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Original operating manual 07/2012 Rev. 1.26 of 24/10/2023 Sigma 4-16KS; ; part no. 0701702 Sigma 4-16KS from serial no. 144061 Refrigerated Centrifuge Operating Manual Please retain for later use!
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Refrigerated Centrifuge Sigma 4-16KS Version 07/2012, Rev. 1.26 of 24/10/2023 • sb 2 / 99 Original operating manual, part no. 0701702...
3.6.7.1 Marking of rotors and accessories ..............23 3.6.7.2 Service life of rotors and accessories ..............24 3.6.7.3 Service life of the Sigma "Comfort" rotor coating ..........25 Safety devices ........................ 27 3.7.1 Lid lock device ......................27 3.7.2 Standstill monitoring system ..................27 3.7.3...
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Refrigerated Centrifuge Sigma 4-16KS Table of contents Storage and transport ....................... 29 Dimensions and weight ....................29 Storage conditions ......................29 Notes on transport ......................29 Packaging ........................30 Transport safety device ....................30 Set-up and connection ...................... 32 Installation site ........................ 32 Power supply ........................
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Refrigerated Centrifuge Sigma 4-16KS Table of contents Malfunctions and error correction ..................63 General malfunctions ...................... 63 7.1.1 Emergency lid release ....................64 Table of error codes ......................65 Service contact ....................... 66 Maintenance and service ....................67 Maintenance ........................67 8.1.1...
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Refrigerated Centrifuge Sigma 4-16KS Table of contents Version 07/2012, Rev. 1.26 of 24/10/2023 • sb 8 / 99 Original operating manual, part no. 0701702...
Refrigerated Centrifuge Sigma 4-16KS 3 Safety Pos : 34 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0020 Symbol- und Hi nweis erklär ung en @ 26\mod_1405319013955_68.docx @ 191947 @ 2 @ 1 → Explanation of the symbols and notes...
Refrigerated Centrifuge Sigma 4-16KS 3 Safety Pos : 36 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0030 Ver antw ortung des Betrei bers @ 26\mod_1405319015364_68.docx @ 191961 @ 2 @ 1 → Responsibility of the operator The operator is responsible for authorising only qualified personnel to work on the centrifuge (see chapter 3.4 - "Requirements concerning the...
Refrigerated Centrifuge Sigma 4-16KS 3 Safety Pos : 38 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0040 Pers onalanforderungen @ 26\mod_1405319017634_68.doc x @ 191989 @ 2 @ 1 → Requirements concerning the personnel Risk of injury if the personnel are not sufficiently qualified...
Refrigerated Centrifuge Sigma 4-16KS 3 Safety Pos : 40 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0050 Informelle Sic herheits hinw eise @ 26\m od_1405319019966_68.docx @ 192017 @ 2 @ 1 → Informal safety instructions • This operating manual is a part of the product.
Leerz eile @ 0\m od_1202116244500_0.doc x @ 114 @ @ 1 Pos : 45 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0020 M ec hanisc he Sic her heit (auß er 1-16/1-16K/1-7) @ 26\mod_1405319024314_68.docx @ 192059 @ 3 @ 1 →...
Leerz eile @ 0\m od_1202116244500_0.doc x @ 114 @ @ 1 Pos : 49 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0040 C hemische und bi ologisc he Sic her hei t @ 26\m od_1405319028141_68.docx @ 192101 @ 3 @ 1 →...
Refrigerated Centrifuge Sigma 4-16KS 3 Safety Pos : 51 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0050 Sicherheits hi nweis e z ur Z entrifug ation @ 26\mod_1405319029632_68.doc x @ 192115 @ 3 @ 1 → 3.6.5 Safety instructions for centrifugation For safe operation, observe the following before starting the centrifuge: •...
Safety of rotors and accessories Pos : 56 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0070- 0010 Kennz eichnung v on R otoren und Z ubehör Mas chR L 4er+ 6er @ 105\m od_1695969338260_68.docx @ 1224058 @ 4 @ 1 3.6.7.1...
