GF/PTFE membrane, Pre-filter, stainless steel 1.4401 wire mesh Packaging Corrugated cardboard, polyethylene foil, styrofoam shaped elements and polypropylene Door seal magnet Permanent magnet 160i / 250i HERA 10-2 CELL VIOS Thermo Scientific...
Page 157: Chapter 11 Specifications
Specifications Content • “HERACELL VIOS 160i“ page 11-2 • “HERACELL VIOS 250i“ page 11-6 160i / 250i HERA Thermo Scientific CELL VIOS 11-1...
Page 158 Specifications HERACELL VIOS 160i HERA 160i CELL VIOS Name Unit Value Mechanical Outer dimensions (W x H x D) 637 x 905 x 790 (25.08 x 35.63 x 31.10 inch) Inner dimensions (W x H x D) 470 x 607 x 576 (18.50 x 23.90 x 22.68 inch)
Page 159 Specifications HERACELL VIOS 160i Name Unit Value Constant humidity at 37 °C (high humidity mode) approx. 93 Constant humidity at 37 °C (low humidity mode) approx. 90 Others Sound pressure level (DIN 45 635, part 1) dB(A) < 50 Relative ambient humidity max.
Page 160 Specifications HERACELL VIOS 160i HERA 160i CELL VIOS Name Unit Value gas supply system Gas purity min. 99.5 or med. quality Primary pressure min. 0.8 - max. 1 (11.6-14.5 psi) Measurement and control range Vol -% 0...20 Control deviation over time Vol -% ±...
Page 161 Specifications HERACELL VIOS 160i Name Unit Value Rated power 230 V: Decontamination: 1.06 Incubation: 0.56 220 V: Decontamination: 0.97 Incubation: 0.51 120 V: Decontamination: 1.01 Incubation: 0.55 100 V: Decontamination: 0.72 Incubation: 0.39 EMV class 160i / 250i HERA Thermo Scientific...
Page 162: Heracell Vios 250I
Conductivity: 1 to 20 µS/cm Capacities: max. 3 / min 0.5 Incubation operation Constant humidity at 37 °C (high humidity mode) approx. 93 Constant humidity at 37 °C (low humidity mode) approx. 90 160i / 250i HERA 11-6 CELL VIOS Thermo Scientific...
Page 163 Relative ambient humidity max. 80 Altitude of site m above sea max. 2000 level determined on the basis of DIN 12880 for devices with standard feature set. See calibration instructions for detailed information. 160i / 250i HERA Thermo Scientific CELL VIOS 11-7...
Page 164 Pollution degree (EN 61010) Rated current 230 V: Decontamination: 5.5 Incubation: 3.3 220 V: Decontamination: 5.3 Incubation: 3.2 120 V: Decontamination: 10.4 Incubation: 6.3 100 V: Decontamination: 8.9 Incubation: 5.3 Circuit breaker 16 A 160i / 250i HERA 11-8 CELL VIOS Thermo Scientific...
Page 165 Name Unit Value Rated power 230 V: Decontamination: 1.26 Incubation: 0.76 220 V: Decontamination: 1.16 Incubation: 0.69 120 V: Decontamination: 1.25 Incubation: 0.75 100 V: Decontamination: 0.89 Incubation: 0.53 EMV class 160i / 250i HERA Thermo Scientific CELL VIOS 11-9...
Page 166 Specifications HERACELL VIOS 250i 160i / 250i HERA 11-10 CELL VIOS Thermo Scientific...
Page 167: Chapter 12 Annex: Data Communication
• “Examples of data logger codes“ page 12-15 • “Program HERACELL VIOS 160i / HERACELL VIOS 250i“ page 12-21 USB interface The units come equipped with a USB interface. The USB interface complies with Standard USB 1.1 / USB 2.0 / USB 3.0 (full speed). The USB port is operated as a virtual COM port. Therefore, the port transmission speed can be changed within the defined baud rates (9,600, 19,200, 38,400, 57,600 baud.
Page 168: Installing The Usb Port Driver
As soon as the Windows Hardware Detector has identified the USB port, the Find New Hardware Wizard dialog box opens. 1. Choose the INSTALL FROM A LIST OR SPECIFIC LOCATION (ADVANCED) option. 160i / 250i HERA 12-2 CELL VIOS Thermo Scientific...
Page 169 4. On the data CD, select the DRIVER sub-directory. 5. The installation routine installs the driver: EVAL22 Board USB. After the installation has been completed successfully, the routine is completed with FINISH. 160i / 250i HERA Thermo Scientific CELL VIOS 12-3...
Page 170: Structure Of The Command Sequences For Data Communication
=amount of payload in this telegram (00 – ff ) cc = checksum: Inverted XOR of all bytes without checksum and <CR> XXXX = bb-bytes user data Response with an error message: Response: !:aaaa:bb:XX:cc<CR> 160i / 250i HERA 12-4 CELL VIOS Thermo Scientific...
Page 171: Overview Of General Parameters (Addresses 0Xxx)
Overview of incubator parameters (addresses 2xxx) Incubator parameters are divided into: • the basic parameters of the three control loops temperature, CO and O • the internal function parameters of operating functions and data logging. 160i / 250i HERA Thermo Scientific CELL VIOS 12-5...
Page 172: Reading Basic Parameters
