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Thermo Scientific ERGOSTAR HM 200 Instruction Manual
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Thermo Scientific ERGOSTAR HM 200 Instruction Manual

Section transfer system microm sts for rotary microtomes
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Thermo Scientific Section Transfer System Microm STS
Thermo Scientific
SECTION TRANSFER SYSTEM Microm STS
For ROTARY MICROTOMES / ERGOSTAR HM 200
387712 Issue 8
INSTRUCTION MANUAL
Microm International GmbH
1
Part of Thermo Fisher Scientific
Im Steingrund 4 - 6
63303 Dreieich / Germany
387 712 - English

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Summary of Contents for Thermo Scientific ERGOSTAR HM 200

  • Page 1 Thermo Scientific Section Transfer System Microm STS Thermo Scientific SECTION TRANSFER SYSTEM Microm STS For ROTARY MICROTOMES / ERGOSTAR HM 200 387712 Issue 8 INSTRUCTION MANUAL Microm International GmbH Part of Thermo Fisher Scientific Im Steingrund 4 - 6 63303 Dreieich / Germany...

  • Page 2: Warranty

    Thermo Scientific Section Transfer System Microm STS CERTIFICATION Thermo Fisher Scientific Microm International GmbH certifies that this instrument has been tested and checked carefully. Its technical data was verified before shipment to be in accordance with the published specifications. The instrument complies with applicable international safety regulations.

  • Page 3: Intended Use

    Thermo Scientific Section Transfer System Microm STS INTENDED USE Dear Customer, Thank you for buying this Thermo Scientific instrument. Before putting the instrument into operation, please read these operating instructions carefully to familiarize yourself with its proper operation and functions.

  • Page 4: Part 5 Maintenance

    Thermo Scientific Section Transfer System Microm STS Intended use Safety precautions PART 1 INTRODUCTION Description of the Section Transfer System STS Technical data Section Transfer System PART 2 OPERATING INSTRUCTIONS Initial turn-on Trim sectioning of specimens Fine sectioning of specimens...

  • Page 5: Safety Precautions

    Thermo Scientific Section Transfer System Microm STS SAFETY PRECAUTIONS WARNING SIGNALS AND SYMBOLS The installation and routine use of the STS is easy and safe if the instructions in this manual are being observed. However, the situations which might be risky for the personnel or equipment are specially marked in this manual with the following symbols and messages.