3 Safety Pos : 58 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0070- 0020 Lebens dauer Rotor en und Zubehör M asc hRL - Übersc hrift neutr al @ 83\m od_1622527518082_68.docx @ 878477 @ 4 @ 1 3.6.7.2 Service life of rotors and accessories Pos : 59 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0070- 0020 Lebens dauer Rotor en und Zubehör M asc hRL - Text @ 26\mod_1405319032612_68.docx @ 192143 @ @ 1...
Refrigerated Centrifuge Sigma 4-16KS 3 Safety Pos : 61 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0070- 0040 Lebens dauer Sigma C omfort R otorbesc hic htung @ 105\m od_1695375251961_68.docx @ 1222758 @ 4 @ 1 3.6.7.3 Service life of the Sigma "Comfort" rotor coating Marking ll "Comfort"...
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Refrigerated Centrifuge Sigma 4-16KS 3 Safety Traces of use on the black coating do not affect the anti-friction properties (see the following illustration). NOTE Fig. 7: Load-bearing bolt with traces of use – no greasing required End of the service life of the "Comfort" rotor coating...
Refrigerated Centrifuge Sigma 4-16KS 3 Safety Pos : 78 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0080 Ver halten bei Gefahren und Unfäll en @ 27\mod_1405319051188_68.docx @ 192325 @ 2 @ 1 → Measures in the event of hazards and accidents •...
Transport safety device Pos : 93 /100 Sigma/110 BA Zentrifugen Sigma (Pr ojekte) /4- 16S/040 Lagerung und Tr ans port/040-0051 Trans portsic herung 4- 16S_4- 16KS_4-16KH S - T ext @ 36\m od_1434606412183_68.docx @ 263977 @ @ 1 The transport safety device consists of two plastic screws. They are located on the base plate and can be accessed from below.
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Refrigerated Centrifuge Sigma 4-16KS 4 Storage and transport Locking screws Fig. 9: Unscrew the locking screws by turning them anti-clockwise • Retain the transport safety device for the possibility of the return of the centrifuge. Pos : 94 /010 U nivers almodule/ Absc hnittsw echs el @ 0\m od_1202124514062_0.docx @ 418 @ @ 1...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 115 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/060- 0020-0020-0023 Eins etzen eines Wi nk elrotors mit H ermetikdeck el 10 Nm @ 29\m od_1405326759765_68.docx @ 198499 @ 4 @ 1 6.2.2.2...
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Removing a rotor • Loosen the rotor tie-down screw anti-clockwise and remove the rotor together with the lid. • Loosen the lid by turning it anti-clockwise and then remove it. Rotor tie down screw Rotor Fig.
6 Using the centrifuge Pos : 117 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/060- 0020-0020-0032 Eins etzen v on Z ubehör 4+6plätzig 4- 16S, 4- 16KS/KH S @ 27\mod_1405319207709_68.doc x @ 192535 @ 4 @ 1 →...
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Centrifugation with low capacity • Install the sample vessels in a rotationally symmetrical manner so that the buckets and their suspensions are loaded evenly. • It is not permissible to load angle rotors on only one axis.
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 133 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0040 Auswahl, Anzeig e und Änderung von D aten @ 27\m od_1405320202914_68.docx @ 193207 @ 4 @ 1 →...
6 Using the centrifuge Pos : 135 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0050 M enü Standard -nur KS @ 27\mod_1405320204084_68.docx @ 193221 @ 4 @ 1 →...
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Runtime The set runtime is displayed in the upper section of this field, with the remaining runtime shown below. The runtime is defined as the period from the start of the centrifuge to the beginning of the deceleration phase. The maximum value is 99 h 59 min 59 sec.
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Rotor: rotor selection list This field shows the rotor that is currently being used. • Select the field "Rotor" and confirm the selection. A list with all of the possible rotors without buckets is displayed.
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Acceleration This function is used to select an acceleration curve. One can select a linear rise (curves 0-9) or a quadratic rise (curves 10-19). The acceleration curves 20-29 can be programmed as desired (see chapter 11.3 - "Acceleration and deceleration curves").
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Fig. 19: Program "RAPID_TEMP" The program will only be loaded if the actual temperature is above the set temperature. NOTE • Press the start button in order to start the rapid cooling run.