Low humidity indicator (1 active, 0 2 bytes / hexadecimal inactive) with the format xx Example of unit status and (error) status control loops (for details see error messages) All values have two decimal places. 160i / 250i HERA 12-6 CELL VIOS Thermo Scientific...
Page 173: Reading Internal Function Parameters
Send the next 7 entries. Set reading pointer automatically to next new entry, read up to 7 entries max. Re-send entries of last telegram. Can be used after communication error has occurred. 160i / 250i HERA Thermo Scientific CELL VIOS 12-7...
Page 174: Error Memory Structure
Therefore, a response consists of 1+(10 x 2) = 21 data bytes plus separator. A data set always delivers the date, the time, the faulty control loop, the device status, and the error message. 160i / 250i HERA 12-8 CELL VIOS Thermo Scientific...
Page 175: Error Memory Data Set Structure Scheme
Therefore, the following information is transmitted in this data set: • Created on 1 January 2006 at 15:55:34 hrs. • A device error has occurred and the set temperature is too high. 160i / 250i HERA Thermo Scientific CELL VIOS 12-9...
Page 176: Overview Of The Possible Error Messages In Hex Coding
Sensor breakage 0x0002 Actual value high 0x0004 Actual value low 0x0008 Actual value not plausible 0x0010 Calibration values too high/low control loop error status 0x0001 Sensor breakage 0x0002 Actual value high 160i / 250i HERA 12-10 CELL VIOS Thermo Scientific...
Page 177: O2 Control Loop And Water Level
Set the data logger pointer to the oldest entry and output the first data sets. Query: ?:2401:00::cc<CR> Output of the following data sets, the reading pointer automatically moves gradually from the older entries to the current entries. Query: ?:2402:00::cc<CR> 160i / 250i HERA Thermo Scientific CELL VIOS 12-11...
Page 178 !:2400:e0:010b01060f3700000177002800d40000110b01060f3800000172003200d20352..:80 First data set !:2400:e0:010b01060f3700000177002800d4000011 (consisting of 32 byte ASCII characters) Second data set 0b01060f3800000172003200d20352..:80 (beginning of the second data set after 32 bytes of the first data set) 160i / 250i HERA 12-12 CELL VIOS Thermo Scientific...
Page 179: Data Logger Data Set Structure Scheme
• the temperature is 37.5°C, • gas concentration 4.0% CO , 21.2% O Note Example of a code: For an example of a code, please refer to the end of this chapter. 160i / 250i HERA Thermo Scientific CELL VIOS 12-13...
Page 180: Overview Of Possible Event Entries In Bit Coding
O value (2 bytes each) 0x50 Start auto-start Status / error registration for temperature, CO and rH 0x51 auto-start successfully completed Current actual values for temperature, CO and rH 160i / 250i HERA 12-14 CELL VIOS Thermo Scientific...
Page 181: Examples Of Data Logger Codes
2nd byte: day of entry 3rd byte: month 4th byte: year 5th byte: hours 6th byte: minutes 7th and 8th byte: device status 9th to 16th byte: various data on the event 160i / 250i HERA Thermo Scientific CELL VIOS 12-15...
Page 182: Functions For Data Logger Queries
// calculate checksum: inverted XOR of all bytes // without checksum and <CR> for (i = 0; i < 11; i ++) bcc = (bcc^string[i]); // copy checksum string[11] = hexa(bcc/16); string[12] = hexa(bcc%16); 160i / 250i HERA 12-16 CELL VIOS Thermo Scientific...
Page 183 = 0; l = 0; // Negative number? if (z < 0) { // set algebraic sign b[0] = ‘-’;l = 1; // convert value z = 0xffffffff-z+1; // store decimal place 160i / 250i HERA Thermo Scientific CELL VIOS 12-17...
Page 184 (1) // set data logger to beginning and read if (!GetTele) { send_telegramm („2400“); else{ // read additional data sets send_telegramm („2401“); len = get_telegramm (buffer); // no telegram received 160i / 250i HERA 12-18 CELL VIOS Thermo Scientific...
Page 185 (status & INFO_ERROR){ str_cpy (&excelstring[len], „Error active;“, 13); len += 13; else{ // query all device errors (see “Overview of possible event entries in bit coding Overview of event 160i / 250i HERA Thermo Scientific CELL VIOS 12-19...
Page 186 ++] = ‘;’; len += num_2_str (SollO2, &excelstring[len]); excelstring[len ++] = ‘;’; len += num_2_str (SollrH, &excelstring[len]); excelstring[len ++] = ‘;’; excelstring[len] = ‘\n’; len += 1; WriteFile (FileHandle, excelstring, len); break; 160i / 250i HERA 12-20 CELL VIOS Thermo Scientific...
Page 187: Program Heracell Vios 160I / Heracell Vios 250I