  • Page 6: Instrument Grounding

    Thermo Scientific Section Transfer System Microm STS SAFETY PRECAUTIONS A T T E N T I O N ! Please observe the following general precautions during operation of this instrument. Failure to comply with these precautions violates safety standards and the intended use of the instrument.
  • Page 7 Thermo Scientific Section Transfer System Microm STS HAZARD OF WATER Set up and operate the separate control unit in a way that it is protected from water. HAZARD OF BIOLOGICAL DANGER Specimens used during the intended operation of the instrument might potentially be infectious. For this reason, it is recommended to observe the general laboratory regulations concerning protection against danger of infection.
  • Page 8 Thermo Scientific Section Transfer System Microm STS Fig. 1 Microm International GmbH Part of Thermo Fisher Scientific Im Steingrund 4 - 6 63303 Dreieich / Germany 387 712 - English...
  • Page 9 Thermo Scientific Section Transfer System Microm STS INTRODUCTION Description of the Section Transfer System STS The system consists of a special disposable blade carrier TE with integrated transfer bridge, a heated water bath as well as a control unit. The Section Transfer System can be used along with all current rotary microtomes and the Ergostar HM 200.
  • Page 10 Thermo Scientific Section Transfer System Microm STS TECHNICAL DATA SECTION TRANSFER SYSTEM Heated water bath: +45°C Volume of heated water bath: 800 ml Volume of pump reservoir: 600 ml Required liquid: demineralized or distilled water Flow rate: 0 ml/min. - 500 ml/min.
  • Page 11 Thermo Scientific Section Transfer System Microm STS OPERATING INSTRUCTIONS Initial turn-on Note: The work sequence which is described in this part must strictly be observed. To use a rotary microtome together with a Section Transfer System (STS), following preparations are necessary: ...
  • Page 12 Thermo Scientific Section Transfer System Microm STS Note: The control unit must be positioned in a way that the ventilating slots are not covered. The optimal as well as space- saving position is on top of the microtome housing. Moreover, please note that there is always enough space behind the control unit to easily the mains plug unit to the supply.
  • Page 13 Thermo Scientific Section Transfer System Microm STS  Connect the plug of the system into the power outlet.  Turn on the STS via the touchpad key "0/1" MICROM (fig. 6.5) on the control unit. Adjust the set temperature of the water...
  • Page 14 Thermo Scientific Section Transfer System Microm STS  Via the lateral adjusting wheel increase the pump capacity that there is an even water flow on the transfer surface (fig. 8.4).  The pump capacity is too high, in case water flows down behind the blade on the disposable blade carrier TE.
  • Page 15 Thermo Scientific Section Transfer System Microm STS Trim sectioning of specimens For the first cuts, approach specimen and disposable blade carrier TE as usual.  Set the water volume running down the disposable blade carrier, on the lateral adjusting wheel (fig. 2.1) depending on the selected trimming thickness.
  • Page 16 Thermo Scientific Section Transfer System Microm STS Fine sectioning of specimens The STS offers two different possibilities to evaluate the quality of the sections produced In general, both single sections as well as and to make a selection: ribbons can be generated with the STS.
  • Page 17 Thermo Scientific Section Transfer System Microm STS Draining and cleaning the water bath and the pump reservoir Draining the water bath  For this remove the water bath (fit. 5.1) from the heating plate (fig. 5.2). The heating as well as the section illumination are deactivated via the inductive sensor.
  • Page 18 Thermo Scientific Section Transfer System Microm STS Cleaning the pump filter  To clean the pump filter (fig. 5.8), pull it off.  Brush down the coarse paraffin particles according to the respective lab regulations and dispose of them.  Wipe off the finer paraffin particles with a cloth soaked in Xylene.
  • Page 19 Thermo Scientific Section Transfer System Microm STS Disposable blade carrier TE The disposable blade carrier TE is designed to take all commercially available high and low profile disposable blades. Insert the blade into the slot. When using high profile blades, first loosen the two screws and remove the spacer strip.
  • Page 20 Thermo Scientific Section Transfer System Microm STS Standard equipment The Section Transfer System is supplied with the following accessories: 1 disposable blade carrier TE 1 water bath 1 STS basic instrument including heating plate, pump and pump reservoir 1 control unit 2 transfer surface (2 parts) for replacement (see fig.
  • Page 21 Thermo Scientific Section Transfer System Microm STS PART 3 THEORY OF OPERATION OF THE SECTION TRANSFER SYSTEM Water circulation circular pump delivers distilled demineralized water from a reservoir to a blade carrier for high and low profile blades via a tube.
  • Page 22 Thermo Scientific Section Transfer System Microm STS Cutting and detaching a section manual rotary movement handwheel of a rotary microtome is converted into a vertical movement of the specimen clamping. feed movement between specimen clamping and blade carrier is carried out horizontally.
  • Page 23 Thermo Scientific Section Transfer System Microm STS PART 4 WORKING WITH THE SECTION TRANSFER SYSTEM Preparing the STS As already described in part 2-1, prepare the rotary microtome for operation with a STS. Adapt the removable water bath, put the waste receptacle onto the pump reservoir and place the waste basket on it.
  • Page 24 Thermo Scientific Section Transfer System Microm STS Sectioning and picking up sections Set the desired section thickness and start sectioning. Detailed information on sectioning can be found in part 4 of the instruction manuals of the respective rotary microtome. When the produced fine sections are evaluated as high quality sections, close the transfer bridge via the lever.
  • Page 25 Thermo Scientific Section Transfer System Microm STS Working with the STS in combination with the Cool-Cut Two different transfer surfaces for the transfer bridge on the disposable blade carrier TE are included in the standard equipment Depending on the operation with or without a Cool-Cut, these transfer surfaces must be cut appropriately.
  • Page 26 Thermo Scientific Section Transfer System Microm STS How to avoid malfunctions Caution: Turn on the STS only after having filled system with distilled demineralized water.  To fill the system, only use distilled or demineralized water to keep furring on the transfer track at a minimum.
  • Page 27 Thermo Scientific Section Transfer System Microm STS PART 5 MAINTENANCE Annual routine maintenance Caution: To secure section quality and functioning of the Any shipping of the instrument requires original STS, it is recommended that a routine packaging materials! Damages caused by...
  • Page 28 Thermo Scientific Section Transfer System Microm STS PART 6 CLEANING AND CARE CLEANING Cleaning intervals Cleaning of the STS depends on how frequently the system is used. The more it is used, the more it will need to be cleaned.
  • Page 29 Thermo Scientific Section Transfer System Microm STS To replace the bridge part of the transfer surface, first remove the water bath to have enough clearance for the replacement work. Now dismount the bridge part from the lower part of the bridge via a screwdriver and mount a new bridge part from the front.