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge If the "RAPID_TEMP" program is used, the temperature of the unloaded aluminium bucket will be displayed. If samples, which have not been precooled, are placed into the buckets, the displayed temperature will deviate from the actual sample temperature.
6 Using the centrifuge Pos : 139 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0060 M enü Param eter - KS_KBS @ 27\mod_1405320218640_68.docx @ 193305 @ 4 @ 1 →...
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Density This setting is useful for glass vessels. If the density of the liquid to be centrifuged is higher than 1.2 g/cm , the value must be adapted manually in order to prevent the glass vessel from breaking. This will reduce the maximum possible final speed (see chapter 2.2.2.2 - "Density").
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Temperature monitoring The control system includes a temperature monitoring function. If the actual temperature difference with regard to the set value deviates from the set temperature difference, the centrifuge will stop and an error message will be issued.
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Lock In order to prevent any unauthorised use of the centrifuge, the following functions can be blocked: • Saving of programs (level 01) • Changing of parameters, short run (level 02) •...
Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Pos : 141 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0070 M enü Setup @ 27\mod_1405320225479_68.docx @ 193361 @ 4 @ 1 →...
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge Start delay If the start delay function is active, the centrifuge will not start until the preset time has elapsed. The symbol "" will be displayed in the runtime field. Display deceleration time In the activated mode, the deceleration time will be displayed instead of the runtime during and after the deceleration process.
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Refrigerated Centrifuge Sigma 4-16KS 6 Using the centrifuge System Cycles This field shows the number of cycles as well as the runtime of the rotor and buckets that are used. Info This item provides information on the software versions that are used in this centrifuge.
6 Using the centrifuge Pos : 151 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0030-0010 Programm speic her n @ 27\m od_1405320237832_68.docx @ 193487 @ 4 @ 1 →...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 157 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0030-0040 Programm l ösc hen @ 27\mod_1405320245137_68.docx @ 193557 @ 4 @ 1 →...
6 Using the centrifuge Pos : 159 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0030-0050 Autom atis che Programmrotation @ 27\m od_1405320248329_68.docx @ 193585 @ 4 @ 1 →...
Pos : 161 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0040 Opti onen z ur Ein- und Ausgabe von D aten (auß er 8KS) @ 27\m od_1405320251483_68.docx...
Refrigerated Centrifuge Sigma 4-16KS 7 Malfunctions and error correction Pos : 171 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/070 Störungen und F ehl ersuche/070-0020 T abell e der F ehlerm eldung en @ 28\mod_1405320582996_68.docx @ 194761 @ 2 @ 1 →...
Refrigerated Centrifuge Sigma 4-16KS 8 Maintenance and service Pos : 176 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080 Wartung und Instandhaltung ========================= @ 28\m od_1405320612731_68.docx @ 194789 @ 1 @ 1 → Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress.
Pos : 189 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0010- 0040-0010 Trag bolz en mit C omfort R otorbesc hichtung (auß er 2-7, 2- 16P, 2-16KL) @ 90\mod_1651133147973_68.docx @ 949768 @ 4 @ 1...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 193 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0020 Sterilisati on und Desinfek tion v on Rotork ammer und Zubehör @ 28\m od_1405320626090_68.docx @ 194929 @ 2 @ 1 →...
Refrigerated Centrifuge Sigma 4-16KS 8 Maintenance and service Pos : 195 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0020- 0010 Autoklavi eren @ 28\mod_1405320627440_68.doc x @ 194943 @ 3 @ 1 → 8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use.
Refrigerated Centrifuge Sigma 4-16KS 8 Maintenance and service Pos : 197 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0030 Ins tandhaltungs arbeiten @ 28\mod_1405320628810_68.docx @ 194957 @ 2 @ 1 → Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury.
Refrigerated Centrifuge Sigma 4-16KS 8 Maintenance and service Pos : 199 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0040 R ücks endung defekter T eile @ 28\mod_1405320630226_68.doc x @ 194971 @ 2 @ 1 →...
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NOTE The forms can be downloaded online from www.sigma-zentrifugen.de → [Service] → [Overhaul and repair]. Pos : 200 /010 Univ ers almodul e/ Abs chnittswec hs el @ 0 \mod_1202124514062_0.docx @ 418 @ @ 1 Pos : 201 /010 Univ ers almodul e/ Seitenwechs el @ 0\mod_1202116244312_0.docx @ 105 @ @ 1...