Annex: Data communication Program HERACELL VIOS 160i / HERACELL VIOS 250i // query the remaining events from here case EVENT_FORMAT_DATALOG: WriteFile (FileHandle, excelstring, len); WriteFile (FileHandle, „Data logger erased;\n“,20); break; case EVENT_POWER_ON: // update of set values SetTemp = numeric string [8+i*SIZE_DATA2]*0x100+ numeric string[9+i*SIZE_DATA2];...
Page 188: Installing The Heracell Vios 160I & Heracell Vios 250I
Annex: Data communication Program HERACELL VIOS 160i / HERACELL VIOS 250i • Reading and archiving error messages (error logger). The data sets are stored in the meta format *.CSV. • Reading and archiving event entries (data logger). The data sets are stored in the meta format *.CSV.
Page 189: Operating The Heracell Vios 160I & Heracell Vios 250I
Annex: Data communication Program HERACELL VIOS 160i / HERACELL VIOS 250i 160i & HERA HERA 250i CELL VIOS CELL VIOS Operating the User menu structure: The user surface is divided into two main menus: • MAIN with two functional elements: •...
Page 190 Annex: Data communication Program HERACELL VIOS 160i / HERACELL VIOS 250i Function of the user menu: PRESETTING The submenu PRESETTING is used to set the transmission speed and to select the serial port. 1. Select a transmission speed within the range of 9,600 to 115,200 baud.
Page 191 Annex: Data communication Program HERACELL VIOS 160i / HERACELL VIOS 250i TEST COM The submenu TEST COM is used for testing the communication connection with the settings defined in the submenu PRESETTING. 1. Example of a query for the currently measurable temperature values of the incubator: •...
Page 192 Annex: Data communication Program HERACELL VIOS 160i / HERACELL VIOS 250i DATE & TIME The submenu DATE & TIME is used for adjusting date and time to the desired time zone. 1. Data in the two text boxes must be entered in the format DD.MM.YY (day, month, year).
Page 193 Annex: Data communication Program HERACELL VIOS 160i / HERACELL VIOS 250i The data sets can be stored in the metaformat *.CSV. 1. Read the data sets: • Press the READ key. 2. The progress of the data transmission is indicated in the three text boxes: •...
Page 194 Annex: Data communication Program HERACELL VIOS 160i / HERACELL VIOS 250i PASSWORD The submenu PASSWORD can only be accessed by the service personnel of Thermo Fisher Scientific. 160i / 250i HERA 12-28 CELL VIOS Thermo Scientific...
Page 195: Chapter 13 Device Log
Device log Please list carried out works here: Device type: Part No.: Serial number: Service number: Location: Operator’s note: Work carried out: Comments: Date Signature 160i / 250i HERA Thermo Scientific CELL VIOS 13-1...
Page 196 Device log 160i / 250i HERA 13-2 CELL VIOS Thermo Scientific...
Page 197: Chapter 14 Contact Information Thermo Scientific
Contact Information Thermo Scientific Overview of the Thermo Fisher international sales organisations postal address Germany: Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Enquiries from Germany: Phone Sales 0800 1 536376 Phone Service 0800 1 112110 Fax Sales/Service 0800 1 112114 Email info.labequipment.de@thermofisher.com...
Page 198 Contact Information Thermo Scientific Enquiries al address USA: Thermo Scientific 275 Aiken Road Asheville, NC 28804 Enquiries from USA/Canada +1 866 984 3766 Sales: Service+1 800 438 4851 Enquiries from Latin America +1 866 984 3766 Sales: Service:+1 866 984 3766...
Page 199 Contact Information Thermo Scientific Germany: Postal Address Germany: Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Phone Sales Toll free 0800 1 536 376 or +49 6184 90 6940 Service Toll free 0800 1 112110 or +49 6184 90 6940 E-Mail info.labequipment.de@thermofisher.com...
Page 200 Contact Information Thermo Scientific Thermo Fisher Scientiﬁc, San Jose, CA USA is ISO Certiﬁed. thermoscienti c.com © 2013 Thermo Fisher Scientiﬁc Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientiﬁc Inc. and its subsidiaries. Speciﬁcations, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.

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Heracell vios 250i

Thermo Scientific HERACELL VIOS 160i User Manual

Chapter 1 Delivery of the Incubator

Chapter 2 Installation of the Device

Chapter 3 Description of the Device

Chapter 4 Start-Up

Chapter 5 Operation

Chapter 6 Handling and Control

Chapter 7 Shut-Down

Chapter 8 Cleaning and Disinfection

Chapter 9 Maintenance

Chapter 10 Waste Disposal

Chapter 11 Specifications

Chapter 12 Annex: Data Communication

Chapter 13 Device Log

Chapter 14 Contact Information Thermo Scientific

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