10 Technical data Pos : 209 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/100 T ec hnisc he D aten/100 T ec hni sche D aten - Ü bersc hrift ============================= @ 7\mod_1306745782782_68.doc x @ 43932 @ 1 @ 1 →...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 214 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/100 T ec hnisc he D aten/100- 0020 Technisc he Dok um entation @ 28\m od_1405320681369_68.docx @ 195055 @ 2 @ 1 →...
→ 11 Appendix Pos : 218 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0010 Z ubehör programm (ohne T abell e, mit Hinw eis Beil agebl ätter) @ 56\m od_1523438203637_68.docx @ 560428 @ 2 @ 1 → 11.1...
Refrigerated Centrifuge Sigma 4-16KS 11 Appendix Pos : 225 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0031 Beschl eunigungs- und Bremsk urven Spinc ontrol S+Z ent2 @ 28\mod_1405320708834_68.docx @ 195181 @ 2 @ 1 → 11.3 Acceleration and deceleration curves Linear as well as quadratic curves are numbered in the direction of increasing acceleration (from right to left).
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Refrigerated Centrifuge Sigma 4-16KS 11 Appendix Fig. 42: Diagram of linear curves Fig. 43: Diagram of quadratic curves Pos : 226 /010 Univ ers almodul e/ Seitenwechs el @ 0\mod_1202116244312_0.docx @ 105 @ @ 1 Version 07/2012, Rev. 1.26 of 24/10/2023 • sb 82 / 99 Original operating manual, part no.
Refrigerated Centrifuge Sigma 4-16KS 11 Appendix Pos : 227 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0040 T abell e: Lebensdauer v on R otoren und Z ubehör @ 28\mod_1405320709911_68.docx @ 195195 @ 2 @ 1 → 11.4 Table of the service life of rotors and accessories •...
Refrigerated Centrifuge Sigma 4-16KS 11 Appendix 11.8 UKCA declaration of conformity Version 07/2012, Rev. 1.26 of 24/10/2023 • sb 93 / 99 Original operating manual, part no. 0701702...
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Refrigerated Centrifuge Sigma 4-16KS 11 Appendix === Ende der Liste für T extm arke Inhalt === Version 07/2012, Rev. 1.26 of 24/10/2023 • sb 94 / 99 Original operating manual, part no. 0701702...
Refrigerated Centrifuge Sigma 4-16KS Index 12 Index Carrier systems .......... 39 Carriers, cleaning and care ......69 Acceleration ..........45 CE mark in compliance with the directive Acceleration curve ......45, 56, 81 2006/42/EC ..........15 Acceleration curve (changing) ....56 Centrifugation monitoring ......
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Refrigerated Centrifuge Sigma 4-16KS Index Cracking ............ 68 Executing a program ........60 Cracks ............69 Explanation of the symbols and notes ..16 Creating an acceleration curve ....56 Explosive substances ........ 21 Curve menu ..........56 External signal ........... 55 Customer-provided fuses ......
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Refrigerated Centrifuge Sigma 4-16KS Index Installation of an angle rotor with a hermetic Menu Processlibrary ........48 lid ............35 Menu Setup ..........53 Installation of rotors and accessories ..34 Menu Standard .......... 42 Installation site ........... 32 Mode of operation ........13 Insufficiently greased load-bearing bolts ..
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Return of defective parts ......74 Service work ..........73 Rotor chamber ........... 67 Set-up and connection ....... 32 Rotor coating Sigma "Comfort" ....25 Setup menu ..........53 Rotor identification, automatic ....44 Short run ............ 43 Rotor monitoring system ......27 Sigma ..........
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Refrigerated Centrifuge Sigma 4-16KS Index Standstill monitoring system ...... 27 Temperature monitoring system ....27 Start delay..........54 Temperature range ........77 Starting a centrifugation run ....... 41 Temperature unit........54 Starting a process ........48 Temperature value not reached ....63 Sterilisation of the rotor chamber and Thermal stress ...........
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